Fluorescent labelling of the antibody

[Ewa_B]

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Hello everyone,

I am looking for good kit for fluorescent labelling of the antibody. The
brighter, the better.
Did you use something like that? Do you have any suggestions? All i had
tried until now did not work well enough.

Thank you,
Ewa

--
Ewa Biela, Ph.D.
Postdoctoral Research Associate
Basic Medical Sciences
Lyn Hall, 625 Harrison Street
West Lafayette, IN 47907 - 2058
Ph [phone]
www.cyto.purdue.edu

[Martin Wessendorf-2]

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Dear Dr. Biela--

What have you tried so far?

Also, do you want to directly conjugate your primary antibody with a
fluorophore?  If not, you may get the brightest labeling by conjugating
it (or a secondary antibody) with biotin and using a fluorescently
labeled streptavidin.

Good luck!

Martin Wessendorf




On 7/8/2016 9:19 AM, Ewa Biela wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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> *****
>
> Hello everyone,
>
> I am looking for good kit for fluorescent labelling of the antibody. The
> brighter, the better.
> Did you use something like that? Do you have any suggestions? All i had
> tried until now did not work well enough.
>
> Thank you,
> Ewa
>
> --
> Ewa Biela, Ph.D.
> Postdoctoral Research Associate
> Basic Medical Sciences
> Lyn Hall, 625 Harrison Street
> West Lafayette, IN 47907 - 2058
> Ph [phone]
> www.cyto.purdue.edu

--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]

[Raghavendra Palanakar]

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Invitrogen SiteClick QD labelling kit- super bright and stable for long term imaging



  Original Message  
From: Ewa Biela
Sent: Friday, 8 July 2016 17:11
To: [hidden email]
Reply To: Confocal Microscopy List
Subject: [CONFOCALMICROSCOPY] Fluorescent labelling of the antibody

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Hello everyone,

I am looking for good kit for fluorescent labelling of the antibody. The
brighter, the better.
Did you use something like that? Do you have any suggestions? All i had
tried until now did not work well enough.

Thank you,
Ewa

--
Ewa Biela, Ph.D.
Postdoctoral Research Associate
Basic Medical Sciences
Lyn Hall, 625 Harrison Street
West Lafayette, IN 47907 - 2058
Ph [phone]
www.cyto.purdue.edu

[Joshua Zachary Rappoport]

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People seem to like this one:
https://www.thermofisher.com/order/catalog/product/A20181

Josh

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ewa Biela
Sent: Friday, July 08, 2016 9:19 AM
To: [hidden email]
Subject: Fluorescent labelling of the antibody

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Hello everyone,

I am looking for good kit for fluorescent labelling of the antibody. The brighter, the better.
Did you use something like that? Do you have any suggestions? All i had tried until now did not work well enough.

Thank you,
Ewa

--
Ewa Biela, Ph.D.
Postdoctoral Research Associate
Basic Medical Sciences
Lyn Hall, 625 Harrison Street
West Lafayette, IN 47907 - 2058
Ph [phone]
www.cyto.purdue.edu

[Ewa_B]

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Thank you all for answers.

I have been trying two different kits until now.
Zenon labelling kit from Thermofisher and Lightning Link from Innova
Biosciences. Both worked, but the signal was too weak.
I was also considering using biotin - fluorescent streptavidin, but I
wanted to ask what other users think about available products.




On 8 July 2016 at 11:19, Raghavendra Palanakar <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Invitrogen SiteClick QD labelling kit- super bright and stable for long
> term imaging
>
>
>
>   Original Message
> From: Ewa Biela
> Sent: Friday, 8 July 2016 17:11
> To: [hidden email]
> Reply To: Confocal Microscopy List
> Subject: [CONFOCALMICROSCOPY] Fluorescent labelling of the antibody
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello everyone,
>
> I am looking for good kit for fluorescent labelling of the antibody. The
> brighter, the better.
> Did you use something like that? Do you have any suggestions? All i had
> tried until now did not work well enough.
>
> Thank you,
> Ewa
>
> --
> Ewa Biela, Ph.D.
> Postdoctoral Research Associate
> Basic Medical Sciences
> Lyn Hall, 625 Harrison Street
> West Lafayette, IN 47907 - 2058
> Ph [phone]
> www.cyto.purdue.edu
>