F Javier Diez Guerra |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear confocalists, We have several cameras that have accumulated dust on or near the sensor, and will appreciate any advice on how to get rid of those annoying specks that degrade images so much. We do not have a clean room in our building. Thanks in advance for your input. Javier -- Fco. Javier Diez-Guerra, PhD Servicio de Microscopía Confocal Centro de Biologia Molecular Severo Ochoa C/ Nicolás Cabrera, 1 Campus de Cantoblanco 28049 Madrid SPAIN Tel +34 91 196 4612 e-mail: [hidden email] |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Javier, The dust is probably on the glass window covering the sensor. I used to clean it the same way as any other glass, with a mild solvent Mike -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of F Javier Diez Guerra Sent: Thursday, October 8, 2020 4:20 AM To: [hidden email] Subject: Get rid of dust in camera sensor ***** To join, leave or search the confocal microscopy listserv, go to: https://nam03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=02%7C01%7Cmmodel%40KENT.EDU%7C7e0557a2214f442f00ce08d86b630690%7Ce5a06f4a1ec44d018f73e7dd15f26134%7C1%7C0%7C637377420338521101&sdata=7lM9R0eis7Ut1v%2BRxTK0m%2FwTcA570FkyOTUCaQBLEj8%3D&reserved=0 Post images on https://nam03.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=02%7C01%7Cmmodel%40KENT.EDU%7C7e0557a2214f442f00ce08d86b630690%7Ce5a06f4a1ec44d018f73e7dd15f26134%7C1%7C0%7C637377420338521101&sdata=hIJcnoo0EQZMva32%2B0QcReRXSayI527T0U8ng7du%2FC4%3D&reserved=0 and include the link in your posting. ***** Dear confocalists, We have several cameras that have accumulated dust on or near the sensor, and will appreciate any advice on how to get rid of those annoying specks that degrade images so much. We do not have a clean room in our building. Thanks in advance for your input. Javier -- Fco. Javier Diez-Guerra, PhD Servicio de Microscopía Confocal Centro de Biologia Molecular Severo Ochoa C/ Nicolás Cabrera, 1 Campus de Cantoblanco 28049 Madrid SPAIN Tel +34 91 196 4612 e-mail: [hidden email] |
Gerhard Holst |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Mike and Javier, in case the dust is on the entrance glass window of a cooled scientific camera, the suggestion of mike is valuable, I would assume you can do the same like the cleaning of your expensive microscope objectives. Since Javier is annoyed by the dust signal in the images, it can as well be that the dust is directly attached to the micro lenses on the image sensor or on the cover glass of the image sensor, meaning in the vicinity of the pixels, which makes the dust sharply visible on all images with light. Third possibility is, condensation, this creates small annoying bubbles on each image. And depending on the origin you can clean yourself gently, or you will have to contact the manufacturer. Best regards, Gerhard Dr. Gerhard Holst Head of Research & Science Business Development +49 (0) 9441 2005 570 PCO AG, Donaupark 11, 93309 Kelheim, Germany, www.pco.de Vorstand / Managing Board: Alexander Grünig, Luitpold Kaspar Vorsitzender des Aufsichtsrats / Chairman of the Supervisory Board: Dr. Emil Ott USt. ID-Nr. / VAT: DE128590843, Registergericht / Register Court: Amtsgericht Regensburg HRB 9157 Sitz der Gesellschaft / Registered Office: Kelheim -----Ursprüngliche Nachricht----- Von: Confocal Microscopy List <[hidden email]> Im Auftrag von MODEL, MICHAEL Gesendet: Donnerstag, 8. Oktober 2020 13:26 An: [hidden email] Betreff: Re: Get rid of dust in camera sensor ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Javier, The dust is probably on the glass window covering the sensor. I used to clean it the same way as any other glass, with a mild solvent Mike -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of F Javier Diez Guerra Sent: Thursday, October 8, 2020 4:20 AM To: [hidden email] Subject: Get rid of dust in camera sensor ***** To join, leave or search the confocal microscopy listserv, go to: https://nam03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=02%7C01%7Cmmodel%40KENT.EDU%7C7e0557a2214f442f00ce08d86b630690%7Ce5a06f4a1ec44d018f73e7dd15f26134%7C1%7C0%7C637377420338521101&sdata=7lM9R0eis7Ut1v%2BRxTK0m%2FwTcA570FkyOTUCaQBLEj8%3D&reserved=0 Post images on https://nam03.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=02%7C01%7Cmmodel%40KENT.EDU%7C7e0557a2214f442f00ce08d86b630690%7Ce5a06f4a1ec44d018f73e7dd15f26134%7C1%7C0%7C637377420338521101&sdata=hIJcnoo0EQZMva32%2B0QcReRXSayI527T0U8ng7du%2FC4%3D&reserved=0 and include the link in your posting. ***** Dear confocalists, We have several cameras that have accumulated dust on or near the sensor, and will appreciate any advice on how to get rid of those annoying specks that degrade images so much. We do not have a clean room in our building. Thanks in advance for your input. Javier -- Fco. Javier Diez-Guerra, PhD Servicio de Microscopía Confocal Centro de Biologia Molecular Severo Ochoa C/ Nicolás Cabrera, 1 Campus de Cantoblanco 28049 Madrid SPAIN Tel +34 91 196 4612 e-mail: [hidden email] |
Zdenek Svindrych-2 |
In reply to this post by F Javier Diez Guerra
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Javier, do you have a cell culture hood nearby? People sometimes use those to fix cameras and harddrives... Another important point is: Is the camera clean now? You can see the dust best in transmitted light image (brightfield, DIC, phase contrast), not so much in fluorescence, and if you detach the camera to clean it, you can't see the dust in the image at all (due to the large etendue of the room light coming to the camera chip). What I found very useful is a divergent point source of light in a dimmed room, few meters away from the camera (a small spot of a powerful laser pointer on the wall, laser pointer or LED flashlight with the front lens removed, etc). Then with the camera connected to the computer you can see right away how your cleaning attempts are successful. And it's quick, you don't need to disconnect the camera or carry it anywhere... Best, zdenek On Thu, Oct 8, 2020 at 4:20 AM F Javier Diez Guerra <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Dear confocalists, > > We have several cameras that have accumulated dust on or near the > sensor, and will appreciate any advice on how to get rid of those > annoying specks that degrade images so much. > > We do not have a clean room in our building. > > Thanks in advance for your input. > > Javier > > > -- > Fco. Javier Diez-Guerra, PhD > > Servicio de Microscopía Confocal > Centro de Biologia Molecular Severo Ochoa > C/ Nicolás Cabrera, 1 > Campus de Cantoblanco > 28049 Madrid > SPAIN > > Tel +34 91 196 4612 > e-mail: [hidden email] > -- -- Zdenek Svindrych, Ph.D. Research Scientist - Microscopy Imaging Specialist Department of Biochemistry and Cell Biology Geisel School of Medicine at Dartmouth |
Craig Brideau |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** As a first step I always try to verify the plane the dust is in. If you can unlock the camera and wiggle it, it is very easy to determine if the dust is on the camera or some other optical element in the system. Loosen the camera from the microscope and rotate it slightly while looking at the image on screen. If the dust moves as you move the camera, then it is not on the camera and is on some other optic. If it remains stationary as you move the camera then it is indeed on your camera itself. Craig On Thu, Oct 8, 2020 at 6:33 AM Zdenek Svindrych <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Javier, > > do you have a cell culture hood nearby? People sometimes use those to fix > cameras and harddrives... > > Another important point is: Is the camera clean now? You can see the dust > best in transmitted light image (brightfield, DIC, phase contrast), not so > much in fluorescence, and if you detach the camera to clean it, you can't > see the dust in the image at all (due to the large etendue of the room > light coming to the camera chip). > What I found very useful is a divergent point source of light in a dimmed > room, few meters away from the camera (a small spot of a powerful laser > pointer on the wall, laser pointer or LED flashlight with the front lens > removed, etc). Then with the camera connected to the computer you can see > right away how your cleaning attempts are successful. > And it's quick, you don't need to disconnect the camera or carry it > anywhere... > > Best, zdenek > > On Thu, Oct 8, 2020 at 4:20 AM F Javier Diez Guerra <[hidden email]> > wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > Post images on http://www.imgur.com and include the link in your > posting. > > ***** > > > > Dear confocalists, > > > > We have several cameras that have accumulated dust on or near the > > sensor, and will appreciate any advice on how to get rid of those > > annoying specks that degrade images so much. > > > > We do not have a clean room in our building. > > > > Thanks in advance for your input. > > > > Javier > > > > > > -- > > Fco. Javier Diez-Guerra, PhD > > > > Servicio de Microscopía Confocal > > Centro de Biologia Molecular Severo Ochoa > > C/ Nicolás Cabrera, 1 > > Campus de Cantoblanco > > 28049 Madrid > > SPAIN > > > > Tel +34 91 196 4612 > > e-mail: [hidden email] > > > > > -- > -- > Zdenek Svindrych, Ph.D. > Research Scientist - Microscopy Imaging Specialist > Department of Biochemistry and Cell Biology > Geisel School of Medicine at Dartmouth > |
Glen MacDonald |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** First Contact has been very effective at dust removal for me. Do be careful about letting it dry completely before attempting to peel off and avoid getting it on plastic components. Glen > On Oct 8, 2020, at 10:14 AM, Craig Brideau <[hidden email]> wrote: > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > As a first step I always try to verify the plane the dust is in. If you can > unlock the camera and wiggle it, it is very easy to determine if the dust > is on the camera or some other optical element in the system. Loosen the > camera from the microscope and rotate it slightly while looking at the > image on screen. If the dust moves as you move the camera, then it is not > on the camera and is on some other optic. If it remains stationary as you > move the camera then it is indeed on your camera itself. > > Craig > > On Thu, Oct 8, 2020 at 6:33 AM Zdenek Svindrych <[hidden email]> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> Post images on http://www.imgur.com and include the link in your posting. >> ***** >> >> Hi Javier, >> >> do you have a cell culture hood nearby? People sometimes use those to fix >> cameras and harddrives... >> >> Another important point is: Is the camera clean now? You can see the dust >> best in transmitted light image (brightfield, DIC, phase contrast), not so >> much in fluorescence, and if you detach the camera to clean it, you can't >> see the dust in the image at all (due to the large etendue of the room >> light coming to the camera chip). >> What I found very useful is a divergent point source of light in a dimmed >> room, few meters away from the camera (a small spot of a powerful laser >> pointer on the wall, laser pointer or LED flashlight with the front lens >> removed, etc). Then with the camera connected to the computer you can see >> right away how your cleaning attempts are successful. >> And it's quick, you don't need to disconnect the camera or carry it >> anywhere... >> >> Best, zdenek >> >> On Thu, Oct 8, 2020 at 4:20 AM F Javier Diez Guerra <[hidden email]> >> wrote: >> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> Post images on http://www.imgur.com and include the link in your >> posting. >>> ***** >>> >>> Dear confocalists, >>> >>> We have several cameras that have accumulated dust on or near the >>> sensor, and will appreciate any advice on how to get rid of those >>> annoying specks that degrade images so much. >>> >>> We do not have a clean room in our building. >>> >>> Thanks in advance for your input. >>> >>> Javier >>> >>> >>> -- >>> Fco. Javier Diez-Guerra, PhD >>> >>> Servicio de Microscopía Confocal >>> Centro de Biologia Molecular Severo Ochoa >>> C/ Nicolás Cabrera, 1 >>> Campus de Cantoblanco >>> 28049 Madrid >>> SPAIN >>> >>> Tel +34 91 196 4612 >>> e-mail: [hidden email] >>> >> >> >> -- >> -- >> Zdenek Svindrych, Ph.D. >> Research Scientist - Microscopy Imaging Specialist >> Department of Biochemistry and Cell Biology >> Geisel School of Medicine at Dartmouth >> |
Craig Brideau |
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** First Contact is good, but has a learning curve. Test a bit out on a slide or other piece of scrap glass before you try applying it to your optics. This will give you a feel for how thick a layer you should apply, how long you should let it dry, and how to go about peeling it off. Don't forget to embed a peeling tab, which also requires some tinkering to learn how to do correctly. Craig On Thu, Oct 8, 2020 at 11:55 AM Glen MacDonald <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > First Contact has been very effective at dust removal for me. Do be > careful about letting it dry completely before attempting to peel off and > avoid getting it on plastic components. > > Glen > > > On Oct 8, 2020, at 10:14 AM, Craig Brideau <[hidden email]> > wrote: > > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > Post images on http://www.imgur.com and include the link in your > posting. > > ***** > > > > As a first step I always try to verify the plane the dust is in. If you > can > > unlock the camera and wiggle it, it is very easy to determine if the dust > > is on the camera or some other optical element in the system. Loosen the > > camera from the microscope and rotate it slightly while looking at the > > image on screen. If the dust moves as you move the camera, then it is not > > on the camera and is on some other optic. If it remains stationary as you > > move the camera then it is indeed on your camera itself. > > > > Craig > > > > On Thu, Oct 8, 2020 at 6:33 AM Zdenek Svindrych <[hidden email]> > wrote: > > > >> ***** > >> To join, leave or search the confocal microscopy listserv, go to: > >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >> Post images on http://www.imgur.com and include the link in your > posting. > >> ***** > >> > >> Hi Javier, > >> > >> do you have a cell culture hood nearby? People sometimes use those to > fix > >> cameras and harddrives... > >> > >> Another important point is: Is the camera clean now? You can see the > dust > >> best in transmitted light image (brightfield, DIC, phase contrast), not > so > >> much in fluorescence, and if you detach the camera to clean it, you > can't > >> see the dust in the image at all (due to the large etendue of the room > >> light coming to the camera chip). > >> What I found very useful is a divergent point source of light in a > dimmed > >> room, few meters away from the camera (a small spot of a powerful laser > >> pointer on the wall, laser pointer or LED flashlight with the front lens > >> removed, etc). Then with the camera connected to the computer you can > see > >> right away how your cleaning attempts are successful. > >> And it's quick, you don't need to disconnect the camera or carry it > >> anywhere... > >> > >> Best, zdenek > >> > >> On Thu, Oct 8, 2020 at 4:20 AM F Javier Diez Guerra <[hidden email] > > > >> wrote: > >> > >>> ***** > >>> To join, leave or search the confocal microscopy listserv, go to: > >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >>> Post images on http://www.imgur.com and include the link in your > >> posting. > >>> ***** > >>> > >>> Dear confocalists, > >>> > >>> We have several cameras that have accumulated dust on or near the > >>> sensor, and will appreciate any advice on how to get rid of those > >>> annoying specks that degrade images so much. > >>> > >>> We do not have a clean room in our building. > >>> > >>> Thanks in advance for your input. > >>> > >>> Javier > >>> > >>> > >>> -- > >>> Fco. Javier Diez-Guerra, PhD > >>> > >>> Servicio de Microscopía Confocal > >>> Centro de Biologia Molecular Severo Ochoa > >>> C/ Nicolás Cabrera, 1 > >>> Campus de Cantoblanco > >>> 28049 Madrid > >>> SPAIN > >>> > >>> Tel +34 91 196 4612 > >>> e-mail: [hidden email] > >>> > >> > >> > >> -- > >> -- > >> Zdenek Svindrych, Ph.D. > >> Research Scientist - Microscopy Imaging Specialist > >> Department of Biochemistry and Cell Biology > >> Geisel School of Medicine at Dartmouth > >> > |
Sylvie Le Guyader |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear all This is the FAQ about cleaning CCD sensors from the First Contact website: Yes First Contact™ does clean detector windows and unprotected CCD boards effectively and completely. We have done it many times. However, the user must proceed with caution and knowledge about the CCD he/she is going to clean. CCD boards which are not covered by a detector window are very delicate and the user must understand issues regarding static charges and CCD’s if attempting to clean this type of CCD. If you are not familiar enough with CCD, it is possible to create static charges when removing the dried film, which can damage the CCD. (Our ESD-DF formula can be used to clean these CCDs) If you did not know this before reading it here, we do not recommend cleaning the CCD by yourself until you get some more instruction. Some CCD mounts are made of plastic. As always when using First Contact™, the user must be careful not to brush the First Contact™ solution onto the plastic mount. First Contact™ solution may adhere to some plastics too tightly to peel off or could even dissolve the material. This is usually not a consideration for high quality laboratory equipment. They have a link to an interesting article about how to clean CCD sensors with First contact: http://www.photoniccleaning.com/v/vspfiles/downloadables/Documentation/CCDCleaningSPIE2008.pdf How does one know if the CCD one wants to clean is covered by a protective glass or not? Has anyone tried the ESD-DF formula? Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader, PhD Live Cell Imaging Facility Manager Karolinska Institutet- Bionut Dpt Blickagången 16, Room 7362 (lab)/7840 (office) 14157 Huddinge, Sweden mobile: +46 (0) 73 733 5008 LCI website Follow our microscopy blog! -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Craig Brideau Sent: 08 October 2020 20:16 To: [hidden email] Subject: Re: Get rid of dust in camera sensor ***** To join, leave or search the confocal microscopy listserv, go to: https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C73255e8f604d43ffac2008d86bb637f7%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637377777672830120&sdata=Z4QEVhpRVtzH6WaIIepjrDNKlGrF%2B028mU7v0andBJg%3D&reserved=0 Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C73255e8f604d43ffac2008d86bb637f7%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637377777672830120&sdata=gDjYjOUftWB%2F4X%2BE8HvIIh8ieC43TSgEoHhEQP3fuFQ%3D&reserved=0 and include the link in your posting. ***** First Contact is good, but has a learning curve. Test a bit out on a slide or other piece of scrap glass before you try applying it to your optics. This will give you a feel for how thick a layer you should apply, how long you should let it dry, and how to go about peeling it off. Don't forget to embed a peeling tab, which also requires some tinkering to learn how to do correctly. Craig On Thu, Oct 8, 2020 at 11:55 AM Glen MacDonald <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists > .umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=02%7C01%7Cs > ylvie.le.guyader%40KI.SE%7C73255e8f604d43ffac2008d86bb637f7%7Cbff7eef1 > cf4b4f32be3da1dda043c05d%7C0%7C0%7C637377777672840118&sdata=RUcOFf > HrmMjCDbM8ILDsDdx%2BhixH4od13UW4Ve%2BA8UA%3D&reserved=0 > Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C73255e8f604d43ffac2008d86bb637f7%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637377777672840118&sdata=4WLfig%2FlGHNIse%2BYA2m9rtT5cMjF8MysL1gC8Ki0heA%3D&reserved=0 and include the link in your posting. > ***** > > First Contact has been very effective at dust removal for me. Do be > careful about letting it dry completely before attempting to peel off > and avoid getting it on plastic components. > > Glen > > > On Oct 8, 2020, at 10:14 AM, Craig Brideau <[hidden email]> > wrote: > > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flis > > ts.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=02%7C01 > > %7Csylvie.le.guyader%40KI.SE%7C73255e8f604d43ffac2008d86bb637f7%7Cbf > > f7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637377777672840118&sdat > > a=RUcOFfHrmMjCDbM8ILDsDdx%2BhixH4od13UW4Ve%2BA8UA%3D&reserved=0 > > Post images on > > https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww > > .imgur.com%2F&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C73255e8 > > f604d43ffac2008d86bb637f7%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0 > > %7C637377777672840118&sdata=4WLfig%2FlGHNIse%2BYA2m9rtT5cMjF8Mys > > L1gC8Ki0heA%3D&reserved=0 and include the link in your > posting. > > ***** > > > > As a first step I always try to verify the plane the dust is in. If > > you > can > > unlock the camera and wiggle it, it is very easy to determine if the > > dust is on the camera or some other optical element in the system. > > Loosen the camera from the microscope and rotate it slightly while > > looking at the image on screen. If the dust moves as you move the > > camera, then it is not on the camera and is on some other optic. If > > it remains stationary as you move the camera then it is indeed on your camera itself. > > > > Craig > > > > On Thu, Oct 8, 2020 at 6:33 AM Zdenek Svindrych <[hidden email]> > wrote: > > > >> ***** > >> To join, leave or search the confocal microscopy listserv, go to: > >> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fli > >> sts.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=02%7C > >> 01%7Csylvie.le.guyader%40KI.SE%7C73255e8f604d43ffac2008d86bb637f7%7 > >> Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637377777672840118& > >> sdata=RUcOFfHrmMjCDbM8ILDsDdx%2BhixH4od13UW4Ve%2BA8UA%3D&reserv > >> ed=0 Post images on > >> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fww > >> w.imgur.com%2F&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C73255 > >> e8f604d43ffac2008d86bb637f7%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0% > >> 7C0%7C637377777672840118&sdata=4WLfig%2FlGHNIse%2BYA2m9rtT5cMjF > >> 8MysL1gC8Ki0heA%3D&reserved=0 and include the link in your > posting. > >> ***** > >> > >> Hi Javier, > >> > >> do you have a cell culture hood nearby? People sometimes use those > >> to > fix > >> cameras and harddrives... > >> > >> Another important point is: Is the camera clean now? You can see > >> the > dust > >> best in transmitted light image (brightfield, DIC, phase contrast), > >> not > so > >> much in fluorescence, and if you detach the camera to clean it, you > can't > >> see the dust in the image at all (due to the large etendue of the > >> room light coming to the camera chip). > >> What I found very useful is a divergent point source of light in a > dimmed > >> room, few meters away from the camera (a small spot of a powerful > >> laser pointer on the wall, laser pointer or LED flashlight with the > >> front lens removed, etc). Then with the camera connected to the > >> computer you can > see > >> right away how your cleaning attempts are successful. > >> And it's quick, you don't need to disconnect the camera or carry it > >> anywhere... > >> > >> Best, zdenek > >> > >> On Thu, Oct 8, 2020 at 4:20 AM F Javier Diez Guerra > >> <[hidden email] > > > >> wrote: > >> > >>> ***** > >>> To join, leave or search the confocal microscopy listserv, go to: > >>> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fl > >>> ists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=02% > >>> 7C01%7Csylvie.le.guyader%40KI.SE%7C73255e8f604d43ffac2008d86bb637f > >>> 7%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637377777672840118& > >>> amp;sdata=RUcOFfHrmMjCDbM8ILDsDdx%2BhixH4od13UW4Ve%2BA8UA%3D&r > >>> eserved=0 Post images on > >>> https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fw > >>> ww.imgur.com%2F&data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C732 > >>> 55e8f604d43ffac2008d86bb637f7%7Cbff7eef1cf4b4f32be3da1dda043c05d%7 > >>> C0%7C0%7C637377777672840118&sdata=4WLfig%2FlGHNIse%2BYA2m9rtT5 > >>> cMjF8MysL1gC8Ki0heA%3D&reserved=0 and include the link in your > >> posting. > >>> ***** > >>> > >>> Dear confocalists, > >>> > >>> We have several cameras that have accumulated dust on or near the > >>> sensor, and will appreciate any advice on how to get rid of those > >>> annoying specks that degrade images so much. > >>> > >>> We do not have a clean room in our building. > >>> > >>> Thanks in advance for your input. > >>> > >>> Javier > >>> > >>> > >>> -- > >>> Fco. Javier Diez-Guerra, PhD > >>> > >>> Servicio de Microscopía Confocal > >>> Centro de Biologia Molecular Severo Ochoa C/ Nicolás Cabrera, 1 > >>> Campus de Cantoblanco > >>> 28049 Madrid > >>> SPAIN > >>> > >>> Tel +34 91 196 4612 > >>> e-mail: [hidden email] > >>> > >> > >> > >> -- > >> -- > >> Zdenek Svindrych, Ph.D. > >> Research Scientist - Microscopy Imaging Specialist Department of > >> Biochemistry and Cell Biology Geisel School of Medicine at > >> Dartmouth > >> > När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://ki.se/medarbetare/integritetsskyddspolicy>. Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://ki.se/en/staff/data-protection-policy>. |
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