Leica SP5 Question

[Ellen T. Arena]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

I am hoping someone can clarify something for me regarding a 'settings'
option on the Leica SP5.  Within 'Configurations -> Settings' - there is an
option to check Line Average - called 'Line Average during Live Acquisition'
- what does this mean exactly?  And how is it different from the Line
Averages you can set within 'Acquire -> Acquisition'??

Many thanks!!!

Ellen

[Pascal Weber]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

In fact it is the same if you do a snapshot. But it is better  if are not use the
average during live mode. You expose less time your sample.

[Romin, Yevgeniy/Sloan Kettering Institute]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi Ellen

This means that you will see the averaging when you scan using the "live" mode, not just when you hit the "start" button to acquire the image.  This is usually used in resonant scanning mode, since there averaging is sometimes needed to make out details.  This will naturally slow down the "live" scan speed.

Yevgeniy
________________________________________
From: Confocal Microscopy List [[hidden email]] On Behalf Of Ellen T. Arena [[hidden email]]
Sent: Friday, March 15, 2013 6:29 AM
To: [hidden email]
Subject: Leica SP5 Question

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

I am hoping someone can clarify something for me regarding a 'settings'
option on the Leica SP5.  Within 'Configurations -> Settings' - there is an
option to check Line Average - called 'Line Average during Live Acquisition'
- what does this mean exactly?  And how is it different from the Line
Averages you can set within 'Acquire -> Acquisition'??

Many thanks!!!

Ellen


 
     =====================================================================
     
     Please note that this e-mail and any files transmitted from
     Memorial Sloan-Kettering Cancer Center may be privileged, confidential,
     and protected from disclosure under applicable law. If the reader of
     this message is not the intended recipient, or an employee or agent
     responsible for delivering this message to the intended recipient,
     you are hereby notified that any reading, dissemination, distribution,
     copying, or other use of this communication or any of its attachments
     is strictly prohibited.  If you have received this communication in
     error, please notify the sender immediately by replying to this message
     and deleting this message, any attachments, and all copies and backups
     from your computer.

[yuansheng sun]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Ellen,

If the line average option in the configuration is not checked, the line
average will not be applied in the live mode even when you put a line
average number (say 2) in the acquisition page; it is only applied when you
hit capture or start button.  So checking the line average option in the
configuration setting page will just enforce live average in the live mode.
 By the default, you do not want to do the line average in the live mode,
which mainly allows you get a feel about the field of view so that you can
quickly adjust focus and determine your imaging settings, such as laser
power, etc.  You do not waste your time in the live mode, especially for
live specimens.  So most likely, you do not need line average in the live
mode.  For the final data acquisition by capture or start, you may need
line average to achieve a better SNR.  I hope this helps.

- sheng,  another user of sp5


On Friday, March 15, 2013, Ellen T. Arena wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> I am hoping someone can clarify something for me regarding a 'settings'
> option on the Leica SP5.  Within 'Configurations -> Settings' - there is an
> option to check Line Average - called 'Line Average during Live
> Acquisition'
> - what does this mean exactly?  And how is it different from the Line
> Averages you can set within 'Acquire -> Acquisition'??
>
> Many thanks!!!
>
> Ellen
>