Leica SP5 - adding delays between different positions in a multi point timelapse

[Alessandro Esposito]

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Dear all,
    in order to synchronise the acquisition with external detectors, I would
need to add a delay after each position in a multi point time series using a
Leica SP5 confocal microscope.

I would like to create a list of positions with the mark and find tool. Then
setup a time lapse with the followins sequence:

1. move to next position (position 1 if start of loop)
2. acquire image
3. wait N seconds
4. loop to 1 until all position scanned
5. wait T minutes
6. loop to 1 until all time frames acquired

We have the last version of LAS AF compatible with the SP5. Do you know if
this is possible?

Cheers,

Alessandro

[Ekaterina PAPUSHEVA-2]

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Hi Alessandro,
I only managed to such stuff with live data mode-additional software that Leica sells.
But, i am not sure timing will be precise enough for you.... Better try it with a demo dongle

Good luck!
Katja

Sent from my HTC

----- Reply message -----
From: "Alessandro Esposito" <[hidden email]>
To: "[hidden email]" <[hidden email]>
Subject: Leica SP5 - adding delays between different positions in a multi point timelapse
Date: Wed, Aug 14, 2013 5:58 pm



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Dear all,
    in order to synchronise the acquisition with external detectors, I would
need to add a delay after each position in a multi point time series using a
Leica SP5 confocal microscope.

I would like to create a list of positions with the mark and find tool. Then
setup a time lapse with the followins sequence:

1. move to next position (position 1 if start of loop)
2. acquire image
3. wait N seconds
4. loop to 1 until all position scanned
5. wait T minutes
6. loop to 1 until all time frames acquired

We have the last version of LAS AF compatible with the SP5. Do you know if
this is possible?

Cheers,

Alessandro

[Alessandro Esposito]

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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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Hi Katja,
   perhaps I gave up too early on the Live Data Mode. The one time I tried, I
was not able to combine the find/mark option with the delay. Precision would
not be an issue, I can add seconds of delay even if imaging would be slower
for this application I do not mind.

I'll try tomorrow but if you have a suggestion on how to configure it would
be great. I rarely use the Live Data Mode and the "help" button is not that
useful.

Thanks,

Alessandro

[Ekaterina PAPUSHEVA-2]

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Hi Alessandro,

I am on holiday without an access to leica sp5 until 2.9.
You have to set positions individually and program a delay between them, i think... But i do not remember exactly, there was some time since we did it.
If you will still need it on 2.9, i can have a look and let you know!

Good luck,
Katja

Sent from my HTC

----- Reply message -----
From: "Alessandro Esposito" <[hidden email]>
To: "[hidden email]" <[hidden email]>
Subject: Leica SP5 - adding delays between different positions in a multi point timelapse
Date: Wed, Aug 14, 2013 6:59 pm



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Hi Katja,
   perhaps I gave up too early on the Live Data Mode. The one time I tried, I
was not able to combine the find/mark option with the delay. Precision would
not be an issue, I can add seconds of delay even if imaging would be slower
for this application I do not mind.

I'll try tomorrow but if you have a suggestion on how to configure it would
be great. I rarely use the Live Data Mode and the "help" button is not that
useful.

Thanks,

Alessandro