General fluorescent stain for cytoplasm

[Eric Shelden]

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All:

I am looking for a way to fluorescently counter-stain the volume of cultured
cells, with a result similar to what would be achieved by having them
express GFP in the cytoplasm. I have seen some references to staining with
eosin Y. Does anyone have other suggestions?

Thanks in advance.

Eric

[Cromey, Douglas W - (dcromey)]

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Invitrogen's Live/Dead assay includes calcein, a dye that is only taken up by live cells and it only becomes fluorescent when it is exposed to cytoplasmic esterases.  It's a green dye and I am pretty sure that you can purchase it separately and not just as part of the kit.
Doug

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-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Eric Shelden
Sent: Wednesday, September 17, 2014 10:49 AM
To: [hidden email]
Subject: General fluorescent stain for cytoplasm

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All:

I am looking for a way to fluorescently counter-stain the volume of cultured cells, with a result similar to what would be achieved by having them express GFP in the cytoplasm. I have seen some references to staining with eosin Y. Does anyone have other suggestions?

Thanks in advance.

Eric

[Chew, Teng-Leong]

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Invitrogen: CellTracer (several colors to choose from).

Best,
Leong


--
Teng-Leong Chew, PhD
Director, Advanced Imaging Center
Howard Hughes Medical Institute at Janelia
Ashburn, VA 20147









On 9/17/14, 1:49 PM, "Eric Shelden" <[hidden email]> wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>Post images on http://www.imgur.com and include the link in your posting.
>*****
>
>All:
>
>I am looking for a way to fluorescently counter-stain the volume of
>cultured
>cells, with a result similar to what would be achieved by having them
>express GFP in the cytoplasm. I have seen some references to staining
>with
>eosin Y. Does anyone have other suggestions?
>
>Thanks in advance.
>
>Eric