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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** The argon laser on our Nikon A1R died yesterday and I am considering getting a refurbished laser to replace it myself since we are not on a service contract. The actual physical job of removal and replacement of the laser looks pretty simple because the laser box is external and appears to be held in by several screws. I am not sure how complex the alignment would be or if one can expect that the alignment will be set reasonably close by the placement of the laser box. Has anyone attempted this themselves and if so can you suggest if it is a reasonable project to undertake? Thanks- Dave David Knecht, Ph.D. Professor and Head of Microscopy Facility Department of Molecular and Cell Biology U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax) |
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Craig Brideau |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I have done this with the 638 line, and it takes some fiddling but it is possible for mere mortals to do. One key point is to make sure the exit port of the new laser is at the same location as the old one. If you get an identical replacement this should not be an issue. There are pointing optics in the LU that will need to be nudged to get good coupling into the launch fiber. Be very sure to only touch optics the Argon passes through or you will throw off the alignment of the other lines. One final caution is that the Argon passes through an AOM for control. This may need to be recalibrated to properly modulate the lines but you might be OK if you can match the original alignment closely enough. As always, practice appropriate eye safety when working with lasers. Craig On 2013-12-05 9:39 AM, "Knecht, David" <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > The argon laser on our Nikon A1R died yesterday and I am considering > getting a refurbished laser to replace it myself since we are not on a > service contract. The actual physical job of removal and replacement of > the laser looks pretty simple because the laser box is external and appears > to be held in by several screws. I am not sure how complex the alignment > would be or if one can expect that the alignment will be set reasonably > close by the placement of the laser box. Has anyone attempted this > themselves and if so can you suggest if it is a reasonable project to > undertake? Thanks- Dave > > David Knecht, Ph.D. > Professor and Head of Microscopy Facility > Department of Molecular and Cell Biology > U-3125 > 91 N. Eagleville Rd. > University of Connecticut > Storrs, CT 06269 > 860-486-2200 > 860-486-4331 (fax) > |
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Christian Elowsky |
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In reply to this post by Knecht, David
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I would suggest looking at a solid state 488 laser instead. -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Knecht, David Sent: Thursday, December 05, 2013 8:08 AM To: [hidden email] Subject: Laser replacement on Nikon A1R ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** The argon laser on our Nikon A1R died yesterday and I am considering getting a refurbished laser to replace it myself since we are not on a service contract. The actual physical job of removal and replacement of the laser looks pretty simple because the laser box is external and appears to be held in by several screws. I am not sure how complex the alignment would be or if one can expect that the alignment will be set reasonably close by the placement of the laser box. Has anyone attempted this themselves and if so can you suggest if it is a reasonable project to undertake? Thanks- Dave David Knecht, Ph.D. Professor and Head of Microscopy Facility Department of Molecular and Cell Biology U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax) |
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Hugh Newman |
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In reply to this post by Knecht, David
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Unless you are doing CFP/YFP FRET multi line argon lasers are not really recommended due to cost, noise, heat, and laser life. If you are using just one line like 488, go for a solid state. I would talk to the AIS or SBAM in your region from Nikon to discuss alignment. Hugh Newman > Date: Thu, 5 Dec 2013 14:08:13 +0000 > From: [hidden email] > Subject: Laser replacement on Nikon A1R > To: [hidden email] > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > The argon laser on our Nikon A1R died yesterday and I am considering getting a refurbished laser to replace it myself since we are not on a service contract. The actual physical job of removal and replacement of the laser looks pretty simple because the laser box is external and appears to be held in by several screws. I am not sure how complex the alignment would be or if one can expect that the alignment will be set reasonably close by the placement of the laser box. Has anyone attempted this themselves and if so can you suggest if it is a reasonable project to undertake? Thanks- Dave > > David Knecht, Ph.D. > Professor and Head of Microscopy Facility > Department of Molecular and Cell Biology > U-3125 > 91 N. Eagleville Rd. > University of Connecticut > Storrs, CT 06269 > 860-486-2200 > 860-486-4331 (fax) |
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