Fluorescent beads
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hello We are looking for very bright, monocolour (green or red), 50-100 nm fluorescent beads for some PSF test. Does anyone know which beads are brightest? Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website<https://ki.se/en/bionut/welcome-to-the-lci-facility> Follow our microscopy blog!<http://microscopykarolinska.se/> När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://ki.se/medarbetare/integritetsskyddspolicy>. Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI's processing of personal data here<https://ki.se/en/staff/data-protection-policy>. |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi! FluoSpheres are fantastic. We use them routinely, much brighter than TetraSpeck beads (maybe 100x depending on bead size). See this paper on fiducial correction using FluoSpheres: https://iopscience.iop.org/article/10.1088/1361-6463/ab0862/meta Product page: https://www.thermofisher.com/uk/en/home/references/molecular-probes-the-handbook/tables/summary-of-molecular-probes-fluospheres-fluorescent-microspheres.html Not very cheap unfortunately. Best wishes, Aleks ________________________________ From: Confocal Microscopy List <[hidden email]> on behalf of Sylvie Le Guyader <[hidden email]> Sent: 06 April 2021 14:27 To: [hidden email] <[hidden email]> Subject: Fluorescent beads ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hello We are looking for very bright, monocolour (green or red), 50-100 nm fluorescent beads for some PSF test. Does anyone know which beads are brightest? Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website<https://ki.se/en/bionut/welcome-to-the-lci-facility> Follow our microscopy blog!<http://microscopykarolinska.se/> När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://ki.se/medarbetare/integritetsskyddspolicy>. Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI's processing of personal data here<https://ki.se/en/staff/data-protection-policy>. |
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In reply to this post by Sylvie Le Guyader
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi, apart from the existing and widespread FluoSpheres, we just received and are going to test and compare the PSF of the yellow 50-90 nm beads from SpheroTech (https://www.spherotech.com/up_to_5.htm , number FP-00552-2). We'll know more on their brightness the following days. Best Orestis __________________________________ Orestis Faklaris, PhD MRI, Montpellier Ressources Imagerie CRBM-CNRS 1919 route de Mende 34293 Montpellier FRANCE phone: +33.4.34.35.95.18 www.mri.cnrs.fr -- Le 06/04/2021 à 15:27, Sylvie Le Guyader a écrit : > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hello > > We are looking for very bright, monocolour (green or red), 50-100 nm fluorescent beads for some PSF test. > Does anyone know which beads are brightest? > > Med vänlig hälsning / Best regards > > Sylvie > > @@@@@@@@@@@@@@@@@@@@@@@@ > Sylvie Le Guyader, PhD > Live Cell Imaging Facility Manager > Karolinska Institutet- Bionut Dpt > Blickagången 16, > Room 7362 (lab)/7840 (office) > 14157 Huddinge, Sweden > mobile: +46 (0) 73 733 5008 > LCI website<https://ki.se/en/bionut/welcome-to-the-lci-facility> > Follow our microscopy blog!<http://microscopykarolinska.se/> > > > > > När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://ki.se/medarbetare/integritetsskyddspolicy>. > > > Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI's processing of personal data here<https://ki.se/en/staff/data-protection-policy>. |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Can anyone recommend a source for beads made of a material other than polystyrene? I'm looking to make measurements in dibenzyl ether, which will dissolve styrene beads. Thanks so much, Drew Friedmann On Tue, Apr 6, 2021 at 2:12 PM Orestis Faklaris < [hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi, > > apart from the existing and widespread FluoSpheres, we just received and > are going to test and compare the PSF of the yellow 50-90 nm beads from > SpheroTech (https://www.spherotech.com/up_to_5.htm , number FP-00552-2). > We'll know more on their brightness the following days. > > Best > > Orestis > > > __________________________________ > Orestis Faklaris, PhD > MRI, Montpellier Ressources Imagerie > CRBM-CNRS > 1919 route de Mende > 34293 Montpellier > FRANCE > > phone: +33.4.34.35.95.18 > www.mri.cnrs.fr > -- > > Le 06/04/2021 à 15:27, Sylvie Le Guyader a écrit : > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > Post images on http://www.imgur.com and include the link in your > posting. > > ***** > > > > Hello > > > > We are looking for very bright, monocolour (green or red), 50-100 nm > fluorescent beads for some PSF test. > > Does anyone know which beads are brightest? > > > > Med vänlig hälsning / Best regards > > > > Sylvie > > > > @@@@@@@@@@@@@@@@@@@@@@@@ > > Sylvie Le Guyader, PhD > > Live Cell Imaging Facility Manager > > Karolinska Institutet- Bionut Dpt > > Blickagången 16, > > Room 7362 (lab)/7840 (office) > > 14157 Huddinge, Sweden > > mobile: +46 (0) 73 733 5008 > > LCI website<https://ki.se/en/bionut/welcome-to-the-lci-facility> > > Follow our microscopy blog!<http://microscopykarolinska.se/> > > > > > > > > > > När du skickar e-post till Karolinska Institutet (KI) innebär detta att > KI kommer att behandla dina personuppgifter. Här finns information om hur > KI behandlar personuppgifter< > https://ki.se/medarbetare/integritetsskyddspolicy>. > > > > > > Sending email to Karolinska Institutet (KI) will result in KI processing > your personal data. You can read more about KI's processing of personal > data here<https://ki.se/en/staff/data-protection-policy>. > |
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In reply to this post by Orestis Faklaris
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear Sylvie, I would contact ThermoFisher and ask if they have FluoSpheres in green and red in size 40 nm or 100 nm in storage (https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/tables/summary-of-molecular-probes-fluospheres-fluorescent-microspheres.html). Some of these beads are currently not listed on their website but believe it or not, you can still order them. I have performed some of my Ph.D. experiments with Crimson 100 nm beads that do not exist, according to their website. An entire custom-made batch costs approx. 12,000 Euro, but occasionally, labs order them custom-made, and ThermoFisher still might have some of these not listed beads in store. Best of luck. Best regards, Ulrike ____________________ Ulrike Boehm, Ph.D. Research Specialist | Advanced Imaging Center Howard Hughes Medical Institute | Janelia Research Campus 19700 Helix Drive, Ashburn, VA 20147 Email: [hidden email] Phone: 571-209-4000 x1356 -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Orestis Faklaris Sent: Tuesday, April 6, 2021 4:05 PM To: [hidden email] Subject: Re: Fluorescent beads External Email: Use Caution ***** To join, leave or search the confocal microscopy listserv, go to: https://urldefense.com/v3/__http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy__;!!Eh6p8Q!RacJT6MFQZREzM0VgS723rZx8jF4mfPN6Td8xcV3iwmDKKUhhgFxghw1jWNyP07XXoU$ Post images on https://urldefense.com/v3/__http://www.imgur.com__;!!Eh6p8Q!RacJT6MFQZREzM0VgS723rZx8jF4mfPN6Td8xcV3iwmDKKUhhgFxghw1jWNysq5N79E$ and include the link in your posting. ***** Hi, apart from the existing and widespread FluoSpheres, we just received and are going to test and compare the PSF of the yellow 50-90 nm beads from SpheroTech (https://urldefense.com/v3/__https://www.spherotech.com/up_to_5.htm__;!!Eh6p8Q!RacJT6MFQZREzM0VgS723rZx8jF4mfPN6Td8xcV3iwmDKKUhhgFxghw1jWNye1cA4tU$ , number FP-00552-2). We'll know more on their brightness the following days. Best Orestis __________________________________ Orestis Faklaris, PhD MRI, Montpellier Ressources Imagerie CRBM-CNRS 1919 route de Mende 34293 Montpellier FRANCE phone: +33.4.34.35.95.18 https://urldefense.com/v3/__http://www.mri.cnrs.fr__;!!Eh6p8Q!RacJT6MFQZREzM0VgS723rZx8jF4mfPN6Td8xcV3iwmDKKUhhgFxghw1jWNyCwDWy4s$ -- Le 06/04/2021 à 15:27, Sylvie Le Guyader a écrit : > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://urldefense.com/v3/__http://lists.umn.edu/cgi-bin/wa?A0=confoca > lmicroscopy__;!!Eh6p8Q!RacJT6MFQZREzM0VgS723rZx8jF4mfPN6Td8xcV3iwmDKKU > hhgFxghw1jWNyP07XXoU$ Post images on > https://urldefense.com/v3/__http://www.imgur.com__;!!Eh6p8Q!RacJT6MFQZREzM0VgS723rZx8jF4mfPN6Td8xcV3iwmDKKUhhgFxghw1jWNysq5N79E$ and include the link in your posting. > ***** > > Hello > > We are looking for very bright, monocolour (green or red), 50-100 nm fluorescent beads for some PSF test. > Does anyone know which beads are brightest? > > Med vänlig hälsning / Best regards > > Sylvie > > @@@@@@@@@@@@@@@@@@@@@@@@ > Sylvie Le Guyader, PhD > Live Cell Imaging Facility Manager > Karolinska Institutet- Bionut Dpt > Blickagången 16, > Room 7362 (lab)/7840 (office) > 14157 Huddinge, Sweden > mobile: +46 (0) 73 733 5008 > LCI > website<https://urldefense.com/v3/__https://ki.se/en/bionut/welcome-to > -the-lci-facility__;!!Eh6p8Q!RacJT6MFQZREzM0VgS723rZx8jF4mfPN6Td8xcV3i > wmDKKUhhgFxghw1jWNyuluB-64$ > Follow our microscopy > blog!<https://urldefense.com/v3/__http://microscopykarolinska.se/__;!! > Eh6p8Q!RacJT6MFQZREzM0VgS723rZx8jF4mfPN6Td8xcV3iwmDKKUhhgFxghw1jWNyYuc > 5jQI$ > > > > > > När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://urldefense.com/v3/__https://ki.se/medarbetare/integritetsskyddspolicy__;!!Eh6p8Q!RacJT6MFQZREzM0VgS723rZx8jF4mfPN6Td8xcV3iwmDKKUhhgFxghw1jWNyoHM2xQo$ >. > > > Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI's processing of personal data here<https://urldefense.com/v3/__https://ki.se/en/staff/data-protection-policy__;!!Eh6p8Q!RacJT6MFQZREzM0VgS723rZx8jF4mfPN6Td8xcV3iwmDKKUhhgFxghw1jWNyhiLgZEc$ >. |
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In reply to this post by Drew Friedmann
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Drew. I would go for golden beads and measure in reflected light. Best. Petro On Wed, Apr 7, 2021, 01:59 Drew Friedmann <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Can anyone recommend a source for beads made of a material other than > polystyrene? I'm looking to make measurements in dibenzyl ether, which will > dissolve styrene beads. > Thanks so much, > Drew Friedmann > > > On Tue, Apr 6, 2021 at 2:12 PM Orestis Faklaris < > [hidden email]> wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > Post images on http://www.imgur.com and include the link in your > posting. > > ***** > > > > Hi, > > > > apart from the existing and widespread FluoSpheres, we just received and > > are going to test and compare the PSF of the yellow 50-90 nm beads from > > SpheroTech (https://www.spherotech.com/up_to_5.htm , number FP-00552-2). > > We'll know more on their brightness the following days. > > > > Best > > > > Orestis > > > > > > __________________________________ > > Orestis Faklaris, PhD > > MRI, Montpellier Ressources Imagerie > > CRBM-CNRS > > 1919 route de Mende > > 34293 Montpellier > > FRANCE > > > > phone: +33.4.34.35.95.18 > > www.mri.cnrs.fr > > -- > > > > Le 06/04/2021 à 15:27, Sylvie Le Guyader a écrit : > > > ***** > > > To join, leave or search the confocal microscopy listserv, go to: > > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > > Post images on http://www.imgur.com and include the link in your > > posting. > > > ***** > > > > > > Hello > > > > > > We are looking for very bright, monocolour (green or red), 50-100 nm > > fluorescent beads for some PSF test. > > > Does anyone know which beads are brightest? > > > > > > Med vänlig hälsning / Best regards > > > > > > Sylvie > > > > > > @@@@@@@@@@@@@@@@@@@@@@@@ > > > Sylvie Le Guyader, PhD > > > Live Cell Imaging Facility Manager > > > Karolinska Institutet- Bionut Dpt > > > Blickagången 16, > > > Room 7362 (lab)/7840 (office) > > > 14157 Huddinge, Sweden > > > mobile: +46 (0) 73 733 5008 > > > LCI website<https://ki.se/en/bionut/welcome-to-the-lci-facility> > > > Follow our microscopy blog!<http://microscopykarolinska.se/> > > > > > > > > > > > > > > > När du skickar e-post till Karolinska Institutet (KI) innebär detta att > > KI kommer att behandla dina personuppgifter. Här finns information om hur > > KI behandlar personuppgifter< > > https://ki.se/medarbetare/integritetsskyddspolicy>. > > > > > > > > > Sending email to Karolinska Institutet (KI) will result in KI > processing > > your personal data. You can read more about KI's processing of personal > > data here<https://ki.se/en/staff/data-protection-policy>. > > >
Postdoctoral Fellow
Laboratory of Advanced Optical Microscopy Institute of Organic Chemistry and Biochemistry of the CAS Flemingovo nám. 2 | 166 10 Praha 6 | Czech Republic |
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In reply to this post by Drew Friedmann
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Sicastar beads (made out of silica) work in DBE: https://www.micromod.de/de/produkte-3-fluoreszent.html I don't know of any silica beads with more than one fluorophore, and would be interested to hear if someone else has found any. Best, Pablo Pablo Ariel, Ph.D. Assistant Professor Director of the Microscopy Services Laboratory Brinkhous-Bullitt Bldg B04 Department of Pathology and Laboratory Medicine University of North Carolina at Chapel Hill http://www.med.unc.edu/microscopy Tel: 919-966-2413 -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Drew Friedmann Sent: Tuesday, April 6, 2021 7:41 PM To: [hidden email] Subject: Re: Fluorescent beads ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Can anyone recommend a source for beads made of a material other than polystyrene? I'm looking to make measurements in dibenzyl ether, which will dissolve styrene beads. Thanks so much, Drew Friedmann On Tue, Apr 6, 2021 at 2:12 PM Orestis Faklaris < [hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi, > > apart from the existing and widespread FluoSpheres, we just received > and are going to test and compare the PSF of the yellow 50-90 nm beads > from SpheroTech (https://www.spherotech.com/up_to_5.htm , number FP-00552-2). > We'll know more on their brightness the following days. > > Best > > Orestis > > > __________________________________ > Orestis Faklaris, PhD > MRI, Montpellier Ressources Imagerie > CRBM-CNRS > 1919 route de Mende > 34293 Montpellier > FRANCE > > phone: +33.4.34.35.95.18 > www.mri.cnrs.fr > -- > > Le 06/04/2021 à 15:27, Sylvie Le Guyader a écrit : > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > Post images on http://www.imgur.com and include the link in your > posting. > > ***** > > > > Hello > > > > We are looking for very bright, monocolour (green or red), 50-100 nm > fluorescent beads for some PSF test. > > Does anyone know which beads are brightest? > > > > Med vänlig hälsning / Best regards > > > > Sylvie > > > > @@@@@@@@@@@@@@@@@@@@@@@@ > > Sylvie Le Guyader, PhD > > Live Cell Imaging Facility Manager > > Karolinska Institutet- Bionut Dpt > > Blickagången 16, > > Room 7362 (lab)/7840 (office) > > 14157 Huddinge, Sweden > > mobile: +46 (0) 73 733 5008 > > LCI website<https://ki.se/en/bionut/welcome-to-the-lci-facility> > > Follow our microscopy blog!<http://microscopykarolinska.se/> > > > > > > > > > > När du skickar e-post till Karolinska Institutet (KI) innebär detta > > att > KI kommer att behandla dina personuppgifter. Här finns information om > hur KI behandlar personuppgifter< > https://ki.se/medarbetare/integritetsskyddspolicy>. > > > > > > Sending email to Karolinska Institutet (KI) will result in KI > > processing > your personal data. You can read more about KI's processing of > personal data here<https://ki.se/en/staff/data-protection-policy>. > |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Thanks a lot everyone for the great input! 😊 Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website Follow our microscopy blog! -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Ariel, Pablo Sent: 07 April 2021 12:48 To: [hidden email] Subject: Re: Fluorescent beads ***** To join, leave or search the confocal microscopy listserv, go to: https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=04%7C01%7Csylvie.le.guyader%40KI.SE%7Cec99a74dcc8249c41d7d08d8f9b72f83%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637533912467096213%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=zbvuQGBGlMC07DCe0Q4HSHmcjtPKD1R9AquU8WwAadI%3D&reserved=0 Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=04%7C01%7Csylvie.le.guyader%40KI.SE%7Cec99a74dcc8249c41d7d08d8f9b72f83%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637533912467096213%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=%2FR8oxzdYPFCpHEPiX%2B035vj1xsTqLzkSW0n68%2B1d02Q%3D&reserved=0 and include the link in your posting. ***** Sicastar beads (made out of silica) work in DBE: https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.micromod.de%2Fde%2Fprodukte-3-fluoreszent.html&data=04%7C01%7Csylvie.le.guyader%40KI.SE%7Cec99a74dcc8249c41d7d08d8f9b72f83%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637533912467096213%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=1adb21xhmI3IO%2Brxx5GfYSg4f3yg0TCJD3qcM1xxif0%3D&reserved=0 I don't know of any silica beads with more than one fluorophore, and would be interested to hear if someone else has found any. Best, Pablo Pablo Ariel, Ph.D. Assistant Professor Director of the Microscopy Services Laboratory Brinkhous-Bullitt Bldg B04 Department of Pathology and Laboratory Medicine University of North Carolina at Chapel Hill https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.med.unc.edu%2Fmicroscopy&data=04%7C01%7Csylvie.le.guyader%40KI.SE%7Cec99a74dcc8249c41d7d08d8f9b72f83%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637533912467096213%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=2qnYNmkuYHFqeVcoWzXrrr1pzTMFlXW6mtQ2fQ5vcbo%3D&reserved=0 Tel: 919-966-2413 -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Drew Friedmann Sent: Tuesday, April 6, 2021 7:41 PM To: [hidden email] Subject: Re: Fluorescent beads ***** To join, leave or search the confocal microscopy listserv, go to: https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=04%7C01%7Csylvie.le.guyader%40KI.SE%7Cec99a74dcc8249c41d7d08d8f9b72f83%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637533912467096213%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=zbvuQGBGlMC07DCe0Q4HSHmcjtPKD1R9AquU8WwAadI%3D&reserved=0 Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=04%7C01%7Csylvie.le.guyader%40KI.SE%7Cec99a74dcc8249c41d7d08d8f9b72f83%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637533912467096213%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=%2FR8oxzdYPFCpHEPiX%2B035vj1xsTqLzkSW0n68%2B1d02Q%3D&reserved=0 and include the link in your posting. ***** Can anyone recommend a source for beads made of a material other than polystyrene? I'm looking to make measurements in dibenzyl ether, which will dissolve styrene beads. Thanks so much, Drew Friedmann On Tue, Apr 6, 2021 at 2:12 PM Orestis Faklaris < [hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists > .umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=04%7C01%7Cs > ylvie.le.guyader%40KI.SE%7Cec99a74dcc8249c41d7d08d8f9b72f83%7Cbff7eef1 > cf4b4f32be3da1dda043c05d%7C0%7C0%7C637533912467096213%7CUnknown%7CTWFp > bGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn > 0%3D%7C1000&sdata=zbvuQGBGlMC07DCe0Q4HSHmcjtPKD1R9AquU8WwAadI%3D&a > mp;reserved=0 Post images on > https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=04%7C01%7Csylvie.le.guyader%40KI.SE%7Cec99a74dcc8249c41d7d08d8f9b72f83%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637533912467096213%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=%2FR8oxzdYPFCpHEPiX%2B035vj1xsTqLzkSW0n68%2B1d02Q%3D&reserved=0 and include the link in your posting. > ***** > > Hi, > > apart from the existing and widespread FluoSpheres, we just received > and are going to test and compare the PSF of the yellow 50-90 nm beads > from SpheroTech (https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.spherotech.com%2Fup_to_5.htm&data=04%7C01%7Csylvie.le.guyader%40KI.SE%7Cec99a74dcc8249c41d7d08d8f9b72f83%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637533912467096213%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=btCqdmgSd7w%2BH72NXJDf7z%2BOCwY1DBcsocoe%2ByzIqPg%3D&reserved=0 , number FP-00552-2). > We'll know more on their brightness the following days. > > Best > > Orestis > > > __________________________________ > Orestis Faklaris, PhD > MRI, Montpellier Ressources Imagerie > CRBM-CNRS > 1919 route de Mende > 34293 Montpellier > FRANCE > > phone: +33.4.34.35.95.18 > https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.m > ri.cnrs.fr%2F&data=04%7C01%7Csylvie.le.guyader%40KI.SE%7Cec99a74dc > c8249c41d7d08d8f9b72f83%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C6 > 37533912467096213%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoi > V2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=NiZ0S%2FNJxqWT > tYVwqc6B9yDkFJ416vfFGrtgMas%2FqNg%3D&reserved=0 > -- > > Le 06/04/2021 à 15:27, Sylvie Le Guyader a écrit : > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flis > > ts.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=04%7C01 > > %7Csylvie.le.guyader%40KI.SE%7Cec99a74dcc8249c41d7d08d8f9b72f83%7Cbf > > f7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637533912467096213%7CUnknow > > n%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwi > > LCJXVCI6Mn0%3D%7C1000&sdata=zbvuQGBGlMC07DCe0Q4HSHmcjtPKD1R9AquU > > 8WwAadI%3D&reserved=0 Post images on > > https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww > > .imgur.com%2F&data=04%7C01%7Csylvie.le.guyader%40KI.SE%7Cec99a74 > > dcc8249c41d7d08d8f9b72f83%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0 > > %7C637533912467106206%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLC > > JQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=A4V5ak > > vZFs7PlFdACrnrU%2BTV0Smsr4q0JwY1MhCXnNs%3D&reserved=0 and > > include the link in your > posting. > > ***** > > > > Hello > > > > We are looking for very bright, monocolour (green or red), 50-100 nm > fluorescent beads for some PSF test. > > Does anyone know which beads are brightest? > > > > Med vänlig hälsning / Best regards > > > > Sylvie > > > > @@@@@@@@@@@@@@@@@@@@@@@@ > > Sylvie Le Guyader, PhD > > Live Cell Imaging Facility Manager > > Karolinska Institutet- Bionut Dpt > > Blickagången 16, > > Room 7362 (lab)/7840 (office) > > 14157 Huddinge, Sweden > > mobile: +46 (0) 73 733 5008 > > LCI > > website<https://eur01.safelinks.protection.outlook.com/?url=https%3A > > %2F%2Fki.se%2Fen%2Fbionut%2Fwelcome-to-the-lci-facility&data=04% > > 7C01%7Csylvie.le.guyader%40KI.SE%7Cec99a74dcc8249c41d7d08d8f9b72f83% > > 7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637533912467106206%7CUn > > known%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1h > > aWwiLCJXVCI6Mn0%3D%7C1000&sdata=z2cxpRkvui2ZoTiXuaMycHtDc8vbuoMy > > CagWDGRTPbk%3D&reserved=0> Follow our microscopy > > blog!<https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F > > %2Fmicroscopykarolinska.se%2F&data=04%7C01%7Csylvie.le.guyader%4 > > 0KI.SE%7Cec99a74dcc8249c41d7d08d8f9b72f83%7Cbff7eef1cf4b4f32be3da1dd > > a043c05d%7C0%7C0%7C637533912467106206%7CUnknown%7CTWFpbGZsb3d8eyJWIj > > oiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000& > > amp;sdata=x2RlQeEdI6zZ1K%2Bkizh4QQHl4%2B%2Bayr72YOVxjvD26lo%3D&r > > eserved=0> > > > > > > > > > > När du skickar e-post till Karolinska Institutet (KI) innebär detta > > att > KI kommer att behandla dina personuppgifter. Här finns information om > hur KI behandlar personuppgifter< > https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fki.se%2Fmedarbetare%2Fintegritetsskyddspolicy&data=04%7C01%7Csylvie.le.guyader%40KI.SE%7Cec99a74dcc8249c41d7d08d8f9b72f83%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637533912467106206%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=CJrCd%2FolVFGwg5FCxeUbqVToLhMTTnITBtQ3afTu2Co%3D&reserved=0>. > > > > > > Sending email to Karolinska Institutet (KI) will result in KI > > processing > your personal data. You can read more about KI's processing of > personal data here<https://ki.se/en/staff/data-protection-policy>. > |
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In reply to this post by Drew Friedmann
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Drew, you should have a look here, they have a lot of different non styrene beads coming in many sizes and with different fluorophores: https://www.micromod.de/en/produkte-3-fluorescent.html I have no commercial interest. Best Roland Dr. Roland Nitschke Albert-Ludwigs University Freiburg Life Imaging Center (LIC) Microscopy and Image Analysis Platform (MIAP) Habsburgerstr.49 79104 Freiburg Germany e-mail: [hidden email] <mailto:[hidden email]> phone:+49 761 203 2934 web LIC:https://miap.eu/miap-units/life-imaging-center-lic <https://miap.eu/miap-units/life-imaging-center-lic> web MIAP:https://miap.eu <https://miap.eu/> Am 07.04.2021 um 01:41 schrieb Drew Friedmann: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Can anyone recommend a source for beads made of a material other than > polystyrene? I'm looking to make measurements in dibenzyl ether, which will > dissolve styrene beads. > Thanks so much, > Drew Friedmann > > > On Tue, Apr 6, 2021 at 2:12 PM Orestis Faklaris < > [hidden email]> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> Post images on http://www.imgur.com and include the link in your posting. >> ***** >> >> Hi, >> >> apart from the existing and widespread FluoSpheres, we just received and >> are going to test and compare the PSF of the yellow 50-90 nm beads from >> SpheroTech (https://www.spherotech.com/up_to_5.htm , number FP-00552-2). >> We'll know more on their brightness the following days. >> >> Best >> >> Orestis >> >> >> __________________________________ >> Orestis Faklaris, PhD >> MRI, Montpellier Ressources Imagerie >> CRBM-CNRS >> 1919 route de Mende >> 34293 Montpellier >> FRANCE >> >> phone: +33.4.34.35.95.18 >> www.mri.cnrs.fr >> -- >> >> Le 06/04/2021 à 15:27, Sylvie Le Guyader a écrit : >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> Post images on http://www.imgur.com and include the link in your >> posting. >>> ***** >>> >>> Hello >>> >>> We are looking for very bright, monocolour (green or red), 50-100 nm >> fluorescent beads for some PSF test. >>> Does anyone know which beads are brightest? >>> >>> Med vänlig hälsning / Best regards >>> >>> Sylvie >>> >>> @@@@@@@@@@@@@@@@@@@@@@@@ >>> Sylvie Le Guyader, PhD >>> Live Cell Imaging Facility Manager >>> Karolinska Institutet- Bionut Dpt >>> Blickagången 16, >>> Room 7362 (lab)/7840 (office) >>> 14157 Huddinge, Sweden >>> mobile: +46 (0) 73 733 5008 >>> LCI website<https://ki.se/en/bionut/welcome-to-the-lci-facility> >>> Follow our microscopy blog!<http://microscopykarolinska.se/> >>> >>> >>> >>> >>> När du skickar e-post till Karolinska Institutet (KI) innebär detta att >> KI kommer att behandla dina personuppgifter. Här finns information om hur >> KI behandlar personuppgifter< >> https://ki.se/medarbetare/integritetsskyddspolicy>. >>> >>> Sending email to Karolinska Institutet (KI) will result in KI processing >> your personal data. You can read more about KI's processing of personal >> data here<https://ki.se/en/staff/data-protection-policy>. >> |
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In reply to this post by Drew Friedmann
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Drew, my favorite Expo Neon dry erase marker beads are quite resistant, I suspect melamine, but I'm no chemist. I haven't tried DBE, but they do not dissolve in acetone. Note that they are quite big, about 0.3 um. Best, zdenek On Tue, Apr 6, 2021 at 7:59 PM Drew Friedmann <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Can anyone recommend a source for beads made of a material other than > polystyrene? I'm looking to make measurements in dibenzyl ether, which will > dissolve styrene beads. > Thanks so much, > Drew Friedmann > > > On Tue, Apr 6, 2021 at 2:12 PM Orestis Faklaris < > [hidden email]> wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > Post images on http://www.imgur.com and include the link in your > posting. > > ***** > > > > Hi, > > > > apart from the existing and widespread FluoSpheres, we just received and > > are going to test and compare the PSF of the yellow 50-90 nm beads from > > SpheroTech (https://www.spherotech.com/up_to_5.htm , number FP-00552-2). > > We'll know more on their brightness the following days. > > > > Best > > > > Orestis > > > > > > __________________________________ > > Orestis Faklaris, PhD > > MRI, Montpellier Ressources Imagerie > > CRBM-CNRS > > 1919 route de Mende > > 34293 Montpellier > > FRANCE > > > > phone: +33.4.34.35.95.18 > > www.mri.cnrs.fr > > -- > > > > Le 06/04/2021 à 15:27, Sylvie Le Guyader a écrit : > > > ***** > > > To join, leave or search the confocal microscopy listserv, go to: > > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > > Post images on http://www.imgur.com and include the link in your > > posting. > > > ***** > > > > > > Hello > > > > > > We are looking for very bright, monocolour (green or red), 50-100 nm > > fluorescent beads for some PSF test. > > > Does anyone know which beads are brightest? > > > > > > Med vänlig hälsning / Best regards > > > > > > Sylvie > > > > > > @@@@@@@@@@@@@@@@@@@@@@@@ > > > Sylvie Le Guyader, PhD > > > Live Cell Imaging Facility Manager > > > Karolinska Institutet- Bionut Dpt > > > Blickagången 16, > > > Room 7362 (lab)/7840 (office) > > > 14157 Huddinge, Sweden > > > mobile: +46 (0) 73 733 5008 > > > LCI website<https://ki.se/en/bionut/welcome-to-the-lci-facility> > > > Follow our microscopy blog!<http://microscopykarolinska.se/> > > > > > > > > > > > > > > > När du skickar e-post till Karolinska Institutet (KI) innebär detta att > > KI kommer att behandla dina personuppgifter. Här finns information om hur > > KI behandlar personuppgifter< > > https://ki.se/medarbetare/integritetsskyddspolicy>. > > > > > > > > > Sending email to Karolinska Institutet (KI) will result in KI > processing > > your personal data. You can read more about KI's processing of personal > > data here<https://ki.se/en/staff/data-protection-policy>. > > > -- -- Zdenek Svindrych, Ph.D. Research Scientist - Microscopy Imaging Specialist Department of Biochemistry and Cell Biology Geisel School of Medicine at Dartmouth |
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