Laser engine request

[Alan Wainman]

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Hi all,
 
I am a postdoc working with Jordan Raff at the University of Oxford, imaging cell division in Drosophila embryos. We have an old PerkinElmer UltraVIEW Imaging system with a gas laser engine, which we have been struggling to maintain but would like to keep going.
 
We are looking for a PerkinElmer diode laser engine (either MLS1 or MLS2) to add onto the system  - currently using 488, 561 and 440 laser lines. As we hope to keep the system running with Volocity software, it will have to be only a PE laser engine.
 
Would anybody be able to help us out?
 
(If in the UK, we will be happy to dismantle and get the system back to Oxford).

Many thanks in advance for your help.


Alan Wainman

Raff Lab
Sir William Dunn School of Pathology
South Parks Road, Oxford, OX1 3RE
UK
Tel: [phone]

[Owens, James]

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Hi Alan,

You may want to try contacting Alan Tilley of Atlantic Imaging.
https://www.linkedin.com/in/alan-tilley-06947b23/?originalSubdomain=uk

He used to work for PE and has extensive experience of the Ultraview system.

Regards
Jim Owens

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Alan Wainman
Sent: 14 December 2018 17:57
To: [hidden email]
Subject: Laser engine request

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Hi all,
 
I am a postdoc working with Jordan Raff at the University of Oxford, imaging cell division in Drosophila embryos. We have an old PerkinElmer UltraVIEW Imaging system with a gas laser engine, which we have been struggling to maintain but would like to keep going.
 
We are looking for a PerkinElmer diode laser engine (either MLS1 or MLS2) to add onto the system  - currently using 488, 561 and 440 laser lines. As we hope to keep the system running with Volocity software, it will have to be only a PE laser engine.
 
Would anybody be able to help us out?
 
(If in the UK, we will be happy to dismantle and get the system back to Oxford).

Many thanks in advance for your help.


Alan Wainman

Raff Lab
Sir William Dunn School of Pathology
South Parks Road, Oxford, OX1 3RE
UK
Tel: [phone]

[Csúcs Gábor-3]

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Dear Alan,

If I am not mistaken, The PerkinElmer Ultraview used  the Yokogawa CSU22 spinning disk module. This should be possible to control with MicroManager (including probably also your microscope stand). If you switch to MicroManager, it should be relatively straightforward to use one of the modern and very economic diode/solid-state laser base laser engines from e.g. Omicron (or numerous other companies).

Greetings Gabor

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Alan Wainman
Sent: Friday, 14 December 2018 18:57
To: [hidden email]
Subject: Laser engine request

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Hi all,
 
I am a postdoc working with Jordan Raff at the University of Oxford, imaging cell division in Drosophila embryos. We have an old PerkinElmer UltraVIEW Imaging system with a gas laser engine, which we have been struggling to maintain but would like to keep going.
 
We are looking for a PerkinElmer diode laser engine (either MLS1 or MLS2) to add onto the system  - currently using 488, 561 and 440 laser lines. As we hope to keep the system running with Volocity software, it will have to be only a PE laser engine.
 
Would anybody be able to help us out?
 
(If in the UK, we will be happy to dismantle and get the system back to Oxford).

Many thanks in advance for your help.


Alan Wainman

Raff Lab
Sir William Dunn School of Pathology
South Parks Road, Oxford, OX1 3RE
UK
Tel: [phone]

[Gary Laevsky]

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I second what Gabor mentions.

I think the disc on an Ultraview is actually a 21, but that doesn't matter.

All you need to do is use the big 25 pin cable on the front of the unit and
plug that into a serial port on your PC.

All the commands to control the unit are in the 21/22 manual (I can send if
you want).

Then, as Gabor says, you can drive it with anything.  I use Elements, but
uManager would work just as well.  And then use any launch you want.

FYI I am no programmer, and this was easy.

Good Luck!

Best,

Gary

On Mon, Dec 17, 2018 at 11:36 AM Csúcs Gábor <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Alan,
>
> If I am not mistaken, The PerkinElmer Ultraview used  the Yokogawa CSU22
> spinning disk module. This should be possible to control with MicroManager
> (including probably also your microscope stand). If you switch to
> MicroManager, it should be relatively straightforward to use one of the
> modern and very economic diode/solid-state laser base laser engines from
> e.g. Omicron (or numerous other companies).
>
> Greetings                       Gabor
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Alan Wainman
> Sent: Friday, 14 December 2018 18:57
> To: [hidden email]
> Subject: Laser engine request
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi all,
>
> I am a postdoc working with Jordan Raff at the University of Oxford,
> imaging cell division in Drosophila embryos. We have an old PerkinElmer
> UltraVIEW Imaging system with a gas laser engine, which we have been
> struggling to maintain but would like to keep going.
>
> We are looking for a PerkinElmer diode laser engine (either MLS1 or MLS2)
> to add onto the system  - currently using 488, 561 and 440 laser lines. As
> we hope to keep the system running with Volocity software, it will have to
> be only a PE laser engine.
>
> Would anybody be able to help us out?
>
> (If in the UK, we will be happy to dismantle and get the system back to
> Oxford).
>
> Many thanks in advance for your help.
>
>
> Alan Wainman
>
> Raff Lab
> Sir William Dunn School of Pathology
> South Parks Road, Oxford, OX1 3RE
> UK
> Tel: [phone]
>

--
Best,

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Co-Founder, North Atlantic Microscopy Society (NAMS)
https://namsmicroscopy.com/
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310

North Atlantic Microscopy Society Inaugural Symposium at Princeton
University, November 1, 2018

[Pascal Voluer]

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Hi,
Among the numerous other companies, there is Oxxius producing performing, economic and flexible laser engines.
Regards.
Pascal Voluer
[phone]

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Csúcs Gábor
Sent: Tuesday, December 18, 2018 01:28
To: [hidden email]
Subject: Re: Laser engine request

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*****

Dear Alan,

If I am not mistaken, The PerkinElmer Ultraview used  the Yokogawa CSU22 spinning disk module. This should be possible to control with MicroManager (including probably also your microscope stand). If you switch to MicroManager, it should be relatively straightforward to use one of the modern and very economic diode/solid-state laser base laser engines from e.g. Omicron (or numerous other companies).

Greetings Gabor

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Alan Wainman
Sent: Friday, 14 December 2018 18:57
To: [hidden email]
Subject: Laser engine request

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi all,
 
I am a postdoc working with Jordan Raff at the University of Oxford, imaging cell division in Drosophila embryos. We have an old PerkinElmer UltraVIEW Imaging system with a gas laser engine, which we have been struggling to maintain but would like to keep going.
 
We are looking for a PerkinElmer diode laser engine (either MLS1 or MLS2) to add onto the system  - currently using 488, 561 and 440 laser lines. As we hope to keep the system running with Volocity software, it will have to be only a PE laser engine.
 
Would anybody be able to help us out?
 
(If in the UK, we will be happy to dismantle and get the system back to Oxford).

Many thanks in advance for your help.


Alan Wainman

Raff Lab
Sir William Dunn School of Pathology
South Parks Road, Oxford, OX1 3RE
UK
Tel: [phone]

[Seonaid Munro]

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Hello All,

Quorum Technologies, following the acquisition of Volocity software, and with the support of our industry partners, will announce the development of several upgrade options for the Perkin Elmer Ultraview systems early in 2019.  Our efforts include the integration of the latest camera and laser engine technologies with the Ultraview.  We would be happy to discuss potential upgrade paths with existing Ultraview users.  Please contact us at [hidden email].

The pending release of Volocity 6.6, which is scheduled for early next year, will include support for new laser engine technology.

Best regards,
Seonaid Munro
Quorum Technologies Inc.
VP Sales & Marketing

Quorum Technologies Inc.
light at work
Tel: [phone]
Web: www.quorumtechnologies.com

On Dec 17, 2018, at 7:37 PM, Pascal VOLUER <[hidden email]> wrote:

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Hi,
Among the numerous other companies, there is Oxxius producing performing, economic and flexible laser engines.
Regards.
Pascal Voluer
[phone]

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Csúcs Gábor
Sent: Tuesday, December 18, 2018 01:28
To: [hidden email]
Subject: Re: Laser engine request

*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Alan,

If I am not mistaken, The PerkinElmer Ultraview used  the Yokogawa CSU22 spinning disk module. This should be possible to control with MicroManager (including probably also your microscope stand). If you switch to MicroManager, it should be relatively straightforward to use one of the modern and very economic diode/solid-state laser base laser engines from e.g. Omicron (or numerous other companies).

Greetings Gabor

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Alan Wainman
Sent: Friday, 14 December 2018 18:57
To: [hidden email]
Subject: Laser engine request

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi all,

I am a postdoc working with Jordan Raff at the University of Oxford, imaging cell division in Drosophila embryos. We have an old PerkinElmer UltraVIEW Imaging system with a gas laser engine, which we have been struggling to maintain but would like to keep going.

We are looking for a PerkinElmer diode laser engine (either MLS1 or MLS2) to add onto the system  - currently using 488, 561 and 440 laser lines. As we hope to keep the system running with Volocity software, it will have to be only a PE laser engine.

Would anybody be able to help us out?

(If in the UK, we will be happy to dismantle and get the system back to Oxford).

Many thanks in advance for your help.


Alan Wainman

Raff Lab
Sir William Dunn School of Pathology
South Parks Road, Oxford, OX1 3RE
UK
Tel: [phone]

[Ian Dobbie]

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Gary Laevsky <[hidden email]> writes:

> I think the disc on an Ultraview is actually a 21, but that doesn't matter.
>
> All you need to do is use the big 25 pin cable on the front of the unit and
> plug that into a serial port on your PC.
>
> All the commands to control the unit are in the 21/22 manual (I can send if
> you want).
>
> Then, as Gabor says, you can drive it with anything.  I use Elements, but
> uManager would work just as well.  And then use any launch you want.

The problem with this approach is you have to go with long exposure
times to avoid striping in the images due to partial field scans. The
advantage of the PE laser engine (and the prosync box) is it can sync
with the disk and give you full field scans even at short exposure times.

Any other replacement needs major work to get this functionality :-(.

And no Alan you cant have either of our laser engines!

Ian

[Csúcs Gábor-3]

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Dear Ian,

I would like to slightly correct your statement and argue that the suggestion of Gary may still work easily.
Though I don't know the details of the Perkin Elmer system, but typically the synchronization mentioned by you happens between the camera and the spinning disk and not the laser engine. Hence, if the synchronization between the camera and the disk works (is still there) then one should not get these striping artefacts. There is a possibility to trigger the laser illumination directly via the camera and in this case you can optimize also the length of the illumination.
        Even if the synchronization module can't be controlled by the new software, there is a possibility to avoid stripes for short illumination (we practiced this for quite a while with an old CSU-10 unit. If you experiment a little bit with your system, you can easily find also short camera integration times where there are no stripes. E.g. At 50 ms you see stripes, but at 52ms they are gone. By making a simple table you can have a list of "frequently used" illumination time where there are no stripes. Clearly, the synchronisation is the more elegant solution, but there are workarounds (of course you sacrifice some flexibility).
        Of course this whole discussion is obsolete if Quorum technologies will indeed continue to support those UltraView systems.

Greetings Gabor

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ian Dobbie
Sent: Tuesday, 18 December 2018 23:51
To: [hidden email]
Subject: Re: Laser engine request

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Gary Laevsky <[hidden email]> writes:

> I think the disc on an Ultraview is actually a 21, but that doesn't matter.
>
> All you need to do is use the big 25 pin cable on the front of the
> unit and plug that into a serial port on your PC.
>
> All the commands to control the unit are in the 21/22 manual (I can
> send if you want).
>
> Then, as Gabor says, you can drive it with anything.  I use Elements,
> but uManager would work just as well.  And then use any launch you want.

The problem with this approach is you have to go with long exposure times to avoid striping in the images due to partial field scans. The advantage of the PE laser engine (and the prosync box) is it can sync with the disk and give you full field scans even at short exposure times.

Any other replacement needs major work to get this functionality :-(.

And no Alan you cant have either of our laser engines!

Ian