FW: Yokogawa lenses
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Comment from Tom Donelly. Seems like UV plain won't work!
From: Donnelly, Tom [mailto:[hidden email]] Sent: Saturday, 14 November 2009 1:35 PM To: Guy Cox Subject: Yokogawa lenses Microlenses are plastic. This email message,
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OK, from another commercial person (who does not want
to be named) I've now heard that in fact the Yokogawa microlenses are in fact
silica (as I had suspected). But the snag is that there is another optic
in the system which does not pass UV. This is a bit more promising, since
replacing the microlenses is not an option but replacing a transfer lens
shouldn't be difficult. Someone from Yokogawa must surely be reading this
list. One assumes, also, that they would be interested in
extending the application range of their system. So, come on, Yokogawa,
please give us the real story!
Guy
Optical Imaging Techniques in Cell Biology |
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It is possible that they also have to take into account the possible
heating due to intense UV ciao Alby ISTITUTO ITALIANO DI TECNOLOGIA Prof. Alberto Diaspro Scientific Head Nanophysics Via Morego, 30 16163 Genova Tel: +39-010.71.781.503 Fax +39-010-72.03.21 Mobile +39-3666719968 www.iit.it On Nov 14, 2009, at 12:55 PM, Guy Cox wrote: > OK, from another commercial person (who does not want to be named) > I've now heard that in fact the Yokogawa microlenses are in fact > silica (as I had suspected). But the snag is that there is another > optic in the system which does not pass UV. This is a bit more > promising, since replacing the microlenses is not an option but > replacing a transfer lens shouldn't be difficult. Someone from > Yokogawa must surely be reading this list. One assumes, also, that > they would be interested in extending the application range of > their system. So, come on, Yokogawa, please give us the real story! > > Guy > > > Optical Imaging Techniques in Cell Biology > by Guy Cox CRC Press / Taylor & Francis > http://www.guycox.com/optical.htm > ______________________________________________ > Associate Professor Guy Cox, MA, DPhil(Oxon) > Electron Microscope Unit, Madsen Building F09, > University of Sydney, NSW 2006 > ______________________________________________ > Phone +61 2 9351 3176 Fax +61 2 9351 7682 > Mobile 0413 281 861 > ______________________________________________ > http://www.guycox.net > > > ---------------------------------------- Alberto Diaspro Head, Nanophysics Unit Senior Scientist The Italian Institute of Technology -IIT Via Morego, 30 16163 - Genova (Italy) phone: +39 010 71781503 mobile: +393666719968 fax: +39 010 720321 http://www.iit.it [hidden email] Professor of Applied Physics Department of Physics University of Genova Via Dodecaneso, 33 16146 Genova - Italy tel. +39 010 353 6426 fax. +39 010 314218 http://www.lambs.it [hidden email] ------------------------------------------------------- |
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Alby and Guy,
sure enough heating with UV is a concern. I did wish someone from Yokogawa read this list and gave a conclusive answer whether any non UV-compatible element in the lightpath could be customized to be UV friendly. Grazie and Ciao, Renato Renato A. Mortara Disciplina de Parasitologia UNIFESP Escola Paulista de Medicina R. Botucatu, 862 6o andar 04023-062 S?o Paulo SP Brasil Quoting Alberto Diaspro <[hidden email]>: > It is possible that they also have to take into account the possible > heating due to intense UV > ciao > Alby > > > > > ISTITUTO ITALIANO > DI TECNOLOGIA > > Prof. Alberto Diaspro > Scientific Head > Nanophysics > Via Morego, 30 16163 Genova > Tel: +39-010.71.781.503 > Fax +39-010-72.03.21 > Mobile +39-3666719968 > www.iit.it > > > On Nov 14, 2009, at 12:55 PM, Guy Cox wrote: > >> OK, from another commercial person (who does not want to be named) >> I've now heard that in fact the Yokogawa microlenses are in fact >> silica (as I had suspected). But the snag is that there is another >> optic in the system which does not pass UV. This is a bit more >> promising, since replacing the microlenses is not an option but >> replacing a transfer lens shouldn't be difficult. Someone from >> Yokogawa must surely be reading this list. One assumes, also, that >> they would be interested in extending the application range of >> their system. So, come on, Yokogawa, please give us the real story! >> >> Guy >> >> >> Optical Imaging Techniques in Cell Biology >> by Guy Cox CRC Press / Taylor & Francis >> http://www.guycox.com/optical.htm >> ______________________________________________ >> Associate Professor Guy Cox, MA, DPhil(Oxon) >> Electron Microscope Unit, Madsen Building F09, >> University of Sydney, NSW 2006 >> ______________________________________________ >> Phone +61 2 9351 3176 Fax +61 2 9351 7682 >> Mobile 0413 281 861 >> ______________________________________________ >> http://www.guycox.net >> >> >> > > ---------------------------------------- > Alberto Diaspro > Head, Nanophysics Unit > Senior Scientist > The Italian Institute of Technology -IIT > Via Morego, 30 > 16163 - Genova (Italy) > phone: +39 010 71781503 > mobile: +393666719968 > fax: +39 010 720321 > http://www.iit.it > [hidden email] > > Professor of Applied Physics > Department of Physics > University of Genova > Via Dodecaneso, 33 > 16146 Genova - Italy > tel. +39 010 353 6426 > fax. +39 010 314218 > http://www.lambs.it > [hidden email] > ------------------------------------------------------- |
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Hi Alby, Guy, Renato and the list,
Please allow us to "enjoy" a weekend holiday before getting back with information to answer some of your questions regarding Yokogawa spinning disk. I wish everybody to have a nice holiday, too！ Cheers, Mizuho *********************************************************** Mizuho Shimizu International Sales Team BIO Group B&A Center Measurement Business Headquarters Yokogawa Electric Corporation 2-3 Hokuyodai, Kanazawa-shi, Ishikawa 920-0177 JAPAN Phone: +81-76-258-7028 Fax: +81-76-258-7029 <http://www.yokogawa.com/scanner/index.htm> *********************************************************** ________________________________________ 差出人: Confocal Microscopy List [[hidden email]] は Renato A. Mortara [[hidden email]] の代理 送信日時: 2009年11月15日 5:46 宛先: [hidden email] 件名: Re: Yokogawa lenses Alby and Guy, sure enough heating with UV is a concern. I did wish someone from Yokogawa read this list and gave a conclusive answer whether any non UV-compatible element in the lightpath could be customized to be UV friendly. Grazie and Ciao, Renato Renato A. Mortara Disciplina de Parasitologia UNIFESP Escola Paulista de Medicina R. Botucatu, 862 6o andar 04023-062 S?o Paulo SP Brasil Quoting Alberto Diaspro <[hidden email]>: > It is possible that they also have to take into account the possible > heating due to intense UV > ciao > Alby > > > > > ISTITUTO ITALIANO > DI TECNOLOGIA > > Prof. Alberto Diaspro > Scientific Head > Nanophysics > Via Morego, 30 16163 Genova > Tel: +39-010.71.781.503 > Fax +39-010-72.03.21 > Mobile +39-3666719968 > www.iit.it > > > On Nov 14, 2009, at 12:55 PM, Guy Cox wrote: > >> OK, from another commercial person (who does not want to be named) >> I've now heard that in fact the Yokogawa microlenses are in fact >> silica (as I had suspected). But the snag is that there is another >> optic in the system which does not pass UV. This is a bit more >> promising, since replacing the microlenses is not an option but >> replacing a transfer lens shouldn't be difficult. Someone from >> Yokogawa must surely be reading this list. One assumes, also, that >> they would be interested in extending the application range of >> their system. So, come on, Yokogawa, please give us the real story! >> >> Guy >> >> >> Optical Imaging Techniques in Cell Biology >> by Guy Cox CRC Press / Taylor & Francis >> http://www.guycox.com/optical.htm >> ______________________________________________ >> Associate Professor Guy Cox, MA, DPhil(Oxon) >> Electron Microscope Unit, Madsen Building F09, >> University of Sydney, NSW 2006 >> ______________________________________________ >> Phone +61 2 9351 3176 Fax +61 2 9351 7682 >> Mobile 0413 281 861 >> ______________________________________________ >> http://www.guycox.net >> >> >> > > ---------------------------------------- > Alberto Diaspro > Head, Nanophysics Unit > Senior Scientist > The Italian Institute of Technology -IIT > Via Morego, 30 > 16163 - Genova (Italy) > phone: +39 010 71781503 > mobile: +393666719968 > fax: +39 010 720321 > http://www.iit.it > [hidden email] > > Professor of Applied Physics > Department of Physics > University of Genova > Via Dodecaneso, 33 > 16146 Genova - Italy > tel. +39 010 353 6426 > fax. +39 010 314218 > http://www.lambs.it > [hidden email] > ------------------------------------------------------- |
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In reply to this post by Renato A. Mortara
I am intersted in a precise answer...in case. Good luck.
Alby On Nov 14, 2009, at 9:46 PM, Renato A. Mortara wrote: > Alby and Guy, > > sure enough heating with UV is a concern. > > I did wish someone from Yokogawa read this list and gave a conclusive answer whether any non UV-compatible element in the lightpath could be customized to be > UV friendly. > > Grazie and Ciao, > > Renato > > > Renato A. Mortara > Disciplina de Parasitologia > UNIFESP Escola Paulista de Medicina > R. Botucatu, 862 6o andar > 04023-062 > S?o Paulo SP > Brasil > > > Quoting Alberto Diaspro <[hidden email]>: > >> It is possible that they also have to take into account the possible >> heating due to intense UV >> ciao >> Alby >> >> >> >> >> ISTITUTO ITALIANO >> DI TECNOLOGIA >> >> Prof. Alberto Diaspro >> Scientific Head >> Nanophysics >> Via Morego, 30 16163 Genova >> Tel: +39-010.71.781.503 >> Fax +39-010-72.03.21 >> Mobile +39-3666719968 >> www.iit.it >> >> >> On Nov 14, 2009, at 12:55 PM, Guy Cox wrote: >> >>> OK, from another commercial person (who does not want to be named) I've now heard that in fact the Yokogawa microlenses are in fact silica (as I had suspected). But the snag is that there is another optic in the system which does not pass UV. This is a bit more promising, since replacing the microlenses is not an option but replacing a transfer lens shouldn't be difficult. Someone from Yokogawa must surely be reading this list. One assumes, also, that they would be interested in extending the application range of their system. So, come on, Yokogawa, please give us the real story! >>> >>> Guy >>> >>> >>> Optical Imaging Techniques in Cell Biology >>> by Guy Cox CRC Press / Taylor & Francis >>> http://www.guycox.com/optical.htm >>> ______________________________________________ >>> Associate Professor Guy Cox, MA, DPhil(Oxon) >>> Electron Microscope Unit, Madsen Building F09, >>> University of Sydney, NSW 2006 >>> ______________________________________________ >>> Phone +61 2 9351 3176 Fax +61 2 9351 7682 >>> Mobile 0413 281 861 >>> ______________________________________________ >>> http://www.guycox.net >>> >>> >>> >> >> ---------------------------------------- >> Alberto Diaspro >> Head, Nanophysics Unit >> Senior Scientist >> The Italian Institute of Technology -IIT >> Via Morego, 30 >> 16163 - Genova (Italy) >> phone: +39 010 71781503 >> mobile: +393666719968 >> fax: +39 010 720321 >> http://www.iit.it >> [hidden email] >> >> Professor of Applied Physics >> Department of Physics >> University of Genova >> Via Dodecaneso, 33 >> 16146 Genova - Italy >> tel. +39 010 353 6426 >> fax. +39 010 314218 >> http://www.lambs.it >> [hidden email] >> ------------------------------------------------------- ---------------------------------------- Alberto Diaspro Head, Nanophysics Unit Senior Scientist The Italian Institute of Technology -IIT Via Morego, 30 16163 - Genova (Italy) phone: +39 010 71781503 mobile: +393666719968 fax: +39 010 720321 http://www.iit.it [hidden email] Professor of Applied Physics Department of Physics University of Genova Via Dodecaneso, 33 16146 Genova - Italy tel. +39 010 353 6426 fax. +39 010 314218 http://www.lambs.it [hidden email] ------------------------------------------------------- |
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In reply to this post by Mizuho Shimizu
Mizuho
I hope you had a terrrific and relaxing weekend ! Cheers Renato -----Mensagem original----- De: Confocal Microscopy List [mailto:[hidden email]] Em nome de [hidden email] Enviada em: sábado, 14 de novembro de 2009 21:14 Para: [hidden email] Assunto: Re: Yokogawa lenses Hi Alby, Guy, Renato and the list, Please allow us to "enjoy" a weekend holiday before getting back with information to answer some of your questions regarding Yokogawa spinning disk. I wish everybody to have a nice holiday, too！ Cheers, Mizuho *********************************************************** Mizuho Shimizu International Sales Team BIO Group B&A Center Measurement Business Headquarters Yokogawa Electric Corporation 2-3 Hokuyodai, Kanazawa-shi, Ishikawa 920-0177 JAPAN Phone: +81-76-258-7028 Fax: +81-76-258-7029 <http://www.yokogawa.com/scanner/index.htm> *********************************************************** ________________________________________ 差出人: Confocal Microscopy List [[hidden email]] は Renato A. Mortara [[hidden email]] の代理 送信日�r: 2009年11月15日 5:46 宛先: [hidden email] 件名: Re: Yokogawa lenses Alby and Guy, sure enough heating with UV is a concern. I did wish someone from Yokogawa read this list and gave a conclusive answer whether any non UV-compatible element in the lightpath could be customized to be UV friendly. Grazie and Ciao, Renato Renato A. Mortara Disciplina de Parasitologia UNIFESP Escola Paulista de Medicina R. Botucatu, 862 6o andar 04023-062 S?o Paulo SP Brasil Quoting Alberto Diaspro <[hidden email]>: > It is possible that they also have to take into account the possible > heating due to intense UV ciao Alby > > > > > ISTITUTO ITALIANO > DI TECNOLOGIA > > Prof. Alberto Diaspro > Scientific Head > Nanophysics > Via Morego, 30 16163 Genova > Tel: +39-010.71.781.503 > Fax +39-010-72.03.21 > Mobile +39-3666719968 > www.iit.it > > > On Nov 14, 2009, at 12:55 PM, Guy Cox wrote: > >> OK, from another commercial person (who does not want to be named) >> I've now heard that in fact the Yokogawa microlenses are in fact >> silica (as I had suspected). But the snag is that there is another >> optic in the system which does not pass UV. This is a bit more >> promising, since replacing the microlenses is not an option but >> replacing a transfer lens shouldn't be difficult. Someone from >> Yokogawa must surely be reading this list. One assumes, also, that >> they would be interested in extending the application range of their >> system. So, come on, Yokogawa, please give us the real story! >> >> Guy >> >> >> Optical Imaging Techniques in Cell Biology >> by Guy Cox CRC Press / Taylor & Francis >> http://www.guycox.com/optical.htm >> ______________________________________________ >> Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope >> Unit, Madsen Building F09, University of Sydney, NSW 2006 >> ______________________________________________ >> Phone +61 2 9351 3176 Fax +61 2 9351 7682 >> Mobile 0413 281 861 >> ______________________________________________ >> http://www.guycox.net >> >> >> > > ---------------------------------------- > Alberto Diaspro > Head, Nanophysics Unit > Senior Scientist > The Italian Institute of Technology -IIT Via Morego, 30 > 16163 - Genova (Italy) > phone: +39 010 71781503 > mobile: +393666719968 > fax: +39 010 720321 > http://www.iit.it > [hidden email] > > Professor of Applied Physics > Department of Physics > University of Genova > Via Dodecaneso, 33 > 16146 Genova - Italy > tel. +39 010 353 6426 > fax. +39 010 314218 > http://www.lambs.it > [hidden email] > ------------------------------------------------------- |
 
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Renato A. Mortara |
DAPI images and 3D recon with 405 line and Yokogawa head
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In reply to this post by Alberto Diaspro
Good morning/evening everyone,
Hope all got a nice weekend ! From a potential customer point of view, what I would really like to see are images/results with the 405 line to label DAPI for 3D reconstruction of nuclei and other DNA-rich organelles using the Yokogawa head. I am in the process of changing a "real" UV laser (Coherent 351/363) based - old BioRad 1024 confocal and Zeiss optics (40x 1.2NA -UV and 100X 1.4 NON-UV) but from our point of view, even with the non UV-100X 1.4 we still get reasonable (publishable) images of DAPI-stained structures. Since the UV lines are quite powerful what we do is to just use 10x more DAPI and we are in business with the 100X. I would be grateful for any input yet again, especially published images of DAPI with the 405 line using the Yokogawa CSU-X1 head. Renato Renato A. Mortara Parasitology Division UNIFESP - Escola Paulista de Medicina Rua Botucatu, 862, 6th floor São Paulo, SP 04023-062 Brazil Phone: 55 11 5579-8306 Fax: 55 11 5571-1095 email: [hidden email] home page: www.ecb.epm.br/~ramortara -----Mensagem original----- De: Confocal Microscopy List [mailto:[hidden email]] Em nome de Alberto Diaspro Enviada em: domingo, 15 de novembro de 2009 14:34 Para: [hidden email] Assunto: Re: Yokogawa lenses I am intersted in a precise answer...in case. Good luck. Alby On Nov 14, 2009, at 9:46 PM, Renato A. Mortara wrote: > Alby and Guy, > > sure enough heating with UV is a concern. > > I did wish someone from Yokogawa read this list and gave a conclusive > answer whether any non UV-compatible element in the lightpath could be customized to be UV friendly. > > Grazie and Ciao, > > Renato > > > Renato A. Mortara > Disciplina de Parasitologia > UNIFESP Escola Paulista de Medicina > R. Botucatu, 862 6o andar > 04023-062 > S?o Paulo SP > Brasil > > > Quoting Alberto Diaspro <[hidden email]>: > >> It is possible that they also have to take into account the possible >> heating due to intense UV ciao Alby >> >> >> >> >> ISTITUTO ITALIANO >> DI TECNOLOGIA >> >> Prof. Alberto Diaspro >> Scientific Head >> Nanophysics >> Via Morego, 30 16163 Genova >> Tel: +39-010.71.781.503 >> Fax +39-010-72.03.21 >> Mobile +39-3666719968 >> www.iit.it >> >> >> On Nov 14, 2009, at 12:55 PM, Guy Cox wrote: >> >>> OK, from another commercial person (who does not want to be named) I've had suspected). But the snag is that there is another optic in the system which does not pass UV. This is a bit more promising, since replacing the microlenses is not an option but replacing a transfer lens shouldn't be difficult. Someone from Yokogawa must surely be reading this list. One assumes, also, that they would be interested in extending the application range of their system. So, come on, Yokogawa, please give us the real story! >>> >>> Guy >>> >>> |
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Cameron, Lisa |
Re: DAPI images and 3D recon with 405 line and Yokogawa head
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Hi Renato,
My users have been taking DAPI images on my CSU-x1 system from Andor Technologies with a 405nm laser with good results. You can see: Ganem NJ, Godinho SA, Pellman D. A Mechanism Linking Extra Centrosomes to Chromosomal Instability. Nature (2009) July 9;460:278-82. I know Neil Ganem did deconvolution to help identify merotelic kinetochores but may have ended up publishing the max intensity projections and single planes. If you want to see the raw images of the DAPI illuminated with 405nm, I can ask the author. We have many other users who also collect DAPI or Pacific Blue images with 405nm laser illumination, but are not doing deconvolution. Please feel free to contact me for more information. - Lisa --------------------------------------- Lisa Cameron, Ph.D. Director of Confocal and Light Microscopy Dana Farber Cancer Institute 44 Binney St.; JF 621 Boston, MA 02115 Office phone: 617-582-8824 Fax: 617-582-8750 [hidden email] http://microscopy.dfci.harvard.edu -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Renato Mortara Sent: Monday, November 16, 2009 5:35 AM To: [hidden email] Subject: [CONFOCALMICROSCOPY] DAPI images and 3D recon with 405 line and Yokogawa head Good morning/evening everyone, Hope all got a nice weekend ! From a potential customer point of view, what I would really like to see are images/results with the 405 line to label DAPI for 3D reconstruction of nuclei and other DNA-rich organelles using the Yokogawa head. I am in the process of changing a "real" UV laser (Coherent 351/363) based - old BioRad 1024 confocal and Zeiss optics (40x 1.2NA -UV and 100X 1.4 NON-UV) but from our point of view, even with the non UV-100X 1.4 we still get reasonable (publishable) images of DAPI-stained structures. Since the UV lines are quite powerful what we do is to just use 10x more DAPI and we are in business with the 100X. I would be grateful for any input yet again, especially published images of DAPI with the 405 line using the Yokogawa CSU-X1 head. Renato Renato A. Mortara Parasitology Division UNIFESP - Escola Paulista de Medicina Rua Botucatu, 862, 6th floor São Paulo, SP 04023-062 Brazil Phone: 55 11 5579-8306 Fax: 55 11 5571-1095 email: [hidden email] home page: www.ecb.epm.br/~ramortara The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. |
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In reply to this post by Renato A. Mortara
Dear Renato, and the list.
Yes, I enjoyed gorgeous autumn weather over the weekend! While I am not in a position to officially represent Yokogawa, as Renato has mentioned already, official comment from Yokogawa, if requested, will be to ask to use the CSU spinning disk confocal system within 400nm-700nm wavelength range for best performance with a good reason. As Lisa kindly advised, it is quite OK to use the CSUX1 system at 405 nm. So, if your are talking about 405nm, it is within our specification range. However, if you are thinking about using 351/363 nm, as is well understood, imaging at UV range below 400nm could be very difficult, which is not unique to the imaging with the CSU system but universal to all optical and/or imaging devices. We have well confirmed the performance of the CSU confocal system including all components such as the microscope, laser and fibers, all filters, detector, etc.at 400-700 nm range. However, we have no conclusive data on the use below 400nm range, in reality. Our engineers don't think there could be any simple and easy way to optimize the CSU head for imaging at UV range, since every piece of optical parts both inside and outside of the CSU head requires optimization for UV. It may be possible to capture images below 400nm, but, we cannot guarantee good-enough light efficiency, confocality or chromatic aberration correction, and thus cannot endorse it. I am sorry for not being able to offer any quick solution but please kindly understand there is no magic in good UV imaging. Mizuho Shimizu Yokogawa Electric Corporation -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Renato Mortara Sent: Monday, November 16, 2009 7:06 PM To: [hidden email] Subject: RES: Yokogawa lenses Mizuho I hope you had a terrrific and relaxing weekend ! Cheers Renato -----Mensagem original----- De: Confocal Microscopy List [mailto:[hidden email]] Em nome de [hidden email] Enviada em: sábado, 14 de novembro de 2009 21:14 Para: [hidden email] Assunto: Re: Yokogawa lenses Hi Alby, Guy, Renato and the list, Please allow us to "enjoy" a weekend holiday before getting back with information to answer some of your questions regarding Yokogawa spinning disk. I wish everybody to have a nice holiday, too！ Cheers, Mizuho *********************************************************** Mizuho Shimizu International Sales Team BIO Group B&A Center Measurement Business Headquarters Yokogawa Electric Corporation 2-3 Hokuyodai, Kanazawa-shi, Ishikawa 920-0177 JAPAN Phone: +81-76-258-7028 Fax: +81-76-258-7029 <http://www.yokogawa.com/scanner/index.htm> *********************************************************** ________________________________________ 差出人: Confocal Microscopy List [[hidden email]] は Renato A. Mortara [[hidden email]] の代理 送信日�r: 2009年11月15日 5:46 宛先: [hidden email] 件名: Re: Yokogawa lenses Alby and Guy, sure enough heating with UV is a concern. I did wish someone from Yokogawa read this list and gave a conclusive answer whether any non UV-compatible element in the lightpath could be customized to be UV friendly. Grazie and Ciao, Renato Renato A. Mortara Disciplina de Parasitologia UNIFESP Escola Paulista de Medicina R. Botucatu, 862 6o andar 04023-062 S?o Paulo SP Brasil Quoting Alberto Diaspro <[hidden email]>: > It is possible that they also have to take into account the possible > heating due to intense UV ciao Alby > > > > > ISTITUTO ITALIANO > DI TECNOLOGIA > > Prof. Alberto Diaspro > Scientific Head > Nanophysics > Via Morego, 30 16163 Genova > Tel: +39-010.71.781.503 > Fax +39-010-72.03.21 > Mobile +39-3666719968 > www.iit.it > > > On Nov 14, 2009, at 12:55 PM, Guy Cox wrote: > >> OK, from another commercial person (who does not want to be named) >> I've now heard that in fact the Yokogawa microlenses are in fact >> silica (as I had suspected). But the snag is that there is another >> optic in the system which does not pass UV. This is a bit more >> promising, since replacing the microlenses is not an option but >> replacing a transfer lens shouldn't be difficult. Someone from >> Yokogawa must surely be reading this list. One assumes, also, that >> they would be interested in extending the application range of their >> system. So, come on, Yokogawa, please give us the real story! >> >> Guy >> >> >> Optical Imaging Techniques in Cell Biology >> by Guy Cox CRC Press / Taylor & Francis >> http://www.guycox.com/optical.htm >> ______________________________________________ >> Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope >> Unit, Madsen Building F09, University of Sydney, NSW 2006 >> ______________________________________________ >> Phone +61 2 9351 3176 Fax +61 2 9351 7682 >> Mobile 0413 281 861 >> ______________________________________________ >> http://www.guycox.net >> >> >> > > ---------------------------------------- > Alberto Diaspro > Head, Nanophysics Unit > Senior Scientist > The Italian Institute of Technology -IIT Via Morego, 30 > 16163 - Genova (Italy) > phone: +39 010 71781503 > mobile: +393666719968 > fax: +39 010 720321 > http://www.iit.it > [hidden email] > > Professor of Applied Physics > Department of Physics > University of Genova > Via Dodecaneso, 33 > 16146 Genova - Italy > tel. +39 010 353 6426 > fax. +39 010 314218 > http://www.lambs.it > [hidden email] > ------------------------------------------------------- |
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Dear Mizuho
Thanks for the input anyway. Best, Renato -----Mensagem original----- De: Confocal Microscopy List [mailto:[hidden email]] Em nome de [hidden email] Enviada em: segunda-feira, 16 de novembro de 2009 23:20 Para: [hidden email] Assunto: Re: Yokogawa lenses Dear Renato, and the list. Yes, I enjoyed gorgeous autumn weather over the weekend! While I am not in a position to officially represent Yokogawa, as Renato has mentioned already, official comment from Yokogawa, if requested, will be to ask to use the CSU spinning disk confocal system within 400nm-700nm wavelength range for best performance with a good reason. As Lisa kindly advised, it is quite OK to use the CSUX1 system at 405 nm. So, if your are talking about 405nm, it is within our specification range. However, if you are thinking about using 351/363 nm, as is well understood, imaging at UV range below 400nm could be very difficult, which is not unique to the imaging with the CSU system but universal to all optical and/or imaging devices. We have well confirmed the performance of the CSU confocal system including all components such as the microscope, laser and fibers, all filters, detector, etc.at 400-700 nm range. However, we have no conclusive data on the use below 400nm range, in reality. Our engineers don't think there could be any simple and easy way to optimize the CSU head for imaging at UV range, since every piece of optical parts both inside and outside of the CSU head requires optimization for UV. It may be possible to capture images below 400nm, but, we cannot guarantee good-enough light efficiency, confocality or chromatic aberration correction, and thus cannot endorse it. I am sorry for not being able to offer any quick solution but please kindly understand there is no magic in good UV imaging. Mizuho Shimizu Yokogawa Electric Corporation -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Renato Mortara Sent: Monday, November 16, 2009 7:06 PM To: [hidden email] Subject: RES: Yokogawa lenses Mizuho I hope you had a terrrific and relaxing weekend ! Cheers Renato -----Mensagem original----- De: Confocal Microscopy List [mailto:[hidden email]] Em nome de [hidden email] Enviada em: sábado, 14 de novembro de 2009 21:14 Para: [hidden email] Assunto: Re: Yokogawa lenses Hi Alby, Guy, Renato and the list, Please allow us to "enjoy" a weekend holiday before getting back with information to answer some of your questions regarding Yokogawa spinning disk. I wish everybody to have a nice holiday, too！ Cheers, Mizuho *********************************************************** Mizuho Shimizu International Sales Team BIO Group B&A Center Measurement Business Headquarters Yokogawa Electric Corporation 2-3 Hokuyodai, Kanazawa-shi, Ishikawa 920-0177 JAPAN Phone: +81-76-258-7028 Fax: +81-76-258-7029 <http://www.yokogawa.com/scanner/index.htm> *********************************************************** ________________________________________ 差出人: Confocal Microscopy List [[hidden email]] は Renato A. Mortara [[hidden email]] の代理 送信日�r: 2009年11月15日 5:46 宛先: [hidden email] 件名: Re: Yokogawa lenses Alby and Guy, sure enough heating with UV is a concern. I did wish someone from Yokogawa read this list and gave a conclusive answer whether any non UV-compatible element in the lightpath could be customized to be UV friendly. Grazie and Ciao, Renato Renato A. Mortara Disciplina de Parasitologia UNIFESP Escola Paulista de Medicina R. Botucatu, 862 6o andar 04023-062 S?o Paulo SP Brasil Quoting Alberto Diaspro <[hidden email]>: > It is possible that they also have to take into account the possible > heating due to intense UV ciao Alby > > > > > ISTITUTO ITALIANO > DI TECNOLOGIA > > Prof. Alberto Diaspro > Scientific Head > Nanophysics > Via Morego, 30 16163 Genova > Tel: +39-010.71.781.503 > Fax +39-010-72.03.21 > Mobile +39-3666719968 > www.iit.it > > > On Nov 14, 2009, at 12:55 PM, Guy Cox wrote: > >> OK, from another commercial person (who does not want to be named) >> I've now heard that in fact the Yokogawa microlenses are in fact >> silica (as I had suspected). But the snag is that there is another >> optic in the system which does not pass UV. This is a bit more >> promising, since replacing the microlenses is not an option but >> replacing a transfer lens shouldn't be difficult. Someone from >> Yokogawa must surely be reading this list. One assumes, also, that >> they would be interested in extending the application range of their >> system. So, come on, Yokogawa, please give us the real story! >> >> Guy >> >> >> Optical Imaging Techniques in Cell Biology >> by Guy Cox CRC Press / Taylor & Francis >> http://www.guycox.com/optical.htm >> ______________________________________________ >> Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope >> Unit, Madsen Building F09, University of Sydney, NSW 2006 >> ______________________________________________ >> Phone +61 2 9351 3176 Fax +61 2 9351 7682 >> Mobile 0413 281 861 >> ______________________________________________ >> http://www.guycox.net >> >> >> > > ---------------------------------------- > Alberto Diaspro > Head, Nanophysics Unit > Senior Scientist > The Italian Institute of Technology -IIT Via Morego, 30 > 16163 - Genova (Italy) > phone: +39 010 71781503 > mobile: +393666719968 > fax: +39 010 720321 > http://www.iit.it > [hidden email] > > Professor of Applied Physics > Department of Physics > University of Genova > Via Dodecaneso, 33 > 16146 Genova - Italy > tel. +39 010 353 6426 > fax. +39 010 314218 > http://www.lambs.it > [hidden email] > ------------------------------------------------------- |
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In reply to this post by Mizuho Shimizu
Well, I'm glad your weekend was good (so was mine actually, with kangaroos all around and lyre-birds playing in the garden).
But your considered opinion after all that was to give us NO information. We are scientists, we are a community. The relationship between manufacturers and users is what drives advances in the microscopy field. Please be a player in this - it really is to your advantage. What we need to know is simple - something you can tell us in an instant. What is the wavelength transmission range of your micro-lenses? For what wavelength are they chromatically corrected? What other components in your head might affect the wavelength transmission? These are not great secrets of huge commercial value. In fact, someone will certainly make the relevant measurements and post them if you don't. But in the end collaboration is what drives progress, so I hope you will change your mind and decide to become part of the community. Guy Cox Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope Unit, Madsen Building F09, University of Sydney, NSW 2006 ______________________________________________ Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of [hidden email] Sent: Tuesday, 17 November 2009 12:20 PM To: [hidden email] Subject: Re: Yokogawa lenses Dear Renato, and the list. Yes, I enjoyed gorgeous autumn weather over the weekend! While I am not in a position to officially represent Yokogawa, as Renato has mentioned already, official comment from Yokogawa, if requested, will be to ask to use the CSU spinning disk confocal system within 400nm-700nm wavelength range for best performance with a good reason. As Lisa kindly advised, it is quite OK to use the CSUX1 system at 405 nm. So, if your are talking about 405nm, it is within our specification range. However, if you are thinking about using 351/363 nm, as is well understood, imaging at UV range below 400nm could be very difficult, which is not unique to the imaging with the CSU system but universal to all optical and/or imaging devices. We have well confirmed the performance of the CSU confocal system including all components such as the microscope, laser and fibers, all filters, detector, etc.at 400-700 nm range. However, we have no conclusive data on the use below 400nm range, in reality. Our engineers don't think there could be any simple and easy way to optimize the CSU head for imaging at UV range, since every piece of optical parts both inside and outside of the CSU head requires optimization for UV. It may be possible to capture images below 400nm, but, we cannot guarantee good-enough light efficiency, confocality or chromatic aberration correction, and thus cannot endorse it. I am sorry for not being able to offer any quick solution but please kindly understand there is no magic in good UV imaging. Mizuho Shimizu Yokogawa Electric Corporation -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Renato Mortara Sent: Monday, November 16, 2009 7:06 PM To: [hidden email] Subject: RES: Yokogawa lenses Mizuho I hope you had a terrrific and relaxing weekend ! Cheers Renato -----Mensagem original----- De: Confocal Microscopy List [mailto:[hidden email]] Em nome de [hidden email] Enviada em: sábado, 14 de novembro de 2009 21:14 Para: [hidden email] Assunto: Re: Yokogawa lenses Hi Alby, Guy, Renato and the list, Please allow us to "enjoy" a weekend holiday before getting back with information to answer some of your questions regarding Yokogawa spinning disk. I wish everybody to have a nice holiday, too！ Cheers, Mizuho *********************************************************** Mizuho Shimizu International Sales Team BIO Group B&A Center Measurement Business Headquarters Yokogawa Electric Corporation 2-3 Hokuyodai, Kanazawa-shi, Ishikawa 920-0177 JAPAN Phone: +81-76-258-7028 Fax: +81-76-258-7029 <http://www.yokogawa.com/scanner/index.htm> *********************************************************** ________________________________________ 差出人: Confocal Microscopy List [[hidden email]] は Renato A. Mortara [[hidden email]] の代理 送信日�r: 2009年11月15日 5:46 宛先: [hidden email] 件名: Re: Yokogawa lenses Alby and Guy, sure enough heating with UV is a concern. I did wish someone from Yokogawa read this list and gave a conclusive answer whether any non UV-compatible element in the lightpath could be customized to be UV friendly. Grazie and Ciao, Renato Renato A. Mortara Disciplina de Parasitologia UNIFESP Escola Paulista de Medicina R. Botucatu, 862 6o andar 04023-062 S?o Paulo SP Brasil Quoting Alberto Diaspro <[hidden email]>: > It is possible that they also have to take into account the possible > heating due to intense UV ciao Alby > > > > > ISTITUTO ITALIANO > DI TECNOLOGIA > > Prof. Alberto Diaspro > Scientific Head > Nanophysics > Via Morego, 30 16163 Genova > Tel: +39-010.71.781.503 > Fax +39-010-72.03.21 > Mobile +39-3666719968 > www.iit.it > > > On Nov 14, 2009, at 12:55 PM, Guy Cox wrote: > >> OK, from another commercial person (who does not want to be named) >> I've now heard that in fact the Yokogawa microlenses are in fact >> silica (as I had suspected). But the snag is that there is another >> optic in the system which does not pass UV. This is a bit more >> promising, since replacing the microlenses is not an option but >> replacing a transfer lens shouldn't be difficult. Someone from >> Yokogawa must surely be reading this list. One assumes, also, that >> they would be interested in extending the application range of their >> system. So, come on, Yokogawa, please give us the real story! >> >> Guy >> >> >> Optical Imaging Techniques in Cell Biology >> by Guy Cox CRC Press / Taylor & Francis >> http://www.guycox.com/optical.htm >> ______________________________________________ >> Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope >> Unit, Madsen Building F09, University of Sydney, NSW 2006 >> ______________________________________________ >> Phone +61 2 9351 3176 Fax +61 2 9351 7682 >> Mobile 0413 281 861 >> ______________________________________________ >> http://www.guycox.net >> >> >> > > ---------------------------------------- > Alberto Diaspro > Head, Nanophysics Unit > Senior Scientist > The Italian Institute of Technology -IIT Via Morego, 30 > 16163 - Genova (Italy) > phone: +39 010 71781503 > mobile: +393666719968 > fax: +39 010 720321 > http://www.iit.it > [hidden email] > > Professor of Applied Physics > Department of Physics > University of Genova > Via Dodecaneso, 33 > 16146 Genova - Italy > tel. +39 010 353 6426 > fax. +39 010 314218 > http://www.lambs.it > [hidden email] > ------------------------------------------------------- No virus found in this incoming message. Checked by AVG - www.avg.com Version: 9.0.707 / Virus Database: 270.14.61/2497 - Release Date: 11/17/09 06:53:00 |
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I agree with Guy, there is much to be accomplished, the companies should stopped treating scientist as just end users and we should stop treating them as just vendors. Both parties will definitely benefit if there is real dialogue.
Best, Neeraj. Neeraj V. Gohad, Ph.D. Postdoctoral Fellow Okeanos Research Group Department of Biological Sciences 132 Long Hall Clemson University Please note my new email address: [hidden email] -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Guy Cox Sent: Tuesday, November 17, 2009 6:18 AM To: [hidden email] Subject: Re: Yokogawa lenses Well, I'm glad your weekend was good (so was mine actually, with kangaroos all around and lyre-birds playing in the garden). But your considered opinion after all that was to give us NO information. We are scientists, we are a community. The relationship between manufacturers and users is what drives advances in the microscopy field. Please be a player in this - it really is to your advantage. What we need to know is simple - something you can tell us in an instant. What is the wavelength transmission range of your micro-lenses? For what wavelength are they chromatically corrected? What other components in your head might affect the wavelength transmission? These are not great secrets of huge commercial value. In fact, someone will certainly make the relevant measurements and post them if you don't. But in the end collaboration is what drives progress, so I hope you will change your mind and decide to become part of the community. Guy Cox Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope Unit, Madsen Building F09, University of Sydney, NSW 2006 ______________________________________________ Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of [hidden email] Sent: Tuesday, 17 November 2009 12:20 PM To: [hidden email] Subject: Re: Yokogawa lenses Dear Renato, and the list. Yes, I enjoyed gorgeous autumn weather over the weekend! While I am not in a position to officially represent Yokogawa, as Renato has mentioned already, official comment from Yokogawa, if requested, will be to ask to use the CSU spinning disk confocal system within 400nm-700nm wavelength range for best performance with a good reason. As Lisa kindly advised, it is quite OK to use the CSUX1 system at 405 nm. So, if your are talking about 405nm, it is within our specification range. However, if you are thinking about using 351/363 nm, as is well understood, imaging at UV range below 400nm could be very difficult, which is not unique to the imaging with the CSU system but universal to all optical and/or imaging devices. We have well confirmed the performance of the CSU confocal system including all components such as the microscope, laser and fibers, all filters, detector, etc.at 400-700 nm range. However, we have no conclusive data on the use below 400nm range, in reality. Our engineers don't think there could be any simple and easy way to optimize the CSU head for imaging at UV range, since every piece of optical parts both inside and outside of the CSU head requires optimization for UV. It may be possible to capture images below 400nm, but, we cannot guarantee good-enough light efficiency, confocality or chromatic aberration correction, and thus cannot endorse it. I am sorry for not being able to offer any quick solution but please kindly understand there is no magic in good UV imaging. Mizuho Shimizu Yokogawa Electric Corporation -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Renato Mortara Sent: Monday, November 16, 2009 7:06 PM To: [hidden email] Subject: RES: Yokogawa lenses Mizuho I hope you had a terrrific and relaxing weekend ! Cheers Renato -----Mensagem original----- De: Confocal Microscopy List [mailto:[hidden email]] Em nome de [hidden email] Enviada em: sábado, 14 de novembro de 2009 21:14 Para: [hidden email] Assunto: Re: Yokogawa lenses Hi Alby, Guy, Renato and the list, Please allow us to "enjoy" a weekend holiday before getting back with information to answer some of your questions regarding Yokogawa spinning disk. I wish everybody to have a nice holiday, too！ Cheers, Mizuho *********************************************************** Mizuho Shimizu International Sales Team BIO Group B&A Center Measurement Business Headquarters Yokogawa Electric Corporation 2-3 Hokuyodai, Kanazawa-shi, Ishikawa 920-0177 JAPAN Phone: +81-76-258-7028 Fax: +81-76-258-7029 <http://www.yokogawa.com/scanner/index.htm> *********************************************************** ________________________________________ 差出人: Confocal Microscopy List [[hidden email]] は Renato A. Mortara [[hidden email]] の代理 送信日�r: 2009年11月15日 5:46 宛先: [hidden email] 件名: Re: Yokogawa lenses Alby and Guy, sure enough heating with UV is a concern. I did wish someone from Yokogawa read this list and gave a conclusive answer whether any non UV-compatible element in the lightpath could be customized to be UV friendly. Grazie and Ciao, Renato Renato A. Mortara Disciplina de Parasitologia UNIFESP Escola Paulista de Medicina R. Botucatu, 862 6o andar 04023-062 S?o Paulo SP Brasil Quoting Alberto Diaspro <[hidden email]>: > It is possible that they also have to take into account the possible > heating due to intense UV ciao Alby > > > > > ISTITUTO ITALIANO > DI TECNOLOGIA > > Prof. Alberto Diaspro > Scientific Head > Nanophysics > Via Morego, 30 16163 Genova > Tel: +39-010.71.781.503 > Fax +39-010-72.03.21 > Mobile +39-3666719968 > www.iit.it > > > On Nov 14, 2009, at 12:55 PM, Guy Cox wrote: > >> OK, from another commercial person (who does not want to be named) >> I've now heard that in fact the Yokogawa microlenses are in fact >> silica (as I had suspected). But the snag is that there is another >> optic in the system which does not pass UV. This is a bit more >> promising, since replacing the microlenses is not an option but >> replacing a transfer lens shouldn't be difficult. Someone from >> Yokogawa must surely be reading this list. One assumes, also, that >> they would be interested in extending the application range of their >> system. So, come on, Yokogawa, please give us the real story! >> >> Guy >> >> >> Optical Imaging Techniques in Cell Biology >> by Guy Cox CRC Press / Taylor & Francis >> http://www.guycox.com/optical.htm >> ______________________________________________ >> Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope >> Unit, Madsen Building F09, University of Sydney, NSW 2006 >> ______________________________________________ >> Phone +61 2 9351 3176 Fax +61 2 9351 7682 >> Mobile 0413 281 861 >> ______________________________________________ >> http://www.guycox.net >> >> >> > > ---------------------------------------- > Alberto Diaspro > Head, Nanophysics Unit > Senior Scientist > The Italian Institute of Technology -IIT Via Morego, 30 > 16163 - Genova (Italy) > phone: +39 010 71781503 > mobile: +393666719968 > fax: +39 010 720321 > http://www.iit.it > [hidden email] > > Professor of Applied Physics > Department of Physics > University of Genova > Via Dodecaneso, 33 > 16146 Genova - Italy > tel. +39 010 353 6426 > fax. +39 010 314218 > http://www.lambs.it > [hidden email] > ------------------------------------------------------- No virus found in this incoming message. Checked by AVG - www.avg.com Version: 9.0.707 / Virus Database: 270.14.61/2497 - Release Date: 11/17/09 06:53:00 |
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Part of the "problem" here might be in way the question is being asked. In
my experience, the "end users" should take the initiative to experiment and try new applications of existing equipment. Thereby we can empirically test these ideas on real world experimental applications. However, to properly execute this, we need to know certain things such as if UV light (eg 300-400nm) will damage the system. The question of if it will work or not is up to us to determine (ultimately by peer review). The proper question to Yokogawa in this case is will we damage the system in doing so? Clearly, the greater the exchange of information the better for all involved. But in practical terms, there is never a 100% free flow of info and I doubt there will be in the very near future. These are my two cents (Canadian, so ±1.8 US)... Paul Paul S. Maddox, PhD Assistant Professor Institute for Research in Immunology and Cancer Dept of Pathology and Cell Biol, U. de Montreal P.O. Box 6128, Station Centre-Ville Montréal QC H3C 3J7 CANADA Courier: 2900, boulevard Édouard-Montpetit Pavillon Marcelle-Coutu, Quai 20 Montreal QC H3T 1J4 CANADA [hidden email] Ph: 514-343-7894 Fax: 514-343-6843 On 17/11/09 11:17 AM, "Neeraj Gohad" <[hidden email]> wrote: > I agree with Guy, there is much to be accomplished, the companies should > stopped treating scientist as just end users and we should stop treating them > as just vendors. Both parties will definitely benefit if there is real > dialogue. > > Best, > > Neeraj. > > > Neeraj V. Gohad, Ph.D. > Postdoctoral Fellow > Okeanos Research Group > Department of Biological Sciences > 132 Long Hall > Clemson University > > Please note my new email address: [hidden email] > > > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On > Behalf Of Guy Cox > Sent: Tuesday, November 17, 2009 6:18 AM > To: [hidden email] > Subject: Re: Yokogawa lenses > > Well, I'm glad your weekend was good (so was mine actually, with kangaroos all > around and lyre-birds playing in the garden). > > But your considered opinion after all that was to give us NO information. We > are scientists, we are a community. The relationship between manufacturers > and users is what drives advances in the microscopy field. Please be a player > in this - it really is to your advantage. What we need to know is simple - > something you can tell us in an instant. What is the wavelength transmission > range of your micro-lenses? For what wavelength are they chromatically > corrected? What other components in your head might affect the wavelength > transmission? > > These are not great secrets of huge commercial value. In fact, someone will > certainly make the relevant measurements and post them if you don't. But in > the end collaboration is what drives progress, so I hope you will change your > mind and decide to become part of the community. > > > > Guy Cox > > > > Optical Imaging Techniques in Cell Biology > by Guy Cox CRC Press / Taylor & Francis > http://www.guycox.com/optical.htm > ______________________________________________ > Associate Professor Guy Cox, MA, DPhil(Oxon) > Electron Microscope Unit, Madsen Building F09, > University of Sydney, NSW 2006 > ______________________________________________ > Phone +61 2 9351 3176 Fax +61 2 9351 7682 > Mobile 0413 281 861 > ______________________________________________ > http://www.guycox.net > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On > Behalf Of [hidden email] > Sent: Tuesday, 17 November 2009 12:20 PM > To: [hidden email] > Subject: Re: Yokogawa lenses > > Dear Renato, and the list. > > Yes, I enjoyed gorgeous autumn weather over the weekend! > > > While I am not in a position to officially represent Yokogawa, as Renato has > mentioned already, official comment from Yokogawa, if requested, will be to > ask to use the CSU spinning disk confocal system within 400nm-700nm wavelength > range for best performance with a good reason. > > As Lisa kindly advised, it is quite OK to use the CSUX1 system at 405 nm. > So, if your are talking about 405nm, it is within our specification range. > > However, if you are thinking about using 351/363 nm, as is well understood, > imaging at UV range below 400nm could be very difficult, which is not unique > to the imaging with the CSU system but universal to all optical and/or imaging > devices. > We have well confirmed the performance of the CSU confocal system including > all components such as the microscope, laser and fibers, all filters, > detector, etc.at 400-700 nm range. > However, we have no conclusive data on the use below 400nm range, in reality. > > Our engineers don't think there could be any simple and easy way to optimize > the CSU head for imaging at UV range, since every piece of optical parts both > inside and outside of the CSU head requires optimization for UV. > It may be possible to capture images below 400nm, but, we cannot guarantee > good-enough light efficiency, confocality or chromatic aberration correction, > and thus cannot endorse it. > > I am sorry for not being able to offer any quick solution but please kindly > understand there is no magic in good UV imaging. > > > Mizuho Shimizu > > Yokogawa Electric Corporation > > > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On > Behalf Of Renato Mortara > Sent: Monday, November 16, 2009 7:06 PM > To: [hidden email] > Subject: RES: Yokogawa lenses > > Mizuho > I hope you had a terrrific and relaxing weekend ! > > Cheers > > Renato > > -----Mensagem original----- > De: Confocal Microscopy List [mailto:[hidden email]] Em nome > de [hidden email] Enviada em: sábado, 14 de novembro de 2009 > 21:14 > Para: [hidden email] > Assunto: Re: Yokogawa lenses > > Hi Alby, Guy, Renato and the list, > > Please allow us to "enjoy" a weekend holiday before getting back with > information to answer some of your questions regarding Yokogawa spinning disk. > > I wish everybody to have a nice holiday, too！ > > Cheers, > > Mizuho > > *********************************************************** > Mizuho Shimizu > International Sales Team > BIO Group > B&A Center > Measurement Business Headquarters > Yokogawa Electric Corporation > 2-3 Hokuyodai, Kanazawa-shi, Ishikawa > 920-0177 JAPAN > Phone: +81-76-258-7028 Fax: +81-76-258-7029 > <http://www.yokogawa.com/scanner/index.htm> > *********************************************************** > ________________________________________ > 差出人: Confocal Microscopy List [[hidden email]] は > Renato A. Mortara [[hidden email]] の代理 > 送信日時: 2009年11月15日 5:46 > 宛先: [hidden email] > 件名: Re: Yokogawa lenses > > Alby and Guy, > > sure enough heating with UV is a concern. > > I did wish someone from Yokogawa read this list and gave a conclusive answer > whether any non UV-compatible element in the lightpath could be customized to > be UV friendly. > > Grazie and Ciao, > > Renato > > > Renato A. Mortara > Disciplina de Parasitologia > UNIFESP Escola Paulista de Medicina > R. Botucatu, 862 6o andar > 04023-062 > S?o Paulo SP > Brasil > > > Quoting Alberto Diaspro <[hidden email]>: > >> It is possible that they also have to take into account the possible >> heating due to intense UV ciao Alby >> >> >> >> >> ISTITUTO ITALIANO >> DI TECNOLOGIA >> >> Prof. Alberto Diaspro >> Scientific Head >> Nanophysics >> Via Morego, 30 16163 Genova >> Tel: +39-010.71.781.503 >> Fax +39-010-72.03.21 >> Mobile +39-3666719968 >> www.iit.it >> >> >> On Nov 14, 2009, at 12:55 PM, Guy Cox wrote: >> >>> OK, from another commercial person (who does not want to be named) >>> I've now heard that in fact the Yokogawa microlenses are in fact >>> silica (as I had suspected). But the snag is that there is another >>> optic in the system which does not pass UV. This is a bit more >>> promising, since replacing the microlenses is not an option but >>> replacing a transfer lens shouldn't be difficult. Someone from >>> Yokogawa must surely be reading this list. One assumes, also, that >>> they would be interested in extending the application range of their >>> system. So, come on, Yokogawa, please give us the real story! >>> >>> Guy >>> >>> >>> Optical Imaging Techniques in Cell Biology >>> by Guy Cox CRC Press / Taylor & Francis >>> http://www.guycox.com/optical.htm >>> ______________________________________________ >>> Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope >>> Unit, Madsen Building F09, University of Sydney, NSW 2006 >>> ______________________________________________ >>> Phone +61 2 9351 3176 Fax +61 2 9351 7682 >>> Mobile 0413 281 861 >>> ______________________________________________ >>> http://www.guycox.net >>> >>> >>> >> >> ---------------------------------------- >> Alberto Diaspro >> Head, Nanophysics Unit >> Senior Scientist >> The Italian Institute of Technology -IIT Via Morego, 30 >> 16163 - Genova (Italy) >> phone: +39 010 71781503 >> mobile: +393666719968 >> fax: +39 010 720321 >> http://www.iit.it >> [hidden email] >> >> Professor of Applied Physics >> Department of Physics >> University of Genova >> Via Dodecaneso, 33 >> 16146 Genova - Italy >> tel. +39 010 353 6426 >> fax. +39 010 314218 >> http://www.lambs.it >> [hidden email] >> ------------------------------------------------------- > > No virus found in this incoming message. > Checked by AVG - www.avg.com > Version: 9.0.707 / Virus Database: 270.14.61/2497 - Release Date: 11/17/09 > 06:53:00 |
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What is great in this list is that all the information flows along opinions and ideas. I agree with Guy as well as Paul. Unfortunately, I do not have enough $$ to play around with the systems,fibers,etc, just to try to implement an application... - my 2 cents in Brazilian Reais, ± $0.10
Best Renato -----Mensagem original----- De: Confocal Microscopy List [mailto:[hidden email]] Em nome de Paul Maddox Enviada em: terça-feira, 17 de novembro de 2009 14:54 Para: [hidden email] Assunto: Re: Yokogawa lenses Part of the "problem" here might be in way the question is being asked. In my experience, the "end users" should take the initiative to experiment and try new applications of existing equipment. Thereby we can empirically test these ideas on real world experimental applications. However, to properly execute this, we need to know certain things such as if UV light (eg 300-400nm) will damage the system. The question of if it will work or not is up to us to determine (ultimately by peer review). The proper question to Yokogawa in this case is will we damage the system in doing so? Clearly, the greater the exchange of information the better for all involved. But in practical terms, there is never a 100% free flow of info and I doubt there will be in the very near future. These are my two cents (Canadian, so ±1.8 US)... Paul Paul S. Maddox, PhD Assistant Professor Institute for Research in Immunology and Cancer Dept of Pathology and Cell Biol, U. de Montreal P.O. Box 6128, Station Centre-Ville Montréal QC H3C 3J7 CANADA Courier: 2900, boulevard Édouard-Montpetit Pavillon Marcelle-Coutu, Quai 20 Montreal QC H3T 1J4 CANADA [hidden email] Ph: 514-343-7894 Fax: 514-343-6843 On 17/11/09 11:17 AM, "Neeraj Gohad" <[hidden email]> wrote: > I agree with Guy, there is much to be accomplished, the companies should > stopped treating scientist as just end users and we should stop treating them > as just vendors. Both parties will definitely benefit if there is real > dialogue. > > Best, > > Neeraj. > > > Neeraj V. Gohad, Ph.D. > Postdoctoral Fellow > Okeanos Research Group > Department of Biological Sciences > 132 Long Hall > Clemson University > > Please note my new email address: [hidden email] > > > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On > Behalf Of Guy Cox > Sent: Tuesday, November 17, 2009 6:18 AM > To: [hidden email] > Subject: Re: Yokogawa lenses > > Well, I'm glad your weekend was good (so was mine actually, with kangaroos all > around and lyre-birds playing in the garden). > > But your considered opinion after all that was to give us NO information. We > are scientists, we are a community. The relationship between manufacturers > and users is what drives advances in the microscopy field. Please be a player > in this - it really is to your advantage. What we need to know is simple - > something you can tell us in an instant. What is the wavelength transmission > range of your micro-lenses? For what wavelength are they chromatically > corrected? What other components in your head might affect the wavelength > transmission? > > These are not great secrets of huge commercial value. In fact, someone will > certainly make the relevant measurements and post them if you don't. But in > the end collaboration is what drives progress, so I hope you will change your > mind and decide to become part of the community. > > > > Guy Cox > > > > Optical Imaging Techniques in Cell Biology > by Guy Cox CRC Press / Taylor & Francis > http://www.guycox.com/optical.htm > ______________________________________________ > Associate Professor Guy Cox, MA, DPhil(Oxon) > Electron Microscope Unit, Madsen Building F09, > University of Sydney, NSW 2006 > ______________________________________________ > Phone +61 2 9351 3176 Fax +61 2 9351 7682 > Mobile 0413 281 861 > ______________________________________________ > http://www.guycox.net > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On > Behalf Of [hidden email] > Sent: Tuesday, 17 November 2009 12:20 PM > To: [hidden email] > Subject: Re: Yokogawa lenses > > Dear Renato, and the list. > > Yes, I enjoyed gorgeous autumn weather over the weekend! > > > While I am not in a position to officially represent Yokogawa, as Renato has > mentioned already, official comment from Yokogawa, if requested, will be to > ask to use the CSU spinning disk confocal system within 400nm-700nm wavelength > range for best performance with a good reason. > > As Lisa kindly advised, it is quite OK to use the CSUX1 system at 405 nm. > So, if your are talking about 405nm, it is within our specification range. > > However, if you are thinking about using 351/363 nm, as is well understood, > imaging at UV range below 400nm could be very difficult, which is not unique > to the imaging with the CSU system but universal to all optical and/or imaging > devices. > We have well confirmed the performance of the CSU confocal system including > all components such as the microscope, laser and fibers, all filters, > detector, etc.at 400-700 nm range. > However, we have no conclusive data on the use below 400nm range, in reality. > > Our engineers don't think there could be any simple and easy way to optimize > the CSU head for imaging at UV range, since every piece of optical parts both > inside and outside of the CSU head requires optimization for UV. > It may be possible to capture images below 400nm, but, we cannot guarantee > good-enough light efficiency, confocality or chromatic aberration correction, > and thus cannot endorse it. > > I am sorry for not being able to offer any quick solution but please kindly > understand there is no magic in good UV imaging. > > > Mizuho Shimizu > > Yokogawa Electric Corporation > > > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On > Behalf Of Renato Mortara > Sent: Monday, November 16, 2009 7:06 PM > To: [hidden email] > Subject: RES: Yokogawa lenses > > Mizuho > I hope you had a terrrific and relaxing weekend ! > > Cheers > > Renato > > -----Mensagem original----- > De: Confocal Microscopy List [mailto:[hidden email]] Em nome > de [hidden email] Enviada em: sábado, 14 de novembro de 2009 > 21:14 > Para: [hidden email] > Assunto: Re: Yokogawa lenses > > Hi Alby, Guy, Renato and the list, > > Please allow us to "enjoy" a weekend holiday before getting back with > information to answer some of your questions regarding Yokogawa spinning disk. > > I wish everybody to have a nice holiday, too！ > > Cheers, > > Mizuho > > *********************************************************** > Mizuho Shimizu > International Sales Team > BIO Group > B&A Center > Measurement Business Headquarters > Yokogawa Electric Corporation > 2-3 Hokuyodai, Kanazawa-shi, Ishikawa > 920-0177 JAPAN > Phone: +81-76-258-7028 Fax: +81-76-258-7029 > <http://www.yokogawa.com/scanner/index.htm> > *********************************************************** > ________________________________________ > 差出人: Confocal Microscopy List [[hidden email]] は > Renato A. Mortara [[hidden email]] の代理 > 送信日時: 2009年11月15日 5:46 > 宛先: [hidden email] > 件名: Re: Yokogawa lenses > > Alby and Guy, > > sure enough heating with UV is a concern. > > I did wish someone from Yokogawa read this list and gave a conclusive answer > whether any non UV-compatible element in the lightpath could be customized to > be UV friendly. > > Grazie and Ciao, > > Renato > > > Renato A. Mortara > Disciplina de Parasitologia > UNIFESP Escola Paulista de Medicina > R. Botucatu, 862 6o andar > 04023-062 > S?o Paulo SP > Brasil > > > Quoting Alberto Diaspro <[hidden email]>: > >> It is possible that they also have to take into account the possible >> heating due to intense UV ciao Alby >> >> >> >> >> ISTITUTO ITALIANO >> DI TECNOLOGIA >> >> Prof. Alberto Diaspro >> Scientific Head >> Nanophysics >> Via Morego, 30 16163 Genova >> Tel: +39-010.71.781.503 >> Fax +39-010-72.03.21 >> Mobile +39-3666719968 >> www.iit.it >> >> >> On Nov 14, 2009, at 12:55 PM, Guy Cox wrote: >> >>> OK, from another commercial person (who does not want to be named) >>> I've now heard that in fact the Yokogawa microlenses are in fact >>> silica (as I had suspected). But the snag is that there is another >>> optic in the system which does not pass UV. This is a bit more >>> promising, since replacing the microlenses is not an option but >>> replacing a transfer lens shouldn't be difficult. Someone from >>> Yokogawa must surely be reading this list. One assumes, also, that >>> they would be interested in extending the application range of their >>> system. So, come on, Yokogawa, please give us the real story! >>> >>> Guy >>> >>> >>> Optical Imaging Techniques in Cell Biology >>> by Guy Cox CRC Press / Taylor & Francis >>> http://www.guycox.com/optical.htm >>> ______________________________________________ >>> Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope >>> Unit, Madsen Building F09, University of Sydney, NSW 2006 >>> ______________________________________________ >>> Phone +61 2 9351 3176 Fax +61 2 9351 7682 >>> Mobile 0413 281 861 >>> ______________________________________________ >>> http://www.guycox.net >>> >>> >>> >> >> ---------------------------------------- >> Alberto Diaspro >> Head, Nanophysics Unit >> Senior Scientist >> The Italian Institute of Technology -IIT Via Morego, 30 >> 16163 - Genova (Italy) >> phone: +39 010 71781503 >> mobile: +393666719968 >> fax: +39 010 720321 >> http://www.iit.it >> [hidden email] >> >> Professor of Applied Physics >> Department of Physics >> University of Genova >> Via Dodecaneso, 33 >> 16146 Genova - Italy >> tel. +39 010 353 6426 >> fax. +39 010 314218 >> http://www.lambs.it >> [hidden email] >> ------------------------------------------------------- > > No virus found in this incoming message. > Checked by AVG - www.avg.com > Version: 9.0.707 / Virus Database: 270.14.61/2497 - Release Date: 11/17/09 > 06:53:00 |
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******** commercial interest *************
Hi Renato and Confocal List: As I stated in a previous post, we will be investigating the potential to operate the CSU at 375nm shortly. We expect the laser in about 2 weeks. As has been mentioned by another member, the potential to damage optics must always be considered. We do not believe that damage due to heating will be a factor since the heat is distributed over a fairly large area of the spinning disk. In fact if there is an issue it will be with the pinhole disk where the power densities are much higher. Damage due to UV is a greater concern but can be assessed. Many higher quality lenslet arrays are made from fused silica. Transmission tests will enable us to determine if either of the disks are a polymer or both are fused silica. If both disks are silica they are probably not UV grade fused silica, but since we are not going to hard UV the standard grade will almost certainly show no degradation at 375nm. And by the way, single mode fibers that are rated to work at 375nm are made from the same fused silica as fibers for longer wavelengths. The power densities in the fibers are orders of magnitude higher than what will be hitting the disks and so degradation will be evident in the fiber long before the disks. This is something we are going to be assessing more carefully moving forward and should not be considered a guarantee of performance at this time. And on a slightly different note, we have been very successful modifying the CSU to use lasers above 700nm, with emission wavelengths approaching 800nm. We have given the modifications a commercial name, BOREALIS. We are very excited about this development and our partner Quorum Technologies (also a commercial interest) has successfully installed two such systems with great results. Just to say that moving beyond the standard 400nm - 700nm range is possible. Best Regards, Richard Renato Mortara wrote: > What is great in this list is that all the information flows along opinions and ideas. I agree with Guy as well as Paul. Unfortunately, I do not have enough $$ to play around with the systems,fibers,etc, just to try to implement an application... - my 2 cents in Brazilian Reais, ± $0.10 > > Best > > Renato > > -----Mensagem original----- > De: Confocal Microscopy List [mailto:[hidden email]] Em nome de Paul Maddox > Enviada em: terça-feira, 17 de novembro de 2009 14:54 > Para: [hidden email] > Assunto: Re: Yokogawa lenses > > Part of the "problem" here might be in way the question is being asked. In my experience, the "end users" should take the initiative to experiment and try new applications of existing equipment. Thereby we can empirically test these ideas on real world experimental applications. However, to properly execute this, we need to know certain things such as if UV light (eg > 300-400nm) will damage the system. The question of if it will work or not is up to us to determine (ultimately by peer review). The proper question to Yokogawa in this case is will we damage the system in doing so? > > Clearly, the greater the exchange of information the better for all involved. But in practical terms, there is never a 100% free flow of info and I doubt there will be in the very near future. > > These are my two cents (Canadian, so ±1.8 US)... > > Paul > > Paul S. Maddox, PhD > Assistant Professor > Institute for Research in Immunology and Cancer Dept of Pathology and Cell Biol, U. de Montreal P.O. Box 6128, Station Centre-Ville Montréal QC H3C 3J7 CANADA > > Courier: > 2900, boulevard Édouard-Montpetit > Pavillon Marcelle-Coutu, Quai 20 > Montreal QC H3T 1J4 > CANADA > > [hidden email] > Ph: 514-343-7894 > Fax: 514-343-6843 > > > > > On 17/11/09 11:17 AM, "Neeraj Gohad" <[hidden email]> wrote: > > >> I agree with Guy, there is much to be accomplished, the companies should >> stopped treating scientist as just end users and we should stop treating them >> as just vendors. Both parties will definitely benefit if there is real >> dialogue. >> >> Best, >> >> Neeraj. >> >> >> Neeraj V. Gohad, Ph.D. >> Postdoctoral Fellow >> Okeanos Research Group >> Department of Biological Sciences >> 132 Long Hall >> Clemson University >> >> Please note my new email address: [hidden email] >> >> >> >> >> -----Original Message----- >> From: Confocal Microscopy List [mailto:[hidden email]] On >> Behalf Of Guy Cox >> Sent: Tuesday, November 17, 2009 6:18 AM >> To: [hidden email] >> Subject: Re: Yokogawa lenses >> >> Well, I'm glad your weekend was good (so was mine actually, with kangaroos all >> around and lyre-birds playing in the garden). >> >> But your considered opinion after all that was to give us NO information. We >> are scientists, we are a community. The relationship between manufacturers >> and users is what drives advances in the microscopy field. Please be a player >> in this - it really is to your advantage. What we need to know is simple - >> something you can tell us in an instant. What is the wavelength transmission >> range of your micro-lenses? For what wavelength are they chromatically >> corrected? What other components in your head might affect the wavelength >> transmission? >> >> These are not great secrets of huge commercial value. In fact, someone will >> certainly make the relevant measurements and post them if you don't. But in >> the end collaboration is what drives progress, so I hope you will change your >> mind and decide to become part of the community. >> >> >> >> Guy Cox >> >> >> >> Optical Imaging Techniques in Cell Biology >> by Guy Cox CRC Press / Taylor & Francis >> http://www.guycox.com/optical.htm >> ______________________________________________ >> Associate Professor Guy Cox, MA, DPhil(Oxon) >> Electron Microscope Unit, Madsen Building F09, >> University of Sydney, NSW 2006 >> ______________________________________________ >> Phone +61 2 9351 3176 Fax +61 2 9351 7682 >> Mobile 0413 281 861 >> ______________________________________________ >> http://www.guycox.net >> -----Original Message----- >> From: Confocal Microscopy List [mailto:[hidden email]] On >> Behalf Of [hidden email] >> Sent: Tuesday, 17 November 2009 12:20 PM >> To: [hidden email] >> Subject: Re: Yokogawa lenses >> >> Dear Renato, and the list. >> >> Yes, I enjoyed gorgeous autumn weather over the weekend! >> >> >> While I am not in a position to officially represent Yokogawa, as Renato has >> mentioned already, official comment from Yokogawa, if requested, will be to >> ask to use the CSU spinning disk confocal system within 400nm-700nm wavelength >> range for best performance with a good reason. >> >> As Lisa kindly advised, it is quite OK to use the CSUX1 system at 405 nm. >> So, if your are talking about 405nm, it is within our specification range. >> >> However, if you are thinking about using 351/363 nm, as is well understood, >> imaging at UV range below 400nm could be very difficult, which is not unique >> to the imaging with the CSU system but universal to all optical and/or imaging >> devices. >> We have well confirmed the performance of the CSU confocal system including >> all components such as the microscope, laser and fibers, all filters, >> detector, etc.at 400-700 nm range. >> However, we have no conclusive data on the use below 400nm range, in reality. >> >> Our engineers don't think there could be any simple and easy way to optimize >> the CSU head for imaging at UV range, since every piece of optical parts both >> inside and outside of the CSU head requires optimization for UV. >> It may be possible to capture images below 400nm, but, we cannot guarantee >> good-enough light efficiency, confocality or chromatic aberration correction, >> and thus cannot endorse it. >> >> I am sorry for not being able to offer any quick solution but please kindly >> understand there is no magic in good UV imaging. >> >> >> Mizuho Shimizu >> >> Yokogawa Electric Corporation >> >> >> >> >> -----Original Message----- >> From: Confocal Microscopy List [mailto:[hidden email]] On >> Behalf Of Renato Mortara >> Sent: Monday, November 16, 2009 7:06 PM >> To: [hidden email] >> Subject: RES: Yokogawa lenses >> >> Mizuho >> I hope you had a terrrific and relaxing weekend ! >> >> Cheers >> >> Renato >> >> -----Mensagem original----- >> De: Confocal Microscopy List [mailto:[hidden email]] Em nome >> de [hidden email] Enviada em: sábado, 14 de novembro de 2009 >> 21:14 >> Para: [hidden email] >> Assunto: Re: Yokogawa lenses >> >> Hi Alby, Guy, Renato and the list, >> >> Please allow us to "enjoy" a weekend holiday before getting back with >> information to answer some of your questions regarding Yokogawa spinning disk. >> >> I wish everybody to have a nice holiday, too！ >> >> Cheers, >> >> Mizuho >> >> *********************************************************** >> Mizuho Shimizu >> International Sales Team >> BIO Group >> B&A Center >> Measurement Business Headquarters >> Yokogawa Electric Corporation >> 2-3 Hokuyodai, Kanazawa-shi, Ishikawa >> 920-0177 JAPAN >> Phone: +81-76-258-7028 Fax: +81-76-258-7029 >> <http://www.yokogawa.com/scanner/index.htm> >> *********************************************************** >> ________________________________________ >> 差出人: Confocal Microscopy List [[hidden email]] は >> Renato A. Mortara [[hidden email]] の代理 >> 送信日時: 2009年11月15日 5:46 >> 宛先: [hidden email] >> 件名: Re: Yokogawa lenses >> >> Alby and Guy, >> >> sure enough heating with UV is a concern. >> >> I did wish someone from Yokogawa read this list and gave a conclusive answer >> whether any non UV-compatible element in the lightpath could be customized to >> be UV friendly. >> >> Grazie and Ciao, >> >> Renato >> >> >> Renato A. Mortara >> Disciplina de Parasitologia >> UNIFESP Escola Paulista de Medicina >> R. Botucatu, 862 6o andar >> 04023-062 >> S?o Paulo SP >> Brasil >> >> >> Quoting Alberto Diaspro <[hidden email]>: >> >> >>> It is possible that they also have to take into account the possible >>> heating due to intense UV ciao Alby >>> >>> >>> >>> >>> ISTITUTO ITALIANO >>> DI TECNOLOGIA >>> >>> Prof. Alberto Diaspro >>> Scientific Head >>> Nanophysics >>> Via Morego, 30 16163 Genova >>> Tel: +39-010.71.781.503 >>> Fax +39-010-72.03.21 >>> Mobile +39-3666719968 >>> www.iit.it >>> >>> >>> On Nov 14, 2009, at 12:55 PM, Guy Cox wrote: >>> >>> >>>> OK, from another commercial person (who does not want to be named) >>>> I've now heard that in fact the Yokogawa microlenses are in fact >>>> silica (as I had suspected). But the snag is that there is another >>>> optic in the system which does not pass UV. This is a bit more >>>> promising, since replacing the microlenses is not an option but >>>> replacing a transfer lens shouldn't be difficult. Someone from >>>> Yokogawa must surely be reading this list. One assumes, also, that >>>> they would be interested in extending the application range of their >>>> system. So, come on, Yokogawa, please give us the real story! >>>> >>>> Guy >>>> >>>> >>>> Optical Imaging Techniques in Cell Biology >>>> by Guy Cox CRC Press / Taylor & Francis >>>> http://www.guycox.com/optical.htm >>>> ______________________________________________ >>>> Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope >>>> Unit, Madsen Building F09, University of Sydney, NSW 2006 >>>> ______________________________________________ >>>> Phone +61 2 9351 3176 Fax +61 2 9351 7682 >>>> Mobile 0413 281 861 >>>> ______________________________________________ >>>> http://www.guycox.net >>>> >>>> >>>> >>>> >>> ---------------------------------------- >>> Alberto Diaspro >>> Head, Nanophysics Unit >>> Senior Scientist >>> The Italian Institute of Technology -IIT Via Morego, 30 >>> 16163 - Genova (Italy) >>> phone: +39 010 71781503 >>> mobile: +393666719968 >>> fax: +39 010 720321 >>> http://www.iit.it >>> [hidden email] >>> >>> Professor of Applied Physics >>> Department of Physics >>> University of Genova >>> Via Dodecaneso, 33 >>> 16146 Genova - Italy >>> tel. +39 010 353 6426 >>> fax. +39 010 314218 >>> http://www.lambs.it >>> [hidden email] >>> ------------------------------------------------------- >>> >> No virus found in this incoming message. >> Checked by AVG - www.avg.com >> Version: 9.0.707 / Virus Database: 270.14.61/2497 - Release Date: 11/17/09 >> 06:53:00 >> > > -- Richard Berman Spectral Applied Research 9078 Leslie St., Unit 11 Richmond Hill, Ontario L4B 3L8 905-326-5040 ext. 444 www.spectral.ca |
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-----Mensagem original----- De: Confocal Microscopy List [mailto:[hidden email]] Em nome de Richard Berman Enviada em: terça-feira, 17 de novembro de 2009 20:32 Para: [hidden email] Assunto: Re: RES: Yokogawa lenses ******** commercial interest ************* Hi Renato and Confocal List: As I stated in a previous post, we will be investigating the potential to operate the CSU at 375nm shortly. We expect the laser in about 2 weeks. As has been mentioned by another member, the potential to damage optics must always be considered. We do not believe that damage due to heating will be a factor since the heat is distributed over a fairly large area of the spinning disk. In fact if there is an issue it will be with the pinhole disk where the power densities are much higher. Damage due to UV is a greater concern but can be assessed. Many higher quality lenslet arrays are made from fused silica. Transmission tests will enable us to determine if either of the disks are a polymer or both are fused silica. If both disks are silica they are probably not UV grade fused silica, but since we are not going to hard UV the standard grade will almost certainly show no degradation at 375nm. And by the way, single mode fibers that are rated to work at 375nm are made from the same fused silica as fibers for longer wavelengths. The power densities in the fibers are orders of magnitude higher than what will be hitting the disks and so degradation will be evident in the fiber long before the disks. This is something we are going to be assessing more carefully moving forward and should not be considered a guarantee of performance at this time. And on a slightly different note, we have been very successful modifying the CSU to use lasers above 700nm, with emission wavelengths approaching 800nm. We have given the modifications a commercial name, BOREALIS. We are very excited about this development and our partner Quorum Technologies (also a commercial interest) has successfully installed two such systems with great results. Just to say that moving beyond the standard 400nm - 700nm range is possible. Best Regards, Richard Renato Mortara wrote: > What is great in this list is that all the information flows along > opinions and ideas. I agree with Guy as well as Paul. Unfortunately, I > do not have enough $$ to play around with the systems,fibers,etc, just > to try to implement an application... - my 2 cents in Brazilian Reais, > ± $0.10 > > Best > > Renato > > -----Mensagem original----- > De: Confocal Microscopy List [mailto:[hidden email]] > Em nome de Paul Maddox Enviada em: terça-feira, 17 de novembro de 2009 > 14:54 > Para: [hidden email] > Assunto: Re: Yokogawa lenses > > Part of the "problem" here might be in way the question is being > asked. In my experience, the "end users" should take the initiative > to experiment and try new applications of existing equipment. Thereby > we can empirically test these ideas on real world experimental > applications. However, to properly execute this, we need to know > certain things such as if UV light (eg > 300-400nm) will damage the system. The question of if it will work or not is up to us to determine (ultimately by peer review). The proper question to Yokogawa in this case is will we damage the system in doing so? > > Clearly, the greater the exchange of information the better for all involved. But in practical terms, there is never a 100% free flow of info and I doubt there will be in the very near future. > > These are my two cents (Canadian, so ±1.8 US)... > > Paul > > Paul S. Maddox, PhD > Assistant Professor > Institute for Research in Immunology and Cancer Dept of Pathology and > Cell Biol, U. de Montreal P.O. Box 6128, Station Centre-Ville Montréal > QC H3C 3J7 CANADA > > Courier: > 2900, boulevard Édouard-Montpetit > Pavillon Marcelle-Coutu, Quai 20 > Montreal QC H3T 1J4 > CANADA > > [hidden email] > Ph: 514-343-7894 > Fax: 514-343-6843 > > > > > On 17/11/09 11:17 AM, "Neeraj Gohad" <[hidden email]> wrote: > > >> I agree with Guy, there is much to be accomplished, the companies >> should stopped treating scientist as just end users and we should >> stop treating them as just vendors. Both parties will definitely >> benefit if there is real dialogue. >> >> Best, >> >> Neeraj. >> >> >> Neeraj V. Gohad, Ph.D. >> Postdoctoral Fellow >> Okeanos Research Group >> Department of Biological Sciences >> 132 Long Hall >> Clemson University >> >> Please note my new email address: [hidden email] >> >> >> >> >> -----Original Message----- >> From: Confocal Microscopy List >> [mailto:[hidden email]] On Behalf Of Guy Cox >> Sent: Tuesday, November 17, 2009 6:18 AM >> To: [hidden email] >> Subject: Re: Yokogawa lenses >> >> Well, I'm glad your weekend was good (so was mine actually, with >> kangaroos all around and lyre-birds playing in the garden). >> >> But your considered opinion after all that was to give us NO >> information. We are scientists, we are a community. The >> relationship between manufacturers and users is what drives advances in the microscopy field. Please be a player >> in this - it really is to your advantage. What we need to know is simple - >> something you can tell us in an instant. What is the wavelength >> transmission range of your micro-lenses? For what wavelength are >> they chromatically corrected? What other components in your head >> might affect the wavelength transmission? >> >> These are not great secrets of huge commercial value. In fact, >> someone will certainly make the relevant measurements and post them >> if you don't. But in the end collaboration is what drives progress, >> so I hope you will change your mind and decide to become part of the community. >> >> >> >> Guy Cox >> >> >> >> Optical Imaging Techniques in Cell Biology >> by Guy Cox CRC Press / Taylor & Francis >> http://www.guycox.com/optical.htm >> ______________________________________________ >> Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope >> Unit, Madsen Building F09, University of Sydney, NSW 2006 >> ______________________________________________ >> Phone +61 2 9351 3176 Fax +61 2 9351 7682 >> Mobile 0413 281 861 >> ______________________________________________ >> http://www.guycox.net >> -----Original Message----- >> From: Confocal Microscopy List >> [mailto:[hidden email]] On Behalf Of >> [hidden email] >> Sent: Tuesday, 17 November 2009 12:20 PM >> To: [hidden email] >> Subject: Re: Yokogawa lenses >> >> Dear Renato, and the list. >> >> Yes, I enjoyed gorgeous autumn weather over the weekend! >> >> >> While I am not in a position to officially represent Yokogawa, as >> Renato has mentioned already, official comment from Yokogawa, if >> requested, will be to ask to use the CSU spinning disk confocal >> system within 400nm-700nm wavelength range for best performance with a good reason. >> >> As Lisa kindly advised, it is quite OK to use the CSUX1 system at 405 nm. >> So, if your are talking about 405nm, it is within our specification range. >> >> However, if you are thinking about using 351/363 nm, as is well >> understood, imaging at UV range below 400nm could be very difficult, >> which is not unique to the imaging with the CSU system but universal >> to all optical and/or imaging devices. >> We have well confirmed the performance of the CSU confocal system >> including all components such as the microscope, laser and fibers, >> all filters, detector, etc.at 400-700 nm range. >> However, we have no conclusive data on the use below 400nm range, in reality. >> >> Our engineers don't think there could be any simple and easy way to >> optimize the CSU head for imaging at UV range, since every piece of >> optical parts both inside and outside of the CSU head requires optimization for UV. >> It may be possible to capture images below 400nm, but, we cannot >> guarantee good-enough light efficiency, confocality or chromatic >> aberration correction, and thus cannot endorse it. >> >> I am sorry for not being able to offer any quick solution but please >> kindly understand there is no magic in good UV imaging. >> >> >> Mizuho Shimizu >> >> Yokogawa Electric Corporation >> >> >> >> >> -----Original Message----- >> From: Confocal Microscopy List >> [mailto:[hidden email]] On Behalf Of Renato Mortara >> Sent: Monday, November 16, 2009 7:06 PM >> To: [hidden email] >> Subject: RES: Yokogawa lenses >> >> Mizuho >> I hope you had a terrrific and relaxing weekend ! >> >> Cheers >> >> Renato >> >> -----Mensagem original----- >> De: Confocal Microscopy List >> [mailto:[hidden email]] Em nome de >> [hidden email] Enviada em: sábado, 14 de novembro de >> 2009 >> 21:14 >> Para: [hidden email] >> Assunto: Re: Yokogawa lenses >> >> Hi Alby, Guy, Renato and the list, >> >> Please allow us to "enjoy" a weekend holiday before getting back with >> information to answer some of your questions regarding Yokogawa spinning disk. >> >> I wish everybody to have a nice holiday, too！ >> >> Cheers, >> >> Mizuho >> >> *********************************************************** >> Mizuho Shimizu >> International Sales Team >> BIO Group >> B&A Center >> Measurement Business Headquarters >> Yokogawa Electric Corporation >> 2-3 Hokuyodai, Kanazawa-shi, Ishikawa >> 920-0177 JAPAN >> Phone: +81-76-258-7028 Fax: +81-76-258-7029 >> <http://www.yokogawa.com/scanner/index.htm> >> *********************************************************** >> ________________________________________ >> 差出人: Confocal Microscopy List [[hidden email]] は >> Renato A. Mortara [[hidden email]] の代理 >> 送信日時: 2009年11月15日 5:46 >> 宛先: [hidden email] >> 件名: Re: Yokogawa lenses >> >> Alby and Guy, >> >> sure enough heating with UV is a concern. >> >> I did wish someone from Yokogawa read this list and gave a conclusive >> answer whether any non UV-compatible element in the lightpath could >> be customized to be UV friendly. >> >> Grazie and Ciao, >> >> Renato >> >> >> Renato A. Mortara >> Disciplina de Parasitologia >> UNIFESP Escola Paulista de Medicina >> R. Botucatu, 862 6o andar >> 04023-062 >> S?o Paulo SP >> Brasil >> >> >> Quoting Alberto Diaspro <[hidden email]>: >> >> >>> It is possible that they also have to take into account the possible >>> heating due to intense UV ciao Alby >>> >>> >>> >>> >>> ISTITUTO ITALIANO >>> DI TECNOLOGIA >>> >>> Prof. Alberto Diaspro >>> Scientific Head >>> Nanophysics >>> Via Morego, 30 16163 Genova >>> Tel: +39-010.71.781.503 >>> Fax +39-010-72.03.21 >>> Mobile +39-3666719968 >>> www.iit.it >>> >>> >>> On Nov 14, 2009, at 12:55 PM, Guy Cox wrote: >>> >>> >>>> OK, from another commercial person (who does not want to be named) >>>> I've now heard that in fact the Yokogawa microlenses are in fact >>>> silica (as I had suspected). But the snag is that there is another >>>> optic in the system which does not pass UV. This is a bit more >>>> promising, since replacing the microlenses is not an option but >>>> replacing a transfer lens shouldn't be difficult. Someone from >>>> Yokogawa must surely be reading this list. One assumes, also, that >>>> they would be interested in extending the application range of >>>> their system. So, come on, Yokogawa, please give us the real story! >>>> >>>> Guy >>>> >>>> >>>> Optical Imaging Techniques in Cell Biology >>>> by Guy Cox CRC Press / Taylor & Francis >>>> http://www.guycox.com/optical.htm >>>> ______________________________________________ >>>> Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope >>>> Unit, Madsen Building F09, University of Sydney, NSW 2006 >>>> ______________________________________________ >>>> Phone +61 2 9351 3176 Fax +61 2 9351 7682 >>>> Mobile 0413 281 861 >>>> ______________________________________________ >>>> http://www.guycox.net >>>> >>>> >>>> >>>> >>> ---------------------------------------- >>> Alberto Diaspro >>> Head, Nanophysics Unit >>> Senior Scientist >>> The Italian Institute of Technology -IIT Via Morego, 30 >>> 16163 - Genova (Italy) >>> phone: +39 010 71781503 >>> mobile: +393666719968 >>> fax: +39 010 720321 >>> http://www.iit.it >>> [hidden email] >>> >>> Professor of Applied Physics >>> Department of Physics >>> University of Genova >>> Via Dodecaneso, 33 >>> 16146 Genova - Italy >>> tel. +39 010 353 6426 >>> fax. +39 010 314218 >>> http://www.lambs.it >>> [hidden email] >>> ------------------------------------------------------- >>> >> No virus found in this incoming message. >> Checked by AVG - www.avg.com >> Version: 9.0.707 / Virus Database: 270.14.61/2497 - Release Date: >> 11/17/09 06:53:00 >> > > -- Richard Berman Spectral Applied Research 9078 Leslie St., Unit 11 Richmond Hill, Ontario L4B 3L8 905-326-5040 ext. 444 www.spectral.ca |
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In reply to this post by Richard Berman
Hi Richard and list members,
It is great that you will be able to bench test UV lines on the Yokogawa head and fibers. Keeping my fingers crossed for a positive outcome ! Best Renato -----Mensagem original----- De: Confocal Microscopy List [mailto:[hidden email]] Em nome de Richard Berman Enviada em: terça-feira, 17 de novembro de 2009 20:32 Para: [hidden email] Assunto: Re: RES: Yokogawa lenses ******** commercial interest ************* Hi Renato and Confocal List: As I stated in a previous post, we will be investigating the potential to operate the CSU at 375nm shortly. We expect the laser in about 2 weeks. As has been mentioned by another member, the potential to damage optics must always be considered. We do not believe that damage due to heating will be a factor since the heat is distributed over a fairly large area of the spinning disk. In fact if there is an issue it will be with the pinhole disk where the power densities are much higher. Damage due to UV is a greater concern but can be assessed. Many higher quality lenslet arrays are made from fused silica. Transmission tests will enable us to determine if either of the disks are a polymer or both are fused silica. If both disks are silica they are probably not UV grade fused silica, but since we are not going to hard UV the standard grade will almost certainly show no degradation at 375nm. And by the way, single mode fibers that are rated to work at 375nm are made from the same fused silica as fibers for longer wavelengths. The power densities in the fibers are orders of magnitude higher than what will be hitting the disks and so degradation will be evident in the fiber long before the disks. This is something we are going to be assessing more carefully moving forward and should not be considered a guarantee of performance at this time. And on a slightly different note, we have been very successful modifying the CSU to use lasers above 700nm, with emission wavelengths approaching 800nm. We have given the modifications a commercial name, BOREALIS. We are very excited about this development and our partner Quorum Technologies (also a commercial interest) has successfully installed two such systems with great results. Just to say that moving beyond the standard 400nm - 700nm range is possible. Best Regards, Richard Renato Mortara wrote: > What is great in this list is that all the information flows along > opinions and ideas. I agree with Guy as well as Paul. Unfortunately, I > do not have enough $$ to play around with the systems,fibers,etc, just > to try to implement an application... - my 2 cents in Brazilian Reais, > ± $0.10 > > Best > > Renato > > -----Mensagem original----- > De: Confocal Microscopy List [mailto:[hidden email]] > Em nome de Paul Maddox Enviada em: terça-feira, 17 de novembro de 2009 > 14:54 > Para: [hidden email] > Assunto: Re: Yokogawa lenses > > Part of the "problem" here might be in way the question is being > asked. In my experience, the "end users" should take the initiative > to experiment and try new applications of existing equipment. Thereby > we can empirically test these ideas on real world experimental > applications. However, to properly execute this, we need to know > certain things such as if UV light (eg > 300-400nm) will damage the system. The question of if it will work or not is up to us to determine (ultimately by peer review). The proper question to Yokogawa in this case is will we damage the system in doing so? > > Clearly, the greater the exchange of information the better for all involved. But in practical terms, there is never a 100% free flow of info and I doubt there will be in the very near future. > > These are my two cents (Canadian, so ±1.8 US)... > > Paul > > Paul S. Maddox, PhD > Assistant Professor > Institute for Research in Immunology and Cancer Dept of Pathology and > Cell Biol, U. de Montreal P.O. Box 6128, Station Centre-Ville Montréal > QC H3C 3J7 CANADA > > Courier: > 2900, boulevard Édouard-Montpetit > Pavillon Marcelle-Coutu, Quai 20 > Montreal QC H3T 1J4 > CANADA > > [hidden email] > Ph: 514-343-7894 > Fax: 514-343-6843 > > > > > On 17/11/09 11:17 AM, "Neeraj Gohad" <[hidden email]> wrote: > > >> I agree with Guy, there is much to be accomplished, the companies >> should stopped treating scientist as just end users and we should >> stop treating them as just vendors. Both parties will definitely >> benefit if there is real dialogue. >> >> Best, >> >> Neeraj. >> >> >> Neeraj V. Gohad, Ph.D. >> Postdoctoral Fellow >> Okeanos Research Group >> Department of Biological Sciences >> 132 Long Hall >> Clemson University >> >> Please note my new email address: [hidden email] >> >> >> >> >> -----Original Message----- >> From: Confocal Microscopy List >> [mailto:[hidden email]] On Behalf Of Guy Cox >> Sent: Tuesday, November 17, 2009 6:18 AM >> To: [hidden email] >> Subject: Re: Yokogawa lenses >> >> Well, I'm glad your weekend was good (so was mine actually, with >> kangaroos all around and lyre-birds playing in the garden). >> >> But your considered opinion after all that was to give us NO >> information. We are scientists, we are a community. The >> relationship between manufacturers and users is what drives advances in the microscopy field. Please be a player >> in this - it really is to your advantage. What we need to know is simple - >> something you can tell us in an instant. What is the wavelength >> transmission range of your micro-lenses? For what wavelength are >> they chromatically corrected? What other components in your head >> might affect the wavelength transmission? >> >> These are not great secrets of huge commercial value. In fact, >> someone will certainly make the relevant measurements and post them >> if you don't. But in the end collaboration is what drives progress, >> so I hope you will change your mind and decide to become part of the community. >> >> >> >> Guy Cox >> >> >> >> Optical Imaging Techniques in Cell Biology >> by Guy Cox CRC Press / Taylor & Francis >> http://www.guycox.com/optical.htm >> ______________________________________________ >> Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope >> Unit, Madsen Building F09, University of Sydney, NSW 2006 >> ______________________________________________ >> Phone +61 2 9351 3176 Fax +61 2 9351 7682 >> Mobile 0413 281 861 >> ______________________________________________ >> http://www.guycox.net >> -----Original Message----- >> From: Confocal Microscopy List >> [mailto:[hidden email]] On Behalf Of >> [hidden email] >> Sent: Tuesday, 17 November 2009 12:20 PM >> To: [hidden email] >> Subject: Re: Yokogawa lenses >> >> Dear Renato, and the list. >> >> Yes, I enjoyed gorgeous autumn weather over the weekend! >> >> >> While I am not in a position to officially represent Yokogawa, as >> Renato has mentioned already, official comment from Yokogawa, if >> requested, will be to ask to use the CSU spinning disk confocal >> system within 400nm-700nm wavelength range for best performance with a good reason. >> >> As Lisa kindly advised, it is quite OK to use the CSUX1 system at 405 nm. >> So, if your are talking about 405nm, it is within our specification range. >> >> However, if you are thinking about using 351/363 nm, as is well >> understood, imaging at UV range below 400nm could be very difficult, >> which is not unique to the imaging with the CSU system but universal >> to all optical and/or imaging devices. >> We have well confirmed the performance of the CSU confocal system >> including all components such as the microscope, laser and fibers, >> all filters, detector, etc.at 400-700 nm range. >> However, we have no conclusive data on the use below 400nm range, in reality. >> >> Our engineers don't think there could be any simple and easy way to >> optimize the CSU head for imaging at UV range, since every piece of >> optical parts both inside and outside of the CSU head requires optimization for UV. >> It may be possible to capture images below 400nm, but, we cannot >> guarantee good-enough light efficiency, confocality or chromatic >> aberration correction, and thus cannot endorse it. >> >> I am sorry for not being able to offer any quick solution but please >> kindly understand there is no magic in good UV imaging. >> >> >> Mizuho Shimizu >> >> Yokogawa Electric Corporation >> >> >> >> >> -----Original Message----- >> From: Confocal Microscopy List >> [mailto:[hidden email]] On Behalf Of Renato Mortara >> Sent: Monday, November 16, 2009 7:06 PM >> To: [hidden email] >> Subject: RES: Yokogawa lenses >> >> Mizuho >> I hope you had a terrrific and relaxing weekend ! >> >> Cheers >> >> Renato >> >> -----Mensagem original----- >> De: Confocal Microscopy List >> [mailto:[hidden email]] Em nome de >> [hidden email] Enviada em: sábado, 14 de novembro de >> 2009 >> 21:14 >> Para: [hidden email] >> Assunto: Re: Yokogawa lenses >> >> Hi Alby, Guy, Renato and the list, >> >> Please allow us to "enjoy" a weekend holiday before getting back with >> information to answer some of your questions regarding Yokogawa spinning disk. >> >> I wish everybody to have a nice holiday, too！ >> >> Cheers, >> >> Mizuho >> >> *********************************************************** >> Mizuho Shimizu >> International Sales Team >> BIO Group >> B&A Center >> Measurement Business Headquarters >> Yokogawa Electric Corporation >> 2-3 Hokuyodai, Kanazawa-shi, Ishikawa >> 920-0177 JAPAN >> Phone: +81-76-258-7028 Fax: +81-76-258-7029 >> <http://www.yokogawa.com/scanner/index.htm> >> *********************************************************** >> ________________________________________ >> 差出人: Confocal Microscopy List [[hidden email]] は >> Renato A. Mortara [[hidden email]] の代理 >> 送信日時: 2009年11月15日 5:46 >> 宛先: [hidden email] >> 件名: Re: Yokogawa lenses >> >> Alby and Guy, >> >> sure enough heating with UV is a concern. >> >> I did wish someone from Yokogawa read this list and gave a conclusive >> answer whether any non UV-compatible element in the lightpath could >> be customized to be UV friendly. >> >> Grazie and Ciao, >> >> Renato >> >> >> Renato A. Mortara >> Disciplina de Parasitologia >> UNIFESP Escola Paulista de Medicina >> R. Botucatu, 862 6o andar >> 04023-062 >> S?o Paulo SP >> Brasil >> >> >> Quoting Alberto Diaspro <[hidden email]>: >> >> >>> It is possible that they also have to take into account the possible >>> heating due to intense UV ciao Alby >>> >>> >>> >>> >>> ISTITUTO ITALIANO >>> DI TECNOLOGIA >>> >>> Prof. Alberto Diaspro >>> Scientific Head >>> Nanophysics >>> Via Morego, 30 16163 Genova >>> Tel: +39-010.71.781.503 >>> Fax +39-010-72.03.21 >>> Mobile +39-3666719968 >>> www.iit.it >>> >>> >>> On Nov 14, 2009, at 12:55 PM, Guy Cox wrote: >>> >>> >>>> OK, from another commercial person (who does not want to be named) >>>> I've now heard that in fact the Yokogawa microlenses are in fact >>>> silica (as I had suspected). But the snag is that there is another >>>> optic in the system which does not pass UV. This is a bit more >>>> promising, since replacing the microlenses is not an option but >>>> replacing a transfer lens shouldn't be difficult. Someone from >>>> Yokogawa must surely be reading this list. One assumes, also, that >>>> they would be interested in extending the application range of >>>> their system. So, come on, Yokogawa, please give us the real story! >>>> >>>> Guy >>>> >>>> >>>> Optical Imaging Techniques in Cell Biology >>>> by Guy Cox CRC Press / Taylor & Francis >>>> http://www.guycox.com/optical.htm >>>> ______________________________________________ >>>> Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope >>>> Unit, Madsen Building F09, University of Sydney, NSW 2006 >>>> ______________________________________________ >>>> Phone +61 2 9351 3176 Fax +61 2 9351 7682 >>>> Mobile 0413 281 861 >>>> ______________________________________________ >>>> http://www.guycox.net >>>> >>>> >>>> >>>> >>> ---------------------------------------- >>> Alberto Diaspro >>> Head, Nanophysics Unit >>> Senior Scientist >>> The Italian Institute of Technology -IIT Via Morego, 30 >>> 16163 - Genova (Italy) >>> phone: +39 010 71781503 >>> mobile: +393666719968 >>> fax: +39 010 720321 >>> http://www.iit.it >>> [hidden email] >>> >>> Professor of Applied Physics >>> Department of Physics >>> University of Genova >>> Via Dodecaneso, 33 >>> 16146 Genova - Italy >>> tel. +39 010 353 6426 >>> fax. +39 010 314218 >>> http://www.lambs.it >>> [hidden email] >>> ------------------------------------------------------- >>> >> No virus found in this incoming message. >> Checked by AVG - www.avg.com >> Version: 9.0.707 / Virus Database: 270.14.61/2497 - Release Date: >> 11/17/09 06:53:00 >> > > -- Richard Berman Spectral Applied Research 9078 Leslie St., Unit 11 Richmond Hill, Ontario L4B 3L8 905-326-5040 ext. 444 www.spectral.ca |
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