Hands-on Confocal Course April 2010
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O'Toole, P |
Hands-on Confocal Course April 2010
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CONFOCAL MICROSCOPY
4-Day Intensive Hands-On Course Website: www.york.ac.uk/depts/biol/tf/imaging_course.htm Basics, Live-cell imaging, FRET, FRAP and Spectral Unmixing 20-23 April 2010 Technology Facility, Dept. Biology University of York, UK Instructors will include Peter O'Toole, University of York Jo Marrison, University of York Les Borland, Carl Zeiss Colin Hornby, Perkin Elmer and special guest lecture and practical session with Daniel Zicha (CRUK). Tutorials and practical sessions will cover both basic and advanced confocal microscopy. The course will enable participants to realise the potential behind many developing confocal techniques and realise the simplicity of applying the techniques to a broad range of sample types and fluorescent labels. Both novice and more experienced users welcome. Preliminary Programme DAY AIMS Day 1: Basics, Familiarisation, x,y,z and multicolour imaging. Day 2: Live-cell imaging with spinning disk confocal microscopy, FRAP on laser scanning confocal. Day 3: Advanced Techniques covering spectral unmixing and FRET with fluorescent proteins. Day 4: Further advances. More samples to explore confocal limits, and time for participants own samples. Thursday evening will include a special guest speaker and course meal. The course will utilise many different sample types ranging from cultured monolayers to plant cells. A diverse range of fluorescent labels will also be used, which will include various fluorescent proteins (CFP, GFP and YFP) and classic antibody labels. Over the four days, at least four confocal microscopes (2x Zeiss LSM 710 and 2x LSM 510 META on both an invert and upright microscopes, PerkinElmer UltraVIEW LCI or VoX) will be utilised. Accommodation (en-suite), breakfast and lunches will be provided for the length of the course. Evening meals will be provided on both Tuesday and Thursday evenings. Places are limited to 12 participants to permit full hands-on practice, so please book early to avoid disappointment. For further information, please visit the course website at: www.york.ac.uk/depts/biol/tf/imaging-cytometry/imaging_course.htm or contact Margaret Newton 01904 328821, [hidden email] -- Dr Peter O'Toole Head of Imaging and Cytometry Technology Facility Department of Biology (Area 15) University of York PO Box 373 YORK YO10 5YW Tel : +44 (0)1904 328722 Fax : +44 (0)1904 328804 email : [hidden email] www.york.ac.uk/biology/tf |
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Patrizia Ferretti |
Re: Hands-on Confocal Course April 2010
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Hi Bertrand,
We need to get the Image J course on the map before somebody else thinks of it! Best, Patrizia >CONFOCAL MICROSCOPY > >4-Day Intensive Hands-On Course > >Website: www.york.ac.uk/depts/biol/tf/imaging_course.htm > >Basics, Live-cell imaging, FRET, FRAP and Spectral Unmixing > >20-23 April 2010 >Technology Facility, Dept. Biology >University of York, UK > >Instructors will include > >Peter O'Toole, University of York >Jo Marrison, University of York >Les Borland, Carl Zeiss >Colin Hornby, Perkin Elmer >and special guest lecture and practical session with Daniel Zicha (CRUK). > >Tutorials and practical sessions will cover both basic and advanced >confocal microscopy. The course will enable participants to realise >the potential behind many developing confocal techniques and realise >the simplicity of applying the techniques to a broad range of sample >types and fluorescent labels. Both novice and more experienced users >welcome. > >Preliminary Programme >DAY AIMS >Day 1: Basics, Familiarisation, x,y,z and multicolour imaging. >Day 2: Live-cell imaging with spinning disk confocal microscopy, >FRAP on laser scanning confocal. >Day 3: Advanced Techniques covering spectral unmixing and FRET with >fluorescent proteins. >Day 4: Further advances. More samples to explore confocal limits, >and time for participants own samples. > >Thursday evening will include a special guest speaker and course meal. > >The course will utilise many different sample types ranging from >cultured monolayers to plant cells. A diverse range of fluorescent >labels will also be used, which will include various fluorescent >proteins (CFP, GFP and YFP) and classic antibody labels. Over the >four days, at least four confocal microscopes (2x Zeiss LSM 710 and >2x LSM 510 META on both an invert and upright microscopes, >PerkinElmer UltraVIEW LCI or VoX) will be utilised. > >Accommodation (en-suite), breakfast and lunches will be provided for >the length of the course. Evening meals will be provided on both >Tuesday and Thursday evenings. > >Places are limited to 12 participants to permit full hands-on >practice, so please book early to avoid disappointment. >For further information, please visit the course website at: >www.york.ac.uk/depts/biol/tf/imaging-cytometry/imaging_course.htm > >or contact Margaret Newton 01904 328821, [hidden email] > >-- >Dr Peter O'Toole >Head of Imaging and Cytometry >Technology Facility >Department of Biology (Area 15) >University of York >PO Box 373 >YORK >YO10 5YW > >Tel : +44 (0)1904 328722 >Fax : +44 (0)1904 328804 >email : [hidden email] >www.york.ac.uk/biology/tf -- _______________________________________________________ Dr Patrizia Ferretti Developmental Biology Unit UCL Institute of Child Health 30 Guilford Street London WC1N 1EH, UK Tel: (+44) 020-7829 8894 (direct line) (+44) 020 7905 2715 (secretary) (+44) 020 7242 9789 ICH switchboard Fax: (+44) 020 7831 4366 E-mail: [hidden email] http://www.ich.ucl.ac.uk/ich/academicunits/Developmental_biology/Homepage ________________________________________________________ |
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Tim O'Brien Sr. |
Carolina Workshop on Force Measurements and Manipulation in Biological Microscopy
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In reply to this post by O'Toole, P
We would like to let everyone know about our Sixth Annual Forces in Biology workshop. It is back at its old time, mid-May, which is a great time to be in Chapel Hill. The workshop is hosted by CISMM, our NIH resource (Computer Integrated Systems for Microscopy and Manipulation; CISMM.org). It consists of morning lectures followed by hands on force experiments on live specimens. The morning discussions provide a framework for understanding and analyzing forces on the micro and nanoscale, and serve as an introduction to the afternoon’s experiments. The afternoon sessions are hands-on laboratories where you perform force measurements such as fibrin fibers and perform biofluid rheology. Experiments use laser tweezers, an integrated atomic force microscopy/optical microscopy system, and 3D magnetic systems integrated with a fluorescence confocal microscope. We also include an introduction to microfluidics. Finally, participants spend a half day learning to use some of the many free software packages available from our resource that facilitate the analysis and visualization of 3D images and forces in biological systems (free analysis and visualization software is always available at CISMM.org). Registration is limited to 18, so you have lots of hands-on time with the microscopes and instructors. The $775 fee includes all supplies, light breakfasts, snacks and a conference dinner. For more information go to http://cismm.cs.unc.edu/resources/events Hope to see you there! |
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Nuno Moreno |
Re: Hands-on Confocal Course April 2010
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In reply to this post by Patrizia Ferretti
Hi!
I'm doing that for 5/6 six years ;) Ecclesiastes 1:9 Nuno Moreno, PhD ___________ Equipment Management Unit, Head Instituto Gulbenkian de Ciência Fundação Calouste Gulbenkian phone +351 214464606 fax +351 214407970 On 20-01-2010 15:33, Patrizia Ferretti wrote: > Hi Bertrand, > > We need to get the Image J course on the map before somebody else thinks > of it! > > Best, > > Patrizia > >> CONFOCAL MICROSCOPY >> >> 4-Day Intensive Hands-On Course >> >> Website: www.york.ac.uk/depts/biol/tf/imaging_course.htm >> >> Basics, Live-cell imaging, FRET, FRAP and Spectral Unmixing >> >> 20-23 April 2010 >> Technology Facility, Dept. Biology >> University of York, UK >> >> Instructors will include >> >> Peter O'Toole, University of York >> Jo Marrison, University of York >> Les Borland, Carl Zeiss >> Colin Hornby, Perkin Elmer >> and special guest lecture and practical session with Daniel Zicha (CRUK). >> >> Tutorials and practical sessions will cover both basic and advanced >> confocal microscopy. The course will enable participants to realise >> the potential behind many developing confocal techniques and realise >> the simplicity of applying the techniques to a broad range of sample >> types and fluorescent labels. Both novice and more experienced users >> welcome. >> >> Preliminary Programme >> DAY AIMS >> Day 1: Basics, Familiarisation, x,y,z and multicolour imaging. >> Day 2: Live-cell imaging with spinning disk confocal microscopy, FRAP >> on laser scanning confocal. >> Day 3: Advanced Techniques covering spectral unmixing and FRET with >> fluorescent proteins. >> Day 4: Further advances. More samples to explore confocal limits, and >> time for participants own samples. >> >> Thursday evening will include a special guest speaker and course meal. >> >> The course will utilise many different sample types ranging from >> cultured monolayers to plant cells. A diverse range of fluorescent >> labels will also be used, which will include various fluorescent >> proteins (CFP, GFP and YFP) and classic antibody labels. Over the four >> days, at least four confocal microscopes (2x Zeiss LSM 710 and 2x LSM >> 510 META on both an invert and upright microscopes, PerkinElmer >> UltraVIEW LCI or VoX) will be utilised. >> >> Accommodation (en-suite), breakfast and lunches will be provided for >> the length of the course. Evening meals will be provided on both >> Tuesday and Thursday evenings. >> >> Places are limited to 12 participants to permit full hands-on >> practice, so please book early to avoid disappointment. >> For further information, please visit the course website at: >> www.york.ac.uk/depts/biol/tf/imaging-cytometry/imaging_course.htm >> >> or contact Margaret Newton 01904 328821, [hidden email] >> >> -- >> Dr Peter O'Toole >> Head of Imaging and Cytometry >> Technology Facility >> Department of Biology (Area 15) >> University of York >> PO Box 373 >> YORK >> YO10 5YW >> >> Tel : +44 (0)1904 328722 >> Fax : +44 (0)1904 328804 >> email : [hidden email] >> www.york.ac.uk/biology/tf > > |
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John Runions |
Re: Hands-on Confocal Course April 2010
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In reply to this post by Patrizia Ferretti
Patrizia Ferretti wrote: Hi Bertrand, --
(Sent from my cra%#y
non-Blackberry electronic device that still has wires) *********************************
Visit
The Illuminated Plant
Cell
dot com |
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JOEL B. SHEFFIELD |
Re: Hands-on Confocal Course April 2010
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In reply to this post by O'Toole, P
I normally spend 6-8 weeks of my microscopy course on digital
imaging, based primarily on ImageJ. If you look at the ImageJ site, you will find several tutorials on the subject. Check: http://rsb.info.nih.gov/ij/docs/examples/index.html Among the resources is an introductory powerpoint that I presented at the M&M meeting in 2008: http://rsb.info.nih.gov/ij/docs/examples/IJ-M&M08.ppt --of couse, in the past two years, there have been many improvements, but the basics remain. Joel -------------- Original message --------------- Hey, I've just had a great idea - what about a course that teaches the basics of ImageJ? Patrizia Ferretti wrote: Hi Bertrand, We need to get the Image J course on the map before somebody else thinks of it! Best, Patrizia CONFOCAL MICROSCOPY 4-Day Intensive Hands-On Course Website: www.york.ac.uk/depts/biol/tf/imaging_course.htm Basics, Live-cell imaging, FRET, FRAP and Spectral Unmixing 20-23 April 2010 Technology Facility, Dept. Biology University of York, UK Instructors will include Peter O'Toole, University of York Jo Marrison, University of York Les Borland, Carl Zeiss Colin Hornby, Perkin Elmer and special guest lecture and practical session with Daniel Zicha (CRUK). Tutorials and practical sessions will cover both basic and advanced confocal microscopy. The course will enable participants to realise the potential behind many developing confocal techniques and realise the simplicity of applying the techniques to a broad range of sample types and fluorescent labels. Both novice and more experienced users welcome. Preliminary Programme DAY AIMS Day 1: Basics, Familiarisation, x,y,z and multicolour imaging. Day 2: Live-cell imaging with spinning disk confocal microscopy, FRAP on laser scanning confocal. Day 3: Advanced Techniques covering spectral unmixing and FRET with fluorescent proteins. Day 4: Further advances. More samples to explore confocal limits, and time for participants own samples. Thursday evening will include a special guest speaker and course meal. The course will utilise many different sample types ranging from cultured monolayers to plant cells. A diverse range of fluorescent labels will also be used, which will include various fluorescent proteins (CFP, GFP and YFP) and classic antibody labels. Over the four days, at least four confocal microscopes (2x Zeiss LSM 710 and 2x LSM 510 META on both an invert and upright microscopes, PerkinElmer UltraVIEW LCI or VoX) will be utilised. Accommodation (en-suite), breakfast and lunches will be provided for the length of the course. Evening meals will be provided on both Tuesday and Thursday evenings. Places are limited to 12 participants to permit full hands-on practice, so please book early to avoid disappointment. For further information, please visit the course website at: www.york.ac.uk/depts/biol/tf/imaging-cytometry/imaging_course.htm or contact Margaret Newton 01904 328821, [hidden email] -- Dr Peter O'Toole Head of Imaging and Cytometry Technology Facility Department of Biology (Area 15) University of York PO Box 373 YORK YO10 5YW Tel : +44 (0)1904 328722 Fax : +44 (0)1904 328804 email : [hidden email] www.york.ac.uk/biology/tf -- (Sent from my cra%#y non-Blackberry electronic device that still has wires) ********************************* John Runions, Ph.D. School of Life Sciences Oxford Brookes University Oxford, UK OX3 0BP email: [hidden email] phone: +44 (0) 1865 483 964 Runions´lab web site Visit The Illuminated Plant Cell dot com Oxford Brookes Master's in Bioimaging with Molecular Technology |
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