Leica HyD detectors?

[Adrian Smith-6]

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Hi all,

We are about to have two HyD detectors installed on our SP5.

I'm just wondering about which position to put them in on the 5 detector array? ie what makes the most sense for use with or without conventional PMTs? (we will have 3 conventional PMTs as well).

I'm guessing Leica has some recommendations but I'm curious as to what others have done.

Regards,

Adrian Smith
Centenary Institute, Sydney, Australia

[Renato A. Mortara]

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Just to mention that we have got our SP5 with two HyD and two PMTs  
since last June. There were minor software hickups on the way but all  
efficiently solved by our local Leica support.

Hope you get your array of 5 working well.

Renato

Renato A. Mortara
Disciplina de Parasitologia
UNIFESP Escola Paulista de Medicina
R. Botucatu, 862 6o andar
04023-062
S?o Paulo SP
Brasil


Citando Adrian Smith <[hidden email]>:

>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi all,
>
> We are about to have two HyD detectors installed on our SP5.
>
> I'm just wondering about which position to put them in on the 5  
> detector array? ie what makes the most sense for use with or without  
>  conventional PMTs? (we will have 3 conventional PMTs as well).
>
> I'm guessing Leica has some recommendations but I'm curious as to  
> what others have done.
>
> Regards,
>
> Adrian Smith
> Centenary Institute, Sydney, Australia
>
>

[Alessandro Esposito]

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Dear Adrian,
    we have an SP5 that originally was equipped with 3 PMTs and then
upgraded with 2 HyDs. Leica suggested to install them in position 2 and 4
and I agreed with this. Apparently, position 3 is not available for the HyDs
because they are too bulky. Position 2 and 4 are the ones after the first
reflection from the mirror barriers after PTM3. I hope to remember this
correctly! ;P

Cheers,

Alessandro

[Olivier Bardot]

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Hello
It depends if you have bought two HyD AND the update from 3 to 5 detectors.
If not, they will remove 2 PMT, replace first and last by HyD and keep central PMT
(referred to wavelength),as far as I know.
Two is the minimum choice.
We have just one and it has to be last detector. It is great for all which use
sequential scan. But I do mainly live experiments with GFP fusion proteins and
FM4.64 as control. If I want both for rapid scan, I have to acquire GFP on PMT
and FM4.64 on HyD. I loose all benefits of HyD detectors.
Hope this helps

[yuansheng sun]

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Dear Adrian,

On our Leica SP5 X, we had 5 conventional PMTs for fluorescence imaging.
We just swapped two for HyDs.  Due to their bulky size and recommendations
from Leica, we put our HyDs into the second and four positions.  I think
this configuration is reasonable and gives us a lot of freedom to use HyDs
as well as regular PMTs.

Sheng

Yuansheng Sun, PH.D.
Keck Center for Cellular Imaging
University of Virginia




On Mon, Mar 19, 2012 at 5:09 AM, Olivier Bardot <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello
> It depends if you have bought two HyD AND the update from 3 to 5 detectors.
> If not, they will remove 2 PMT, replace first and last by HyD and keep
> central PMT
> (referred to wavelength),as far as I know.
> Two is the minimum choice.
> We have just one and it has to be last detector. It is great for all which
> use
> sequential scan. But I do mainly live experiments with GFP fusion proteins
> and
> FM4.64 as control. If I want both for rapid scan, I have to acquire GFP on
> PMT
> and FM4.64 on HyD. I loose all benefits of HyD detectors.
> Hope this helps
>