Lasers for ablation of biological samples

[DarrenRobinson]

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Hi,

We currently use a nitrogen laser ablation system (Micropoint from Andor) to perform wounding studies on drosophila embryos.  The laser is capable of reliably inducing a local injury to superficial tissue that is visible when the laser is fired.  Does anyone have any experience using other lasers for this kind of work?  Would a high power 405nm laser work for example, or does ablation require using the lower wavelength pulsed light that is produced by lasers such as the one we currently use?

Many thanks,

Darren.

[Straatman, Kees (Dr.)]

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Hi Darren,

I have had users using the 405 nm laser on a CLSM for ablation; see for example https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331284/

Best wishes

Kees

Dr Ir K.R. Straatman FRMS
Advanced Imaging Facility
University of Leicester
www.le.ac.uk/advanced-imaging-facility




-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Darren Robinson
Sent: 06 May 2021 08:52
To: [hidden email]
Subject: Lasers for ablation of biological samples

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Hi,

We currently use a nitrogen laser ablation system (Micropoint from Andor) to perform wounding studies on drosophila embryos.  The laser is capable of reliably inducing a local injury to superficial tissue that is visible when the laser is fired.  Does anyone have any experience using other lasers for this kind of work?  Would a high power 405nm laser work for example, or does ablation require using the lower wavelength pulsed light that is produced by lasers such as the one we currently use?

Many thanks,

Darren.

[Advanced Imaging Lab]

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 Hi Darren,

We have used our Zeiss PALM-Laser capture microdissection microscope to cut
motor neurons of zebrafish embryos.

Best regards,
Esther

*Dr. Esther G.L. Koh* :: Head, Advanced Imaging Laboratory :: Life Sciences
Institute Immunology Programme :: National University of Singapore :: Centre
for Life Sciences, 28 Medical Drive #03-06E, Singapore 117456

On Thu, 6 May 2021 at 20:37, Straatman, Kees (Dr.) <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Darren,
>
> I have had users using the 405 nm laser on a CLSM for ablation; see for
> example https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331284/
>
> Best wishes
>
> Kees
>
> Dr Ir K.R. Straatman FRMS
> Advanced Imaging Facility
> University of Leicester
> www.le.ac.uk/advanced-imaging-facility
>
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List <[hidden email]> On
> Behalf Of Darren Robinson
> Sent: 06 May 2021 08:52
> To: [hidden email]
> Subject: Lasers for ablation of biological samples
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
> https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&amp;data=04%7C01%7Ckrs5%40LEICESTER.AC.UK%7C415ae09fd505489737c008d910654943%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C1%7C637558849474843047%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&amp;sdata=jTwe0dehsD8m0DI3ue154MNVjkAAOVd%2Bv7RPZYb6QDs%3D&amp;reserved=0
> Post images on
> https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com%2F&amp;data=04%7C01%7Ckrs5%40LEICESTER.AC.UK%7C415ae09fd505489737c008d910654943%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C1%7C637558849474843047%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&amp;sdata=w96li2%2FqGC0xxSuOhE1CgQne6NNzbbHbqH9aHIeOsoA%3D&amp;reserved=0
> and include the link in your posting.
> *****
>
> Hi,
>
> We currently use a nitrogen laser ablation system (Micropoint from Andor)
> to perform wounding studies on drosophila embryos.  The laser is capable of
> reliably inducing a local injury to superficial tissue that is visible when
> the laser is fired.  Does anyone have any experience using other lasers for
> this kind of work?  Would a high power 405nm laser work for example, or
> does ablation require using the lower wavelength pulsed light that is
> produced by lasers such as the one we currently use?
>
> Many thanks,
>
> Darren.
>

--
Dr. Esther G.L. Koh :: Head, Advanced Imaging Laboratory :: Life Sciences
Institute Immunology Programme :: National University of
Singapore :: Centre for Life Sciences, 28 Medical Drive #03-06E, Singapore
117456

[samuel connell]

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Hi Darren,

High-powered pulsed lasers are typically understood to be the gold standard
in the ablation community. There are other approaches used that are
functional, but my understanding is that a continuous wave (CW) 405 laser
would typically need a fluorophore to act as a sensitizer. A familiar
example is using Hoechst or BrdU as a sensitizer for DNA damage
experiments when irradiated with 405. More specifically, one can use a
pulsed UV laser to directly induce DNA damage (without a sensitizer).
Similarly, pulsed high-powered lasers can be used to directly cause a range
of damage from cellular ablation and blood clots, to diffraction-limited
damage.

3i has a fair amount of experience in this domain, having been a reseller
for the Micropoint systems for many years as well as working a decade ago
to develop a 532nm (and subsequently 355nm) pulsed laser system for
ablation.

Cheers,
-
Sam

https://www.intelligent-imaging.com/photomanipulation



On Thu, May 6, 2021 at 1:02 AM Darren Robinson <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi,
>
> We currently use a nitrogen laser ablation system (Micropoint from Andor)
> to perform wounding studies on drosophila embryos.  The laser is capable of
> reliably inducing a local injury to superficial tissue that is visible when
> the laser is fired.  Does anyone have any experience using other lasers for
> this kind of work?  Would a high power 405nm laser work for example, or
> does ablation require using the lower wavelength pulsed light that is
> produced by lasers such as the one we currently use?
>
> Many thanks,
>
> Darren.
>