Fluorescent spheres - range 100um -1000um

[Jacqueline Ross]

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Dear All,

Does anyone know of a company who can supply fluorescent spheres in this size range suitable for confocal imaging? I know people are usually looking for standards at the other end of the scale!

I've had a look at Bangs Laboratories and Spherotech but they don't seem to have anything large enough...

Kind regards,

Jacqui

Jacqueline Ross
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland 1142, NEW ZEALAND

Tel: [phone]
Fax: [phone]

http://www.fmhs.auckland.ac.nz/sms/biru/

[Franziska Curdt-2]

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You find them at Thermo Fisher (Invitrogen). E.g. Fluospheres 20nm-15um.

Kind regards,
Franziska Curdt

On Wed, Apr 20, 2016 at 6:36 AM, Jacqui Ross <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
> Does anyone know of a company who can supply fluorescent spheres in this
> size range suitable for confocal imaging? I know people are usually looking
> for standards at the other end of the scale!
>
> I've had a look at Bangs Laboratories and Spherotech but they don't seem
> to have anything large enough...
>
> Kind regards,
>
> Jacqui
>
> Jacqueline Ross
> Biomedical Imaging Microscopist
> Biomedical Imaging Research Unit
> School of Medical Sciences
> Faculty of Medical & Health Sciences
> The University of Auckland
> Private Bag 92019
> Auckland 1142, NEW ZEALAND
>
> Tel: [phone]
> Fax: [phone]
>
> http://www.fmhs.auckland.ac.nz/sms/biru/
>

[Eric Girard]

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Hi Jacqui,

Can you mold agar balls with dye, or oil dye plastic balls or silica
spacers?

Regards,

Eric Girard
Sales Engineering / Ingénierie Ventes

GiGa Concept Inc
Montreal, Quebec, Canada
Cell: 514-826-1181
Tel: [phone]
[hidden email]
www.gigaconcept.com

-----Message d'origine-----
De : Confocal Microscopy List [mailto:[hidden email]] De
la part de Jacqui Ross
Envoyé : April-20-16 12:36 AM
À : [hidden email]
Objet : Fluorescent spheres - range 100um -1000um

*****
To join, leave or search the confocal microscopy listserv, go to:
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*****

Dear All,

Does anyone know of a company who can supply fluorescent spheres in this
size range suitable for confocal imaging? I know people are usually looking
for standards at the other end of the scale!

I've had a look at Bangs Laboratories and Spherotech but they don't seem to
have anything large enough...

Kind regards,

Jacqui

Jacqueline Ross
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland 1142, NEW ZEALAND

Tel: [phone]
Fax: [phone]

http://www.fmhs.auckland.ac.nz/sms/biru/

[Knecht, David]

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We have gotten many spheres from Discovery Sciences (now Advance Scientific) http://www.advancescientific-usa.com/microparticles

Dr. David Knecht
Professor of Molecular and Cell Biology
Core Microscopy Facility Director
University of Connecticut
91 N. Eagleville Rd.
Storrs, CT 06269
860-486-2200

On Apr 20, 2016, at 4:25 AM, Eric Girard <[hidden email]<mailto:[hidden email]>> wrote:

*****
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*****

Hi Jacqui,

Can you mold agar balls with dye, or oil dye plastic balls or silica
spacers?

Regards,

Eric Girard
Sales Engineering / Ingénierie Ventes

GiGa Concept Inc
Montreal, Quebec, Canada
Cell: 514-826-1181
Tel: [phone]
[hidden email]
www.gigaconcept.com

-----Message d'origine-----
De : Confocal Microscopy List [mailto:[hidden email]] De
la part de Jacqui Ross
Envoyé : April-20-16 12:36 AM
À : [hidden email]
Objet : Fluorescent spheres - range 100um -1000um

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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*****

Dear All,

Does anyone know of a company who can supply fluorescent spheres in this
size range suitable for confocal imaging? I know people are usually looking
for standards at the other end of the scale!

I've had a look at Bangs Laboratories and Spherotech but they don't seem to
have anything large enough...

Kind regards,

Jacqui

Jacqueline Ross
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland 1142, NEW ZEALAND

Tel: [phone]
Fax: [phone]

http://www.fmhs.auckland.ac.nz/sms/biru/

[Steffen Dietzel]

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You want to check the FWHM of the PSF of your telescope? Ah, no, you
write for confocal imaging. Then I really wonder what you plan to do
with them.

Cucurbita (pumpkin, squash, zucchini) pollen is said to be ~170-180 µm
diameter, apparently the largest pollen known. If they are as
fluorescent as others, they might do the trick, depending on what you
want to do with them. Certainly would come cheaper than the latex spheres.

Steffen

Am 20.04.2016 um 06:36 schrieb Jacqui Ross:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
> Does anyone know of a company who can supply fluorescent spheres in this size range suitable for confocal imaging? I know people are usually looking for standards at the other end of the scale!
>
> I've had a look at Bangs Laboratories and Spherotech but they don't seem to have anything large enough...
>
> Kind regards,
>
> Jacqui
>
> Jacqueline Ross
> Biomedical Imaging Microscopist
> Biomedical Imaging Research Unit
> School of Medical Sciences
> Faculty of Medical & Health Sciences
> The University of Auckland
> Private Bag 92019
> Auckland 1142, NEW ZEALAND
>
> Tel: [phone]
> Fax: [phone]
>
> http://www.fmhs.auckland.ac.nz/sms/biru/
>
>
> --
> ------------------------------------------------------------
> Steffen Dietzel, PD Dr. rer. nat
> Ludwig-Maximilians-Universität München
> Biomedical Center (BMC)
> Head of the Core Facility Bioimaging
>
> Großhaderner Straße 9
> D-82152 Planegg-Martinsried
> Germany
>
> http://www.bioimaging.bmc.med.uni-muenchen.de

[Jacqueline Ross]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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*****

Thanks very much to everyone who replied to my email about fluorescent spheres,

I now have a number of different avenues (and techniques) to try. I may even head down to the community garden and see if we still have any pumpkin/zucchini pollen available as suggested by Steffen:-).

Cheers,

Jacqui

Jacqueline Ross
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit 
School of Medical Sciences 
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland 1142, NEW ZEALAND

Tel: [phone]
Fax: [phone]

http://www.fmhs.auckland.ac.nz/sms/biru/


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Steffen Dietzel
Sent: Thursday, 21 April 2016 2:21 a.m.
To: [hidden email]
Subject: Re: Fluorescent spheres - range 100um -1000um

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

You want to check the FWHM of the PSF of your telescope? Ah, no, you
write for confocal imaging. Then I really wonder what you plan to do
with them.

Cucurbita (pumpkin, squash, zucchini) pollen is said to be ~170-180 µm
diameter, apparently the largest pollen known. If they are as
fluorescent as others, they might do the trick, depending on what you
want to do with them. Certainly would come cheaper than the latex spheres.

Steffen

Am 20.04.2016 um 06:36 schrieb Jacqui Ross:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
> Does anyone know of a company who can supply fluorescent spheres in this size range suitable for confocal imaging? I know people are usually looking for standards at the other end of the scale!
>
> I've had a look at Bangs Laboratories and Spherotech but they don't seem to have anything large enough...
>
> Kind regards,
>
> Jacqui
>
> Jacqueline Ross
> Biomedical Imaging Microscopist
> Biomedical Imaging Research Unit
> School of Medical Sciences
> Faculty of Medical & Health Sciences
> The University of Auckland
> Private Bag 92019
> Auckland 1142, NEW ZEALAND
>
> Tel: [phone]
> Fax: [phone]
>
> http://www.fmhs.auckland.ac.nz/sms/biru/
>
>
> --
> ------------------------------------------------------------
> Steffen Dietzel, PD Dr. rer. nat
> Ludwig-Maximilians-Universität München
> Biomedical Center (BMC)
> Head of the Core Facility Bioimaging
>
> Großhaderner Straße 9
> D-82152 Planegg-Martinsried
> Germany
>
> http://www.bioimaging.bmc.med.uni-muenchen.de