Fluorophores for wood?
Locked
|
Dear Confocal community,
I have a special question regarding labeling of wood cells (spruce). For tomography reasons I like to label wood cells to improve the autofluorescence, because not all cell structures might give a constant signal. So far we have managed to visualize a wood decaying fungus that grows through the pits and erodes the cellwalls. Which dyes and fluorophores might bind to all elements of wood (lignin, cellulose, hemicellulose) to produce a constant fluorescence? Thanks for any helpful comments!! best wishes Chris Stührk Laboratory Wood Woodprotection and Biotechnology Empa - Material Science & Technology Room D 1.17 Lerchenfeldstrasse 5 CH-9014 St. Gallen Pho: +41 (0) 71 2747622 Fax: +41 (0) 71 2747694 [hidden email] |
|
|
Chris,
Constancy is a bit of a trick. I don't know that anything would be perfectly constant. A popular stain is calcofluor white. This stains cellulose and other polysaccharides. It is uv excited, blue emission. All cell walls will be stained although there will be some variation in brightness. Hope this helps, Tobias >Dear Confocal community, > >I have a special question regarding labeling of wood cells (spruce). >For tomography reasons I like to label wood >cells to improve the autofluorescence, because >not all >cell structures might give a constant signal. >So far we have managed to visualize a wood decaying fungus that grows through >the pits and erodes the cellwalls. >Which dyes and fluorophores might bind to all >elements of wood (lignin, cellulose, >hemicellulose) >to produce a constant fluorescence? >Thanks for any helpful comments!! > >best wishes > >Chris Stührk > >Laboratory Wood >Woodprotection and Biotechnology >Empa - Material Science & Technology >Room D 1.17 >Lerchenfeldstrasse 5 >CH-9014 St. Gallen > >Pho: +41 (0) 71 2747622 >Fax: +41 (0) 71 2747694 >[hidden email] -- _ ____ __ ____ / \ / / \ / \ \ Tobias I. Baskin / / / / \ \ \ Biology Department /_ / __ /__ \ \ \__ 611 N. Pleasant St. / / / \ \ \ University of Massachusetts / / / \ \ \ Amherst, MA, 01003 / / ___ / \ \__/ \ ____ www.bio.umass.edu/biology/baskin Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243 |
|
|
In reply to this post by Stuehrk, Chris
Hi Tobias,
thanks for your hint! best wishes Chris |
|
|
In reply to this post by Stuehrk, Chris
Chris
There are a range of things you could try. Acriflavin, acridine orange, safranine, berberine sulphate, toluidine blue, basic fuchsin and any schiff reagent. These will all tend to stain lignified walls much more than unlignified walls although with limited specificity. We have used most of these for 3D reconstructions. We have a CD available detailing techniques and examples of reconstructions. Contact me off list if you are interested. Regards Dr Lloyd Donaldson Senior Scientist Scion - Next Generation Biomaterials 49 Sala St. Rotorua Private Bag 3020, Rotorua 3046 NEW ZEALAND Ph: 64 7 343 5581 Fx: 64 7 343 5507 www.scionresearch.com -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Stuehrk, Chris Sent: Wednesday, 27 January 2010 5:07 a.m. To: [hidden email] Subject: Fluorophores for wood? Dear Confocal community, I have a special question regarding labeling of wood cells (spruce). For tomography reasons I like to label wood cells to improve the autofluorescence, because not all cell structures might give a constant signal. So far we have managed to visualize a wood decaying fungus that grows through the pits and erodes the cellwalls. Which dyes and fluorophores might bind to all elements of wood (lignin, cellulose, hemicellulose) to produce a constant fluorescence? Thanks for any helpful comments!! best wishes Chris Stührk Laboratory Wood Woodprotection and Biotechnology Empa - Material Science & Technology Room D 1.17 Lerchenfeldstrasse 5 CH-9014 St. Gallen Pho: +41 (0) 71 2747622 Fax: +41 (0) 71 2747694 [hidden email] Disclaimer: This e-mail and any attachments may contain information which is confidential or subject to copyright. If you receive this e-mail in error, please delete it. Scion does not accept responsibility for anything in this e-mail which is not provided in the course of Scion's usual business or for any computer virus, data corruption, interference or delay arising from this e-mail. |
|
|
Dear Prof Donaldson,
naturally, Im interested in your CD!! with the best wishes Chris Stührk ________________________________________ Von: Confocal Microscopy List [[hidden email]] im Auftrag von Lloyd Donaldson [[hidden email]] Gesendet: Dienstag, 26. Januar 2010 20:02 An: [hidden email] Betreff: Re: Fluorophores for wood? Chris There are a range of things you could try. Acriflavin, acridine orange, safranine, berberine sulphate, toluidine blue, basic fuchsin and any schiff reagent. These will all tend to stain lignified walls much more than unlignified walls although with limited specificity. We have used most of these for 3D reconstructions. We have a CD available detailing techniques and examples of reconstructions. Contact me off list if you are interested. Regards Dr Lloyd Donaldson Senior Scientist Scion - Next Generation Biomaterials 49 Sala St. Rotorua Private Bag 3020, Rotorua 3046 NEW ZEALAND Ph: 64 7 343 5581 Fx: 64 7 343 5507 www.scionresearch.com -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Stuehrk, Chris Sent: Wednesday, 27 January 2010 5:07 a.m. To: [hidden email] Subject: Fluorophores for wood? Dear Confocal community, I have a special question regarding labeling of wood cells (spruce). For tomography reasons I like to label wood cells to improve the autofluorescence, because not all cell structures might give a constant signal. So far we have managed to visualize a wood decaying fungus that grows through the pits and erodes the cellwalls. Which dyes and fluorophores might bind to all elements of wood (lignin, cellulose, hemicellulose) to produce a constant fluorescence? Thanks for any helpful comments!! best wishes Chris Stührk Laboratory Wood Woodprotection and Biotechnology Empa - Material Science & Technology Room D 1.17 Lerchenfeldstrasse 5 CH-9014 St. Gallen Pho: +41 (0) 71 2747622 Fax: +41 (0) 71 2747694 [hidden email] Disclaimer: This e-mail and any attachments may contain information which is confidential or subject to copyright. If you receive this e-mail in error, please delete it. Scion does not accept responsibility for anything in this e-mail which is not provided in the course of Scion's usual business or for any computer virus, data corruption, interference or delay arising from this e-mail. |
«
Return to Confocal Microscopy List
|
1 view|%1 views
| Free forum by Nabble | Edit this page |