FINAL COMMENT: Performance Andromeda/TILL

[offterdi1]

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Dear all,
We had an excellent meeting with a Till Photonics delegation. They were very
supportive and together with them we could identify and (hopefully) finallly
solve the performance problems at our instrument.
We are now fully satisfied with the offered solutions to our problems.


Best


Martin Offterdinger

Medical University of Innsbruck, Austria

[Csúcs Gábor]

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Dear Martin,

It is very nice to hear that you have found a solution for your problem,
but it would be very interesting to know what the problem was. Could you
share it with us?

Thanks     Gabor

Light Microscopy and Screening Centre
ETH Zurich, HPM G41
Schafmattstrasse 18
CH-8093, Zurich
Switzerland

Phone: [phone]
Mobile: [phone]







On 11/14/12 3:57 PM, "Martin Offterdinger"
<[hidden email]> wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>Dear all,
>We had an excellent meeting with a Till Photonics delegation. They were
>very
>supportive and together with them we could identify and (hopefully)
>finallly
>solve the performance problems at our instrument.
>We are now fully satisfied with the offered solutions to our problems.
>
>
>Best
>
>
>Martin Offterdinger
>
>Medical University of Innsbruck, Austria

[offterdi1]

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Hi
Sure, Gabor.
It seems that the choice of the objective is very crucial for the
signal-noise ratio of the system. We used to take a 60 x 1.2 NA water
immersion lens...in principal good for live cell imaging. The better choice
for this system seems to be a 60 x 1.35 NA oil lens, or even better if the
sensitivity is a problem (like in our case) a 40 x 1.35 NA oil lens. The
details we can now figure out ourselves but in principle the matter is at
least very close to a solution.
We cannot exclude other kinds of trouble due to the specific sample
preparation which is very unusual: Carbon coated coverslips, which are
addtionally coated with gelatin (this could also cause some optical
problems, but it used to work well in our demo one year ago.); on top of
this muscle cells are grown...


Best,
Martin