Freeware image acquisition and Coverslip marking

[Farid Jalali]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello Group,
1) Can anyone offer a suggestion for free/share-ware to drive simple image acquisition using a Sony XCD-X700 IEEE 1394 camera? Its attached to our Axiovert 100 and we use it for basic brightfield/ phase contrast fluorescence image acquisition.
2) Does anyone have a suggestion for marking #1.5 coverslips more permanently? I have tried a diamond tipped pen but the score was too deep and media made its way through. We have tried nail polish, but it seems to eventually wash away as does permanent marker. I am generating laser-mediated tracks of DNA damage and want to be able to orient my coverslips after I have fixed and stained the samples.

As always, any help or suggestions would be greatly appreciated.
Cheers
Farid
--
Farid Jalali MSc
Senior Research Technician/ Lab Manager
Dr. Robert Bristow Lab
Applied Molecular Oncology
Princess Margaret Hospital
Toronto, Canada
416-946-4501 X4351 (Princess Margaret Hospital)
416-581-7754 STTARR at MaRS Building
416-581-7791 STTARR Microscopy Suite

[Knecht, David]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal We use Micro-manager (free) to control the Sony firewire camera.  It also works with BTV Pro (cheap).   Dave

On Feb 19, 2008, at 8:04 PM, Farid Jalali wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello Group,
1) Can anyone offer a suggestion for free/share-ware to drive simple image acquisition using a Sony XCD-X700 IEEE 1394 camera? Its attached to our Axiovert 100 and we use it for basic brightfield/ phase contrast fluorescence image acquisition.
2) Does anyone have a suggestion for marking #1.5 coverslips more permanently? I have tried a diamond tipped pen but the score was too deep and media made its way through. We have tried nail polish, but it seems to eventually wash away as does permanent marker. I am generating laser-mediated tracks of DNA damage and want to be able to orient my coverslips after I have fixed and stained the samples.

As always, any help or suggestions would be greatly appreciated.
Cheers
Farid
--
Farid Jalali MSc
Senior Research Technician/ Lab Manager
Dr. Robert Bristow Lab
Applied Molecular Oncology
Princess Margaret Hospital
Toronto, Canada
416-946-4501 X4351 (Princess Margaret Hospital)
416-581-7754 STTARR at MaRS Building
416-581-7791 STTARR Microscopy Suite

Dr. David Knecht    

Department of Molecular and Cell Biology

Co-head Flow Cytometry and Confocal Microscopy Facility

U-3125

91 N. Eagleville Rd.

University of Connecticut

Storrs, CT 06269

860-486-2200

860-486-4331 (fax)

[lechristophe]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

I'd be very interested to hear answers about the fiducial mark issue too...

Christophe Leterrier

On Feb 20, 2008 2:04 AM, Farid Jalali <[hidden email]> wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello Group,
> 1) Can anyone offer a suggestion for free/share-ware to drive simple image
> acquisition using a Sony XCD-X700 IEEE 1394 camera? Its attached to our
> Axiovert 100 and we use it for basic brightfield/ phase contrast
> fluorescence image acquisition.
>  2) Does anyone have a suggestion for marking #1.5 coverslips more
> permanently? I have tried a diamond tipped pen but the score was too deep
> and media made its way through. We have tried nail polish, but it seems to
> eventually wash away as does permanent marker. I am generating
> laser-mediated tracks of DNA damage and want to be able to orient my
> coverslips after I have fixed and stained the samples.
>
> As always, any help or suggestions would be greatly appreciated.
> Cheers
> Farid
> --
> Farid Jalali MSc
> Senior Research Technician/ Lab Manager
> Dr. Robert Bristow Lab
> Applied Molecular Oncology
> Princess Margaret Hospital
>  Toronto, Canada
> 416-946-4501 X4351 (Princess Margaret Hospital)
> 416-581-7754 STTARR at MaRS Building
> 416-581-7791 STTARR Microscopy Suite

[Michael Weber-4]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Dear Farid and Christophe,

I am not sure if this is useful for you, but have a look at the Ibidi
grid dish:

http://ibidi.com/products/dish_grid.html

This might help to locate the sample over the whole process of culturing
and acquisition. Maybe it's an option to home-made solutions.

I am pretty confident that some time ago, I also saw prepared coverslips
like this, but don't remember where.

Michael


Christophe Leterrier wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> I'd be very interested to hear answers about the fiducial mark issue too...
>
> Christophe Leterrier
>
> On Feb 20, 2008 2:04 AM, Farid Jalali <[hidden email]> wrote:
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello Group,
>> 1) Can anyone offer a suggestion for free/share-ware to drive simple image
>> acquisition using a Sony XCD-X700 IEEE 1394 camera? Its attached to our
>> Axiovert 100 and we use it for basic brightfield/ phase contrast
>> fluorescence image acquisition.
>>  2) Does anyone have a suggestion for marking #1.5 coverslips more
>> permanently? I have tried a diamond tipped pen but the score was too deep
>> and media made its way through. We have tried nail polish, but it seems to
>> eventually wash away as does permanent marker. I am generating
>> laser-mediated tracks of DNA damage and want to be able to orient my
>> coverslips after I have fixed and stained the samples.
>>
>> As always, any help or suggestions would be greatly appreciated.
>> Cheers
>> Farid
>> --
>> Farid Jalali MSc
>> Senior Research Technician/ Lab Manager
>> Dr. Robert Bristow Lab
>> Applied Molecular Oncology
>> Princess Margaret Hospital
>>  Toronto, Canada
>> 416-946-4501 X4351 (Princess Margaret Hospital)
>> 416-581-7754 STTARR at MaRS Building
>> 416-581-7791 STTARR Microscopy Suite

[Lingqing Zhang]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi, Farid,

 

You could try to use high power laser to mark coverslips, as the most simple example of laser micro-machining.

 

Lingqing

 

On 2/20/08, Michael Weber <[hidden email]> wrote:

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Dear Farid and Christophe,

I am not sure if this is useful for you, but have a look at the Ibidi
grid dish:

http://ibidi.com/products/dish_grid.html

This might help to locate the sample over the whole process of culturing
and acquisition. Maybe it's an option to home-made solutions.

I am pretty confident that some time ago, I also saw prepared coverslips
like this, but don't remember where.

Michael


Christophe Leterrier wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> I'd be very interested to hear answers about the fiducial mark issue too...
>
> Christophe Leterrier
>
> On Feb 20, 2008 2:04 AM, Farid Jalali <[hidden email]> wrote:
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello Group,
>> 1) Can anyone offer a suggestion for free/share-ware to drive simple image
>> acquisition using a Sony XCD-X700 IEEE 1394 camera? Its attached to our
>> Axiovert 100 and we use it for basic brightfield/ phase contrast
>> fluorescence image acquisition.
>>  2) Does anyone have a suggestion for marking #1.5 coverslips more
>> permanently? I have tried a diamond tipped pen but the score was too deep
>> and media made its way through. We have tried nail polish, but it seems to
>> eventually wash away as does permanent marker. I am generating
>> laser-mediated tracks of DNA damage and want to be able to orient my
>> coverslips after I have fixed and stained the samples.
>>
>> As always, any help or suggestions would be greatly appreciated.
>> Cheers
>> Farid
>> --
>> Farid Jalali MSc
>> Senior Research Technician/ Lab Manager
>> Dr. Robert Bristow Lab
>> Applied Molecular Oncology
>> Princess Margaret Hospital
>>  Toronto, Canada
>> 416-946-4501 X4351 (Princess Margaret Hospital)
>> 416-581-7754 STTARR at MaRS Building
>> 416-581-7791 STTARR Microscopy Suite

[cromey]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

My histotech labels her slides with marking pens #01880 (ultra fine point) from SURGIPATH.  This permanent marker holds up to trips through xylene and 100% ethanol.  There are other such pens out there for histology labs.

 

http://www.surgipath.com/region/us/product.aspx?p=33

 

I have no affiliation with Surgipath.

 

Can’t help you with the camera.

 

Doug

 

^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^

Douglas W. Cromey, M.S. - Assistant Scientific Investigator

Dept. of Cell Biology & Anatomy, University of Arizona

1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA

 

office:  AHSC 4212         email: [hidden email]

voice:  520-626-2824       fax:  [phone]

 

http://swehsc.pharmacy.arizona.edu/exppath/

Home of: "Microscopy and Imaging Resources on the WWW"

 

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Farid Jalali
Sent: Tuesday, February 19, 2008 6:05 PM
To: [hidden email]
Subject: Freeware image acquisition and Coverslip marking

 

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello Group,
1) Can anyone offer a suggestion for free/share-ware to drive simple image acquisition using a Sony XCD-X700 IEEE 1394 camera? Its attached to our Axiovert 100 and we use it for basic brightfield/ phase contrast fluorescence image acquisition.
2) Does anyone have a suggestion for marking #1.5 coverslips more permanently? I have tried a diamond tipped pen but the score was too deep and media made its way through. We have tried nail polish, but it seems to eventually wash away as does permanent marker. I am generating laser-mediated tracks of DNA damage and want to be able to orient my coverslips after I have fixed and stained the samples.

As always, any help or suggestions would be greatly appreciated.
Cheers
Farid
--
Farid Jalali MSc
Senior Research Technician/ Lab Manager
Dr. Robert Bristow Lab
Applied Molecular Oncology
Princess Margaret Hospital
Toronto, Canada
416-946-4501 X4351 (Princess Margaret Hospital)
416-581-7754 STTARR at MaRS Building
416-581-7791 STTARR Microscopy Suite