Fluorescent microsphere recommendations

[John Oreopoulos]

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Hello list,

Can anyone recommend a source of yellow/green fluorescent microspheres (200 nm - 1 um diameter)? These would be for demonstration purposes, so the surface chemistry is not important to me. Looking for a fairly high concentration (maybe stock powder form?). Commercial vendors can reply to me directly offline.

Sincerely,

John Oreopoulos

[Cvic Innocent]

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Hey there John

Im a fan of these:
https://www.thermofisher.com/order/catalog/product/F8811?ICID=search-product

Be sure to sonicate before using them, however, for a more even
distribution onto your coverslip

Cordially,
cvic

-
CInnocent
Schermelleh Lab
Micron ABU
Oxford

On Tue, Apr 19, 2016 at 3:42 PM, John Oreopoulos <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello list,
>
> Can anyone recommend a source of yellow/green fluorescent microspheres
> (200 nm - 1 um diameter)? These would be for demonstration purposes, so the
> surface chemistry is not important to me. Looking for a fairly high
> concentration (maybe stock powder form?). Commercial vendors can reply to
> me directly offline.
>
> Sincerely,
>
> John Oreopoulos

[George McNamara]

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Hi John,

https://www.thermofisher.com/order/catalog/product/F8852


  FluoSpheres® Sulfate Microspheres, 1.0 µm, yellow-green fluorescent
  (505/515), 2% solids

Other sizes, colors, in table inside
https://tools.thermofisher.com/content/sfs/manuals/mp05000.pdf
including 0.2 um and 0.5 um options.

I also encourage having a tube of their Constellation microspheres
(potpurri of all the microsphere products -- maybe mixed all the runs
that failed manufacturing specs over the years into a catalog item?)
https://www.thermofisher.com/order/catalog/product/C14837

Back to yellow-green beads ...
https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/tables/molecular-probes-yellow-green-fluorescent-fluospheres-beads-compared-with-other-commercially-available-yellow-green-fluorescent-microspheres.html


      Table 6.5 Molecular Probes yellow-green–fluorescent FluoSpheres
      beads compared with other commercially available
      yellow-green–fluorescent microspheres.

Brand/Supplier
Size (µm)
       
Fluorescence Intensity *
       
CV for Intensity †
Molecular ProbesF8852
<https://www.thermofisher.com/order/catalog/en/US/adirect/lt?cmd=catProductDetail&entryPoint=adirect&productID=F8852&messageType=catProductDetail>
        1.02 1998 4.40%
Molecular ProbesF8853
<https://www.thermofisher.com/order/catalog/en/US/adirect/lt?cmd=catProductDetail&entryPoint=adirect&productID=F8853&messageType=catProductDetail>
        2.07 8998 3.26%
Company A 0.84 3.7 30.28%
Company A 1.55 5.2 11.69%
Company B 1.01 12.6 2.49%
Company B 1.94 595 2.91%
Company C 0.93 116 4.62%
Company C 1.48 434 1.92%
Company D 0.85 17 5.19%
Company D 1.84 119 3.08%
* Median value for fluorescence intensity (in arbitrary units), measured
for 10,000 individual beads per sample excited at 488 nm using flow
cytometry. Values may vary slightly between batches of these products.
† CV = coefficient of variation.



On 4/19/2016 9:42 AM, John Oreopoulos wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello list,
>
> Can anyone recommend a source of yellow/green fluorescent microspheres (200 nm - 1 um diameter)? These would be for demonstration purposes, so the surface chemistry is not important to me. Looking for a fairly high concentration (maybe stock powder form?). Commercial vendors can reply to me directly offline.
>
> Sincerely,
>
> John Oreopoulos

--



George McNamara, Ph.D.
Houston, TX 77054
https://www.linkedin.com/in/georgemcnamara