ImageJ scale bar

[Boswell, Carl A - (cboswell)]

Hi all,
I've looked but cannot find a routine to insert a calibrated scale bar into
an image using ImageJ.  Is there a method?
Thanks,
Carl

Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-954-7053
FAX [phone]

[Julio Vazquez]

Hi Carl, 

First you calibrate your pixel dimensions under Image> Properties (unit = microns, and enter x,y values). Then you go to Analyze > Tools... > Scale bar.

Julio.

--

Julio Vazquez

Fred Hutchinson Cancer Research Center

Seattle, WA 98109-1024

http://www.fhcrc.org/

==

On Jul 8, 2009, at 10:52 AM, Carl Boswell wrote:

Hi all,

I've looked but cannot find a routine to insert a calibrated scale bar into an image using ImageJ.  Is there a method?

Thanks,

Carl

Carl A. Boswell, Ph.D.

Molecular and Cellular Biology

University of Arizona

520-954-7053

FAX [phone] 

[Dale Callaham]

Hi Carl,

If you do it much there is a plugin  "Microscope Scale"
http://rsb.info.nih.gov/ij/plugins/microscope-scale.html
that can be edited with the values you get for your system so that you
have them conveniently available. If you work with several systems with
different calibrations that is easy also - check the info on my webpage
on how to set this up - includes a detailed "HOWTO", etc:
Image Calibrations with ImageJ -
http://www.bio.umass.edu/microscopy/resource.htm
http://www.bio.umass.edu/microscopy/Downloads/Calibrations_HOWTO.html

This is entirely a functional adaptation of the Microscope scale plugin
to illustrate how to have multiple calibrations available from different
systems.

Hope this helps.

Dale


Carl Boswell wrote:

> Hi all,
> I've looked but cannot find a routine to insert a calibrated scale bar
> into an image using ImageJ.  Is there a method?
> Thanks,
> Carl
>
> Carl A. Boswell, Ph.D.
> Molecular and Cellular Biology
> University of Arizona
> 520-954-7053
> FAX [phone]

[Daniel James White]

Hi Carl,

for imageJ scale calibration , in a simple tutorial,
look at :

http://pacific.mpi-cbg.de/wiki/index.php/SpatialCalibration

by the way....

FIJI is jiust imageJ - batteries included
http://pacific.mpi-cbg.de/


cheers

Dan



On Jul 9, 2009, at 7:08 AM, CONFOCALMICROSCOPY automatic digest system  
wrote:

> Date:    Wed, 8 Jul 2009 10:52:30 -0700
> From:    Carl Boswell <[hidden email]>
> Subject: ImageJ scale bar
>
> Hi all,
> I've looked but cannot find a routine to insert a calibrated scale  
> bar into
> an image using ImageJ.  Is there a method?
> Thanks,
> Carl
>
> Carl A. Boswell, Ph.D.
> Molecular and Cellular Biology
> University of Arizona
> 520-954-7053
> FAX [phone]

Dr. Daniel James White BSc. (Hons.) PhD
Senior Microscopist / Image Visualisation, Processing and Analysis
Light Microscopy and Image Processing Facilities
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108
01307 DRESDEN
Germany

[phone] (German Mobile)
+49  (0)351 210 2627 (Work phone at MPI-CBG)
+49  (0)351 210 1078 (Fax MPI-CBG LMF)

http://www.bioimagexd.net  BioImageXD
http://pacific.mpi-cbg.de Fiji Fiji is just ImageJ - Batteries Included
http://www.chalkie.org.uk
[hidden email]
( [hidden email] )