Fluorescence Lifetime Imaging

[Katharina Thomsen]

Dear Lister,

In summer we will start a new project involving Fluorescence Lifetime
Imaging (FLIM). This project will necessitate an upgrade to our already
existing  microscopic set-up, especially the computer and the software.
We want to use widefield frequency domain FLIM. I was wondering, if
anyone knows or has an imaging program for frequency domain FLIM. I
could do the hardware acquisition myself, but I think I could save a lot
of time, if I didn't have to code up the calculation of the lifetime and
the imaging of the lifetime... We want to use our gain-modulated CCD
camera Hamamatsu (c4880-80) for imaging .

Thanks a lot
Katharina

[Csúcs Gábor]

Dear Katharina,

I think Intelligent Imaging Innovations has a relatively flexible FLIM
system.
No commercial Interest.

Gabor

> Dear Lister,
>
> In summer we will start a new project involving Fluorescence Lifetime
> Imaging (FLIM). This project will necessitate an upgrade to our already
> existing  microscopic set-up, especially the computer and the software.
> We want to use widefield frequency domain FLIM. I was wondering, if
> anyone knows or has an imaging program for frequency domain FLIM. I
> could do the hardware acquisition myself, but I think I could save a lot
> of time, if I didn't have to code up the calculation of the lifetime and
> the imaging of the lifetime... We want to use our gain-modulated CCD
> camera Hamamatsu (c4880-80) for imaging .
>
> Thanks a lot
> Katharina

--
Gabor Csucs
Light Microscopy Centre, ETH Zurich
Schafmattstrasse 18, HPM F16
CH-8093, Zurich, Switzerland

Web: www.lmc.ethz.ch
Phone: [phone]
Mobile: [phone]
Fax: [phone]
e-mail: [hidden email]

[Alessandro Esposito]

Dear Katharina,
     I think that all companies that provides software for FD-FLIM would
provide it only for their own system, but you may try to contact them.

Although I do not have experience with it, I guess that the only software
easily available for FDFLIM is Globals by Enrico Gratton that you will easily find
on the web.

If you preferred sofware that you can modify and you have Matlab, I could
send you a toolbox I developed during my PhD. I used to have it online, but
currently it needs some clean-up, but if you are interested we can look into it.

Cheers,

Alessandro

www.quantitative-microscopy.org
www.hutchison-mrc.cam.ac.uk

no commercial interest

[Periasamy, Ammasi (ap3t)]

Hi Katherina
I do not know your background...if you are a Physicist or experienced in lifetime estimation, then definitely you can do what you plan to do. Otherwise it will be a waste of time. There are companies like Lambert instrument sells unit which can be adoptable to any vendors wide field microscope system. You can just by the unit and attach to your existing microscope. Another suggestion is...... you can attend our FRET workshop where you have the opportunity to work with most of the commercial lifetime imaging system and probably you can get more input from the vendors. Our workshop this year is March 9-13, 2010.
Hope this helps.
 

Ammasi Periasamy, Ph.D.
Director, Keck Center for Cellular Imaging (KCCI)
Professor of Biology and Biomedical Engineering
Biology, Gilmer Hall (064), 485 McCormick Rd
University of Virginia
Charlottesville, VA 22904
Voice: 434-243-7602 (Office); 982-4869 (lab)
Fax:[phone]; Email:[hidden email]
http://www.kcci.virginia.edu
************************
10th Annual Workshop on FRET Microscopy, March 8-12, 2011
http://www.kcci.virginia.edu/workshop/workshop2011/index.php
*************************


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Katharina Thomsen
Sent: Tuesday, February 16, 2010 11:50 AM
To: [hidden email]
Subject: Fluorescence Lifetime Imaging

Dear Lister,

In summer we will start a new project involving Fluorescence Lifetime
Imaging (FLIM). This project will necessitate an upgrade to our already
existing  microscopic set-up, especially the computer and the software.
We want to use widefield frequency domain FLIM. I was wondering, if
anyone knows or has an imaging program for frequency domain FLIM. I
could do the hardware acquisition myself, but I think I could save a lot
of time, if I didn't have to code up the calculation of the lifetime and
the imaging of the lifetime... We want to use our gain-modulated CCD
camera Hamamatsu (c4880-80) for imaging .

Thanks a lot
Katharina