Immersion oil with various refractive index

[Aleksander Szczurek]

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Hi All,
I was wondering if anybody of you knows where to purchase immersion oils of
different refractive index (RI). We are currently in need of immersion oils
with RI in the following range: 1.510, 1.512, 1.514, 1.516. I would be very
grateful for any hints and experience from your side.

greetings
Aleksander Szczurek

[Boehm, Ulrike (NIH/NCI) [F]]

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Hi Aleksander,

I used to order immersion oil from Cargille (http://www.cargille.com/immerliq.shtml ).

Best regards,

Ulrike

____________________

Ulrike Boehm, Ph.D.
Laboratory of Receptor Biology & Gene Expression
NCI, National Institutes of Health
41 Medlars Drive
Building 41, Room B303
Bethesda, MD-20892, United States
p (office): [phone]
p (lab): [phone]
f: [phone]
http://ulrikeboehm.org/ 
[hidden email]



-----Original Message-----
From: Aleksander Szczurek [mailto:[hidden email]]
Sent: Friday, September 22, 2017 5:17 PM
To: [hidden email]
Subject: Immersion oil with various refractive index

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Hi All,
I was wondering if anybody of you knows where to purchase immersion oils of different refractive index (RI). We are currently in need of immersion oils with RI in the following range: 1.510, 1.512, 1.514, 1.516. I would be very grateful for any hints and experience from your side.

greetings
Aleksander Sz

czurek

[Eric Marino]

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You can try …

http://www.cargille.com/immeroil.shtml?gclid=CjwKEAjw6ZLOBRCLyNXAwfCPmlQSJADHPIJbzGBNklUsGHXOfVbns2QHJ1BcYsF5e6N8qenm0gaHARoC-ODw_wcB

Eric Marino
[hidden email]

On 9/22/17, 5:16 PM, "Confocal Microscopy List on behalf of Aleksander Szczurek" <[hidden email] on behalf of [hidden email]> wrote:

    *****
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    *****
   
    Hi All,
    I was wondering if anybody of you knows where to purchase immersion oils of
    different refractive index (RI). We are currently in need of immersion oils
    with RI in the following range: 1.510, 1.512, 1.514, 1.516. I would be very
    grateful for any hints and experience from your side.
   
    greetings
    Aleksander Szczurek
   

[Craig Brideau]

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Cargille sells a range of various index oils. According to an old Cargille
brochure I happen to have, 'Series A' oils go from 1.460 to 1.640 in steps
of 0.002! A full set used to run you ~$430 USD back in 2012, and half sets
were about half the price.

Craig

On Fri, Sep 22, 2017 at 3:16 PM, Aleksander Szczurek <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi All,
> I was wondering if anybody of you knows where to purchase immersion oils of
> different refractive index (RI). We are currently in need of immersion oils
> with RI in the following range: 1.510, 1.512, 1.514, 1.516. I would be very
> grateful for any hints and experience from your side.
>
> greetings
> Aleksander Szczurek
>

[David Elliott]

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As a cheaper option you could also try thiodiethanol mixed with water, as described in the following article:

https://biochimie.umontreal.ca/wp-content/uploads/sites/37/2012/08/Thiodiethanol-new-water-mounting-media-2007.pdf

[Benjamin Smith]

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Wow, that thiodiethanol paper is awesome, thanks for the heads-up!
Although, I can't imaging it smells too great (and the SDS for it seems to
agree).

On Fri, Sep 22, 2017 at 3:29 PM, David Elliott <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> As a cheaper option you could also try thiodiethanol mixed with water, as
> described in the following article:
>
> https://biochimie.umontreal.ca/wp-content/uploads/sites/
> 37/2012/08/Thiodiethanol-new-water-mounting-media-2007.pdf
>

--
Benjamin E. Smith, Ph. D.
Imaging Specialist, Vision Science
University of California, Berkeley
195 Life Sciences Addition
Berkeley, CA  94720-3200
Tel  [phone]
Fax [phone]
e-mail: [hidden email]
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[Zdenek Svindrych-2]

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Benjamin,
don't worry, it's not a thiol, it does not smell at all. 

But it's a useful stuff for sure. About the price of glycerol, but less
viscous and higher RI. Not hygroscopic. I use it a lot for index matching,
but haven't actually used it as a substitute for immersion oil. You can
dilute it with water to the required refractive index, but the water will
evaporate over time and the RI will increase. I would mix it with something
with lower water pressure, such as propylene glycol (but maybe something
less hygroscopic and less viscous)...

Best, zdenek

--
Zdenek Svindrych, Ph.D.
W.M. Keck Center for Cellular Imaging (PLSB 003)
Department of Biology,University of Virginia
409 McCormick Rd, Charlottesville, VA-22904
http://www.kcci.virginia.edu/
tel: [phone]

---------- Původní e-mail ----------
Od: Benjamin E Smith <[hidden email]>
Komu: [hidden email]
Datum: 22. 9. 2017 20:30:12
Předmět: Re: Immersion oil with various refractive index
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Wow, that thiodiethanol paper is awesome, thanks for the heads-up!
Although, I can't imaging it smells too great (and the SDS for it seems to
agree).

On Fri, Sep 22, 2017 at 3:29 PM, David Elliott <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> As a cheaper option you could also try thiodiethanol mixed with water, as
> described in the following article:
>
> https://biochimie.umontreal.ca/wp-content/uploads/sites/
> 37/2012/08/Thiodiethanol-new-water-mounting-media-2007.pdf
>

--
Benjamin E. Smith, Ph. D.
Imaging Specialist, Vision Science
University of California, Berkeley
195 Life Sciences Addition
Berkeley, CA 94720-3200
Tel [phone]
Fax [phone]
e-mail: [hidden email]
http://vision.berkeley.edu/?page_id=5635 <http://vision.berkeley.edu/>
"

[Tim Feinstein]

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Agreed that TDE is a great way to mix up a non-hardening mount with whatever RI you need.  It has no smell.  

Ethanedithiol, on the other hand, smells like the end of the world.  It's like beta mercaptoethanol's meaner big brother.  They don't warn you about that stuff in the papers that describe tetracysteine motif labeling.  

Best,


T

Timothy Feinstein, Ph.D.
Research Scientist
University of Pittsburgh Department of Developmental Biology

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of [hidden email]
Sent: Friday, September 22, 2017 8:48 PM
To: [hidden email]
Subject: Re: Immersion oil with various refractive index

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Benjamin,
don't worry, it's not a thiol, it does not smell at all. 

But it's a useful stuff for sure. About the price of glycerol, but less viscous and higher RI. Not hygroscopic. I use it a lot for index matching, but haven't actually used it as a substitute for immersion oil. You can dilute it with water to the required refractive index, but the water will evaporate over time and the RI will increase. I would mix it with something with lower water pressure, such as propylene glycol (but maybe something less hygroscopic and less viscous)...

Best, zdenek

--
Zdenek Svindrych, Ph.D.
W.M. Keck Center for Cellular Imaging (PLSB 003) Department of Biology,University of Virginia
409 McCormick Rd, Charlottesville, VA-22904
https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.kcci.virginia.edu%2F&data=01%7C01%7Ctnf8%40PITT.EDU%7Cb2aab68a7b9c4d11b05608d5021ccc09%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1&sdata=eT%2Fh1ThyJXMeOUh8KEoUD4CBxkHG%2FpDq1tBxoWFUTUE%3D&reserved=0
tel: [phone]

---------- Původní e-mail ----------
Od: Benjamin E Smith <[hidden email]>
Komu: [hidden email]
Datum: 22. 9. 2017 20:30:12
Předmět: Re: Immersion oil with various refractive index
"*****
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*****

Wow, that thiodiethanol paper is awesome, thanks for the heads-up!
Although, I can't imaging it smells too great (and the SDS for it seems to agree).

On Fri, Sep 22, 2017 at 3:29 PM, David Elliott <[hidden email]>
wrote:

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> https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&data=01%7C01%7Ctnf8%40PITT.EDU%7Cb2aab68a7b9c4d11b05608d5021ccc09%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1&sdata=R4ExCA0hu0QBvjAmJD%2FxMby3GmKmqgPIXKU%2FhpX%2BHaI%3D&reserved=0 and include the link in your posting.
> *****
>
> As a cheaper option you could also try thiodiethanol mixed with water,
> as described in the following article:
>
> https://na01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fbioch
> imie.umontreal.ca%2Fwp-content%2Fuploads%2Fsites%2F&data=01%7C01%7Ctnf
> 8%40PITT.EDU%7Cb2aab68a7b9c4d11b05608d5021ccc09%7C9ef9f489e0a04eeb87cc
> 3a526112fd0d%7C1&sdata=tDT4wuI2UrFlMB0lIWExIaJXiW7mOwYxzfS2WKMHvC0%3D&
> reserved=0 37/2012/08/Thiodiethanol-new-water-mounting-media-2007.pdf
>

--
Benjamin E. Smith, Ph. D.
Imaging Specialist, Vision Science
University of California, Berkeley
195 Life Sciences Addition
Berkeley, CA 94720-3200
Tel [phone]
Fax [phone]
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"

[Barbara Foster]

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Hi, Alexander,

Try The McCrone Group (www.mccrone.com).  They have carried RI
standards for many years and are a highly trusted source.

Also, remember that RI is very wavelength and temperature
dependent.  To be accurate to the third decimal place, as you have
indicated in your message, use a narrowband filter over the light
source and, preferrably, a temperature controlled stage.  Most RI's
are given nD (sodium D line) and 25o C.

Hope this was helpful.  (Caveat: No commercial interest)
Barbara Foster, President & Chief Consultant
Microscopy/Microscopy Education  ... "Education, not JustTraining"
7101 Royal Glen Trail, Suite A  - McKinney, TX 75070
P: 972-924-5310 W: www.MicroscopyEducation.com
Microscopy/Microscopy Education is a division of The Microscopy &
Imaging Place, Inc.
At 09:57 AM 9/22/2017, you wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>Post images on http://www.imgur.com and include the link in your posting.
>*****
>
>Hi All,
>I was wondering if anybody of you knows where to purchase immersion oils of
>different refractive index (RI). We are currently in need of immersion oils
>with RI in the following range: 1.510, 1.512, 1.514, 1.516. I would be very
>grateful for any hints and experience from your side.
>
>greetings
>Aleksander Szczurek

[Aleksander Szczurek]

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Dear All,
thank you very much for a valuable input. I knew this is going to be a good
place to ask :-)
Wish you all the best in your experiments!
Aleks

2017-09-27 22:11 GMT+02:00 Barbara Foster <[hidden email]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi, Alexander,
>
> Try The McCrone Group (www.mccrone.com).  They have carried RI standards
> for many years and are a highly trusted source.
>
> Also, remember that RI is very wavelength and temperature dependent.  To
> be accurate to the third decimal place, as you have indicated in your
> message, use a narrowband filter over the light source and, preferrably, a
> temperature controlled stage.  Most RI's are given nD (sodium D line) and
> 25o C.
>
> Hope this was helpful.  (Caveat: No commercial interest)
> Barbara Foster, President & Chief Consultant
> Microscopy/Microscopy Education  ... "Education, not JustTraining"
> 7101 Royal Glen Trail, Suite A  - McKinney, TX 75070
> P: 972-924-5310 W: www.MicroscopyEducation.com
> Microscopy/Microscopy Education is a division of The Microscopy & Imaging
> Place, Inc.
> At 09:57 AM 9/22/2017, you wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Hi All,
>> I was wondering if anybody of you knows where to purchase immersion oils
>> of
>> different refractive index (RI). We are currently in need of immersion
>> oils
>> with RI in the following range: 1.510, 1.512, 1.514, 1.516. I would be
>> very
>> grateful for any hints and experience from your side.
>>
>> greetings
>> Aleksander Szczurek
>>
>