Help with stage issues?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Everyone, I am having some strange problems with the stage on one of my widefield scopes and I am wondering if anyone has any suggestions? Sometimes the stage seems to freeze and only wants to travel one direction in Y, but the opposite direction in Y is fine. Driving all the way in the possible direction in Y sometimes fixes this. Travel in the X direction is unaffected. Other times the stage moves by itself in Y. Both problems are sporadic, but now seem to be happening more frequently. I am not sure if the problem is most likely from the controller, joystick or stage. I have also occasionally started to get time out warnings about the shutter when I try to snap an image, which makes me think it is the controller. I have a Ludl Mac5000 controller running a Ludl 99S106 stage with a MAC2 XY joystick and am running it with ZEN 2.3. Offline/online responses welcome. Thanks, Ben -- Benjamin Abrams, Ph.D. (He/Him) Director, UCSC Life Sciences Microscopy Center https://ibsc.ucsc.edu/facilities/microscopy RRID: SCR_021135 |
 
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Stout, Andrea |
Re: [External] Help with stage issues?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Ben, We had a very similar problem with our Ludl stage and Mac5000 controller last year. After many months of trying to track down the problem (and eliminating the joystick as the source) we finally sent the controller and stage back to Ludl. It turned out to be a board inside the controller that had gone bad. They were able to repair it pretty quickly. Good luck! Andrea Andrea L. Stout, Ph. D. Director, CDB Microscopy Core Facility Perelman School of Medicine at the University of Pennsylvania 1107 BRB 2/3 421 Curie Boulevard Philadelphia, PA 19104 pronouns: she/her phone: 215-573-3942 email: [hidden email]<mailto:[hidden email]> web: http://www.med.upenn.edu/cdbmicroscopycore/ Twitter: @CDBMicroCore On May 10, 2021, at 6:37 PM, Ben Abrams <[hidden email]<mailto:[hidden email]>> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Everyone, I am having some strange problems with the stage on one of my widefield scopes and I am wondering if anyone has any suggestions? Sometimes the stage seems to freeze and only wants to travel one direction in Y, but the opposite direction in Y is fine. Driving all the way in the possible direction in Y sometimes fixes this. Travel in the X direction is unaffected. Other times the stage moves by itself in Y. Both problems are sporadic, but now seem to be happening more frequently. I am not sure if the problem is most likely from the controller, joystick or stage. I have also occasionally started to get time out warnings about the shutter when I try to snap an image, which makes me think it is the controller. I have a Ludl Mac5000 controller running a Ludl 99S106 stage with a MAC2 XY joystick and am running it with ZEN 2.3. Offline/online responses welcome. Thanks, Ben -- Benjamin Abrams, Ph.D. (He/Him) Director, UCSC Life Sciences Microscopy Center https://ibsc.ucsc.edu/facilities/microscopy RRID: SCR_021135 Andrea L. Stout, Ph. D. Director, CDB Microscopy Core Facility Perelman School of Medicine at the University of Pennsylvania 1107 BRB 2/3 421 Curie Boulevard Philadelphia, PA 19104 pronouns: she/her phone: 215-573-3942 email: [hidden email]<mailto:[hidden email]> web: http://www.med.upenn.edu/cdbmicroscopycore/ Twitter: @CDBMicroCore |
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In reply to this post by Ben Abrams-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Ben, We recently had some issues with our VS120 Slide Scanner stage which ended up being a problem with the comms card in the PC (we could push/bend the card and it would work). We also swapped the cables around going into the stage to see if the problem persisted on the same direction or swapped to the other axis to eliminate a stage motor issue, other than that if you can find someone with the same controller you could swap in for a test that should help to narrow down the location of the problem. Cheers Mark ---------------------------------- Mark Scott Microscopy Core Facility Manager Translational Research Institute Brisbane, Australia -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Ben Abrams Sent: Tuesday, 11 May 2021 8:38 AM To: [hidden email] Subject: Help with stage issues? <table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" align="left" width="100%" style="width:100.0%"> <tbody> <tr> <td style="background:#910A19;padding:0pt 2pt 0pt 2pt"></td> <td width="100%" style="width:100.0%;background:#FDF2F4;padding:0pt 2pt 0pt 2pt" color="#212121"> <div> <p> <span style="font-size:9.0pt;font-family: sans-serif;color:#212121"> This email has been identified as possessing SPAM like characteristics, please proceed with care. Do not click links or open attachments unless you recognise the source of this email and know the content is safe. </span> </p> <p> <span style="font-size:9.0pt;font-family: sans-serif;color:#212121"> If you have any concerns please contact TRI ICT. </span> </p> </div> </td> </tr> </tbody> </table> ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Everyone, I am having some strange problems with the stage on one of my widefield scopes and I am wondering if anyone has any suggestions? Sometimes the stage seems to freeze and only wants to travel one direction in Y, but the opposite direction in Y is fine. Driving all the way in the possible direction in Y sometimes fixes this. Travel in the X direction is unaffected. Other times the stage moves by itself in Y. Both problems are sporadic, but now seem to be happening more frequently. I am not sure if the problem is most likely from the controller, joystick or stage. I have also occasionally started to get time out warnings about the shutter when I try to snap an image, which makes me think it is the controller. I have a Ludl Mac5000 controller running a Ludl 99S106 stage with a MAC2 XY joystick and am running it with ZEN 2.3. Offline/online responses welcome. Thanks, Ben -- Benjamin Abrams, Ph.D. (He/Him) Director, UCSC Life Sciences Microscopy Center https://ibsc.ucsc.edu/facilities/microscopy RRID: SCR_021135 |
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Stanislav Vitha-2 |
Re: Help with stage issues?
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In reply to this post by Ben Abrams-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** If it was just the stage moving by itself, I would be suspecting the joystick. The springs holding the joystick may get tired and do not hold the stick in a neutral position (that is what we had happen on our old Olympus confocal system, which uses essentially the same joystick to drive a Prior stage). But given the other symptoms, I would say it could be the controller. If you swap the X and Y cables, is it still the same axis having problems? Stan Vitha Texas A&M University Microscopy and Imaging Center http://microscopy.tamu.edu On Mon, 10 May 2021 17:37:43 -0500, Ben Abrams <[hidden email]> wrote: >Hi Everyone, > >I am having some strange problems with the stage on one of my widefield scopes and I am wondering if anyone has any suggestions? Sometimes the stage seems to freeze and only wants to travel one direction in Y, but the opposite direction in Y is fine. Driving all the way in the possible direction in Y sometimes fixes this. Travel in the X direction is unaffected. >Other times the stage moves by itself in Y. Both problems are sporadic, but now seem to be happening more frequently. I am not sure if the problem is most likely from the controller, joystick or stage. I have also occasionally started to get time out warnings about the shutter when I try to snap an image, which makes me think it is the controller. > >I have a Ludl Mac5000 controller running a Ludl 99S106 stage with a MAC2 XY joystick and am running it with ZEN 2.3. > >Offline/online responses welcome. > > >Thanks, >Ben > >-- >Benjamin Abrams, Ph.D. >(He/Him) >Director, UCSC Life Sciences Microscopy Center >https://ibsc.ucsc.edu/facilities/microscopy >RRID: SCR_021135 |
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In reply to this post by Ben Abrams-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Ben, I experienced almost exactly the same problems with our 13 year old still heavily used Prior stage H101A and its controller PS2. We consulted with Prior, brought a new controller SP3, and sent the stage, controller and joystick back for a factory maintain/repair service. We have been using the repaired stage for 15 months without any problem since. Conclusion: The stage needs cleaned and lubricated. The old controller got circuit component failed and has to be replaced with an upgrade version. The joystick was wearied out and replaced with the same version. Best regards, Jingsong Wang (He, him, his), Ph.D Imaging Facility Manager BC Children's Hospital Research Institute -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Ben Abrams Sent: May 10, 2021 3:38 PM To: [hidden email] Subject: Help with stage issues? [CAUTION: Non-UBC Email] ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Everyone, I am having some strange problems with the stage on one of my widefield scopes and I am wondering if anyone has any suggestions? Sometimes the stage seems to freeze and only wants to travel one direction in Y, but the opposite direction in Y is fine. Driving all the way in the possible direction in Y sometimes fixes this. Travel in the X direction is unaffected. Other times the stage moves by itself in Y. Both problems are sporadic, but now seem to be happening more frequently. I am not sure if the problem is most likely from the controller, joystick or stage. I have also occasionally started to get time out warnings about the shutter when I try to snap an image, which makes me think it is the controller. I have a Ludl Mac5000 controller running a Ludl 99S106 stage with a MAC2 XY joystick and am running it with ZEN 2.3. Offline/online responses welcome. Thanks, Ben -- Benjamin Abrams, Ph.D. (He/Him) Director, UCSC Life Sciences Microscopy Center https://ibsc.ucsc.edu/facilities/microscopy RRID: SCR_021135 |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Ben, I hesitate to send this reply but it might be helpful if not to you, to someone else on the listserv. We had some issues recently with a Marzhauser XY stage connected to our Zeiss LSM 710. The main problem we had is that the stage kept moving in the X direction after release of the joystick and then we started also getting errors in ZEN with the stage not being detected. We cleaned the joystick by dismantling it and using contact cleaner and it didn’t help so Zeiss managed to find us a replacement controller, which solved the problem with the software but we were still getting additional drift when using the joystick. This issue was solved by cleaning the connections at the controller end (joystick and controller) with electronics contact cleaner, each time it happened. For a week or so, I was doing this every day (once/twice a day) but now it’s all good and the issue is rare now and easily fixed. Kind regards, Jacqui From: Confocal Microscopy List <[hidden email]> On Behalf Of Wang, Jingsong Sent: Wednesday, May 12, 2021 6:00 AM To: [hidden email] Subject: Re: Help with stage issues? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on http://www.imgur.com<http://www.imgur.com> and include the link in your posting. ***** Hi Ben, I experienced almost exactly the same problems with our 13 year old still heavily used Prior stage H101A and its controller PS2. We consulted with Prior, brought a new controller SP3, and sent the stage, controller and joystick back for a factory maintain/repair service. We have been using the repaired stage for 15 months without any problem since. Conclusion: The stage needs cleaned and lubricated. The old controller got circuit component failed and has to be replaced with an upgrade version. The joystick was wearied out and replaced with the same version. Best regards, Jingsong Wang (He, him, his), Ph.D Imaging Facility Manager BC Children's Hospital Research Institute -----Original Message----- From: Confocal Microscopy List <[hidden email]<mailto:[hidden email]>> On Behalf Of Ben Abrams Sent: May 10, 2021 3:38 PM To: [hidden email]<mailto:[hidden email]> Subject: Help with stage issues? [CAUTION: Non-UBC Email] ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on http://www.imgur.com<http://www.imgur.com> and include the link in your posting. ***** Hi Everyone, I am having some strange problems with the stage on one of my widefield scopes and I am wondering if anyone has any suggestions? Sometimes the stage seems to freeze and only wants to travel one direction in Y, but the opposite direction in Y is fine. Driving all the way in the possible direction in Y sometimes fixes this. Travel in the X direction is unaffected. Other times the stage moves by itself in Y. Both problems are sporadic, but now seem to be happening more frequently. I am not sure if the problem is most likely from the controller, joystick or stage. I have also occasionally started to get time out warnings about the shutter when I try to snap an image, which makes me think it is the controller. I have a Ludl Mac5000 controller running a Ludl 99S106 stage with a MAC2 XY joystick and am running it with ZEN 2.3. Offline/online responses welcome. Thanks, Ben -- Benjamin Abrams, Ph.D. (He/Him) Director, UCSC Life Sciences Microscopy Center https://ibsc.ucsc.edu/facilities/microscopy<https://ibsc.ucsc.edu/facilities/microscopy> RRID: SCR_021135 |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Several years ago I had two Prior joysticks exhibit similar drift problems, where the stick "at rest" would still send a command signal to begin moving in one direction. If the glitch is intermittent this can lead to your stage very slowly walking away over time. Prior acknowledged the problem and sent replacement units with an updated electromechanical design. Since I have been using the replacement sticks I have had no further drift issues. Craig On Tue, May 11, 2021 at 2:49 PM Jacqueline Ross <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Ben, > > I hesitate to send this reply but it might be helpful if not to you, to > someone else on the listserv. > > We had some issues recently with a Marzhauser XY stage connected to our > Zeiss LSM 710. The main problem we had is that the stage kept moving in the > X direction after release of the joystick and then we started also getting > errors in ZEN with the stage not being detected. We cleaned the joystick by > dismantling it and using contact cleaner and it didn’t help so Zeiss > managed to find us a replacement controller, which solved the problem with > the software but we were still getting additional drift when using the > joystick. This issue was solved by cleaning the connections at the > controller end (joystick and controller) with electronics contact cleaner, > each time it happened. For a week or so, I was doing this every day > (once/twice a day) but now it’s all good and the issue is rare now and > easily fixed. > > Kind regards, > > Jacqui > > From: Confocal Microscopy List <[hidden email]> On > Behalf Of Wang, Jingsong > Sent: Wednesday, May 12, 2021 6:00 AM > To: [hidden email] > Subject: Re: Help with stage issues? > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy< > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > Post images on http://www.imgur.com<http://www.imgur.com> and include the > link in your posting. > ***** > > Hi Ben, > > I experienced almost exactly the same problems with our 13 year old still > heavily used Prior stage H101A and its controller PS2. We consulted with > Prior, brought a new controller SP3, and sent the stage, controller and > joystick back for a factory maintain/repair service. We have been using the > repaired stage for 15 months without any problem since. Conclusion: The > stage needs cleaned and lubricated. The old controller got circuit > component failed and has to be replaced with an upgrade version. The > joystick was wearied out and replaced with the same version. > > > Best regards, > > Jingsong Wang (He, him, his), Ph.D > Imaging Facility Manager > BC Children's Hospital Research Institute > > > -----Original Message----- > From: Confocal Microscopy List <[hidden email]<mailto: > [hidden email]>> On Behalf Of Ben Abrams > Sent: May 10, 2021 3:38 PM > To: [hidden email]<mailto: > [hidden email]> > Subject: Help with stage issues? > > [CAUTION: Non-UBC Email] > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy< > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > Post images on http://www.imgur.com<http://www.imgur.com> and include the > link in your posting. > ***** > > Hi Everyone, > > I am having some strange problems with the stage on one of my widefield > scopes and I am wondering if anyone has any suggestions? Sometimes the > stage seems to freeze and only wants to travel one direction in Y, but the > opposite direction in Y is fine. Driving all the way in the possible > direction in Y sometimes fixes this. Travel in the X direction is > unaffected. > Other times the stage moves by itself in Y. Both problems are sporadic, > but now seem to be happening more frequently. I am not sure if the problem > is most likely from the controller, joystick or stage. I have also > occasionally started to get time out warnings about the shutter when I try > to snap an image, which makes me think it is the controller. > > I have a Ludl Mac5000 controller running a Ludl 99S106 stage with a MAC2 > XY joystick and am running it with ZEN 2.3. > > Offline/online responses welcome. > > > Thanks, > Ben > > -- > Benjamin Abrams, Ph.D. > (He/Him) > Director, UCSC Life Sciences Microscopy Center > https://ibsc.ucsc.edu/facilities/microscopy< > https://ibsc.ucsc.edu/facilities/microscopy> > RRID: SCR_021135 > |
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In reply to this post by Ben Abrams-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Everyone, Thank you for all the helpful suggestions regarding the diagnosis and repair of my stage issues. Much appreciated! Ben -- Benjamin Abrams, Ph.D. (He/Him) Director, UCSC Life Sciences Microscopy Center https://ibsc.ucsc.edu/facilities/microscopy RRID: SCR_021135 |
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