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Reducing the image size or using bidirectional scanning will reduce the time as will reducing the averaging as long as you don't mind trading off image quality for time.
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of George McNamara
Sent: Tuesday, 18 October 2011 3:59 p.m.
To:
[hidden email]
Subject: Re: How to increase scanning speed?
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If LAS AF has "activate resonant scanner" checkbox on startup, do it.
8000 lines/sec. If you do not have it, ask your salesperson for the
upgrade price - ask for pricing on a couple of HyD's as well (see
Wolfgang's recent post).
While waiting for the upgrade(s), set the scan speed to 1400 Hz (max
speed on the standard scanner).
On 10/17/2011 10:09 PM, jax9466 wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Dear All,
> I heard about this list from a lecture on confocal imaging. We have a leica
> Sp5 at the lab, and we've been doing some work on calcium imaging and
> time-lapse. But for our new project, we want to pick up the scanning speed
> of our confocal microscope, 15s is a bit long for us. Is there anyway to
> achieve this goal? Can we do this by simply changing something in settings
> or do we have to contact the maker and purchase an add-on?
>
> Thanks,
> Jun Li
>
> --
> View this message in context:
http://confocal-microscopy-list.588098.n2.nabble.com/How-to-increase-scanning-speed-tp6902989p6902989.html> Sent from the Confocal Microscopy List mailing list archive at Nabble.com.
>
>
--
George McNamara, PhD
Analytical Imaging Core Facility
University of Miami
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