Free Nyquist Calculator App

[Vincent Schoonderwoert]

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**commercial posting**


Dear list,

For many years we have a freely accessible Nyquist Calculator on our
website (http://www.svi.nl/NyquistCalculator), which Jens was kindly
referring to in a recent post.
This Nyquist Calculator determines the theoretical sampling density for
your microscopic-image acquisition. With the computed sampling density
you will make optimal use of the resolution of your microscope and
minimize image distortions (apologies for the broad term). Additionally,
the Nyquist Calculator can visualize the Point Spread Function (PSF) for
various microscope types and parameters.

Apparently, there is a need for such a calculator since the Nyquist
Calculator page has a high number of visitors. Encouraged by this
apparent need and the many requests from our users, we are pleased to
announce the Nyquist Calculator app for Android, available immediately.
As of yet, the Android app does not calculate PSFs, but the app is in
active development and ideas and feedback are welcome.

Install the app now from
https://play.google.com/store/apps/details?id=com.svi.nyquistcalculator.app

Kind regards from SVI,

Vincent Schoonderwoert


Submit your most beautiful images for the Huygens Image Contest and win a Nexus 9 tablet - www.svi.nl

*****************************************
Vincent Schoonderwoert, PhD
Senior Imaging Specialist/Account Manager
Scientific Volume Imaging
www.svi.nl
*****************************************

[Loralei Dewe-3]

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*****

Really nice!!  Installed perfectly!!

Loralei Dewe
Chrysalis Innovations
On Nov 19, 2014 6:41 AM, "Vincent Schoonderwoert" <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> **commercial posting**
>
>
> Dear list,
>
> For many years we have a freely accessible Nyquist Calculator on our
> website (http://www.svi.nl/NyquistCalculator), which Jens was kindly
> referring to in a recent post.
> This Nyquist Calculator determines the theoretical sampling density for
> your microscopic-image acquisition. With the computed sampling density you
> will make optimal use of the resolution of your microscope and minimize
> image distortions (apologies for the broad term). Additionally, the Nyquist
> Calculator can visualize the Point Spread Function (PSF) for various
> microscope types and parameters.
>
> Apparently, there is a need for such a calculator since the Nyquist
> Calculator page has a high number of visitors. Encouraged by this apparent
> need and the many requests from our users, we are pleased to announce the
> Nyquist Calculator app for Android, available immediately.
> As of yet, the Android app does not calculate PSFs, but the app is in
> active development and ideas and feedback are welcome.
>
> Install the app now from https://play.google.com/store/
> apps/details?id=com.svi.nyquistcalculator.app
>
> Kind regards from SVI,
>
> Vincent Schoonderwoert
>
>
> Submit your most beautiful images for the Huygens Image Contest and win a
> Nexus 9 tablet - www.svi.nl
>
> *****************************************
> Vincent Schoonderwoert, PhD
> Senior Imaging Specialist/Account Manager
> Scientific Volume Imaging
> www.svi.nl
> *****************************************
>

[John Oreopoulos]

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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Vincent,

What a great tool to have at an arm's reach! ... Now any chance we'll see an iPhone version? ;)

John Oreopoulos

> On Nov 19, 2014, at 9:38 AM, Vincent Schoonderwoert <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> **commercial posting**
>
>
> Dear list,
>
> For many years we have a freely accessible Nyquist Calculator on our website (http://www.svi.nl/NyquistCalculator), which Jens was kindly referring to in a recent post.
> This Nyquist Calculator determines the theoretical sampling density for your microscopic-image acquisition. With the computed sampling density you will make optimal use of the resolution of your microscope and minimize image distortions (apologies for the broad term). Additionally, the Nyquist Calculator can visualize the Point Spread Function (PSF) for various microscope types and parameters.
>
> Apparently, there is a need for such a calculator since the Nyquist Calculator page has a high number of visitors. Encouraged by this apparent need and the many requests from our users, we are pleased to announce the Nyquist Calculator app for Android, available immediately.
> As of yet, the Android app does not calculate PSFs, but the app is in active development and ideas and feedback are welcome.
>
> Install the app now from https://play.google.com/store/apps/details?id=com.svi.nyquistcalculator.app
>
> Kind regards from SVI,
>
> Vincent Schoonderwoert
>
>
> Submit your most beautiful images for the Huygens Image Contest and win a Nexus 9 tablet - www.svi.nl
>
> *****************************************
> Vincent Schoonderwoert, PhD
> Senior Imaging Specialist/Account Manager
> Scientific Volume Imaging
> www.svi.nl
> *****************************************

[Jeremy Adler-4]

It is very good of SVI to provide their Nyquist Caclulator.

An inconvenience is inputting the backprojected pinhole radius - It's not a number that immediately comes to mind or is obvious on many confocals. Would it be possible to input the pinhole size in Airy units and then apply a correction for common confocals with round or square pinholes ?


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Vincent Schoonderwoert
Sent: den 19 november 2014 15:39
To: [hidden email]
Subject: Free Nyquist Calculator App

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

**commercial posting**


Dear list,

For many years we have a freely accessible Nyquist Calculator on our website (http://www.svi.nl/NyquistCalculator), which Jens was kindly referring to in a recent post.
This Nyquist Calculator determines the theoretical sampling density for your microscopic-image acquisition. With the computed sampling density you will make optimal use of the resolution of your microscope and minimize image distortions (apologies for the broad term). Additionally, the Nyquist Calculator can visualize the Point Spread Function (PSF) for various microscope types and parameters.

Apparently, there is a need for such a calculator since the Nyquist Calculator page has a high number of visitors. Encouraged by this apparent need and the many requests from our users, we are pleased to announce the Nyquist Calculator app for Android, available immediately.
As of yet, the Android app does not calculate PSFs, but the app is in active development and ideas and feedback are welcome.

Install the app now from
https://play.google.com/store/apps/details?id=com.svi.nyquistcalculator.app

Kind regards from SVI,

Vincent Schoonderwoert


Submit your most beautiful images for the Huygens Image Contest and win a Nexus 9 tablet - www.svi.nl

*****************************************
Vincent Schoonderwoert, PhD
Senior Imaging Specialist/Account Manager Scientific Volume Imaging www.svi.nl
*****************************************

[olivier.burri]

Dear Jeremy,

You can read the decision from SVI not to consider the influence of the pinhole on the Nyquist calculations here.

http://www.svi.nl/PinholeAndBandwidth


It was not too clear for me either, but this helped quite a bit

Best

Oli

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Jeremy Adler
Sent: Thursday, November 20, 2014 10:57 AM
To: [hidden email]
Subject: Re: Free Nyquist Calculator App

It is very good of SVI to provide their Nyquist Caclulator.

An inconvenience is inputting the backprojected pinhole radius - It's not a number that immediately comes to mind or is obvious on many confocals. Would it be possible to input the pinhole size in Airy units and then apply a correction for common confocals with round or square pinholes ?


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Vincent Schoonderwoert
Sent: den 19 november 2014 15:39
To: [hidden email]
Subject: Free Nyquist Calculator App

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

**commercial posting**


Dear list,

For many years we have a freely accessible Nyquist Calculator on our website (http://www.svi.nl/NyquistCalculator), which Jens was kindly referring to in a recent post.
This Nyquist Calculator determines the theoretical sampling density for your microscopic-image acquisition. With the computed sampling density you will make optimal use of the resolution of your microscope and minimize image distortions (apologies for the broad term). Additionally, the Nyquist Calculator can visualize the Point Spread Function (PSF) for various microscope types and parameters.

Apparently, there is a need for such a calculator since the Nyquist Calculator page has a high number of visitors. Encouraged by this apparent need and the many requests from our users, we are pleased to announce the Nyquist Calculator app for Android, available immediately.
As of yet, the Android app does not calculate PSFs, but the app is in active development and ideas and feedback are welcome.

Install the app now from
https://play.google.com/store/apps/details?id=com.svi.nyquistcalculator.app

Kind regards from SVI,

Vincent Schoonderwoert


Submit your most beautiful images for the Huygens Image Contest and win a Nexus 9 tablet - www.svi.nl

*****************************************
Vincent Schoonderwoert, PhD
Senior Imaging Specialist/Account Manager Scientific Volume Imaging www.svi.nl
*****************************************

[Vincent Schoonderwoert]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear John,

Thanks, but the Santa you have to thank is Gerhard Burger, one of our
ace developers. This year, Santa was into Android ;-)

At an early stage, we have supported this app as we recognize that
sampling is too often compromised for the sake of enough signal. We hope
the app will be used by many and that it will give proper sampling a
higher priority - even if that means lower signal - when studies concern
e.g. intensity, structural, and localization measurements. Proper
sampling also benefits deconvolution (apologies for the twist at the end).

Vincent

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Vincent,
>
> What a great tool to have at an arm's reach! ... Now any chance we'll see an iPhone version? ;)
>
> John Oreopoulos
>
>> On Nov 19, 2014, at 9:38 AM, Vincent Schoonderwoert <[hidden email]> wrote:
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> **commercial posting**
>>
>>
>> Dear list,
>>
>> For many years we have a freely accessible Nyquist Calculator on our website (http://www.svi.nl/NyquistCalculator), which Jens was kindly referring to in a recent post.
>> This Nyquist Calculator determines the theoretical sampling density for your microscopic-image acquisition. With the computed sampling density you will make optimal use of the resolution of your microscope and minimize image distortions (apologies for the broad term). Additionally, the Nyquist Calculator can visualize the Point Spread Function (PSF) for various microscope types and parameters.
>>
>> Apparently, there is a need for such a calculator since the Nyquist Calculator page has a high number of visitors. Encouraged by this apparent need and the many requests from our users, we are pleased to announce the Nyquist Calculator app for Android, available immediately.
>> As of yet, the Android app does not calculate PSFs, but the app is in active development and ideas and feedback are welcome.
>>
>> Install the app now from https://play.google.com/store/apps/details?id=com.svi.nyquistcalculator.app
>>
>> Kind regards from SVI,
>>
>> Vincent Schoonderwoert
>>
>>
>> Submit your most beautiful images for the Huygens Image Contest and win a Nexus 9 tablet - www.svi.nl
>>
>> *****************************************
>> Vincent Schoonderwoert, PhD
>> Senior Imaging Specialist/Account Manager
>> Scientific Volume Imaging
>> www.svi.nl
>> *****************************************

--
Submit your most beautiful images for the Huygens Image Contest and win a Nexus 9 tablet - www.svi.nl

*****************************************
Vincent Schoonderwoert, PhD
Senior Imaging Specialist/Account Manager
Scientific Volume Imaging
www.svi.nl
*****************************************

[Zdenek Svindrych]

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*****

Dear Oli and others,
The paragraph on Bandwidth and Resolution you've just pointed out is
misleading. It states that the confocal OTF is twice as wide, so we should
call it a super-resolution technique, shouldn't we? The 'linear' superres
techniques (SIM, SMI, ISM, ...) also aim at the ultimate factor-of-two
widening of the OTF bandwidth.

Quote: "Still, for different pinhole sizes, the OTF becomes truly zero at
exactly the same frequency, leaving the bandwidth unchanged." Does that mean
that confocal is superres even with the pinhole open wide? Surely not. Or
maybe...??? One would expect that the bandwidth should decrease rapidly as
one goes from zero pinhole (factor-of-two superresolution) to infinite
pinhole (normal widefield).
To prove SVI wrong I did a simple calculation and, indeed, they are right!
The overall bandwidth does NOT decrease as the pinhole opens because the
model pinhole is infinitely sharp, so it has infinite 'bandwidth' in
frequency space (although with many zeros and very small amplitudes) so it
formally does not affect the detection OTF bandwidth.

Conclusion? Not sure. It is very tricky to speak about 'ultimate bandwidth'
and limiting cases. I think it's OK to call AiryScan a superres technique
and normal confocal as non-superres... 

Maybe the concept of Stochastic Transfer Function (you can google some
Somekh's papers about it) would help here.

Best, zdenek



---------- Původní zpráva ----------
Od: Burri Olivier <[hidden email]>
Komu: [hidden email]
Datum: 20. 11. 2014 7:00:58
Předmět: Re: Free Nyquist Calculator App

"Dear Jeremy,

You can read the decision from SVI not to consider the influence of the
pinhole on the Nyquist calculations here.

http://www.svi.nl/PinholeAndBandwidth


It was not too clear for me either, but this helped quite a bit

Best

Oli

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Jeremy Adler
Sent: Thursday, November 20, 2014 10:57 AM
To: [hidden email]
Subject: Re: Free Nyquist Calculator App

It is very good of SVI to provide their Nyquist Caclulator.

An inconvenience is inputting the backprojected pinhole radius - It's not a
number that immediately comes to mind or is obvious on many confocals. Would
it be possible to input the pinhole size in Airy units and then apply a
correction for common confocals with round or square pinholes ?


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Vincent Schoonderwoert
Sent: den 19 november 2014 15:39
To: [hidden email]
Subject: Free Nyquist Calculator App

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

**commercial posting**


Dear list,

For many years we have a freely accessible Nyquist Calculator on our website
(http://www.svi.nl/NyquistCalculator), which Jens was kindly referring to in
a recent post.
This Nyquist Calculator determines the theoretical sampling density for your
microscopic-image acquisition. With the computed sampling density you will
make optimal use of the resolution of your microscope and minimize image
distortions (apologies for the broad term). Additionally, the Nyquist
Calculator can visualize the Point Spread Function (PSF) for various
microscope types and parameters.

Apparently, there is a need for such a calculator since the Nyquist
Calculator page has a high number of visitors. Encouraged by this apparent
need and the many requests from our users, we are pleased to announce the
Nyquist Calculator app for Android, available immediately.
As of yet, the Android app does not calculate PSFs, but the app is in active
development and ideas and feedback are welcome.

Install the app now from
https://play.google.com/store/apps/details?id=com.svi.nyquistcalculator.app

Kind regards from SVI,

Vincent Schoonderwoert


Submit your most beautiful images for the Huygens Image Contest and win a
Nexus 9 tablet - www.svi.nl

*****************************************
Vincent Schoonderwoert, PhD
Senior Imaging Specialist/Account Manager Scientific Volume Imaging www.svi.
nl
*****************************************"

[Sudipta Maiti]

*****
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Friends,
I draw your attention to the 6th workshop in the series of Annual FCS
workshops, to be held in Pune, India, from Dec. 15 to 19, 2014, with a
satellite workshop from Dec. 13-14. The workshop has gone far beyond FCS,
and now covers most techniques in Fluorescence and Raman (it has been
rechristened FluoRa this year). There will be a module on AFM too. There are
extensive teaching and hands on sessions  on the techniques, and a separate
session on  research topics.

Please find more at www.fcsworkshop.in.

If want to really learn the ins and  outs of these techniques, please apply
soon: the seats are limited.
Sudipta


Prof. Sudipta Maiti
Dept. of Chemical Sciences
Tata Institute of Fundamental Research
Homi Bhabha Road, Colaba
Mumbai 400005, India
Ph. [phone]
Alternate e-mail: [hidden email]
webpage: biophotonics.co.in