HBO/Mercury lamp lifetimes

[David Basiji]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Absolutely. I had 200W bulb blow in a Zeiss pumpkin.
Scared the sh*t out of me and everone else in the
lab at the time. Fortunately, nothing escaped the
housing but the inside was completely trashed.

-db


David Basiji, Ph.D.
President and CEO, Amnis Corporation
2505 Third Ave., Suite 210
Seattle, WA 98121
[phone] direct
[phone] mobile
[phone] fax
 
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-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Michael Cammer
Sent: Wednesday, May 07, 2008 10:44 AM
To: [hidden email]
Subject: Re: HBO/Mercury lamp lifetimes ***COMMERCIAL RESPONSE***

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Just out of curiosity, is there anyone on this list who has witnessed an
Hg lamp used for microscopy exploding?  Or is this a subject for an
article at http://snopes.com/ ?
________________________________________________________________________
____
Michael Cammer, Senior Light Microscopist, Analytical Imaging Facility,
Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY
10461
URLs: microscopy http://www.aecom.yu.edu/aif/ and art
http://coxcammer.com/
###########################################

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[Donnelly, Tom]

Yeah, but it was long ago; over 30 yrs
--------------------------
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----- Original Message -----
From: Confocal Microscopy List <[hidden email]>
To: [hidden email] <[hidden email]>
Sent: Wed May 07 10:44:09 2008
Subject: Re: HBO/Mercury lamp lifetimes ***COMMERCIAL RESPONSE***

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Just out of curiosity, is there anyone on this list who has witnessed
an Hg lamp used for microscopy exploding?  Or is this a subject for
an article at http://snopes.com/ ?
____________________________________________________________________________
Michael Cammer, Senior Light Microscopist, Analytical Imaging
Facility, Albert Einstein College of Medicine, 1300 Morris Park
Avenue, Bronx, NY  10461
URLs: microscopy http://www.aecom.yu.edu/aif/ and art http://coxcammer.com/

[Dale Callaham]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Michael,

I also had a 200W Hg lamp explode in a Zeiss "Pumpkin" housing. It was
powered by a Zeiss PS (metallic light green block with a meter). It
shattered the reflector and chipped the collector lens. I also saw the
results of an exploded 50W AC Hg lamp - again Zeiss, but it was not a
Zeiss issue: it was a departmental scope that everyone used and no one
knew about lamp care and life - no timer - the "hot potato" no one
wanted to have to buy and install the new lamp.

A very interesting thing I'm getting from a few of these messages (last
week Karen Lovley, and today someone else) is that some people with
Zeiss Attoarc supplies <<that are capable of it>> have been using the
100W Hg lamps at ~20% of rated power and getting over a thousand hours
of life and enough light to locate cells! Could save much life/ignitions
by idling at low power.

Dale Callaham


Michael Cammer wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Just out of curiosity, is there anyone on this list who has witnessed an
> Hg lamp used for microscopy exploding?  Or is this a subject for an
> article at http://snopes.com/ ?
> ____________________________________________________________________________
>
> Michael Cammer, Senior Light Microscopist, Analytical Imaging Facility,
> Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY  
> 10461
> URLs: microscopy http://www.aecom.yu.edu/aif/ and art http://coxcammer.com/

[Boisvert, Karen]

I was very close to the housing when our first one blew up - it sounds like a gun going off!  It blew up 30 seconds into the start up procedure.  There were 170 hours on the bulb itself and 5,000 hours on the power supply. The replacement bulb blew up with 71 hours on it.  Leica sent me a new power supply, new mercury bulb housing and a new mercury bulb.  They were great!
 
Karen
 
Karen Dalecki Boisvert
Division of Comparative Pathology
Confocal Microscopy Unit
Harvard University
TEL:   [phone]
FAX:  [phone]
email:  [hidden email]

        -----Original Message-----
        From: Confocal Microscopy List on behalf of Michael Cammer
        Sent: Wed 5/7/2008 1:44 PM
        To: [hidden email]
        Cc:
        Subject: Re: HBO/Mercury lamp lifetimes ***COMMERCIAL RESPONSE***
       
       

        Search the CONFOCAL archive at
        http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
       
        Just out of curiosity, is there anyone on this list who has witnessed
        an Hg lamp used for microscopy exploding?  Or is this a subject for
        an article at http://snopes.com/ ?
        ____________________________________________________________________________
        Michael Cammer, Senior Light Microscopist, Analytical Imaging
        Facility, Albert Einstein College of Medicine, 1300 Morris Park
        Avenue, Bronx, NY  10461
        URLs: microscopy http://www.aecom.yu.edu/aif/ and art http://coxcammer.com/
       

[J.P. Shields]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

About seven years ago we had a Hg lamp on our confocal blow.  I was in the
next room and had to usher out the student working on the scope, close the
door then wait a day before opening to start cleaning.
Fortunately, no damage to the housing or the student.
John Shields
Univ. of Georgia

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Michael Cammer
Sent: Wednesday, May 07, 2008 1:44 PM
To: [hidden email]
Subject: Re: HBO/Mercury lamp lifetimes ***COMMERCIAL RESPONSE***

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Just out of curiosity, is there anyone on this list who has witnessed
an Hg lamp used for microscopy exploding?  Or is this a subject for
an article at http://snopes.com/ ?
____________________________________________________________________________
Michael Cammer, Senior Light Microscopist, Analytical Imaging
Facility, Albert Einstein College of Medicine, 1300 Morris Park
Avenue, Bronx, NY  10461
URLs: microscopy http://www.aecom.yu.edu/aif/ and art http://coxcammer.com/

[Ed Monosov]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

never for 20 with something years

Michael Cammer wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Just out of curiosity, is there anyone on this list who has witnessed
> an Hg lamp used for microscopy exploding?  Or is this a subject for an
> article at http://snopes.com/ ?
> ____________________________________________________________________________
>
> Michael Cammer, Senior Light Microscopist, Analytical Imaging
> Facility, Albert Einstein College of Medicine, 1300 Morris Park
> Avenue, Bronx, NY  10461
> URLs: microscopy http://www.aecom.yu.edu/aif/ and art
> http://coxcammer.com/
>

--
        Edward Monosov, Ph.D.
        Director, Cell Imaging Core
        BURNHAM INSTITUTE for MEDICAL RESEARCH
        10901 N. Torrey Pines Rd, B5, R5146
        La Jolla, CA 92037
        P:[phone]; F:[phone]
        [hidden email]

[Mayandi Sivaguru]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
You can also try http://www.roithner-laser.at/
We have bought a couple from there and it worked.

At 10:11 AM 5/7/2008, you wrote:

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

for cheap mounted LEDs/drivers for microscopes you can try Thorlabs

http://www.thorlabs.com/NewGroupPage9.cfm?ObjectGroup_ID=2692&visNavID=551

I have no commercial interest here, they dont seem to have a 3-color system
yet but its a matter of time, maybe request it?

-----Original Message-----
From: Confocal Microscopy List [[hidden email]]On
Behalf Of Alison North
Sent: Wednesday, May 07, 2008 7:51 AM
To: [hidden email]
Subject: Re: HBO/Mercury lamp lifetimes


Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Jim,

Oh you cruel man, you are mercilessly TAUNTING those of us based in the
USA who would love to buy your LEDs but are prevented from doing so by
exceptionally irritating patent restrictions (hello to the company
concerned - your LED system is prohibitively expensive!). Or am I
missing something and this situation has recently changed?  Do keep us
posted please?  And yes, I am intentionally sending this to the
listserver, so that other people based in this country don't get all
excited by your LEDs and have their hopes dashed, like us.  Plus, if I
am incorrect about the patent situation and we can in fact obtain an
LED-based lamp here at a reasonable price (without having to assemble
everything ourselves), we would like to know.

Thanks and best wishes,
Alison


James Beacher wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> Commercial Interest
>
>
> Having followed this discussion, we can contain ourselves no
> longer.........*LEDs!*
>
>
>
> JIM Beacher
> CoolLED Ltd
>
>
>
>     -----Original Message-----
>     *From:* "Confocal Microscopy List" <[hidden email]>
>     *Sent:* 07/05/2008 14:50
>     *To:* "[hidden email]" <[hidden email]>
>     *Subject:* Re: HBO/Mercury lamp lifetimes
>
>
>     Search the CONFOCAL archive at
>     http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>     Hi all,
>
>     I would like to go back to an earlier post, and reiterate that there
>     are several different types of power supplies. The constant-current
>     ones increase the applied voltage as the "conductivity" of the bulb
>     decreases. This means that the amount of power deposited in the arc
>     slowly INCREASES as the electrodes age and eventually, this will
>     cause overheating, and softening of the fused-quartz envelope.
>     Constant-power supplies will not cause this problem, but the
>     intensity of the brightest part of the arc will slowly deteriorate
>     with time.
>
>     Another factor is dirt. Either finger grease or perhaps some
>     accidental event such as a small, fried insect or a very small piece
>     of flaked-off black paint. In short, anything that absorbs light and
>     gets hot while attached in some way to the quartz envelope. Any "dark
>     spot" will cause local overheating and asymmetrical envelope failure.
>
>     A second possibility has to to with the connections between the bulb
>     and the housing. If the mating surfaces of these metal parts become
>     corroded, or oxidized, the contact resistance will increase as will
>     the working temperature. This also can lead to premature envelope
>     failure.
>
>     Even the alignment of the spherical rear mirror can be important. If
>     the reflected image of the arc is formed not to the side of the arc
>     itself but concentrated onto a small section of the envelope, this
>     can exacerbate any asymmetrical heating cause by dirt or
>     contamination.
>
>     The effect of these "operator" factors will vary somewhat depending
>     on the type of power supply used.
>
>     Therefore, it seems to me that the list of important variables should
>     include not just be the bulb manufacturer, the bulb type  and the
>     design of the power supply but the care with which the new bulb has
>     been installed and aligned.
>
>     And then there is the whole matter of variable-power Hg systems. We
>     usually use our Atto-arc 50W at about 20% power and the bulbs often
>     seem to last for over a thousand hours. More than enough brightness
>     to find  the part of your sample best suited to confocal analysis.
>
>     Finally, there is the matter of xenon arcs. These have all the same
>     problems mentioned above, but in addition, because they are reputed
>     to start off having a much higher gas pressure, any resulting
>     explosions are said to be much more impressive, in fact even
>     dangerous (albeit, without the additional nuisance of Hg
>     contamination). Has anyone had any experience here?
>
>     Cheers,
>
>     Jim Pawley
>
>
>      >Search the CONFOCAL archive at
>      > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>      >
>      >Hello everyone
>      >
>      >I think we are barking under the wrong tree.  Please correct me if i
>      >am wrong, but many explosions I read reported in this thread (and
>      >only one -in 25 years- which happened to me @50hrs) happened at way
>      >less than the rated hours.
>      >
>      >Shouldn't  we revise the myth that changing the lamp early protects
>      >from explosions?
>      >
>      >It seems to me that it is either due to material failure (in which
>      >case I think the lamp manufacturers should pay the damage bill) or
>      >too many ignitions, in which case the manufacturers should include
>      >this count in the specs.
>      >
>      >Regards,
>      >
>      >*********************************
>      >Stamatis Pagakis Ph.D.
>      >Biological Imaging Unit
>      >Biomedical Research Foundation, Academy of Athens,  Greece
>      >
>      >>On 4 May 2008, at 00:54, Russell Spear wrote:
>      >>Search the CONFOCAL archive at
>      >> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>      >>
>      >>So it isn't worth the risk to use them past rated hours.
>      >>
>      >>Russ
>      >>
>      >>
>      >>
>      >>Russell N. Spear
>      >>Sr. Research Specialist
>      >>Dept. of Plant Pathology
>      >>Univ. of Wisconsin
>      >>1630 Linden Dr.
>      >>Madison WI 53706
>      >>
>      >>voice 608.263.2093
>      >>fax     [phone]
>
>
>     --
>                    **********************************************
>     Prof. James B. Pawley,                                      Ph.
>     608-263-3147
>     Room 223, Zoology Research Building,
>     FAX  [phone]
>     1117 Johnson Ave., Madison, WI, 53706
>     [hidden email]
>     3D Microscopy of Living Cells Course, June 14-26, 2008, UBC,
>     Vancouver Canada
>     Info: http://www.3dcourse.ubc.ca/           Applications due by
>     March 15, 2008
>                    "If it ain't diffraction, it must be statistics." Anon.
>

--
Alison J. North, Ph.D.,
Research Assistant Professor and
Director of the Bio-Imaging Resource Center,
The Rockefeller University,
1230 York Avenue,
New York,
NY 10065.
Tel: office     ++ 212 327 7488
Tel: lab        ++ 212 327 7486
Fax:            ++ 212 327 7489

Mayandi Sivaguru, PhD, PhD
Microscopy Facility Manager
8, Institute for Genomic Biology
University of Illinois at Urbana-Champaign
1206 West Gregory Dr.
Urbana, IL 61801 USA

Office: 217.333.1214
Fax: [phone]
[hidden email]
http://core.igb.uiuc.edu

[Barbara Foster]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi,

You can also try Fraen.  They make a wide variety of interesting LED illuminators. 
www.fraensrl.com/flmicro.html

B
At 04:45 PM 5/7/2008, you wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
You can also try http://www.roithner-laser.at/
We have bought a couple from there and it worked.

At 10:11 AM 5/7/2008, you wrote:

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

for cheap mounted LEDs/drivers for microscopes you can try Thorlabs

http://www.thorlabs.com/NewGroupPage9.cfm?ObjectGroup_ID=2692&visNavID=551

I have no commercial interest here, they dont seem to have a 3-color system
yet but its a matter of time, maybe request it?

-----Original Message-----
From: Confocal Microscopy List [ [hidden email]
Behalf Of Alison North
Sent: Wednesday, May 07, 2008 7:51 AM
To: [hidden email]
Subject: Re: HBO/Mercury lamp lifetimes


Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Jim,

Oh you cruel man, you are mercilessly TAUNTING those of us based in the
USA who would love to buy your LEDs but are prevented from doing so by
exceptionally irritating patent restrictions (hello to the company
concerned - your LED system is prohibitively expensive!). Or am I
missing something and this situation has recently changed?  Do keep us
posted please?  And yes, I am intentionally sending this to the
listserver, so that other people based in this country don't get all
excited by your LEDs and have their hopes dashed, like us.  Plus, if I
am incorrect about the patent situation and we can in fact obtain an
LED-based lamp here at a reasonable price (without having to assemble
everything ourselves), we would like to know.

Thanks and best wishes,
Alison


James Beacher wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> Commercial Interest
>
>
> Having followed this discussion, we can contain ourselves no
> longer.........*LEDs!*
>
>
>
> JIM Beacher
> CoolLED Ltd
>
>
>
>     -----Original Message-----
>     *From:* "Confocal Microscopy List" <[hidden email]>
>     *Sent:* 07/05/2008 14:50
>     *To:* "[hidden email]" <[hidden email]>
>     *Subject:* Re: HBO/Mercury lamp lifetimes
>
>
>     Search the CONFOCAL archive at
>     http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>     Hi all,
>
>     I would like to go back to an earlier post, and reiterate that there
>     are several different types of power supplies. The constant-current
>     ones increase the applied voltage as the "conductivity" of the bulb
>     decreases. This means that the amount of power deposited in the arc
>     slowly INCREASES as the electrodes age and eventually, this will
>     cause overheating, and softening of the fused-quartz envelope.
>     Constant-power supplies will not cause this problem, but the
>     intensity of the brightest part of the arc will slowly deteriorate
>     with time.
>
>     Another factor is dirt. Either finger grease or perhaps some
>     accidental event such as a small, fried insect or a very small piece
>     of flaked-off black paint. In short, anything that absorbs light and
>     gets hot while attached in some way to the quartz envelope. Any "dark
>     spot" will cause local overheating and asymmetrical envelope failure.
>
>     A second possibility has to to with the connections between the bulb
>     and the housing. If the mating surfaces of these metal parts become
>     corroded, or oxidized, the contact resistance will increase as will
>     the working temperature. This also can lead to premature envelope
>     failure.
>
>     Even the alignment of the spherical rear mirror can be important. If
>     the reflected image of the arc is formed not to the side of the arc
>     itself but concentrated onto a small section of the envelope, this
>     can exacerbate any asymmetrical heating cause by dirt or
>     contamination.
>
>     The effect of these "operator" factors will vary somewhat depending
>     on the type of power supply used.
>
>     Therefore, it seems to me that the list of important variables should
>     include not just be the bulb manufacturer, the bulb type  and the
>     design of the power supply but the care with which the new bulb has
>     been installed and aligned.
>
>     And then there is the whole matter of variable-power Hg systems. We
>     usually use our Atto-arc 50W at about 20% power and the bulbs often
>     seem to last for over a thousand hours. More than enough brightness
>     to find  the part of your sample best suited to confocal analysis.
>
>     Finally, there is the matter of xenon arcs. These have all the same
>     problems mentioned above, but in addition, because they are reputed
>     to start off having a much higher gas pressure, any resulting
>     explosions are said to be much more impressive, in fact even
>     dangerous (albeit, without the additional nuisance of Hg
>     contamination). Has anyone had any experience here?
>
>     Cheers,
>
>     Jim Pawley
>
>
>      >Search the CONFOCAL archive at
>      > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>      >
>      >Hello everyone
>      >
>      >I think we are barking under the wrong tree.  Please correct me if i
>      >am wrong, but many explosions I read reported in this thread (and
>      >only one -in 25 years- which happened to me @50hrs) happened at way
>      >less than the rated hours.
>      >
>      >Shouldn't  we revise the myth that changing the lamp early protects
>      >from explosions?
>      >
>      >It seems to me that it is either due to material failure (in which
>      >case I think the lamp manufacturers should pay the damage bill) or
>      >too many ignitions, in which case the manufacturers should include
>      >this count in the specs.
>      >
>      >Regards,
>      >
>      >*********************************
>      >Stamatis Pagakis Ph.D.
>      >Biological Imaging Unit
>      >Biomedical Research Foundation, Academy of Athens,  Greece
>      >
>      >>On 4 May 2008, at 00:54, Russell Spear wrote:
>      >>Search the CONFOCAL archive at
>      >> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>      >>
>      >>So it isn't worth the risk to use them past rated hours.
>      >>
>      >>Russ
>      >>
>      >>
>      >>
>      >>Russell N. Spear
>      >>Sr. Research Specialist
>      >>Dept. of Plant Pathology
>      >>Univ. of Wisconsin
>      >>1630 Linden Dr.
>      >>Madison WI 53706
>      >>
>      >>voice 608.263.2093
>      >>fax     [phone]
>
>
>     --
>                    **********************************************
>     Prof. James B. Pawley,                                      Ph.
>     608-263-3147
>     Room 223, Zoology Research Building,
>     FAX  [phone]
>     1117 Johnson Ave., Madison, WI, 53706
>     [hidden email]
>     3D Microscopy of Living Cells Course, June 14-26, 2008, UBC,
>     Vancouver Canada
>     Info: http://www.3dcourse.ubc.ca/          Applications due by
>     March 15, 2008
>                    "If it ain't diffraction, it must be statistics." Anon.
>

--
Alison J. North, Ph.D.,
Research Assistant Professor and
Director of the Bio-Imaging Resource Center,
The Rockefeller University,
1230 York Avenue,
New York,
NY 10065.
Tel: office     ++ 212 327 7488
Tel: lab        ++ 212 327 7486
Fax:            ++ 212 327 7489

Mayandi Sivaguru, PhD, PhD
Microscopy Facility Manager
8, Institute for Genomic Biology
University of Illinois at Urbana-Champaign
1206 West Gregory Dr.
Urbana, IL 61801 USA

Office: 217.333.1214
Fax: [phone]
[hidden email]
http://core.igb.uiuc.edu

[Craig Brideau]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal I've actually 'rolled my own' for a few different situations.  Just by a bag of LEDs from Thor, or Luxeon, or Philips, or whomever and wire them up yourself.  If you have the right dichroic mirrors you can do your own color mixing.  Remember that LEDs are very divergent light sources though; use a big lens to collimate their light efficiently.  I usually use a 2" plano-convex with a short focal length.  To drive them use a constant current source.  Thor sells a bunch of 'kit' current sources in their laser diode OEM section that work well enough for LEDs if you don't want to build your own from scratch with an LM317 or something.

Craig

On Wed, May 7, 2008 at 9:11 AM, Tim Murphy <[hidden email]> wrote:

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

for cheap mounted LEDs/drivers for microscopes you can try Thorlabs

http://www.thorlabs.com/NewGroupPage9.cfm?ObjectGroup_ID=2692&visNavID=551

I have no commercial interest here, they dont seem to have a 3-color system
yet but its a matter of time, maybe request it?

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]On
Behalf Of Alison North
Sent: Wednesday, May 07, 2008 7:51 AM
To: [hidden email]
Subject: Re: HBO/Mercury lamp lifetimes


Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Jim,

Oh you cruel man, you are mercilessly TAUNTING those of us based in the
USA who would love to buy your LEDs but are prevented from doing so by
exceptionally irritating patent restrictions (hello to the company
concerned - your LED system is prohibitively expensive!). Or am I
missing something and this situation has recently changed?  Do keep us
posted please?  And yes, I am intentionally sending this to the
listserver, so that other people based in this country don't get all
excited by your LEDs and have their hopes dashed, like us.  Plus, if I
am incorrect about the patent situation and we can in fact obtain an
LED-based lamp here at a reasonable price (without having to assemble
everything ourselves), we would like to know.

Thanks and best wishes,
Alison


James Beacher wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> Commercial Interest
>
>
> Having followed this discussion, we can contain ourselves no
> longer.........*LEDs!*
>
>
>
> JIM Beacher
> CoolLED Ltd
>
>
>
>     -----Original Message-----
>     *From:* "Confocal Microscopy List" <[hidden email]>
>     *Sent:* 07/05/2008 14:50
>     *To:* "[hidden email]" <[hidden email]>
>     *Subject:* Re: HBO/Mercury lamp lifetimes
>
>
>     Search the CONFOCAL archive at
>     http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>     Hi all,
>
>     I would like to go back to an earlier post, and reiterate that there
>     are several different types of power supplies. The constant-current
>     ones increase the applied voltage as the "conductivity" of the bulb
>     decreases. This means that the amount of power deposited in the arc
>     slowly INCREASES as the electrodes age and eventually, this will
>     cause overheating, and softening of the fused-quartz envelope.
>     Constant-power supplies will not cause this problem, but the
>     intensity of the brightest part of the arc will slowly deteriorate
>     with time.
>
>     Another factor is dirt. Either finger grease or perhaps some
>     accidental event such as a small, fried insect or a very small piece
>     of flaked-off black paint. In short, anything that absorbs light and
>     gets hot while attached in some way to the quartz envelope. Any "dark
>     spot" will cause local overheating and asymmetrical envelope failure.
>
>     A second possibility has to to with the connections between the bulb
>     and the housing. If the mating surfaces of these metal parts become
>     corroded, or oxidized, the contact resistance will increase as will
>     the working temperature. This also can lead to premature envelope
>     failure.
>
>     Even the alignment of the spherical rear mirror can be important. If
>     the reflected image of the arc is formed not to the side of the arc
>     itself but concentrated onto a small section of the envelope, this
>     can exacerbate any asymmetrical heating cause by dirt or
>     contamination.
>
>     The effect of these "operator" factors will vary somewhat depending
>     on the type of power supply used.
>
>     Therefore, it seems to me that the list of important variables should
>     include not just be the bulb manufacturer, the bulb type  and the
>     design of the power supply but the care with which the new bulb has
>     been installed and aligned.
>
>     And then there is the whole matter of variable-power Hg systems. We
>     usually use our Atto-arc 50W at about 20% power and the bulbs often
>     seem to last for over a thousand hours. More than enough brightness
>     to find  the part of your sample best suited to confocal analysis.
>
>     Finally, there is the matter of xenon arcs. These have all the same
>     problems mentioned above, but in addition, because they are reputed
>     to start off having a much higher gas pressure, any resulting
>     explosions are said to be much more impressive, in fact even
>     dangerous (albeit, without the additional nuisance of Hg
>     contamination). Has anyone had any experience here?
>
>     Cheers,
>
>     Jim Pawley
>
>
>      >Search the CONFOCAL archive at
>      >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>      >
>      >Hello everyone
>      >
>      >I think we are barking under the wrong tree.  Please correct me if i
>      >am wrong, but many explosions I read reported in this thread (and
>      >only one -in 25 years- which happened to me @50hrs) happened at way
>      >less than the rated hours.
>      >
>      >Shouldn't  we revise the myth that changing the lamp early protects
>      >from explosions?
>      >
>      >It seems to me that it is either due to material failure (in which
>      >case I think the lamp manufacturers should pay the damage bill) or
>      >too many ignitions, in which case the manufacturers should include
>      >this count in the specs.
>      >
>      >Regards,
>      >
>      >*********************************
>      >Stamatis Pagakis Ph.D.
>      >Biological Imaging Unit
>      >Biomedical Research Foundation, Academy of Athens,  Greece
>      >
>      >>On 4 May 2008, at 00:54, Russell Spear wrote:
>      >>Search the CONFOCAL archive at
>      >>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>      >>
>      >>So it isn't worth the risk to use them past rated hours.
>      >>
>      >>Russ
>      >>
>      >>
>      >>
>      >>Russell N. Spear
>      >>Sr. Research Specialist
>      >>Dept. of Plant Pathology
>      >>Univ. of Wisconsin
>      >>1630 Linden Dr.
>      >>Madison WI 53706
>      >>
>      >>voice 608.263.2093
>      >>fax     [phone]
>
>
>     --
>                    **********************************************
>     Prof. James B. Pawley,                                      Ph.
>     608-263-3147
>     Room 223, Zoology Research Building,
>     FAX  [phone]
>     1117 Johnson Ave., Madison, WI, 53706
>     [hidden email]
>     3D Microscopy of Living Cells Course, June 14-26, 2008, UBC,
>     Vancouver Canada
>     Info: http://www.3dcourse.ubc.ca/            Applications due by
>     March 15, 2008
>                    "If it ain't diffraction, it must be statistics." Anon.
>

--
Alison J. North, Ph.D.,
Research Assistant Professor and
Director of the Bio-Imaging Resource Center,
The Rockefeller University,
1230 York Avenue,
New York,
NY 10065.
Tel: office     ++ 212 327 7488
Tel: lab        ++ 212 327 7486
Fax:            ++ 212 327 7489

[Jim Beacher]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

 

Commercial Response

 

We would like to clarify the situation regarding of sales of our precisExcite LED light source in the USA.  We are currently not in a position to do so due to a patent issue.  We are in discussion with the owners of the patent and are seeking a resolution.  We will make an announcement as soon as we are in a position to do so.

 

We have a number of distribution agreements with microscope manufacturers and imaging software companies for sales worldwide.  At this time these agreements are similarly limited to exclude the USA.

 

We very much hope that this will be resolved soon.

 

Separately, we can advise that the flexibility of our LED system is now established with 11 discrete LED wavelengths available.  We will continue to add new wavelengths in the future.  LEDs will certainly address the problems discussed in this recent thread.

 

 

JIM Beacher

CoolLED Ltd.

[hidden email]

 

-----Original Message-----
From: "Confocal Microscopy List" <[hidden email]>
Sent: 07/05/2008 16:44
To: "[hidden email]" <[hidden email]>
Subject: Re: HBO/Mercury lamp lifetimes ***COMMERCIAL RESPONSE***

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi all,

Is this true?  Thanks for the response Gary but we had an e-mail from
Jim Beacher recently informing us that CoolLED could not currently
supply it to the USA.  Jim, could we have clarification on this issue
please?  Is it that only particular models are available here?

Thanks,
Alison

P.S.  Since an Andor person has taken the trouble to help me out,
I feel I should be allowed to comment on an Andor product at this point
- we finally got round to demo-ing the iXon 512 EMCCD on our spinning
disk yesterday and now I see what all the fuss is about - fabulous
camera guys!!!





Gary Laevsky wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi Alison and all,
>
> CoolLed has a 3 channel version available in the states for $8,500 list.
>
> Best,
>
> 
>
> Gary Laevsky, Ph.D.
>
> Imaging Application Specialist
>
> 
>
> Andor Technology
>
> discover new ways of seeing
>
> 
>
> [hidden email]
>
> Cell          (774) 291 - 9992
> Office       (860) 290 - 9211 x219
> Fax          (860) 290 - 9566
> Web:       www.andor.com
>
> -----Original Message-----
> From: Confocal Microscopy List [[hidden email]] On
> Behalf Of Alison North
> Sent: Wednesday, May 07, 2008 10:51 AM
> To: [hidden email]
> Subject: Re: HBO/Mercury lamp lifetimes
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi Jim,
>
> Oh you cruel man, you are mercilessly TAUNTING those of us based in the
> USA who would love to buy your LEDs but are prevented from doing so by
> exceptionally irritating patent restrictions (hello to the company
> concerned - your LED system is prohibitively expensive!). Or am I
> missing something and this situation has recently changed?  Do keep us
> posted please?  And yes, I am intentionally sending this to the
> listserver, so that other people based in this country don't get all
> excited by your LEDs and have their hopes dashed, like us.  Plus, if I
> am incorrect about the patent situation and we can in fact obtain an
> LED-based lamp here at a reasonable price (without having to assemble
> everything ourselves), we would like to know.
>
> Thanks and best wishes,
> Alison
>
>
> James Beacher wrote:
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>> Commercial Interest
>> 
>> 
>> Having followed this discussion, we can contain ourselves no
>> longer.........*LEDs!*
>> 
>> 
>> 
>> JIM Beacher
>> CoolLED Ltd
>> 
>> 
>>
>>     -----Original Message-----
>>     *From:* "Confocal Microscopy List" <[hidden email]>
>>     *Sent:* 07/05/2008 14:50
>>     *To:* "[hidden email]"
> <[hidden email]>
>>     *Subject:* Re: HBO/Mercury lamp lifetimes
>>
>>
>>     Search the CONFOCAL archive at
>>     http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>>     Hi all,
>>
>>     I would like to go back to an earlier post, and reiterate that
> there
>>     are several different types of power supplies. The
> constant-current
>>     ones increase the applied voltage as the "conductivity" of the
> bulb
>>     decreases. This means that the amount of power deposited in the
> arc
>>     slowly INCREASES as the electrodes age and eventually, this will
>>     cause overheating, and softening of the fused-quartz envelope.
>>     Constant-power supplies will not cause this problem, but the
>>     intensity of the brightest part of the arc will slowly deteriorate
>>     with time.
>>
>>     Another factor is dirt. Either finger grease or perhaps some
>>     accidental event such as a small, fried insect or a very small
> piece
>>     of flaked-off black paint. In short, anything that absorbs light
> and
>>     gets hot while attached in some way to the quartz envelope. Any
> "dark
>>     spot" will cause local overheating and asymmetrical envelope
> failure.
>>     A second possibility has to to with the connections between the
> bulb
>>     and the housing. If the mating surfaces of these metal parts
> become
>>     corroded, or oxidized, the contact resistance will increase as
> will
>>     the working temperature. This also can lead to premature envelope
>>     failure.
>>
>>     Even the alignment of the spherical rear mirror can be important.
> If
>>     the reflected image of the arc is formed not to the side of the
> arc
>>     itself but concentrated onto a small section of the envelope, this
>>     can exacerbate any asymmetrical heating cause by dirt or
>>     contamination.
>>
>>     The effect of these "operator" factors will vary somewhat
> depending
>>     on the type of power supply used.
>>
>>     Therefore, it seems to me that the list of important variables
> should
>>     include not just be the bulb manufacturer, the bulb type  and the
>>     design of the power supply but the care with which the new bulb
> has
>>     been installed and aligned.
>>
>>     And then there is the whole matter of variable-power Hg systems.
> We
>>     usually use our Atto-arc 50W at about 20% power and the bulbs
> often
>>     seem to last for over a thousand hours. More than enough
> brightness
>>     to find  the part of your sample best suited to confocal analysis.
>>
>>     Finally, there is the matter of xenon arcs. These have all the
> same
>>     problems mentioned above, but in addition, because they are
> reputed
>>     to start off having a much higher gas pressure, any resulting
>>     explosions are said to be much more impressive, in fact even
>>     dangerous (albeit, without the additional nuisance of Hg
>>     contamination). Has anyone had any experience here?
>>
>>     Cheers,
>>
>>     Jim Pawley
>>
>>
>>      >Search the CONFOCAL archive at
>>      >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>      >
>>      >Hello everyone
>>      >
>>      >I think we are barking under the wrong tree.  Please correct me
> if i
>>      >am wrong, but many explosions I read reported in this thread
> (and
>>      >only one -in 25 years- which happened to me @50hrs) happened at
> way
>>      >less than the rated hours.
>>      >
>>      >Shouldn't  we revise the myth that changing the lamp early
> protects
>>      >from explosions?
>>      >
>>      >It seems to me that it is either due to material failure (in
> which
>>      >case I think the lamp manufacturers should pay the damage bill)
> or
>>      >too many ignitions, in which case the manufacturers should
> include
>>      >this count in the specs.
>>      >
>>      >Regards,
>>      >
>>      >*********************************
>>      >Stamatis Pagakis Ph.D.
>>      >Biological Imaging Unit
>>      >Biomedical Research Foundation, Academy of Athens,  Greece
>>      >
>>      >>On 4 May 2008, at 00:54, Russell Spear wrote:
>>      >>Search the CONFOCAL archive at
>>      >>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>      >>
>>      >>So it isn't worth the risk to use them past rated hours.
>>      >>
>>      >>Russ
>>      >>
>>      >>
>>      >>
>>      >>Russell N. Spear
>>      >>Sr. Research Specialist
>>      >>Dept. of Plant Pathology
>>      >>Univ. of Wisconsin
>>      >>1630 Linden Dr.
>>      >>Madison WI 53706
>>      >>
>>      >>voice 608.263.2093
>>      >>fax     [phone]
>>
>>
>>     --
>>                    **********************************************
>>     Prof. James B. Pawley,                                      Ph.
>>     608-263-3147
>>     Room 223, Zoology Research Building,             
>>     FAX  [phone]
>>     1117 Johnson Ave., Madison, WI, 53706
>>     [hidden email]
>>     3D Microscopy of Living Cells Course, June 14-26, 2008, UBC,
>>     Vancouver Canada
>>     Info: http://www.3dcourse.ubc.ca/            Applications due by
>>     March 15, 2008
>>                    "If it ain't diffraction, it must be statistics."
> Anon.
>

--
Alison J. North, Ph.D.,
Research Assistant Professor and
Director of the Bio-Imaging Resource Center,
The Rockefeller University,
1230 York Avenue,
New York,
NY 10065.
Tel: office     ++ 212 327 7488
Tel: lab        ++ 212 327 7486
Fax:            ++ 212 327 7489

[Guy Cox]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

 I've had one explode - turned out that the power supply
was set to 60Hz rather than 50Hz - this seems to be deadly.
Only took out the mirror, though (replacement cost $15) so
it wasn't too expensive.  Never ever had one explode through
being used too long - they just get dimmer and dimmer.

                                              Guy



Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
    http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone [phone]     Fax [phone]
Mobile [phone]
______________________________________________
     http://www.guycox.net
-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ed Monosov
Sent: Thursday, 8 May 2008 6:43 AM
To: [hidden email]
Subject: Re: HBO/Mercury lamp lifetimes ***COMMERCIAL RESPONSE***

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

never for 20 with something years

Michael Cammer wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Just out of curiosity, is there anyone on this list who has witnessed
> an Hg lamp used for microscopy exploding?  Or is this a subject for an
> article at http://snopes.com/ ?
> ______________________________________________________________________
> ______
>
> Michael Cammer, Senior Light Microscopist, Analytical Imaging
> Facility, Albert Einstein College of Medicine, 1300 Morris Park
> Avenue, Bronx, NY  10461
> URLs: microscopy http://www.aecom.yu.edu/aif/ and art
> http://coxcammer.com/
>

--
        Edward Monosov, Ph.D.
        Director, Cell Imaging Core
        BURNHAM INSTITUTE for MEDICAL RESEARCH
        10901 N. Torrey Pines Rd, B5, R5146
        La Jolla, CA 92037
        P:[phone]; F:[phone]
        [hidden email]

No virus found in this incoming message.
Checked by AVG.
Version: 7.5.524 / Virus Database: 269.23.9/1418 - Release Date: 6/05/2008 5:17 PM
 

No virus found in this outgoing message.
Checked by AVG.
Version: 7.5.524 / Virus Database: 269.23.9/1418 - Release Date: 6/05/2008 5:17 PM
 

[Dale Callaham]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Is this Fraen product an epi-illumination system? It looks like the
illuminator mounts in the transmission path.

Dale

Barbara Foster wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi,
>
> You can also try Fraen.  They make a wide variety of interesting LED
> illuminators.
> www.fraensrl.com/flmicro.html
>
> <http://www.fraensrl.com/flmicro.html>B
> At 04:45 PM 5/7/2008, you wrote:
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>> You can also try http://www.roithner-laser.at/
>> We have bought a couple from there and it worked.
>>
>> At 10:11 AM 5/7/2008, you wrote:
>>> Search the CONFOCAL archive at
>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>>
>>> for cheap mounted LEDs/drivers for microscopes you can try Thorlabs
>>>
>>> http://www.thorlabs.com/NewGroupPage9.cfm?ObjectGroup_ID=2692&visNavID=551 
>>> <http://www.thorlabs.com/NewGroupPage9.cfm?ObjectGroup_ID=2692&visNavID=551>
>>>
>>>
>>> I have no commercial interest here, they dont seem to have a 3-color
>>> system
>>> yet but its a matter of time, maybe request it?
>>>
>>> -----Original Message-----
>>> From: Confocal Microscopy List [
>>> mailto:[hidden email]]On
>>> <mailto:[hidden email]%5DOn>
>>> Behalf Of Alison North
>>> Sent: Wednesday, May 07, 2008 7:51 AM
>>> To: [hidden email]
>>> Subject: Re: HBO/Mercury lamp lifetimes
>>>
>>>
>>> Search the CONFOCAL archive at
>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>>
>>> Hi Jim,
>>>
>>> Oh you cruel man, you are mercilessly TAUNTING those of us based in the
>>> USA who would love to buy your LEDs but are prevented from doing so by
>>> exceptionally irritating patent restrictions (hello to the company
>>> concerned - your LED system is prohibitively expensive!). Or am I
>>> missing something and this situation has recently changed?  Do keep us
>>> posted please?  And yes, I am intentionally sending this to the
>>> listserver, so that other people based in this country don't get all
>>> excited by your LEDs and have their hopes dashed, like us.  Plus, if I
>>> am incorrect about the patent situation and we can in fact obtain an
>>> LED-based lamp here at a reasonable price (without having to assemble
>>> everything ourselves), we would like to know.
>>>
>>> Thanks and best wishes,
>>> Alison
>>>
>>>
>>> James Beacher wrote:
>>> > Search the CONFOCAL archive at
>>> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>> > Commercial Interest
>>> >
>>> >
>>> > Having followed this discussion, we can contain ourselves no
>>> > longer.........*LEDs!*
>>> >
>>> >
>>> >
>>> > JIM Beacher
>>> > CoolLED Ltd
>>> >
>>> >
>>> >
>>> >     -----Original Message-----
>>> >     *From:* "Confocal Microscopy List" <[hidden email]>
>>> >     *Sent:* 07/05/2008 14:50
>>> >     *To:* "[hidden email]"
>>> <[hidden email]>
>>> >     *Subject:* Re: HBO/Mercury lamp lifetimes
>>> >
>>> >
>>> >     Search the CONFOCAL archive at
>>> >     http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>> >
>>> >     Hi all,
>>> >
>>> >     I would like to go back to an earlier post, and reiterate that
>>> there
>>> >     are several different types of power supplies. The constant-current
>>> >     ones increase the applied voltage as the "conductivity" of the bulb
>>> >     decreases. This means that the amount of power deposited in the arc
>>> >     slowly INCREASES as the electrodes age and eventually, this will
>>> >     cause overheating, and softening of the fused-quartz envelope.
>>> >     Constant-power supplies will not cause this problem, but the
>>> >     intensity of the brightest part of the arc will slowly deteriorate
>>> >     with time.
>>> >
>>> >     Another factor is dirt. Either finger grease or perhaps some
>>> >     accidental event such as a small, fried insect or a very small
>>> piece
>>> >     of flaked-off black paint. In short, anything that absorbs
>>> light and
>>> >     gets hot while attached in some way to the quartz envelope. Any
>>> "dark
>>> >     spot" will cause local overheating and asymmetrical envelope
>>> failure.
>>> >
>>> >     A second possibility has to to with the connections between the
>>> bulb
>>> >     and the housing. If the mating surfaces of these metal parts become
>>> >     corroded, or oxidized, the contact resistance will increase as will
>>> >     the working temperature. This also can lead to premature envelope
>>> >     failure.
>>> >
>>> >     Even the alignment of the spherical rear mirror can be
>>> important. If
>>> >     the reflected image of the arc is formed not to the side of the arc
>>> >     itself but concentrated onto a small section of the envelope, this
>>> >     can exacerbate any asymmetrical heating cause by dirt or
>>> >     contamination.
>>> >
>>> >     The effect of these "operator" factors will vary somewhat depending
>>> >     on the type of power supply used.
>>> >
>>> >     Therefore, it seems to me that the list of important variables
>>> should
>>> >     include not just be the bulb manufacturer, the bulb type  and the
>>> >     design of the power supply but the care with which the new bulb has
>>> >     been installed and aligned.
>>> >
>>> >     And then there is the whole matter of variable-power Hg systems. We
>>> >     usually use our Atto-arc 50W at about 20% power and the bulbs often
>>> >     seem to last for over a thousand hours. More than enough brightness
>>> >     to find  the part of your sample best suited to confocal analysis.
>>> >
>>> >     Finally, there is the matter of xenon arcs. These have all the same
>>> >     problems mentioned above, but in addition, because they are reputed
>>> >     to start off having a much higher gas pressure, any resulting
>>> >     explosions are said to be much more impressive, in fact even
>>> >     dangerous (albeit, without the additional nuisance of Hg
>>> >     contamination). Has anyone had any experience here?
>>> >
>>> >     Cheers,
>>> >
>>> >     Jim Pawley
>>> >
>>> >
>>> >      >Search the CONFOCAL archive at
>>> >      > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>> >      >
>>> >      >Hello everyone
>>> >      >
>>> >      >I think we are barking under the wrong tree.  Please correct
>>> me if i
>>> >      >am wrong, but many explosions I read reported in this thread (and
>>> >      >only one -in 25 years- which happened to me @50hrs) happened
>>> at way
>>> >      >less than the rated hours.
>>> >      >
>>> >      >Shouldn't  we revise the myth that changing the lamp early
>>> protects
>>> >      >from explosions?
>>> >      >
>>> >      >It seems to me that it is either due to material failure (in
>>> which
>>> >      >case I think the lamp manufacturers should pay the damage
>>> bill) or
>>> >      >too many ignitions, in which case the manufacturers should
>>> include
>>> >      >this count in the specs.
>>> >      >
>>> >      >Regards,
>>> >      >
>>> >      >*********************************
>>> >      >Stamatis Pagakis Ph.D.
>>> >      >Biological Imaging Unit
>>> >      >Biomedical Research Foundation, Academy of Athens,  Greece
>>> >      >
>>> >      >>On 4 May 2008, at 00:54, Russell Spear wrote:
>>> >      >>Search the CONFOCAL archive at
>>> >      >> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>> >      >>
>>> >      >>So it isn't worth the risk to use them past rated hours.
>>> >      >>
>>> >      >>Russ
>>> >      >>
>>> >      >>
>>> >      >>
>>> >      >>Russell N. Spear
>>> >      >>Sr. Research Specialist
>>> >      >>Dept. of Plant Pathology
>>> >      >>Univ. of Wisconsin
>>> >      >>1630 Linden Dr.
>>> >      >>Madison WI 53706
>>> >      >>
>>> >      >>voice 608.263.2093
>>> >      >>fax     [phone]
>>> >
>>> >
>>> >     --
>>> >                    **********************************************
>>> >     Prof. James B. Pawley,                                      Ph.
>>> >     608-263-3147
>>> >     Room 223, Zoology Research Building,
>>> >     FAX  [phone]
>>> >     1117 Johnson Ave., Madison, WI, 53706
>>> >     [hidden email]
>>> >     3D Microscopy of Living Cells Course, June 14-26, 2008, UBC,
>>> >     Vancouver Canada
>>> >     Info: http://www.3dcourse.ubc.ca/         
>>> <http://www.3dcourse.ubc.ca/%A0%A0%A0%A0%A0%A0%A0%A0%A0%A0>
>>> Applications due by
>>> >     March 15, 2008
>>> >                    "If it ain't diffraction, it must be
>>> statistics." Anon.
>>> >
>>>
>>> --
>>> Alison J. North, Ph.D.,
>>> Research Assistant Professor and
>>> Director of the Bio-Imaging Resource Center,
>>> The Rockefeller University,
>>> 1230 York Avenue,
>>> New York,
>>> NY 10065.
>>> Tel: office     ++ 212 327 7488
>>> Tel: lab        ++ 212 327 7486
>>> Fax:            ++ 212 327 7489
>>
>> Mayandi Sivaguru, PhD, PhD
>> Microscopy Facility Manager
>> 8, Institute for Genomic Biology
>> University of Illinois at Urbana-Champaign
>> 1206 West Gregory Dr.
>> Urbana, IL 61801 USA
>>
>> Office: 217.333.1214
>> Fax: [phone]
>> [hidden email]
>> http://core.igb.uiuc.edu <http://core.igb.uiuc.edu/>