High Content Equipment

[Pedro Camello]

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Hi all,

I would appreciate experiences/opinion for the purchase of a HC imaging system. The goal is work with live cultures, in a variety of formats, and ability for liquid addition would be desirable. Confocal would be a secondary ability.

Many thanks for your help

[Sylvie Le Guyader]

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Hi Pedro

My experience is that you get everything you need and more for less money if you buy a scientific grade microscope with on-the-fly analysis capacity compared to the box-type systems. If you need confocality and widefield, you can buy a spinning disk with bypass.

We use Nikon microscopes with full incubators, the NIS-Elements software with the JOBS module.

Med vänlig hälsning / Best regards

Sylvie

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Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Hälsovägen 7C,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
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-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Pedro Camello
Sent: 19 May 2019 11:11
To: [hidden email]
Subject: High Content Equipment

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Hi all,

I would appreciate experiences/opinion for the purchase of a HC imaging system. The goal is work with live cultures, in a variety of formats, and ability for liquid addition would be desirable. Confocal would be a secondary ability.

Many thanks for your help


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[Tim Feinstein]

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I think you need to specify the scale of your project.  The easiest answer is to go with a stage incubator that accommodates multiwell plates.  A 96-well dish, automated focus control and some experience with R, CellProfiler and ImageJ scripting will get you quite a lot of throughput.  All of the major scope companies can do this or you can get custom solutions from, e.g., Andor, or even Thorlabs if you are ready to help build & maintain it.  On the other hand I don't know of many stage-top incubators that will let you do fluid handling in a 24- or 96-well dish like a plate reader would; Thorlabs _might_ have some ideas about that.  

In general if you need multiwell fluid handling, +/- automated plate exchange, AND a warranty, you may need a "big box" high-content imager.  A good list of available options, both widefield and spinning disc, starts on page 558 of the Lilly Assay Guidance Manual:  

https://www.ncbi.nlm.nih.gov/books/NBK53196/

Best,


T

Timothy Feinstein, Ph.D.
Research Scientist
Department of Developmental Biology
University of Pittsburgh

 


On 5/19/19, 5:24 AM, "Confocal Microscopy List on behalf of Pedro Camello" <[hidden email] on behalf of [hidden email]> wrote:

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    Hi all,
   
    I would appreciate experiences/opinion for the purchase of a HC imaging system. The goal is work with live cultures, in a variety of formats, and ability for liquid addition would be desirable. Confocal would be a secondary ability.
   
    Many thanks for your help