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Immersion Oil

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Knecht, David

Immersion Oil

We now have Nikon, Zeiss and Leica microscopes in our facility. Is the problem of cross-contamination of immersion oil real or hypothetical? Is it possible to use a single type of immersion oil for all, so that we don't have to worry about moving samples between microscopes? Dave

Dr. David Knecht

Department of Molecular and Cell Biology

Co-head Flow Cytometry and Confocal Microscopy Facility

U-3125

91 N. Eagleville Rd.

University of Connecticut

Storrs, CT 06269

860-486-2200

860-486-4331 (fax)

Theresa Swayne

Re: Immersion Oil

My boss uses Nikon oil on Nikon & Zeiss scopes with no problems. I heard bad stories about cross-contamination (oil combining to form sticky mess) but never tested it.

On Mar 17, 2009, at 9:56 AM, David Knecht wrote:

We now have Nikon, Zeiss and Leica microscopes in our facility. Is the problem of cross-contamination of immersion oil real or hypothetical? Is it possible to use a single type of immersion oil for all, so that we don't have to worry about moving samples between microscopes? Dave

------------------------------------

Theresa C. Swayne, Ph.D.

Manager, Confocal and Specialized Microscopy Shared Resource

Herbert Irving Comprehensive Cancer Center, Columbia University

1130 Saint Nicholas Ave, 222A

New York, NY 10032

212-851-4613

[hidden email]

http://hiccc.columbia.edu/?page=research/sharedresources/confocal

Monique Vasseur

Re: Immersion Oil

In reply to this post by Knecht, David

I have been told by a Nikon representative that Zeiss oil could destroy the Nikon objective lens scellant. I use Cargill immersion oils on Nikon and Olympus microscopes for 7 years now without problems.

Monique Vasseur

Microscopie et imagerie

Département de biochimie

Université de Montréal

C.P. 6128, succursale Centre-ville

Montréal QC H3C 3J7 Canada

tél. (514) 343-6111 poste 5148

De : Confocal Microscopy List [mailto:[hidden email]] De la part de David Knecht
Envoyé : 17 mars 2009 09:57
À : [hidden email]
Objet : Immersion Oil

Dr. David Knecht

Department of Molecular and Cell Biology

Co-head Flow Cytometry and Confocal Microscopy Facility

U-3125

91 N. Eagleville Rd.

University of Connecticut

Storrs, CT 06269

860-486-2200

860-486-4331 (fax)

Craig Brideau

Re: Immersion Oil

In reply to this post by Theresa Swayne

We use Cargille oil on all our scopes and it seems to work well. Cargille sells a cheap sampler kit that lets you try a few different types of their oils. For uprights type A is good, while type B is good for inverted. They have a few other types with various properties as well.

http://www.cargille.com/immeroil.shtml

Craig

On Tue, Mar 17, 2009 at 9:25 AM, Theresa Swayne <[hidden email]> wrote:

My boss uses Nikon oil on Nikon & Zeiss scopes with no problems. I heard bad stories about cross-contamination (oil combining to form sticky mess) but never tested it.

On Mar 17, 2009, at 9:56 AM, David Knecht wrote:

We now have Nikon, Zeiss and Leica microscopes in our facility. Is the problem of cross-contamination of immersion oil real or hypothetical? Is it possible to use a single type of immersion oil for all, so that we don't have to worry about moving samples between microscopes? Dave

------------------------------------
Theresa C. Swayne, Ph.D.
Manager, Confocal and Specialized Microscopy Shared Resource

Herbert Irving Comprehensive Cancer Center, Columbia University
1130 Saint Nicholas Ave, 222A

New York, NY 10032

212-851-4613

[hidden email]

http://hiccc.columbia.edu/?page=research/sharedresources/confocal

Peter Carroll

Re: Immersion Oil

In reply to this post by Monique Vasseur

> I have been told by a Nikon representative that Zeiss oil could
destroy the Nikon objective lens

Of course you heard that... Nikon wants you to purchase /their/
consumables, not their competitors! Heh...

Knecht, David

Re: Immersion Oil

Being a natural skeptic, that has been my presumption as well. I haven't talked to anyone who has actually seen mixed oils gum up their scope or dissolve lens cement (if you believe that last one, I have a few million dollars in my uncle's account in Africa I will be happy to send you). Dave

On Mar 17, 2009, at 11:37 AM, Peter Carroll wrote:

> I have been told by a Nikon representative that Zeiss oil could destroy the Nikon objective lens

Of course you heard that... Nikon wants you to purchase /their/ consumables, not their competitors! Heh...

Dr. David Knecht

Department of Molecular and Cell Biology

Co-head Flow Cytometry and Confocal Microscopy Facility

U-3125

91 N. Eagleville Rd.

University of Connecticut

Storrs, CT 06269

860-486-2200

860-486-4331 (fax)

stu_the_flat

Re: Immersion Oil

Isn't there a 0.002 difference in the diffraction between some of the immersion oils?

Off the top of my head

Olympus Oil = 1.515
Ziess Oil = 1.515
Nikon Oil = 1.513

I don’t think it make the world of difference but as I am interested in PSFs I always match the oil the microscope.

Stuart

David Knecht-charter wrote

Being a natural skeptic, that has been my presumption as well. I
haven't talked to anyone who has actually seen mixed oils gum up their
scope or dissolve lens cement (if you believe that last one, I have a
few million dollars in my uncle's account in Africa I will be happy
to send you). Dave

On Mar 17, 2009, at 11:37 AM, Peter Carroll wrote:

>
> > I have been told by a Nikon representative that Zeiss oil could
> destroy the Nikon objective lens
>
> Of course you heard that... Nikon wants you to purchase /their/
> consumables, not their competitors! Heh...

Dr. David Knecht
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)

Craig Brideau

Re: Immersion Oil

When you are down to the third decimal place it's not going to make a huge difference*. You might want to try and see if you observe any difference between oil, but I suspect you won't see much change.

Craig

*unless you are talking about fiber optics or some optoelectronics...

On Tue, Mar 17, 2009 at 9:58 AM, stu_the_flat <[hidden email]> wrote:

Isn't there a 0.002 difference in the diffraction between some of the
immersion oils?

Off the top of my head

Olympus Oil = 1.515
Ziess Oil = 1.515
Nikon Oil = 1.513

I don’t think it make the world of difference but as I am interested in PSFs
I always match the oil the microscope.

Stuart

David Knecht-charter wrote:
>
> Being a natural skeptic, that has been my presumption as well. I
> haven't talked to anyone who has actually seen mixed oils gum up their
> scope or dissolve lens cement (if you believe that last one, I have a
> few million dollars in my uncle's account in Africa I will be happy
> to send you). Dave
>
> On Mar 17, 2009, at 11:37 AM, Peter Carroll wrote:
>
>>
>> > I have been told by a Nikon representative that Zeiss oil could
>> destroy the Nikon objective lens
>>
>> Of course you heard that... Nikon wants you to purchase /their/
>> consumables, not their competitors! Heh...
>
> Dr. David Knecht
> Department of Molecular and Cell Biology
> Co-head Flow Cytometry and Confocal Microscopy Facility
> U-3125
> 91 N. Eagleville Rd.
> University of Connecticut
> Storrs, CT 06269
> 860-486-2200
> 860-486-4331 (fax)
>
>
>
>

--
View this message in context: http://n2.nabble.com/Immersion-Oil-tp2491973p2492239.html
Sent from the Confocal Microscopy List mailing list archive at Nabble.com.

Paul Maddox

Re: Immersion Oil

It’s an easy test to prove that the last (third) decimal place makes a reasonable difference. Using the Cargille test kit (range of ~20 RI around 1.515 step by 0.001), you can acquired PSFs with the different oils and just take a look at the x,z projections. You’ll see a pretty dramatic shift in spherical aberration, even on small steps (ok, 0.001 is pretty small change, but 0.002 change is measurable for sure). It gets even more interesting (dare I say fun) if you mount the beads in some low percentage agar so that you get beads at random distances from the coverglass! I did this one weekend and recommend it if you need a distraction (from grants for instance…).

As for mixing oils, I always thought the big worry was that different brands might not be miscible and therefore you could introduce a new RI boundary, causing aberrations. I have never tested, or in fact seen this demonstrated (on purpose or otherwise). Nonetheless, I will continue to not mix my oils.

Happy imaging,

Paul

Paul S. Maddox, PhD
Assistant Professor
Institute for Research in Immunology and Cancer
Dept of Pathology and Cell Biol, U. de Montreal
P.O. Box 6128, Station Centre-Ville
Montréal QC H3C 3J7
CANADA

Courier:
2900, boulevard Édouard-Montpetit
Pavillon Marcelle-Coutu, Quai 20
Montreal QC H3T 1J4
CANADA

[hidden email]
Ph: 514-343-7894
Fax: 514-343-6843

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Craig Brideau
Sent: Tuesday, March 17, 2009 12:24 PM
To: [hidden email]
Subject: Re: Immersion Oil

On Tue, Mar 17, 2009 at 9:58 AM, stu_the_flat <[hidden email]> wrote:

Isn't there a 0.002 difference in the diffraction between some of the
immersion oils?

Off the top of my head

Olympus Oil = 1.515
Ziess Oil = 1.515
Nikon Oil = 1.513

I don’t think it make the world of difference but as I am interested in PSFs
I always match the oil the microscope.

Stuart

David Knecht-charter wrote:
>
> Being a natural skeptic, that has been my presumption as well. I
> haven't talked to anyone who has actually seen mixed oils gum up their
> scope or dissolve lens cement (if you believe that last one, I have a
> few million dollars in my uncle's account in Africa I will be happy
> to send you). Dave
>
> On Mar 17, 2009, at 11:37 AM, Peter Carroll wrote:
>
>>
>> > I have been told by a Nikon representative that Zeiss oil could
>> destroy the Nikon objective lens
>>
>> Of course you heard that... Nikon wants you to purchase /their/
>> consumables, not their competitors! Heh...
>
> Dr. David Knecht
> Department of Molecular and Cell Biology
> Co-head Flow Cytometry and Confocal Microscopy Facility
> U-3125
> 91 N. Eagleville Rd.
> University of Connecticut
> Storrs, CT 06269
> 860-486-2200
> 860-486-4331 (fax)
>
>
>
>

--
View this message in context: http://n2.nabble.com/Immersion-Oil-tp2491973p2492239.html
Sent from the Confocal Microscopy List mailing list archive at Nabble.com.

Kurt Thorn

Re: Immersion Oil

One thing I have heard is that different oils have different
dispersions, so you may introduce chromatic aberration if you use oils
from different vendors. I would do the experiment with multicolored
beads and look for axial shifts in the different channels before
deciding to switch oils.

Kurt

Maddox Paul wrote:

>
> It’s an easy test to prove that the last (third) decimal place makes a
> reasonable difference. Using the Cargille test kit (range of ~20 RI
> around 1.515 step by 0.001), you can acquired PSFs with the different
> oils and just take a look at the x,z projections. You’ll see a pretty
> dramatic shift in spherical aberration, even on small steps (ok, 0.001
> is pretty small change, but 0.002 change is measurable for sure). It
> gets even more interesting (dare I say fun) if you mount the beads in
> some low percentage agar so that you get beads at random distances
> from the coverglass! I did this one weekend and recommend it if you
> need a distraction (from grants for instance…).
>
> As for mixing oils, I always thought the big worry was that different
> brands might not be miscible and therefore you could introduce a new
> RI boundary, causing aberrations. I have never tested, or in fact seen
> this demonstrated (on purpose or otherwise). Nonetheless, I will
> continue to not mix my oils.
>
> Happy imaging,
>
> Paul
>
> Paul S. Maddox, PhD
> Assistant Professor
> Institute for Research in Immunology and Cancer
> Dept of Pathology and Cell Biol, U. de Montreal
> P.O. Box 6128, Station Centre-Ville
> Montréal QC H3C 3J7
> CANADA
>
> Courier:
> 2900, boulevard Édouard-Montpetit
> Pavillon Marcelle-Coutu, Quai 20
> Montreal QC H3T 1J4
> CANADA
>
> [hidden email] <mailto:[hidden email]>
> Ph: 514-343-7894
> Fax: 514-343-6843
>
> ------------------------------------------------------------------------
>
> *From:* Confocal Microscopy List
> [mailto:[hidden email]] *On Behalf Of *Craig Brideau
> *Sent:* Tuesday, March 17, 2009 12:24 PM
> *To:* [hidden email]
> *Subject:* Re: Immersion Oil
>
> When you are down to the third decimal place it's not going to make a
> huge difference*. You might want to try and see if you observe any
> difference between oil, but I suspect you won't see much change.
>
> Craig
>
> *unless you are talking about fiber optics or some optoelectronics...
>
> On Tue, Mar 17, 2009 at 9:58 AM, stu_the_flat <[hidden email]
> <mailto:[hidden email]>> wrote:
>
> Isn't there a 0.002 difference in the diffraction between some of the
> immersion oils?
>
> Off the top of my head
>
> Olympus Oil = 1.515
> Ziess Oil = 1.515
> Nikon Oil = 1.513
>
> I don’t think it make the world of difference but as I am interested
> in PSFs
> I always match the oil the microscope.
>
> Stuart
>
>
>
>
>
> David Knecht-charter wrote:
> >
> > Being a natural skeptic, that has been my presumption as well. I
> > haven't talked to anyone who has actually seen mixed oils gum up their
> > scope or dissolve lens cement (if you believe that last one, I have a
> > few million dollars in my uncle's account in Africa I will be happy
> > to send you). Dave
> >
> > On Mar 17, 2009, at 11:37 AM, Peter Carroll wrote:
> >
> >>
> >> > I have been told by a Nikon representative that Zeiss oil could
> >> destroy the Nikon objective lens
> >>
> >> Of course you heard that... Nikon wants you to purchase /their/
> >> consumables, not their competitors! Heh...
> >
> > Dr. David Knecht
> > Department of Molecular and Cell Biology
> > Co-head Flow Cytometry and Confocal Microscopy Facility
> > U-3125
> > 91 N. Eagleville Rd.
> > University of Connecticut
> > Storrs, CT 06269
> > 860-486-2200
> > 860-486-4331 (fax)
> >
> >
> >
> >
>
> --
> View this message in context:
> http://n2.nabble.com/Immersion-Oil-tp2491973p2492239.html
> Sent from the Confocal Microscopy List mailing list archive at Nabble.com.
>

--
Kurt Thorn, PhD
Director, Nikon Imaging Center
University of California San Francisco

UCSF MC 2140
Genentech Hall Room S252
600 16th St.
San Francisco, CA 94158-2517

http://nic.ucsf.edu
phone 415.514.9709
fax 415.514.4300

Boswell, Carl A - (cboswell)

Re: Immersion Oil

In reply to this post by Knecht, David

The stories are real, at least with respect to the miscibility of different oils. While one might think that oil is oil, two different immersion oils together can behave like oil and water. That is why I insist that samples first viewed on some other microscope be scrupulously cleaned of whatever oil was used before applying oil used with our Core systems.

As for the issue of one manufacture's oil eating away another manufacture's lens, I'd consider the source of that information carefully. I also use Cargille oil on multiple sytems and have no complaints.

Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-954-7053
FAX 520-621-3709

----- Original Message -----

From: [hidden email]

To: [hidden email]

Sent: Tuesday, March 17, 2009 6:56 AM

Subject: Immersion Oil

We now have Nikon, Zeiss and Leica microscopes in our facility. Is the problem of cross-contamination of immersion oil real or hypothetical? Is it possible to use a single type of immersion oil for all, so that we don't have to worry about moving samples between microscopes? Dave

Dr. David Knecht

Department of Molecular and Cell Biology

Co-head Flow Cytometry and Confocal Microscopy Facility

U-3125

91 N. Eagleville Rd.

University of Connecticut

Storrs, CT 06269

860-486-2200

860-486-4331 (fax)

Csúcs Gábor

Re: Immersion Oil

In reply to this post by Paul Maddox

We are using Zeiss oil on Leica/Zeiss and Olympus objectives for more
then 4 years - without any problems. With Nikon we have no experience.

Cheers Gabor

mmodel

Re: Immersion Oil

In reply to this post by Boswell, Carl A - (cboswell)

I agree with Karl

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Carl Boswell
Sent: Tuesday, March 17, 2009 1:44 PM
To: [hidden email]
Subject: Re: Immersion Oil

Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-954-7053
FAX 520-621-3709

----- Original Message -----

From: [hidden email]

To: [hidden email]

Sent: Tuesday, March 17, 2009 6:56 AM

Subject: Immersion Oil

We now have Nikon, Zeiss and Leica microscopes in our facility. Is the problem of cross-contamination of immersion oil real or hypothetical? Is it possible to use a single type of immersion oil for all, so that we don't have to worry about moving samples between microscopes? Dave

Dr. David Knecht

Department of Molecular and Cell Biology

Co-head Flow Cytometry and Confocal Microscopy Facility

U-3125

91 N. Eagleville Rd.

University of Connecticut

Storrs, CT 06269

860-486-2200

860-486-4331 (fax)

Ning Y. Chan

Re: Immersion Oil

In reply to this post by Kurt Thorn

I simulated the performance of high NA objectives using both vector diffraction theory plus the Gibson and Lanni aberration model and objective lens prescriptions from the patents of various companies (Nikon, Olympus). I found that spherical aberrations are sensitive to an index variation of ~0.002 for oil and ~0.003 for the cover glass.
We had measured the dispersion of a few oils and found their Abbe numbers to be different - confirming Kurt's suspicion.

Ning

====================================
Ning Y. Chan
Nanotechnology Measurements Division
Agilent Technologies, Inc.
5301 Stevens Creek Blvd
Santa Clara, CA 95052
phone: 408 553 3123
FAX: 408 246 5925
[hidden email]

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Kurt Thorn
Sent: Tuesday, March 17, 2009 10:11 AM
To: [hidden email]
Subject: Re: Immersion Oil

One thing I have heard is that different oils have different
dispersions, so you may introduce chromatic aberration if you use oils
from different vendors. I would do the experiment with multicolored
beads and look for axial shifts in the different channels before
deciding to switch oils.

Kurt

Maddox Paul wrote:

>
> It's an easy test to prove that the last (third) decimal place makes a
> reasonable difference. Using the Cargille test kit (range of ~20 RI
> around 1.515 step by 0.001), you can acquired PSFs with the different
> oils and just take a look at the x,z projections. You'll see a pretty
> dramatic shift in spherical aberration, even on small steps (ok, 0.001
> is pretty small change, but 0.002 change is measurable for sure). It
> gets even more interesting (dare I say fun) if you mount the beads in
> some low percentage agar so that you get beads at random distances
> from the coverglass! I did this one weekend and recommend it if you
> need a distraction (from grants for instance...).
>
> As for mixing oils, I always thought the big worry was that different
> brands might not be miscible and therefore you could introduce a new
> RI boundary, causing aberrations. I have never tested, or in fact seen
> this demonstrated (on purpose or otherwise). Nonetheless, I will
> continue to not mix my oils.
>
> Happy imaging,
>
> Paul
>
> Paul S. Maddox, PhD
> Assistant Professor
> Institute for Research in Immunology and Cancer
> Dept of Pathology and Cell Biol, U. de Montreal
> P.O. Box 6128, Station Centre-Ville
> Montréal QC H3C 3J7
> CANADA
>
> Courier:
> 2900, boulevard Édouard-Montpetit
> Pavillon Marcelle-Coutu, Quai 20
> Montreal QC H3T 1J4
> CANADA
>
> [hidden email] <mailto:[hidden email]>
> Ph: 514-343-7894
> Fax: 514-343-6843
>
> ------------------------------------------------------------------------
>
> *From:* Confocal Microscopy List
> [mailto:[hidden email]] *On Behalf Of *Craig Brideau
> *Sent:* Tuesday, March 17, 2009 12:24 PM
> *To:* [hidden email]
> *Subject:* Re: Immersion Oil
>
> When you are down to the third decimal place it's not going to make a
> huge difference*. You might want to try and see if you observe any
> difference between oil, but I suspect you won't see much change.
>
> Craig
>
> *unless you are talking about fiber optics or some optoelectronics...
>
> On Tue, Mar 17, 2009 at 9:58 AM, stu_the_flat <[hidden email]
> <mailto:[hidden email]>> wrote:
>
> Isn't there a 0.002 difference in the diffraction between some of the
> immersion oils?
>
> Off the top of my head
>
> Olympus Oil = 1.515
> Ziess Oil = 1.515
> Nikon Oil = 1.513
>
> I don't think it make the world of difference but as I am interested
> in PSFs
> I always match the oil the microscope.
>
> Stuart
>
>
>
>
>
> David Knecht-charter wrote:
> >
> > Being a natural skeptic, that has been my presumption as well. I
> > haven't talked to anyone who has actually seen mixed oils gum up their
> > scope or dissolve lens cement (if you believe that last one, I have a
> > few million dollars in my uncle's account in Africa I will be happy
> > to send you). Dave
> >
> > On Mar 17, 2009, at 11:37 AM, Peter Carroll wrote:
> >
> >>
> >> > I have been told by a Nikon representative that Zeiss oil could
> >> destroy the Nikon objective lens
> >>
> >> Of course you heard that... Nikon wants you to purchase /their/
> >> consumables, not their competitors! Heh...
> >
> > Dr. David Knecht
> > Department of Molecular and Cell Biology
> > Co-head Flow Cytometry and Confocal Microscopy Facility
> > U-3125
> > 91 N. Eagleville Rd.
> > University of Connecticut
> > Storrs, CT 06269
> > 860-486-2200
> > 860-486-4331 (fax)
> >
> >
> >
> >
>
> --
> View this message in context:
> http://n2.nabble.com/Immersion-Oil-tp2491973p2492239.html
> Sent from the Confocal Microscopy List mailing list archive at Nabble.com.
>

Glen MacDonald-2

Re: Immersion Oil

In reply to this post by Boswell, Carl A - (cboswell)

We also use Cargille on all of our microscopes and insist that
specimens are cleaned of other oils before viewing on our equipment.
Several years ago at the 3D of Microscopy of Living Cells course,
someone ran out of Nikon oil and borrowed some Zeiss oil. the
result was a thick mucous-like substance that took hours to clean from
the objective. Maybe the oils have been re-formulated since then and
behave differently. Mixing the likely suspects to observe the results
could be an interesting science projectt. But, as always, be careful
when exchanging fluids.

Regards,
Glen
On Mar 17, 2009, at 10:43 AM, Carl Boswell wrote:

> The stories are real, at least with respect to the miscibility of
> different oils. While one might think that oil is oil, two
> different immersion oils together can behave like oil and water.
> That is why I insist that samples first viewed on some other
> microscope be scrupulously cleaned of whatever oil was used before
> applying oil used with our Core systems.
>
> As for the issue of one manufacture's oil eating away another
> manufacture's lens, I'd consider the source of that information
> carefully. I also use Cargille oil on multiple sytems and have no
> complaints.
> C
>
> Carl A. Boswell, Ph.D.
> Molecular and Cellular Biology
> University of Arizona
> 520-954-7053
> FAX 520-621-3709
> ----- Original Message -----
> From: David Knecht
> To: [hidden email]
> Sent: Tuesday, March 17, 2009 6:56 AM
> Subject: Immersion Oil
>
> We now have Nikon, Zeiss and Leica microscopes in our facility. Is
> the problem of cross-contamination of immersion oil real or
> hypothetical? Is it possible to use a single type of immersion oil
> for all, so that we don't have to worry about moving samples between
> microscopes? Dave
>
> Dr. David Knecht
> Department of Molecular and Cell Biology
> Co-head Flow Cytometry and Confocal Microscopy Facility
> U-3125
> 91 N. Eagleville Rd.
> University of Connecticut
> Storrs, CT 06269
> 860-486-2200
> 860-486-4331 (fax)
>
>
>

Monique Vasseur

Re: Immersion Oil

In reply to this post by Peter Carroll

He said the Zeiss oils have a small amount of solvent in it in order to disolved old dried Zeiss oils. Can someone comment on this if this is true?

Monique Vasseur
tél. (514) 343-6111 poste 5148
-----Message d'origine-----
De : Confocal Microscopy List [mailto:[hidden email]] De la part de Peter Carroll
Envoyé : 17 mars 2009 11:38
À : [hidden email]
Objet : Re: Immersion Oil

> I have been told by a Nikon representative that Zeiss oil could
destroy the Nikon objective lens

Of course you heard that... Nikon wants you to purchase /their/
consumables, not their competitors! Heh...

Peter Carroll

Re: Immersion Oil

In reply to this post by Glen MacDonald-2

> someone ran out of Nikon oil and borrowed some Zeiss oil. the
result was a thick mucous-like substance that took hours to clean from
the objective

Just for the record, I just mixed these two (Zeiss Immersol 518n and
Nikon Immersion Oil Type A) and didn't observe anything remotely out of
the ordinary, especially in regards to "a thick mucous-like substance"
or anything that would damage optics, so...

>

Glen MacDonald-2

Re: Immersion Oil

Dear Peter
Admittedly, I was in the lab next door and not present when it
happened, only watched the cleaning process afterwards. It wasn't
damaging, just took a long time to remove with lens cleaner and
alcohol. Maybe there has been some reformulation of oils over the
years, or something else actually on the lens, like chamber grease.
Thanks for doing the experiment with current oils.

Regards,
Glen
On Mar 17, 2009, at 12:07 PM, Peter Carroll wrote:

> > someone ran out of Nikon oil and borrowed some Zeiss oil. the
> result was a thick mucous-like substance that took hours to clean
> from the objective
>
> Just for the record, I just mixed these two (Zeiss Immersol 518n and
> Nikon Immersion Oil Type A) and didn't observe anything remotely out
> of the ordinary, especially in regards to "a thick mucous-like
> substance" or anything that would damage optics, so...
>
>
>>

Peter Carroll

Re: Immersion Oil

I haven't had a chance to test the immersion oil cocktail out on the
confocal at length, so I would most definitely be curious if anyone else
has any experiences or other observations with this...

Glen MacDonald wrote:

> Dear Peter
> Admittedly, I was in the lab next door and not present when it
> happened, only watched the cleaning process afterwards. It wasn't
> damaging, just took a long time to remove with lens cleaner and
> alcohol. Maybe there has been some reformulation of oils over the
> years, or something else actually on the lens, like chamber grease.
> Thanks for doing the experiment with current oils.
>
> Regards,
> Glen
> On Mar 17, 2009, at 12:07 PM, Peter Carroll wrote:
>
>> > someone ran out of Nikon oil and borrowed some Zeiss oil. the
>> result was a thick mucous-like substance that took hours to clean
>> from the objective
>>
>> Just for the record, I just mixed these two (Zeiss Immersol 518n and
>> Nikon Immersion Oil Type A) and didn't observe anything remotely out
>> of the ordinary, especially in regards to "a thick mucous-like
>> substance" or anything that would damage optics, so...
>>
>>
>>>
>
>

S. Brunet

Re: Immersion Oil

In reply to this post by Knecht, David

Hello:

Don't mix Zeiss Immersol 518F and Cargille Type 37 immersion oils (the SOP I
wrote was clear on this!). I tried cleaning it but I had no luck. If I put a
drop of Zeiss oil on that objective and put a coverslip on, I can clearly see a
region which does not mix when I image the oil area/volume under a different
microscope!

I clean all objectives between batches of Zeiss oil just in case.

Bye!
Sophie
____________________________________________________
Sophie M. K. Brunet, Ph. D.
Research Officer
Optical Spectroscopy, Laser Systems and Applications
[hidden email]
306-966-1719 (office) 306-966-1702 (fax)
____________________________________________________
Saskatchewan Structural Sciences Centre
University of Saskatchewan
Thorvaldson Bldg.
110 Science Place
Saskatoon, Sk S7N 5C9
____________________________________________________

Quoting David Knecht <[hidden email]>:

> We now have Nikon, Zeiss and Leica microscopes in our facility. Is
> the problem of cross-contamination of immersion oil real or
> hypothetical? Is it possible to use a single type of immersion oil
> for all, so that we don't have to worry about moving samples between
> microscopes? Dave
>
> Dr. David Knecht
> Department of Molecular and Cell Biology
> Co-head Flow Cytometry and Confocal Microscopy Facility
> U-3125
> 91 N. Eagleville Rd.
> University of Connecticut
> Storrs, CT 06269
> 860-486-2200
> 860-486-4331 (fax)
>
>
>

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