Glass bottom 96 well plates for hi-res imaging

[Adrian Smith-6]

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Hi all,

We have a user who is after some 96well plates for hi-res imaging, ie coverslip thickness (glass?).

Any comments/recommendations would be appreciated.

Regards,

Adrian Smith
Centenary Institute, Sydney, Australia

[Eric Griffis]

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Hi Adrian,

I've had great success with plates from Matrical.  They sell #1.5 96-well plates with square wells, which maximises the number of cells you can image per well.  We found that they were more consistent than the Whatman plates and my cells grew much more happily in these plates.

Best regards,

Eric


Eric Griffis
Principal Investigator
Wellcome Trust Centre for Gene Regulation and Expression
College of Life Sciences
University of Dundee
MSI/WTB/JBC Complex
Dow Street
Dundee DD1 5EH
UK

Tel: [phone]
Fax: [phone]
Email: [hidden email]<mailto:[hidden email]>
Lab Website:  http://www.lifesci.dundee.ac.uk/groups/eric_griffis/GriffisLab/index.html
GRE Website: http://gre.lifesci.dundee.ac.uk



On 21 Jun 2012, at 10:02, Adrian Smith wrote:

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Hi all,

We have a user who is after some 96well plates for hi-res imaging, ie coverslip thickness (glass?).

Any comments/recommendations would be appreciated.

Regards,

Adrian Smith
Centenary Institute, Sydney, Australia



The University of Dundee is a registered Scottish Charity, No: SC015096

[Sylvie Le Guyader]

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Hi Adrian



We have 'recently' (several months) encountered great problems with the Matrical glass bottom 96-well plates. After using them for years without any trouble, the cells suddently started dying (all sorts of cells, any type of medium, in 2D or grown in a gel) as soon as they were seeded. Matrical claimed that they had not changed anything then they blamed the glue but the next batch of plates has the same problem.



Here are a few more companies (thin glass or thin plastic bottom):



MatTek<http://glass-bottom-dishes.com/pages/multiwellproducts.html>

Perkin Elmer<http://www.perkinelmer.com/SE/Catalog/Category/ID/Microplates>

BD<http://catalog.bd.com/bdCat/listProducts.doCustomer?categoryID=R39&priority=25&categoryName=Assay%20Plates%20and%20HTS%20Systems&categoryImgPath=/ecat/images/f44/family/plates.gif&sortByField=Product%20Number&viewPageNum=0>

Ibidi <http://www.ibidi.com/products/disposables/P_8962X_Plate96well.html>

Nexus<http://www.irori.com/pages/technology/keyAttributes.html>

Whatman<http://www.whatman.com/References/GlassBotClearViewMicroDataSheetFINAL3.26.10LR.pdf>



I strongly recommend you ask for a free sample to make sure the plates work for your cells and your imaging system.



Med vänlig hälsning / Best regards



Sylvie



@@@@@@@@@@@@@@@@@@@@@@@@

Sylvie Le Guyader

Live Cell Imaging Unit

Dept of Biosciences and Nutrition

Karolinska Institutet

Novum

14183 Huddinge

Sweden

office: [phone]

LCI room: [phone]

mobile: [phone]





> -----Original Message-----

> From: Confocal Microscopy List

> [mailto:[hidden email]] On Behalf Of Eric Griffis

> Sent: 21 June 2012 11:46

> To: [hidden email]

> Subject: Re: Glass bottom 96 well plates for hi-res imaging

>

> *****

> To join, leave or search the confocal microscopy listserv, go to:

> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy

> *****

>

> Hi Adrian,

>

> I've had great success with plates from Matrical.  They sell #1.5 96-well plates with

> square wells, which maximises the number of cells you can image per well.  We

> found that they were more consistent than the Whatman plates and my cells grew

> much more happily in these plates.

>

> Best regards,

>

> Eric

>

>

> Eric Griffis

> Principal Investigator

> Wellcome Trust Centre for Gene Regulation and Expression College of Life

> Sciences University of Dundee MSI/WTB/JBC Complex Dow Street Dundee DD1

> 5EH UK

>

> Tel: [phone]

> Fax: [phone]

> Email: [hidden email]<mailto:[hidden email]<mailto:[hidden email]%3cmailto:[hidden email]>>

> Lab Website:

> http://www.lifesci.dundee.ac.uk/groups/eric_griffis/GriffisLab/index.html

> GRE Website: http://gre.lifesci.dundee.ac.uk

>

>

>

> On 21 Jun 2012, at 10:02, Adrian Smith wrote:

>

> *****

> To join, leave or search the confocal microscopy listserv, go to:

> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy

> *****

>

> Hi all,

>

> We have a user who is after some 96well plates for hi-res imaging, ie coverslip

> thickness (glass?).

>

> Any comments/recommendations would be appreciated.

>

> Regards,

>

> Adrian Smith

> Centenary Institute, Sydney, Australia

>

>

>

> The University of Dundee is a registered Scottish Charity, No: SC015096

[simon walker (BI)-2]

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We had a similar problem with Povair plates.  The problem could be resolved with a methanol wash, though admittedly this isn't very practical with a large number of plates.
Simon

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Sylvie Le Guyader
Sent: 21 June 2012 13:22
To: [hidden email]
Subject: Re: Glass bottom 96 well plates for hi-res imaging

*****
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Hi Adrian



We have 'recently' (several months) encountered great problems with the Matrical glass bottom 96-well plates. After using them for years without any trouble, the cells suddently started dying (all sorts of cells, any type of medium, in 2D or grown in a gel) as soon as they were seeded. Matrical claimed that they had not changed anything then they blamed the glue but the next batch of plates has the same problem.



Here are a few more companies (thin glass or thin plastic bottom):



MatTek<http://glass-bottom-dishes.com/pages/multiwellproducts.html>

Perkin Elmer<http://www.perkinelmer.com/SE/Catalog/Category/ID/Microplates>

BD<http://catalog.bd.com/bdCat/listProducts.doCustomer?categoryID=R39&priority=25&categoryName=Assay%20Plates%20and%20HTS%20Systems&categoryImgPath=/ecat/images/f44/family/plates.gif&sortByField=Product%20Number&viewPageNum=0>

Ibidi <http://www.ibidi.com/products/disposables/P_8962X_Plate96well.html>

Nexus<http://www.irori.com/pages/technology/keyAttributes.html>

Whatman<http://www.whatman.com/References/GlassBotClearViewMicroDataSheetFINAL3.26.10LR.pdf>



I strongly recommend you ask for a free sample to make sure the plates work for your cells and your imaging system.



Med vänlig hälsning / Best regards



Sylvie



@@@@@@@@@@@@@@@@@@@@@@@@

Sylvie Le Guyader

Live Cell Imaging Unit

Dept of Biosciences and Nutrition

Karolinska Institutet

Novum

14183 Huddinge

Sweden

office: [phone]

LCI room: [phone]

mobile: [phone]





> -----Original Message-----

> From: Confocal Microscopy List

> [mailto:[hidden email]] On Behalf Of Eric Griffis

> Sent: 21 June 2012 11:46

> To: [hidden email]

> Subject: Re: Glass bottom 96 well plates for hi-res imaging

>

> *****

> To join, leave or search the confocal microscopy listserv, go to:

> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy

> *****

>

> Hi Adrian,

>

> I've had great success with plates from Matrical.  They sell #1.5 96-well plates with

> square wells, which maximises the number of cells you can image per well.  We

> found that they were more consistent than the Whatman plates and my cells grew

> much more happily in these plates.

>

> Best regards,

>

> Eric

>

>

> Eric Griffis

> Principal Investigator

> Wellcome Trust Centre for Gene Regulation and Expression College of Life

> Sciences University of Dundee MSI/WTB/JBC Complex Dow Street Dundee DD1

> 5EH UK

>

> Tel: [phone]

> Fax: [phone]

> Email: [hidden email]<mailto:[hidden email]<mailto:[hidden email]%3cmailto:[hidden email]>>

> Lab Website:

> http://www.lifesci.dundee.ac.uk/groups/eric_griffis/GriffisLab/index.html

> GRE Website: http://gre.lifesci.dundee.ac.uk

>

>

>

> On 21 Jun 2012, at 10:02, Adrian Smith wrote:

>

> *****

> To join, leave or search the confocal microscopy listserv, go to:

> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy

> *****

>

> Hi all,

>

> We have a user who is after some 96well plates for hi-res imaging, ie coverslip

> thickness (glass?).

>

> Any comments/recommendations would be appreciated.

>

> Regards,

>

> Adrian Smith

> Centenary Institute, Sydney, Australia

>

>

>

> The University of Dundee is a registered Scottish Charity, No: SC015096
The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT Registered Charity No. 1053902.
The information transmitted in this email is directed only to the addressee. If you received this in error, please contact the sender and delete this email from your system. The contents of this e-mail are the views of the sender and do not necessarily represent the views of the Babraham Institute. Full conditions at: www.babraham.ac.uk<http://www.babraham.ac.uk/email_disclaimer.html>

[Julio Vazquez]

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Our customers had good success with Greiner Bio-One coverglass bottom plates, although I was not able to find them on their online catalog in a recent search.

hhttp://us.gbo.com/bioscience/categories/

Their Polysterene plates Cat # 655083/655086 are also good.


I've had good recommendations for Ibidi plates and Willco wells plates. They are supposed to be good optically, and cells are reported to grow well on them:

http://www.biophysics.com/pages/products/IBIDI/ibidi.html

http://www.ibidi.de/products/index.html

http://www.willcowells.com/ 


Although not coverglass bottom, optical plates from BD and Nunc have also given us good results. They do work well with lenses such as 20x/0.8 PlanApo, but we did not use them with higher NA immersion lenses; BD used to have also glass bottom plates (#357311); I'm not sure they still do. The BD Thin Bottom series (#353219) are "optical grade" plastic 190 microns thick. BD claims they are suitable for use with high NA objectives. Assuming the refractive index is right, oil immersion lenses shoudl not care too much about a 20 micron difference in thickness.

Nunc Optiplate: Nunc Cat # 165306; Sigma-Aldrich Cat # P8866; Fisher Scientific catalog # 12-566-35 and 12-566-37

(Nunc plates now sold by Thermo-Fisher)

Falcon BD BioCoat: 96-well clear bottom and 384 well thin bottom, BD Cat # 353219 (Fisher cat # 08-772-225)

Falcon BD BioCoat 384-well: BD bioscience cat 354667 (collagen coated) and others

BD Falcon glass bottom plates: # 357311 (96-well) / 357312 (384-well)

These plates come with different coatings, and therefore may have different catalog numbers. Just make sure to read the full specs before ordering.



--
Julio Vazquez
Fred Hutchinson Cancer Research Center
Seattle, WA 98109-1024


http://www.fhcrc.org/



On Jun 21, 2012, at 2:02 AM, Adrian Smith wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi all,
>
> We have a user who is after some 96well plates for hi-res imaging, ie coverslip thickness (glass?).
>
> Any comments/recommendations would be appreciated.
>
> Regards,
>
> Adrian Smith
> Centenary Institute, Sydney, Australia

[Craig Brideau]

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Our lab has had some success with the Mattek brand 96 well
coverslip-bottomed plates.

Craig


On Thu, Jun 21, 2012 at 10:08 AM, Julio Vazquez <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Our customers had good success with Greiner Bio-One coverglass bottom
> plates, although I was not able to find them on their online catalog in a
> recent search.
>
> hhttp://us.gbo.com/bioscience/categories/
>
> Their Polysterene plates Cat # 655083/655086 are also good.
>
>
> I've had good recommendations for Ibidi plates and Willco wells plates.
> They are supposed to be good optically, and cells are reported to grow well
> on them:
>
> http://www.biophysics.com/pages/products/IBIDI/ibidi.html
>
> http://www.ibidi.de/products/index.html
>
> http://www.willcowells.com/
>
>
> Although not coverglass bottom, optical plates from BD and Nunc have also
> given us good results. They do work well with lenses such as 20x/0.8
> PlanApo, but we did not use them with higher NA immersion lenses; BD used
> to have also glass bottom plates (#357311); I'm not sure they still do. The
> BD Thin Bottom series (#353219) are "optical grade" plastic 190 microns
> thick. BD claims they are suitable for use with high NA objectives.
> Assuming the refractive index is right, oil immersion lenses shoudl not
> care too much about a 20 micron difference in thickness.
>
> Nunc Optiplate: Nunc Cat # 165306; Sigma-Aldrich Cat # P8866; Fisher
> Scientific catalog # 12-566-35 and 12-566-37
>
> (Nunc plates now sold by Thermo-Fisher)
>
> Falcon BD BioCoat: 96-well clear bottom and 384 well thin bottom, BD Cat #
> 353219 (Fisher cat # 08-772-225)
>
> Falcon BD BioCoat 384-well: BD bioscience cat 354667 (collagen coated) and
> others
>
> BD Falcon glass bottom plates: # 357311 (96-well) / 357312 (384-well)
>
> These plates come with different coatings, and therefore may have
> different catalog numbers. Just make sure to read the full specs before
> ordering.
>
>
>
> --
> Julio Vazquez
> Fred Hutchinson Cancer Research Center
> Seattle, WA 98109-1024
>
>
> http://www.fhcrc.org/
>
>
>
> On Jun 21, 2012, at 2:02 AM, Adrian Smith wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Hi all,
> >
> > We have a user who is after some 96well plates for hi-res imaging, ie
> coverslip thickness (glass?).
> >
> > Any comments/recommendations would be appreciated.
> >
> > Regards,
> >
> > Adrian Smith
> > Centenary Institute, Sydney, Australia
>

[G. Esteban Fernandez]

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I've never used them (nor do I have commercial interest) but you can
get a free sample from ibidi: http://ibidi.com/freesamples.  The
bottom is "coverslip-like #1.5" plastic.  I have used their chamber
slides with no problem.

-Esteban


On Thu, Jun 21, 2012 at 9:08 AM, Julio Vazquez <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Our customers had good success with Greiner Bio-One coverglass bottom plates, although I was not able to find them on their online catalog in a recent search.
>
> hhttp://us.gbo.com/bioscience/categories/
>
> Their Polysterene plates Cat # 655083/655086 are also good.
>
>
> I've had good recommendations for Ibidi plates and Willco wells plates. They are supposed to be good optically, and cells are reported to grow well on them:
>
> http://www.biophysics.com/pages/products/IBIDI/ibidi.html
>
> http://www.ibidi.de/products/index.html
>
> http://www.willcowells.com/
>
>
> Although not coverglass bottom, optical plates from BD and Nunc have also given us good results. They do work well with lenses such as 20x/0.8 PlanApo, but we did not use them with higher NA immersion lenses; BD used to have also glass bottom plates (#357311); I'm not sure they still do. The BD Thin Bottom series (#353219) are "optical grade" plastic 190 microns thick. BD claims they are suitable for use with high NA objectives. Assuming the refractive index is right, oil immersion lenses shoudl not care too much about a 20 micron difference in thickness.
>
> Nunc Optiplate: Nunc Cat # 165306; Sigma-Aldrich Cat # P8866; Fisher Scientific catalog # 12-566-35 and 12-566-37
>
> (Nunc plates now sold by Thermo-Fisher)
>
> Falcon BD BioCoat: 96-well clear bottom and 384 well thin bottom, BD Cat # 353219 (Fisher cat # 08-772-225)
>
> Falcon BD BioCoat 384-well: BD bioscience cat 354667 (collagen coated) and others
>
> BD Falcon glass bottom plates: # 357311 (96-well) / 357312 (384-well)
>
> These plates come with different coatings, and therefore may have different catalog numbers. Just make sure to read the full specs before ordering.
>
>
>
> --
> Julio Vazquez
> Fred Hutchinson Cancer Research Center
> Seattle, WA 98109-1024
>
>
> http://www.fhcrc.org/
>
>
>
> On Jun 21, 2012, at 2:02 AM, Adrian Smith wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Hi all,
>>
>> We have a user who is after some 96well plates for hi-res imaging, ie coverslip thickness (glass?).
>>
>> Any comments/recommendations would be appreciated.
>>
>> Regards,
>>
>> Adrian Smith
>> Centenary Institute, Sydney, Australia

[Jean-Pierre CLAMME-2]

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Hi Adrian,

One thing you may want to check is the position of the bottom of the wells
compare to the plastic frame. The greigner's one I had first were not
flush  with the frame and thus I had issues with the objective touching
the frame when imaging the wells on the edge. So I always had to sacrifice
the edge wells and decided to switch to the Matrical plates.

Best
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Jean-Pierre CLAMME, PhD
Chief Scientist
Nitto Denko Technical
501 Via Del Monte
Oceanside, CA 92058
E-mail: [hidden email]
Phone: [phone]


Confocal Microscopy List <[hidden email]> wrote on
06/21/2012 02:02:54 AM:

> Adrian Smith <[hidden email]>
> Sent by: Confocal Microscopy List <[hidden email]>
>
> 06/21/2012 02:05 AM
>
> Please respond to
> Confocal Microscopy List <[hidden email]>
>
> To
>
> [hidden email]
>
> cc
>
> Subject
>
> Glass bottom 96 well plates for hi-res imaging
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
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> *****

> Hi all,

> We have a user who is after some 96well plates for hi-res imaging,
> ie coverslip thickness (glass?).

> Any comments/recommendations would be appreciated.

> Regards,

> Adrian Smith
> Centenary Institute, Sydney, Australia