Gold Standard immersion oil
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi all, Just want to know what immersion oils people are using to ensure minimal background. I recently tried the Cargille FF, but because I'm using TIRF I saw an enormous change in the image due to the RI difference compared to regular immersion oils. So I'd like to stick to the usual 1.52 ish. I'm working at 488nm for your information Thanks for your help Neil Kad University of Essex |
 
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Craig Brideau |
Re: Gold Standard immersion oil
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I'm just using Cargille type A and B. 'A' for upright scopes and 'B' for inverted (because it's thicker). Apparently you can actually mix some of these oils together to get intermediate thickness and index values, so it may be possible to mix your own for an intermediate viscosity and index. I haven't tried this myself yet though.
Craig On Wed, Sep 3, 2008 at 9:56 AM, SUBSCRIBE CONFOCAL Neil M. Kad <[hidden email]> wrote: Search the CONFOCAL archive at |
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Rietdorf, Jens |
Re: Gold Standard immersion oil
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In reply to this post by Neil Kad
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear Neil, Recently, Olympus has released a new RI 1.52 immersion oil, which they claim to have tested against the oil Leica and Zeiss are using, and found to be superior (lower background) in the UV and otherwise similar. Colleagues have recently tested a cheaper oil of Merck which was performing less good than the Leica/Zeiss. No commercial interest in any of the above mentioned. Best regards, jens --- dr. jens rietdorf head microscopy novartis research foundation friedrich-miescher-institute, wro1066.2.16 maulbeerstr.66, 4058 basel, switzerland couriel:rietdorf(at)fmi(dot)ch fon: +41616975172 fax: +41616973976 -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of SUBSCRIBE CONFOCAL Neil M. Kad Sent: Wednesday, September 03, 2008 5:56 PM To: [hidden email] Subject: Gold Standard immersion oil Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi all, Just want to know what immersion oils people are using to ensure minimal background. I recently tried the Cargille FF, but because I'm using TIRF I saw an enormous change in the image due to the RI difference compared to regular immersion oils. So I'd like to stick to the usual 1.52 ish. I'm working at 488nm for your information Thanks for your help Neil Kad University of Essex |
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Barbara Foster |
Re: Gold Standard immersion oil
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In reply to this post by Neil Kad
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Hi, Craig
While mixing Type A and B will give you intermediatary thicknesses, it will have little change on RI: Type A and B are both 1.5150 for the commonly used sodium D line. However, your concept is correct. Cargille has several sets of test and calibration liquids, and, by mixing them proportionately, you can, indeed, achieve the intermediate refractive indices. This practice has long been used by chemical microscopists. For those of you interested in following through on this line of study, Cargille used to have a great little reference book by Dr. R. D. Allen on refractometry. They also now have some basic but good info on their website <a href="http://www.cargille.com). /" eudora="autourl">www.cargille.com). Hope this was helpful, Barbara Foster, President Microscopy/Microscopy Education 7101 Royal Glen Trail, Suite A McKinney TX 75070 P: (972)924-5310 Skype: fostermme W: www.MicroscopyEducation.com NEWS! Visit the NEW and IMPROVED www.MicroscopyEducation.com! And don't forget: MME is now scheduling customized, on-site courses through Dec 2008. Call me for a free assessment and quote. At 01:18 PM 9/3/2008, you wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal |
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Craig Brideau |
Re: Gold Standard immersion oil
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Thanks for the tip! I was looking on the Cargille website at the oil specs, and most of them are 1.52XX (including A and B) except for type FF, which is 1.48. So theoretically you could mix the FF with A or B and get somewhere between 1.52 and 1.48 depending on the ratios. Here's the table with the info:
http://www.cargille.com/immeroilspecs.shtml Craig On Wed, Sep 3, 2008 at 2:36 PM, Barbara Foster <[hidden email]> wrote:
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Donnelly, Tom |
Re: Gold Standard immersion oil
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In reply to this post by Neil Kad
I think there are problems mixing different series of Cargille oils. |
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Jason Swedlow |
Re: Gold Standard immersion oil
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In reply to this post by Craig Brideau
Search the CONFOCAL archive at
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Hi Craig-
I'm pretty sure you want to be careful mixing the different oils-- they are of different chemistry and might produce unpredictable results. Maybe call them and ask. However, we always used the Cargille Laser Liquids (http://www.cargille.com/laserliq.shtml) and the mixing trick there (I am pretty sure you have to mix w/w but again call) worked quite well. Most likely, you'll want the 5610 series. Regardless, note that all oils suffer dispersion and wavelength and temperature differences from that specified will cause them to have different effective RIs when, for example, used at 37 deg C. Cheers, Jason On Wed, Sep 3, 2008 at 10:25 PM, Craig Brideau <[hidden email]> wrote:
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Craig Brideau |
Re: Gold Standard immersion oil
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Search the CONFOCAL archive at
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That's a good point. They say the A and B will mix well, but they have almost identical refractive index values. The FF might not blend well with the A or B. Oil and Oil don't mix? @:-)
Craig On Wed, Sep 3, 2008 at 3:58 PM, Jason Swedlow <[hidden email]> wrote:
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi! I use the Zeiss Immersol 518F, but if you want Cargille, what about the DF version? No mixing involved. Remember to clean your objective before changing oil! Bye! Sophie ____________________________________________________ Sophie M. K. Brunet, Ph. D. Research Officer Optical Spectroscopy, Laser Systems and Applications Chemistry 112 sessional lecturer [hidden email] 306-966-1719 (office) 306-966-1702 (fax) ____________________________________________________ Saskatchewan Structural Sciences Centre University of Saskatchewan Thorvaldson Bldg. 110 Science Place Saskatoon, Sk S7N 5C9 ____________________________________________________ Quoting Craig Brideau <[hidden email]>: > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > That's a good point. They say the A and B will mix well, but they have > almost identical refractive index values. The FF might not blend well with > the A or B. Oil and Oil don't mix? @:-) > > Craig > > > On Wed, Sep 3, 2008 at 3:58 PM, Jason Swedlow > <[hidden email]>wrote: > > > Search the CONFOCAL archive at > > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > Hi Craig- > > > > I'm pretty sure you want to be careful mixing the different oils-- they are > > of different chemistry and might produce unpredictable results. Maybe call > > them and ask. However, we always used the Cargille Laser Liquids ( > > http://www.cargille.com/laserliq.shtml) and the mixing trick there (I am > > pretty sure you have to mix w/w but again call) worked quite well. Most > > likely, you'll want the 5610 series. Regardless, note that all oils suffer > > dispersion and wavelength and temperature differences from that specified > > will cause them to have different effective RIs when, for example, used at > > 37 deg C. > > > > Cheers, > > > > Jason > > > > > > On Wed, Sep 3, 2008 at 10:25 PM, Craig Brideau > <[hidden email]>wrote: > > > >> Search the CONFOCAL archive at > >> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >> Thanks for the tip! I was looking on the Cargille website at the oil > >> specs, and most of them are 1.52XX (including A and B) except for type FF, > >> which is 1.48. So theoretically you could mix the FF with A or B and get > >> somewhere between 1.52 and 1.48 depending on the ratios. Here's the table > >> with the info: > >> http://www.cargille.com/immeroilspecs.shtml > >> > >> Craig > >> > >> > >> > >> On Wed, Sep 3, 2008 at 2:36 PM, Barbara Foster <[hidden email]> wrote: > >> > >>> Search the CONFOCAL archive at > >>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >>> Hi, Craig > >>> > >>> While mixing Type A and B will give you intermediatary thicknesses, it > >>> will have little change on RI: Type A and B are both 1.5150 for the > >>> commonly used sodium D line. > >>> > >>> However, your concept is correct. Cargille has several sets of test and > >>> calibration liquids, and, by mixing them proportionately, you can, > indeed, > >>> achieve the intermediate refractive indices. This practice has long been > >>> used by chemical microscopists. > >>> > >>> For those of you interested in following through on this line of study, > >>> Cargille used to have a great little reference book by Dr. R. D. Allen on > >>> refractometry. They also now have some basic but good info on their > website > >>> www.cargille.com). <http://www.cargille.com%29.%C2%A0/> > >>> > >>> Hope this was helpful, > >>> *Barbara Foster, President > >>> > >>> Microscopy/Microscopy Education > >>> *7101 Royal Glen Trail, Suite A > >>> McKinney TX 75070 > >>> P: (972)924-5310 > >>> Skype: fostermme > >>> W: www.MicroscopyEducation.com > >>> > >>> <http://www.microscopyeducation.com/>*NEWS! Visit the NEW and IMPROVED > >>> www.MicroscopyEducation.com <http://www.microscopyeducation.com/>! And > >>> don't forget: MME is now scheduling customized, on-site courses through > Dec > >>> 2008. Call me for a free assessment and quote. > >>> > >>> > >>> > >>> > >>> > >>> *At 01:18 PM 9/3/2008, you wrote: > >>> > >>> Search the CONFOCAL archive at > >>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >>> I'm just using Cargille type A and B. 'A' for upright scopes and 'B' for > >>> inverted (because it's thicker). Apparently you can actually mix some of > >>> these oils together to get intermediate thickness and index values, so it > >>> may be possible to mix your own for an intermediate viscosity and index. > I > >>> haven't tried this myself yet though. > >>> > >>> Craig > >>> > >>> > >>> On Wed, Sep 3, 2008 at 9:56 AM, SUBSCRIBE CONFOCAL Neil M. Kad < > >>> [hidden email]> wrote: Search the CONFOCAL archive at > >>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >>> Hi all, > >>> Just want to know what immersion oils people are using to ensure minimal > >>> background. > >>> I recently tried the Cargille FF, but because I'm using TIRF I saw an > enormous > >>> change in the image due to the RI difference compared to regular > immersion > >>> oils. So I'd like to stick to the usual 1.52 ish. > >>> I'm working at 488nm for your information > >>> Thanks for your help > >>> Neil Kad University of Essex > >>> > >>> > >> > > > > > |
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In reply to this post by Neil Kad
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Thanks for your help. I've got some DF from Cargille on order now. If anyone else has suggestions for good low fluorescence oil, I'm still interested. Neil |
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Keep in mind that immersion oils do not only differ in refractive index but also in dispersive index. That means the same oil may work perfectly with the microscopes of one manufacturer and produce very noticeable chromatic aberrations with those from another. Beat At 12:08 05-09-2008, you wrote: >Search the CONFOCAL archive at >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >Thanks for your help. I've got some DF from Cargille on order now. If anyone >else has suggestions for good low fluorescence oil, I'm still interested. > >Neil |
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Martin Wessendorf-2 |
Re: Gold Standard immersion oil
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear Beat-- Beat Ludin wrote: > Keep in mind that immersion oils do not only differ in refractive index > but also in dispersive index. That means the same oil may work perfectly > with the microscopes of one manufacturer and produce very noticeable > chromatic aberrations with those from another. --I would like to try to illustrate this to a class. Do you know of any good examples of combinations that "don't work"? Thanks! Martin Wessendorf -- Martin Wessendorf, Ph.D. office: (612) 626-0145 Assoc Prof, Dept Neuroscience lab: (612) 624-2991 University of Minnesota Preferred FAX: (612) 624-8118 6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009 Minneapolis, MN 55455 **MY E-MAIL ADDRESS HAS CHANGED. PLEASE USE [hidden email] ** |
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