Infra-Red Fluorescent protein in C. elegans

[lgelman]

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Hi everybody,

Has anyone ever expressed a fluorescent protein emitting in the Far-Red in C. elegans?

Many thanks in advance for your help and very best regards,

Laurent.

Laurent Gelman
Facility for Advanced Imaging and Microscopy
Head Light Microscopy
Friedrich Miescher Institute for Biomedical Research
Maulbeerstrasse 66
4058 basel
Switzerland
[phone]

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Hi Laurent,

I can't help you with the C. elegans part (there, I have only expressed eGFP & eYFP), but in mouse & rat brain tissue we have had significant success using mNeptune2 and mCardinal. Both were comparatively stable & bright, even when using STED microscopy. (Still no comparison to FPs in the green, though.)
mKate2 and Tag-RFP worked quite well, too.

Sorry that is all I can contribute!

Nicolai

>>>>>>>>>><<<<<<<<>>>>>>>>>><<<<<<<<<<>>>>>>>>>><<<<<<<<<<
Dr. Nicolai T. Urban
Max Planck Florida Institute
Jupiter, 33458 FL, USA


-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Gelman, Laurent
Sent: Freitag, 24. August 2018 09:52
To: [hidden email]
Subject: Infra-Red Fluorescent protein in C. elegans

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Hi everybody,

Has anyone ever expressed a fluorescent protein emitting in the Far-Red in C. elegans?

Many thanks in advance for your help and very best regards,

Laurent.

Laurent Gelman
Facility for Advanced Imaging and Microscopy Head Light Microscopy Friedrich Miescher Institute for Biomedical Research Maulbeerstrasse 66
4058 basel
Switzerland
[phone]

[anuj sharma]

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Hi Laurent,

We are using mNeptune expressing pan-neuronally in C. elegans,
Will that be useful?

Best
Anuj

On Fri, Aug 24, 2018 at 10:07 AM Gelman, Laurent <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi everybody,
>
> Has anyone ever expressed a fluorescent protein emitting in the Far-Red in
> C. elegans?
>
> Many thanks in advance for your help and very best regards,
>
> Laurent.
>
> Laurent Gelman
> Facility for Advanced Imaging and Microscopy
> Head Light Microscopy
> Friedrich Miescher Institute for Biomedical Research
> Maulbeerstrasse 66
> 4058 basel
> Switzerland
> [phone]
>

--
*Anuj Kumar Sharma, Ph.D.*
Staff Scientist
Department of Physics, Princeton University
[hidden email]; [hidden email]

[lgelman]

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Dear Anuj,
Sure, thank a lot for the tip!
Best regards,
Laurent.

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Anuj Sharma
Sent: Monday, August 27, 2018 16:01
To: [hidden email]
Subject: Re: Infra-Red Fluorescent protein in C. elegans

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Hi Laurent,

We are using mNeptune expressing pan-neuronally in C. elegans, Will that be useful?

Best
Anuj

On Fri, Aug 24, 2018 at 10:07 AM Gelman, Laurent <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi everybody,
>
> Has anyone ever expressed a fluorescent protein emitting in the
> Far-Red in C. elegans?
>
> Many thanks in advance for your help and very best regards,
>
> Laurent.
>
> Laurent Gelman
> Facility for Advanced Imaging and Microscopy Head Light Microscopy
> Friedrich Miescher Institute for Biomedical Research Maulbeerstrasse
> 66
> 4058 basel
> Switzerland
> [phone]
>

--
*Anuj Kumar Sharma, Ph.D.*
Staff Scientist
Department of Physics, Princeton University [hidden email]; [hidden email]

[Douglas Richardson]

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We've successfully used iRFP670 in crickets and zebrafish.  It's not super
bright, but was fine to mark nuclei in an H2B fusion.

-Doug

On Wed, Aug 29, 2018 at 11:47 AM Gelman, Laurent <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Anuj,
> Sure, thank a lot for the tip!
> Best regards,
> Laurent.
>
> -----Original Message-----
> From: Confocal Microscopy List <[hidden email]> On
> Behalf Of Anuj Sharma
> Sent: Monday, August 27, 2018 16:01
> To: [hidden email]
> Subject: Re: Infra-Red Fluorescent protein in C. elegans
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Laurent,
>
> We are using mNeptune expressing pan-neuronally in C. elegans, Will that
> be useful?
>
> Best
> Anuj
>
> On Fri, Aug 24, 2018 at 10:07 AM Gelman, Laurent <[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Hi everybody,
> >
> > Has anyone ever expressed a fluorescent protein emitting in the
> > Far-Red in C. elegans?
> >
> > Many thanks in advance for your help and very best regards,
> >
> > Laurent.
> >
> > Laurent Gelman
> > Facility for Advanced Imaging and Microscopy Head Light Microscopy
> > Friedrich Miescher Institute for Biomedical Research Maulbeerstrasse
> > 66
> > 4058 basel
> > Switzerland
> > [phone]
> >
>
>
> --
> *Anuj Kumar Sharma, Ph.D.*
> Staff Scientist
> Department of Physics, Princeton University [hidden email];
> [hidden email]
>