Hardware for z-drift on IX81 Olympus

[Kaarjel Narayanasamy]

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Hello everyone,

I am hoping to get some advice and recommendations for microscope hardware. We have an Olympus IX81 microscope without focus stabilization. I was wondering if anyone has any opinions on what would work best for z-drift correction, for example if the IX2-NPS or the C-focus from madcitylabs is worth getting. Any other suggestions are welcome!

Thanks in advance.

Best wishes,
Kaarjel

Kaarjel Narayanasamy, PhD
Dept. of Functional Neuroanatomy
Heidelberg University
Im Neuenheimer Feld 307
69120 Heidelberg, Germany
Email: [hidden email]

[mmodel]

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We used to use the autofocus function in Slidebook. It may take too long an exposure to do it in fluorescence because of photobleaching but it worked in transmission (if you need fluorescence and can automatically switch between transmission and fluorescence)

Mike

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Kaarjel Narayanasamy
Sent: Tuesday, September 22, 2020 9:03 AM
To: [hidden email]
Subject: Hardware for z-drift on IX81 Olympus

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Hello everyone,

I am hoping to get some advice and recommendations for microscope hardware. We have an Olympus IX81 microscope without focus stabilization. I was wondering if anyone has any opinions on what would work best for z-drift correction, for example if the IX2-NPS or the C-focus from madcitylabs is worth getting. Any other suggestions are welcome!

Thanks in advance.

Best wishes,
Kaarjel

Kaarjel Narayanasamy, PhD
Dept. of Functional Neuroanatomy
Heidelberg University
Im Neuenheimer Feld 307
69120 Heidelberg, Germany
Email: [hidden email]

[CHOBLET Samuel]

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It is somehow a commercial post

*********************************

Dear Kaarjel,

For focus stabilization, we have a number of our customers using  our piezostages together with the CRISP, a Focus Stabilisation System proposed by ASI Imaging : http://www.asiimaging.com/products/focus-control-and-stabilization/crisp-autofocus-system/

The advantage of the CRISP is that it does a direct measurement of the distance between the objective and a (reflective) reference surface located on your sample. This distance is the distance to measure in order to keep the same focus over hours.

Regarding the C-focus, it measures only the drift between the bottom of your objective (where it is screwed) and the bottom of your microstage. The C-focus doesn't measure the distance between the objective and the sample, like the CRISP from ASI does.  If your sample moves up or down, the C-focus will not see that movement. If your microscope objective elongates (because of thermal expansion for example), the C-focus will not measure that drift either.

Please note that the CRISP from ASI Imaging is compatible with our piezostage (together with a MS2000 controller). The CRISP can be used as a stand alone system.

Please let me know if I can help any further.

Best regards,

Samuel CHOBLET

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PIEZOCONCEPT
www.piezoconcept.com
15 Rue du Bocage
69008 LYON (France)
Phone Europe : [phone]
Phone USA : [phone]
Email : [hidden email]
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-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Kaarjel Narayanasamy
Sent: mardi 22 septembre 2020 15:03
To: [hidden email]
Subject: Hardware for z-drift on IX81 Olympus

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Hello everyone,

I am hoping to get some advice and recommendations for microscope hardware. We have an Olympus IX81 microscope without focus stabilization. I was wondering if anyone has any opinions on what would work best for z-drift correction, for example if the IX2-NPS or the C-focus from madcitylabs is worth getting. Any other suggestions are welcome!

Thanks in advance.

Best wishes,
Kaarjel

Kaarjel Narayanasamy, PhD
Dept. of Functional Neuroanatomy
Heidelberg University
Im Neuenheimer Feld 307
69120 Heidelberg, Germany
Email: [hidden email]


--
L'absence de virus dans ce courrier électronique a été vérifiée par le logiciel antivirus Avast.
https://www.avast.com/antivirus

[Zdenek Svindrych-2]

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Hi Kaarjel,

the Olympus IX2-NPS is just a nosepiece stage, it does not compensate
z-drift, it only tries to minimize it by coupling the sample directly to
the objective turret.
See here: https://drive.google.com/file/d/1KvpmeRsvUOB6n1JaZQLWwVrp93gIdXny

It helps with the z-drift, but the severe downside is that you can't use
the IX81 motor for focusing (this is done manually by turning the NPS). You
can use your manual XY stage to move with the sample, but before you start
imaging you need to make sure the sample plate is not mechanically coupled
to the stage (e.g. by raising the nosepiece).

And it blocks many positions in the objective turret, I'm not sure you can
use other objectives at all (the NPS is bulky,and the whole stage needs to
be raised up by a centimeter or so).

Best, zdenek


On Tue, Sep 22, 2020 at 9:14 AM Kaarjel Narayanasamy <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello everyone,
>
> I am hoping to get some advice and recommendations for microscope
> hardware. We have an Olympus IX81 microscope without focus stabilization. I
> was wondering if anyone has any opinions on what would work best for
> z-drift correction, for example if the IX2-NPS or the C-focus from
> madcitylabs is worth getting. Any other suggestions are welcome!
>
> Thanks in advance.
>
> Best wishes,
> Kaarjel
>
> Kaarjel Narayanasamy, PhD
> Dept. of Functional Neuroanatomy
> Heidelberg University
> Im Neuenheimer Feld 307
> 69120 Heidelberg, Germany
> Email: [hidden email]
>

--
--
Zdenek Svindrych, Ph.D.
Research Scientist - Microscopy Imaging Specialist
Department of Biochemistry and Cell Biology
Geisel School of Medicine at Dartmouth

[Alistair Curd]

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Hi Kaarjel,

The C-focus also involves raising the objective and sample up, compared to the usual position (and other objectives), because the objective needs to go in an extra mounting component that includes part of the Z-sensing apparatus (at least in our model from several years ago). That objective mount screws into the IX81 turret and adds a similar ~1 cm to the tube length, which may then be sub-optimal for working with, or may be fine, depending on your illumination scheme.

Best wishes,

Alistair Curd
School of Molecular and Cellular Biology
Faculty of Biological Sciences
University of Leeds
Leeds LS2 9JT
UK

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Zdenek Svindrych
Sent: 22 September 2020 16:17
To: [hidden email]
Subject: Re: Hardware for z-drift on IX81 Olympus

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Hi Kaarjel,

the Olympus IX2-NPS is just a nosepiece stage, it does not compensate z-drift, it only tries to minimize it by coupling the sample directly to the objective turret.
See here: https://drive.google.com/file/d/1KvpmeRsvUOB6n1JaZQLWwVrp93gIdXny

It helps with the z-drift, but the severe downside is that you can't use the IX81 motor for focusing (this is done manually by turning the NPS). You can use your manual XY stage to move with the sample, but before you start imaging you need to make sure the sample plate is not mechanically coupled to the stage (e.g. by raising the nosepiece).

And it blocks many positions in the objective turret, I'm not sure you can use other objectives at all (the NPS is bulky,and the whole stage needs to be raised up by a centimeter or so).

Best, zdenek


On Tue, Sep 22, 2020 at 9:14 AM Kaarjel Narayanasamy < [hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello everyone,
>
> I am hoping to get some advice and recommendations for microscope
> hardware. We have an Olympus IX81 microscope without focus
> stabilization. I was wondering if anyone has any opinions on what
> would work best for z-drift correction, for example if the IX2-NPS or
> the C-focus from madcitylabs is worth getting. Any other suggestions are welcome!
>
> Thanks in advance.
>
> Best wishes,
> Kaarjel
>
> Kaarjel Narayanasamy, PhD
> Dept. of Functional Neuroanatomy
> Heidelberg University
> Im Neuenheimer Feld 307
> 69120 Heidelberg, Germany
> Email: [hidden email]
>

--
--
Zdenek Svindrych, Ph.D.
Research Scientist - Microscopy Imaging Specialist Department of Biochemistry and Cell Biology Geisel School of Medicine at Dartmouth

[Dan Focht]

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Kaarjel


How old is the scope?
My concern is the age of the lubricants in the focus system.
As they age they turn from a grease to a wax.
Once they become wax they inhibit motion and can cause focus to craw or drift.


Dan Focht
[hidden email]



On Sep 22, 2020, at 9:02 AM, Kaarjel Narayanasamy <[hidden email]> wrote:

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Post images on http://www.imgur.com and include the link in your posting.
*****

Hello everyone,

I am hoping to get some advice and recommendations for microscope hardware. We have an Olympus IX81 microscope without focus stabilization. I was wondering if anyone has any opinions on what would work best for z-drift correction, for example if the IX2-NPS or the C-focus from madcitylabs is worth getting. Any other suggestions are welcome!

Thanks in advance.

Best wishes,
Kaarjel

Kaarjel Narayanasamy, PhD
Dept. of Functional Neuroanatomy
Heidelberg University
Im Neuenheimer Feld 307
69120 Heidelberg, Germany
Email: [hidden email]