Imaging back focal plane of objective

[Shalin Mehta]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear all,

 Is there a convenient threaded mount for eye-piece barrel (that replaces the eye-piece) on which we can mount our own camera (consumer grade)? We wish to take images of back focal plane of the objectives for teaching sake and in future analysis sake.
I checked with Olympus engineer and they do not have it. They do have bertrand lens but it has to be mounted and used with one microscope only. It will be good if we could carry around that eye-piece mount and camera. As a last resort, we could get it machined, but just checking if it is available commercially..

Thanks
Best regards,
Shalin


--
~~~~~~~~~~~~~~~~~~~~~~~~~
Shalin Mehta
Graduate Student in Bioengineering, NUS
mobile: [phone]
blog: shalin.wordpress.com
~~~~~~~~~~~~~~~~~~~~~~~~~~

Search the CONFOCAL archive at
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[Julio Vazquez]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal -

Hi Shalin, 

There are typically two ways to observe the BFP: the Bertrand lens, which is inserted in the optical tube, just before the eyepiece, or the phase centering telescope, which replaces the conventional eyepiece:

http://www.microscopyu.com/articles/phasecontrast/phaseconfiguration.html

There are two ways you can capture images with a consumer camera: by using an eyepiece adapter, or by using a trinocular head with a third port with a camera mount. In either case, you can take pictures of the BFP by combining the Bertrand lens and one of the camera adapters.

Microscope manufacturers should have adapters for their microscopes, but there are also people who sell universal adapters, such as here:

http://www.scopetronics.com/mvp.htm

In particular, this site reports one such adapter combined with a telescope. This is probably what you need.

At the site below, they report taking conoscopic images of crystals using a phase telescope, probably using a similar adapter

http://users.skynet.be/jm-derochette/Conoscopy/Uniaxial_minerals.htm

I don't know who else might sell such devices, and/or how easy it would be to manufacture your own...

--

Julio Vazquez

Fred Hutchinson Cancer Research Center

Seattle, WA 98109-1024

http://www.fhcrc.org

 
==

On Dec 9, 2007, at 1:48 AM, Shalin Mehta wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear all,

 Is there a convenient threaded mount for eye-piece barrel (that replaces the eye-piece) on which we can mount our own camera (consumer grade)? We wish to take images of back focal plane of the objectives for teaching sake and in future analysis sake.
I checked with Olympus engineer and they do not have it. They do have bertrand lens but it has to be mounted and used with one microscope only. It will be good if we could carry around that eye-piece mount and camera. As a last resort, we could get it machined, but just checking if it is available commercially..

Thanks
Best regards,
Shalin


--
~~~~~~~~~~~~~~~~~~~~~~~~~
Shalin Mehta
Graduate Student in Bioengineering, NUS
mobile: [phone]
blog: shalin.wordpress.com
~~~~~~~~~~~~~~~~~~~~~~~~~~

[Shalin Mehta]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Thanks Julio for all the info.,

Scopetronics adapters should solve the issue at 20% of the price required to buy bertrand lens attachment. My initial plan was to image the BFP without phase telescope or bertrand lens by focusing camera down the barrel on the BFP. We have a phase telescope lens, so now I will try to see if its top element (that works as eye lens) can be substituted by one of the scopetronics adapters.

Regards,
Shalin

On Dec 10, 2007 3:46 AM, Julio Vazquez <[hidden email] > wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

-

Hi Shalin, 

There are typically two ways to observe the BFP: the Bertrand lens, which is inserted in the optical tube, just before the eyepiece, or the phase centering telescope, which replaces the conventional eyepiece:

http://www.microscopyu.com/articles/phasecontrast/phaseconfiguration.html

There are two ways you can capture images with a consumer camera: by using an eyepiece adapter, or by using a trinocular head with a third port with a camera mount. In either case, you can take pictures of the BFP by combining the Bertrand lens and one of the camera adapters.

Microscope manufacturers should have adapters for their microscopes, but there are also people who sell universal adapters, such as here:

http://www.scopetronics.com/mvp.htm

In particular, this site reports one such adapter combined with a telescope. This is probably what you need.

At the site below, they report taking conoscopic images of crystals using a phase telescope, probably using a similar adapter

http://users.skynet.be/jm-derochette/Conoscopy/Uniaxial_minerals.htm

I don't know who else might sell such devices, and/or how easy it would be to manufacture your own...

--

Julio Vazquez

Fred Hutchinson Cancer Research Center

Seattle, WA 98109-1024

http://www.fhcrc.org

 
==

On Dec 9, 2007, at 1:48 AM, Shalin Mehta wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear all,

 Is there a convenient threaded mount for eye-piece barrel (that replaces the eye-piece) on which we can mount our own camera (consumer grade)? We wish to take images of back focal plane of the objectives for teaching sake and in future analysis sake.
I checked with Olympus engineer and they do not have it. They do have bertrand lens but it has to be mounted and used with one microscope only. It will be good if we could carry around that eye-piece mount and camera. As a last resort, we could get it machined, but just checking if it is available commercially..

Thanks
Best regards,
Shalin


--
~~~~~~~~~~~~~~~~~~~~~~~~~
Shalin Mehta
Graduate Student in Bioengineering, NUS
mobile: [phone]
blog: shalin.wordpress.com
~~~~~~~~~~~~~~~~~~~~~~~~~~

--
~~~~~~~~~~~~~~~~~~~~~~~~~
Shalin Mehta
Graduate Student in Bioengineering, NUS
mobile: [phone]
blog: shalin.wordpress.com
~~~~~~~~~~~~~~~~~~~~~~~~~~