Image from CSU22 not square?

[Moulding, Dale]

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Dear Listers,

I've had the good fortune to have been given an old(ish) Perkin Elmer Ultraview Spinning disk system. Having re-assembled it I'm pleased to find it is in full working order, but I have one possible problem. The images detected on the Hamamatsu EMCCD C9100 are missing the top and bottom. I have no documentation with the system so don't know if this is normal. Image here: http://imgur.com/scW5p5H
I've had a scan through the listserv archives and read that the output from the Yokogawa disk isn't square. Have I got a problem with the system, or should I just crop the camera acquisition area?
cheers
Dale

[WILDE Geraint]

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Dear Dale,

The CSU-22 was designed for use with a rectangular shape CCD (such as the Sony Interline).  Your EMCCD sensor is square.  The shorter vertical aspect of the CSU-22 output is therefore not filling your EMCCD sensor.  You have two options to my knowledge

1. crop, as you suggested
2. see if you can add somewhere around a 1.2x magnification adapter between the CSU-22 and the camera.

Option 2 will be challenging commercially as the CSU-22 is now obsolete, and PE no longer make the UltraView.

Kind regards

Geraint

Geraint Wilde Ph.D.
Product Manager - Microscopy Systems Division
Andor Technology
Tel: [phone]
www.andor.com/microscopy-systems



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Dale Moulding
Sent: 01 November 2016 13:58
To: [hidden email]
Subject: [CONFOCALMICROSCOPY] Image from CSU22 not square?

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Dear Listers,

I've had the good fortune to have been given an old(ish) Perkin Elmer Ultraview Spinning disk system. Having re-assembled it I'm pleased to find it is in full working order, but I have one possible problem. The images detected on the Hamamatsu EMCCD C9100 are missing the top and bottom. I have no documentation with the system so don't know if this is normal. Image here: http://imgur.com/scW5p5H I've had a scan through the listserv archives and read that the output from the Yokogawa disk isn't square. Have I got a problem with the system, or should I just crop the camera acquisition area?
cheers
Dale


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[jerie]

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Dale,

there is a rectangular field stop inside the CSU22 in order to stop scattered light from reaching the cameraas far as I recall, which can be removed or swapped for a square field stop. 

Alternatively, you can magnify the image using a suitable camera c-mount. The overall magnification affects your pinhole diameter, but if you combine a 60x objective with a 1,6x camera mount, that should help.

My 2 cents,

Good luck,

Jens

On Tue, Nov 1, 2016 at 11:58 AM, Dale Moulding <[hidden email]> wrote:

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Dear Listers,

I've had the good fortune to have been given an old(ish) Perkin Elmer Ultraview Spinning disk system. Having re-assembled it I'm pleased to find it is in full working order, but I have one possible problem. The images detected on the Hamamatsu EMCCD C9100 are missing the top and bottom. I have no documentation with the system so don't know if this is normal. Image here: http://imgur.com/scW5p5H
I've had a scan through the listserv archives and read that the output from the Yokogawa disk isn't square. Have I got a problem with the system, or should I just crop the camera acquisition area?
cheers
Dale

[Moulding, Dale]

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Thanks for the replies, both here and off list. I'll stick to cropping the sensor area for now, and give some thought to putting in a magnifying camera mount. Mostly just relieved that the rectangular output is as it should be!
cheers
Dale