*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi, This is my first email to this list. Looks to be a good resource. I am wondering if anyone has a recommendation for a good microscope course. Specifically, I am interested in taking a course which will focus primarily on assembling microscopes on a table from existing optical and mechanical parts. I am not looking for a course where you learn how to use very expensive microscopes made by others, or where you learn about the latest microscope designs. My web searches have yielded many theory and hands-on courses, but none that seem to focus on actually learning by building. I've read many books and articles, but at this point I think I need actual projects (e.g. build a microscope to image X) rather than repetition of the same theory I've heard many times before. Anyone know of any courses out there for building microscopes? Maybe Thor or one of the existing parts suppliers puts one on? thanks, nikhil Blog: http://superfacts.org Webapps: http://wormweb.org Android apps: http://android.superfacts.org |
Periasamy, Ammasi (ap3t) |
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I am not sure which part of the world you are from... I teach a microscopy course during fall semester. As a part of course the students has to assemble the microscope and align it that includes Koehler and epi-fluorescence illumination. All the parts of the microscope will be disassembled before the student enter the room. This course is designed for the biologists. In case if you are not from UVA...you can come to our annual workshop on FRET microscopy, I can ask you to do this project. Not sure you are interested in FRET and FLIM. Dr. Ammasi Periasamy Professor & Center Director W.M. Keck Center for Cellular Imaging (KCCI) (University of Virginia Imaging Center) Mail or FedEx or UPS: Keck Center for Cellullar Imaging University of Virginia Biology, Gilmer Hall, 485 McCormick Rd. Charlottesville, VA 22904, USA Office Location: Physical and Life Sciences Building (PLSB-B005), White Head Rd., Voice: 434-243-7602 (Office); 982-4869 (lab) Fax:434-982-5210; Email:[hidden email] http://www.kcci.virginia.edu/contact/peri.php ************************ 13th Annual Workshop on FRET & FLIM Microscopy, March 10-15, 2014 http://www.kcci.virginia.edu/workshop/workshop2014/index.php ************************* -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Nikhil Bhatla Sent: Wednesday, June 12, 2013 12:35 AM To: [hidden email] Subject: Lab class to build microscopes ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi, This is my first email to this list. Looks to be a good resource. I am wondering if anyone has a recommendation for a good microscope course. Specifically, I am interested in taking a course which will focus primarily on assembling microscopes on a table from existing optical and mechanical parts. I am not looking for a course where you learn how to use very expensive microscopes made by others, or where you learn about the latest microscope designs. My web searches have yielded many theory and hands-on courses, but none that seem to focus on actually learning by building. I've read many books and articles, but at this point I think I need actual projects (e.g. build a microscope to image X) rather than repetition of the same theory I've heard many times before. Anyone know of any courses out there for building microscopes? Maybe Thor or one of the existing parts suppliers puts one on? thanks, nikhil Blog: http://superfacts.org Webapps: http://wormweb.org Android apps: http://android.superfacts.org |
Csúcs Gábor-2 |
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear Ammasi, Disassembling and re-assembling a microscope sounds like a nice student project. Can you share with us what microscope (manufacturer) you use for this exercise? Thanks and Greetings Gabor Light Microscopy and Screening Centre ETH Zurich, HPM G41 Schafmattstrasse 18 CH-8093, Zurich Switzerland Phone: +41 44 633 6221 Mobile: +41 79 7582158 On 6/12/13 3:40 PM, "Periasamy, Ammasi (ap3t)" <[hidden email]> wrote: >***** >To join, leave or search the confocal microscopy listserv, go to: >http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >***** > >I am not sure which part of the world you are from... >I teach a microscopy course during fall semester. As a part of course the >students has to assemble the microscope and align it that includes >Koehler and epi-fluorescence illumination. All the parts of the >microscope will be disassembled before the student enter the room. >This course is designed for the biologists. >In case if you are not from UVA...you can come to our annual workshop on >FRET microscopy, I can ask you to do this project. Not sure you are >interested in FRET and FLIM. > > >Dr. Ammasi Periasamy >Professor & Center Director >W.M. Keck Center for Cellular Imaging (KCCI) >(University of Virginia Imaging Center) >Mail or FedEx or UPS: >Keck Center for Cellullar Imaging >University of Virginia >Biology, Gilmer Hall, 485 McCormick Rd. >Charlottesville, VA 22904, USA >Office Location: >Physical and Life Sciences Building (PLSB-B005), White Head Rd., >Voice: 434-243-7602 (Office); 982-4869 (lab) >Fax:434-982-5210; Email:[hidden email] >http://www.kcci.virginia.edu/contact/peri.php >************************ >13th Annual Workshop on FRET & FLIM Microscopy, March 10-15, 2014 >http://www.kcci.virginia.edu/workshop/workshop2014/index.php >************************* > > >-----Original Message----- >From: Confocal Microscopy List [mailto:[hidden email]] >On Behalf Of Nikhil Bhatla >Sent: Wednesday, June 12, 2013 12:35 AM >To: [hidden email] >Subject: Lab class to build microscopes > >***** >To join, leave or search the confocal microscopy listserv, go to: >http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >***** > >Hi, > >This is my first email to this list. Looks to be a good resource. > >I am wondering if anyone has a recommendation for a good microscope >course. Specifically, I am interested in taking a course which will >focus primarily on assembling microscopes on a table from existing >optical and mechanical parts. I am not looking for a course where you >learn how to use very expensive microscopes made by others, or where you >learn about the latest microscope designs. My web searches have yielded >many theory and hands-on courses, but none that seem to focus on actually >learning by building. I've read many books and articles, but at this >point I think I need actual projects (e.g. build a microscope to image X) >rather than repetition of the same theory I've heard many times before. > >Anyone know of any courses out there for building microscopes? Maybe >Thor or one of the existing parts suppliers puts one on? > >thanks, >nikhil > >Blog: http://superfacts.org >Webapps: http://wormweb.org >Android apps: http://android.superfacts.org |
Periasamy, Ammasi (ap3t) |
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** You can use any commercially available microscopes. I am using Nikon TE300. As you know when 12-15 students work on this scope..., each year before the semester we have to service this microscope. Hope this helps. Ammasi -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Csúcs Gábor Sent: Wednesday, June 12, 2013 10:25 AM To: [hidden email] Subject: Re: Lab class to build microscopes ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear Ammasi, Disassembling and re-assembling a microscope sounds like a nice student project. Can you share with us what microscope (manufacturer) you use for this exercise? Thanks and Greetings Gabor Light Microscopy and Screening Centre ETH Zurich, HPM G41 Schafmattstrasse 18 CH-8093, Zurich Switzerland Phone: +41 44 633 6221 Mobile: +41 79 7582158 On 6/12/13 3:40 PM, "Periasamy, Ammasi (ap3t)" <[hidden email]> wrote: >***** >To join, leave or search the confocal microscopy listserv, go to: >http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >***** > >I am not sure which part of the world you are from... >I teach a microscopy course during fall semester. As a part of course >the students has to assemble the microscope and align it that includes >Koehler and epi-fluorescence illumination. All the parts of the >microscope will be disassembled before the student enter the room. >This course is designed for the biologists. >In case if you are not from UVA...you can come to our annual workshop >on FRET microscopy, I can ask you to do this project. Not sure you are >interested in FRET and FLIM. > > >Dr. Ammasi Periasamy >Professor & Center Director >W.M. Keck Center for Cellular Imaging (KCCI) (University of Virginia >Imaging Center) Mail or FedEx or UPS: >Keck Center for Cellullar Imaging >University of Virginia >Biology, Gilmer Hall, 485 McCormick Rd. >Charlottesville, VA 22904, USA >Office Location: >Physical and Life Sciences Building (PLSB-B005), White Head Rd., >Voice: 434-243-7602 (Office); 982-4869 (lab) Fax:434-982-5210; >Email:[hidden email] http://www.kcci.virginia.edu/contact/peri.php >************************ >13th Annual Workshop on FRET & FLIM Microscopy, March 10-15, 2014 >http://www.kcci.virginia.edu/workshop/workshop2014/index.php >************************* > > >-----Original Message----- >From: Confocal Microscopy List >[mailto:[hidden email]] >On Behalf Of Nikhil Bhatla >Sent: Wednesday, June 12, 2013 12:35 AM >To: [hidden email] >Subject: Lab class to build microscopes > >***** >To join, leave or search the confocal microscopy listserv, go to: >http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >***** > >Hi, > >This is my first email to this list. Looks to be a good resource. > >I am wondering if anyone has a recommendation for a good microscope >course. Specifically, I am interested in taking a course which will >focus primarily on assembling microscopes on a table from existing >optical and mechanical parts. I am not looking for a course where you >learn how to use very expensive microscopes made by others, or where >you learn about the latest microscope designs. My web searches have >yielded many theory and hands-on courses, but none that seem to focus >on actually learning by building. I've read many books and articles, >but at this point I think I need actual projects (e.g. build a >microscope to image X) rather than repetition of the same theory I've heard many times before. > >Anyone know of any courses out there for building microscopes? Maybe >Thor or one of the existing parts suppliers puts one on? > >thanks, >nikhil > >Blog: http://superfacts.org >Webapps: http://wormweb.org >Android apps: http://android.superfacts.org |
Jerry (Gerald) Sedgewick |
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Announcing the 4th Annual Scientific Imaging and Quantization course in Minneapolis, MN, August 26 - 29, 2013. I am giving this 3 - 4-day course as a way to meet a personal mission, which includes: 1. Providing instruction on proper methods for working with images and image stacks. 2. Providing means for making computer-aided quantitation simpler and more consistent with complex biological images. 3. Showing pro-active means for maintaining image integrity throughout the microscope slide to publication process. To learn more about the course, please go to the website quickphotoshop.com, or to http://imagingandanalysis.com/4th_annual_seminars-withmovies.html. You can do that right now. Really. Or read on... The class is for anyone working with scientific imaging using microscopes. The only requirement is that you are able to find files on your hard drive with relative ease, and that you have fairly good hand-eye coordination with the use of a mouse (or your dexterous fingers). You will receive a certificate of completion that can be included in your CV. You can bring along images from which you would like to get measurements. You will learn about the following using methods with techniques you have not likely learned (e.g., how to see if you have saturated pixels when using the Levels tool): Image Integrity: what you can, can't and should do. Calibration of Monitors (NEW!) Calibration devices and techniques (NEW!) Digital Imaging Terms (e.g., gamma, gamut, etc) Opening 12-bit images (10-bit, 14-bit too) in 16-bit space Opening movies and image stacks Photostitching (montaging several images together) Z-projection in Photoshop Cropping, Rotating, Flipping De-Noising methods (averaging single pixel outliers, median filtering, surface blur, etc) Uneven Illumination Correction (flatfield correction, using image itself, vignetting, etc.) Tonal/Color Correction (manual, linear histogram matching, histogram matching, etc.) Co-existence ("colocalization") method Merging Colorizing/DeColorizing Working with movies and lettering/adding symbols Creating Automated Macros (Actions) to apply to a folder of images Creating Figures/Plates: In Illustrator and Photoshop Adding Lettering/Symbols Making Inserts To Outputs: Publication, Laptop Projector, Grants, Posters, Powerpoint How to Use the Image Size function for outputting Sharpening (2 methods) Quantitation: Segmenting (separating out objects of interest from surrounding areas) Segmenting color images Typical Method for Measuring Counts Typical Method for Measuring Morphometry (surface area, lengths, clustering, etc) Methods for Measuring Intensity/Density Intro to Stereology Assistance with your Images Hands on Lab day (day 4) Here's what you will need to bring along: 1) Bring a laptop with (preferably) Photoshop versions CS3 - CS6: earlier versions (Photoshop 6 - CS2) will be OK, but a limited number of functions will not work in Photoshop 6 - CS2. 2. You can bring a Mac or PC. 3. Bring a mouse, unless you are super-dexterous with your fingers. Please write to me with any questions: [hidden email]. All the best! Jerry Sedgewick |
Barbara Foster |
In reply to this post by Nikhil Bhatla
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi, Nikhil Actually, the MME courses take this approach. We start with some basic optical physics then move on to how those basics are applied. In the very early days, especially when we were team teaching with the Royal Microscopical Society and Bob Hoffman (inventor of Hoffman Modulation Contrast), we actually brought small optical benches to the courses (essentially, yardsticks with lenses in holders which would clip onto the yardsticks). However, we found that, with limited time, using the optical physics and experiments on the microscope itself were sufficient. If you'd like to discuss this approach further, please give me a call. Best regards, Barbara Foster, President & Chief Consultant Microscopy/Microscopy Education* www.MicroscopyEducation.com *A subsidiary of The Microscopy & Imaging Place, Inc. 7101 Royal Glen Trail, Suite A McKinney, TX 75070 P: 972-924-5310 F: 214-592-0277 MME is currently scheduling courses for the Fall 2013. Call us today for a free training evaluation. ***CAVEAT: Commercial posting***** At 03:50 PM 6/12/2013, Nikhil Bhatla wrote: >***** >To join, leave or search the confocal microscopy listserv, go to: >http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >***** > >Hi, > >This is my first email to this list. Looks to be a good resource. > >I am wondering if anyone has a recommendation for a good microscope >course. Specifically, I am interested in taking a course which will focus >primarily on assembling microscopes on a table from existing optical and >mechanical parts. I am not looking for a course where you learn how to use >very expensive microscopes made by others, or where you learn about the >latest microscope designs. My web searches have yielded many theory and >hands-on courses, but none that seem to focus on actually learning by >building. I've read many books and articles, but at this point I think I >need actual projects (e.g. build a microscope to image X) rather than >repetition of the same theory I've heard many times before. > >Anyone know of any courses out there for building microscopes? Maybe Thor >or one of the existing parts suppliers puts one on? > >thanks, >nikhil > >Blog: http://superfacts.org >Webapps: http://wormweb.org >Android apps: http://android.superfacts.org |
Craig Brideau |
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Here at the University of Calgary we have a fairly low-level microscope class where we get the students to build all the major sub-systems of a microscope out of discrete parts from Thorlabs and Edmund Optics. It's a lot of fun and the students seem to get a lot out of it. Craig On 2013-06-12 4:21 PM, "Barbara Foster" <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > ***** > > Hi, Nikhil > > Actually, the MME courses take this approach. We start with some basic > optical physics then move on to how those basics are applied. In the very > early days, especially when we were team teaching with the Royal > Microscopical Society and Bob Hoffman (inventor of Hoffman Modulation > Contrast), we actually brought small optical benches to the courses > (essentially, yardsticks with lenses in holders which would clip onto the > yardsticks). However, we found that, with limited time, using the optical > physics and experiments on the microscope itself were sufficient. > > If you'd like to discuss this approach further, please give me a call. > > Best regards, > Barbara Foster, President & Chief Consultant > Microscopy/Microscopy Education* > www.MicroscopyEducation.com > > *A subsidiary of The Microscopy & Imaging Place, Inc. > 7101 Royal Glen Trail, Suite A > McKinney, TX 75070 > P: 972-924-5310 > F: 214-592-0277 > > MME is currently scheduling courses for the Fall 2013. Call us today for a > free training evaluation. > > ***CAVEAT: Commercial posting***** > At 03:50 PM 6/12/2013, Nikhil Bhatla wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> >> ***** >> >> Hi, >> >> This is my first email to this list. Looks to be a good resource. >> >> I am wondering if anyone has a recommendation for a good microscope >> course. Specifically, I am interested in taking a course which will focus >> primarily on assembling microscopes on a table from existing optical and >> mechanical parts. I am not looking for a course where you learn how to >> use >> very expensive microscopes made by others, or where you learn about the >> latest microscope designs. My web searches have yielded many theory and >> hands-on courses, but none that seem to focus on actually learning by >> building. I've read many books and articles, but at this point I think I >> need actual projects (e.g. build a microscope to image X) rather than >> repetition of the same theory I've heard many times before. >> >> Anyone know of any courses out there for building microscopes? Maybe Thor >> or one of the existing parts suppliers puts one on? >> >> thanks, >> nikhil >> >> Blog: http://superfacts.org >> Webapps: http://wormweb.org >> Android apps: http://android.superfacts.org >> > |
Steffen Dietzel |
In reply to this post by Nikhil Bhatla
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Nikhil, in my microscopy course for biology students, to demonstrate the principle, we build a "compound microscope" simply by combining two big plastic lenses, 5x for an objective and a 10x as an eye piece. To find the intermediate image plane, we use a diffusing screen. Once the image is sharp on the diffusing screen and in the eye piece, the diffusing screen is removed. A self-luminous object, i.e. a burning candle, avoids the need for a condenser. There used to be a paper microscope with plastic lenses on the German market, for about 10-15 Euro (ISBN 3935364083) but the assembly took a little long for the course (4-6 h), so I dropped that. It did deliver a surprisingly good quality though. I guess both approaches are not what you had in mind, but for completeness I mention them anyway. If you would be happy to assemble commercial microscopes, maybe you can have a little internship with one of your local microscope company representatives? Steffen On 12.06.2013 06:35, Nikhil Bhatla wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi, > > This is my first email to this list. Looks to be a good resource. > > I am wondering if anyone has a recommendation for a good microscope > course. Specifically, I am interested in taking a course which will focus > primarily on assembling microscopes on a table from existing optical and > mechanical parts. I am not looking for a course where you learn how to use > very expensive microscopes made by others, or where you learn about the > latest microscope designs. My web searches have yielded many theory and > hands-on courses, but none that seem to focus on actually learning by > building. I've read many books and articles, but at this point I think I > need actual projects (e.g. build a microscope to image X) rather than > repetition of the same theory I've heard many times before. > > Anyone know of any courses out there for building microscopes? Maybe Thor > or one of the existing parts suppliers puts one on? > > thanks, > nikhil > > Blog: http://superfacts.org > Webapps: http://wormweb.org > Android apps: http://android.superfacts.org > -- ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität München Walter-Brendel-Zentrum für experimentelle Medizin (WBex) Head of light microscopy Mail room: Marchioninistr. 15, D-81377 München Building location: Marchioninistr. 27, München-Großhadern |
manoj mathew |
In reply to this post by Nikhil Bhatla
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear Nikhil, We run the annual Bangalore Microscopy Course (September 8-15 this year). (http://www.ncbs.res.in/events/microscopy2013/index.html) A range of topics from basic microscopy to super-resolution imaging is covered with strong emphasis on hands on training sessions. The final two days involve a project that participants do in groups. Usually about 6-8 projects are offered. Two of them might be of your interest, 1. Assembling commercial microscopes (Nikon Ti and ASI modular infinity). The systems are disassembled and the participants trained on how to assemble them. Further they automate the system using micromanager software. 2. Building Optical Tweezers. The first part of this project involves assembling a microscope using Thor Labs/Newport optical/opto mechanical components. Subsequently a red laser is introduced onto the sample plane for Tweezing. Best Regards, Manoj -- Manoj Mathew, PhD Technology Consultant Central Imaging and Flow Cytometry Facility Centre for Cellular and Molecular Platforms National Centre for Biological Sciences GKVK Post, Bellary Road Bangalore-560065, India Ph. +91 8067176277 Mob. 09886789049 On Wed, Jun 12, 2013 at 12:35 PM, Nikhil Bhatla <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi, > > This is my first email to this list. Looks to be a good resource. > > I am wondering if anyone has a recommendation for a good microscope > course. Specifically, I am interested in taking a course which will focus > primarily on assembling microscopes on a table from existing optical and > mechanical parts. I am not looking for a course where you learn how to use > very expensive microscopes made by others, or where you learn about the > latest microscope designs. My web searches have yielded many theory and > hands-on courses, but none that seem to focus on actually learning by > building. I've read many books and articles, but at this point I think I > need actual projects (e.g. build a microscope to image X) rather than > repetition of the same theory I've heard many times before. > > Anyone know of any courses out there for building microscopes? Maybe Thor > or one of the existing parts suppliers puts one on? > > thanks, > nikhil > > Blog: http://superfacts.org > Webapps: http://wormweb.org > Android apps: http://android.superfacts.org |
Stad, Robert |
In reply to this post by Nikhil Bhatla
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Nikhil, I can really recommend the EMBO Practical Course in Advanced Optical Microscopy in Plymouth, given by Brad Amos. Here you learn everything from basic to advanced microscopy and optics and one of the practicals is building a microscope on a an optical bench and even putting together a basic laser scanning confocal one. Better check if all of this is still part of the program for next year. Regards, Robert -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Nikhil Bhatla Sent: woensdag 12 juni 2013 06:35 AM To: [hidden email] Subject: Lab class to build microscopes ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi, This is my first email to this list. Looks to be a good resource. I am wondering if anyone has a recommendation for a good microscope course. Specifically, I am interested in taking a course which will focus primarily on assembling microscopes on a table from existing optical and mechanical parts. I am not looking for a course where you learn how to use very expensive microscopes made by others, or where you learn about the latest microscope designs. My web searches have yielded many theory and hands-on courses, but none that seem to focus on actually learning by building. I've read many books and articles, but at this point I think I need actual projects (e.g. build a microscope to image X) rather than repetition of the same theory I've heard many times before. Anyone know of any courses out there for building microscopes? Maybe Thor or one of the existing parts suppliers puts one on? thanks, nikhil Blog: http://superfacts.org Webapps: http://wormweb.org Android apps: http://android.superfacts.org |
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