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Light reading on optical nanoscopy

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Peng Xi-2

Light reading on optical nanoscopy

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Dear List,
I am blogging on optical nanoscopy, in a very casual mode.
http://xipeng.wordpress.com/2012/08/16/how-optical-super-resolution-is-achieved-1/
It is originally written in Chinese, after I gave a related plenary
talk in May 2012. Last talk, in the noon time. And the audiences were from
all sorts of disciplines, from fresh graduate students to renowned
professors. Therefore, I decided to make the talk interesting, and easy to
follow by everyone. It turns out to be very successful -- much better than
equations and diagrams. So, I decide to broadcast it by blogging. :)
Hope you like it.

Sincerely,
Peng Xi
Ph. D. Associate Professor
Dept. of Biomedical Engineering, College of Engineering
Peking University, Beijing, China
Tel: +86 10-6276 7155
Email: [hidden email]

Mark Cannell-2

Re: Light reading on optical nanoscopy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hmm, A very myopic blog on a subject with a rich past. It was appreciated that the Abbe 'limit' was not a limit well before Stephan Hell's work. Suggest you might like to research the subject matter a bit deeper?

Cheers

PS I hope others don't start advertising their 'blogs' on this list, we ban commercial 'blogs', perhaps this should be extend?

On 23/08/2012, at 1:32 AM, Peng Xi <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear List,
> I am blogging on optical nanoscopy, in a very casual mode.
> http://xipeng.wordpress.com/2012/08/16/how-optical-super-resolution-is-achieved-1/
> It is originally written in Chinese, after I gave a related plenary
> talk in May 2012. Last talk, in the noon time. And the audiences were from
> all sorts of disciplines, from fresh graduate students to renowned
> professors. Therefore, I decided to make the talk interesting, and easy to
> follow by everyone. It turns out to be very successful -- much better than
> equations and diagrams. So, I decide to broadcast it by blogging. :)
> Hope you like it.
>
> Sincerely,
> Peng Xi
> Ph. D. Associate Professor
> Dept. of Biomedical Engineering, College of Engineering
> Peking University, Beijing, China
> Tel: +86 10-6276 7155
> Email: [hidden email]

Mark B. Cannell Ph.D. FRSNZ
Professor of Cardiac Cell Biology
School of Physiology& Pharmacology
Medical Sciences Building
University of Bristol
Bristol
BS8 1TD UK

[hidden email]

Peng Xi-2

Re: Light reading on optical nanoscopy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear Mark,
I am not sure what you mean by "well before". Super-resolution is a
term that usually refers to techniques that provides theoretically
'infinitely small' resolution, or down to single molecule size. That's why
although confocal is already better (1.4x better) in resolution, it is
generally not treated as super-resolution. And so to multiphoton microscopy.
If you have a better candidate on inventing super-resolution, please let
me and everybody know. I am sure that people are keen to know this.

Sincerely,
Peng Xi
Ph. D. Associate Professor
Dept. of Biomedical Engineering, College of Engineering
Peking University, Beijing, China
Tel: +86 10-6276 7155
Email: [hidden email]

On Thu, Aug 23, 2012 at 11:51 AM, Mark Cannell
<[hidden email]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hmm, A very myopic blog on a subject with a rich past. It was appreciated
> that the Abbe 'limit' was not a limit well before Stephan Hell's work.
> Suggest you might like to research the subject matter a bit deeper?
>
> Cheers
>
> PS I hope others don't start advertising their 'blogs' on this list, we
> ban commercial 'blogs', perhaps this should be extend?
>
>
> On 23/08/2012, at 1:32 AM, Peng Xi <[hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Dear List,
> > I am blogging on optical nanoscopy, in a very casual mode.
> >
> http://xipeng.wordpress.com/2012/08/16/how-optical-super-resolution-is-achieved-1/
> > It is originally written in Chinese, after I gave a related plenary
> > talk in May 2012. Last talk, in the noon time. And the audiences were
> from
> > all sorts of disciplines, from fresh graduate students to renowned
> > professors. Therefore, I decided to make the talk interesting, and easy
> to
> > follow by everyone. It turns out to be very successful -- much better
> than
> > equations and diagrams. So, I decide to broadcast it by blogging. :)
> > Hope you like it.
> >
> > Sincerely,
> > Peng Xi
> > Ph. D. Associate Professor
> > Dept. of Biomedical Engineering, College of Engineering
> > Peking University, Beijing, China
> > Tel: +86 10-6276 7155
> > Email: [hidden email]
>
> Mark B. Cannell Ph.D. FRSNZ
> Professor of Cardiac Cell Biology
> School of Physiology& Pharmacology
> Medical Sciences Building
> University of Bristol
> Bristol
> BS8 1TD UK
>
> [hidden email]
>

Mark Cannell-2

Re: Light reading on optical nanoscopy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Just a couple of examples, they are probably even earlier papers … the idea of a resolution 'limit' for self luminous objects was dismissed in the 1950's -if not earlier. Here's a few papers that may lead to more:

Exchanging time for spatial information to increase resolution:

LUKOSZ W. Optical systems with resolving powers exceeding the classical limit. II. JOSA. Optical Society of America; 1967;57(7):932–9.

General discussion about super resolution

McCutchen CW. Superresolution in microscopy and the Abbe resolution limit. JOSA. Optical Society of America; 1967;57(10):1190–0.

Using a grating to increase resolution;

BACHL A, LUKOSZ W. Experiments on Superresolution Imaging of a Reduced Object Field. J Opt Soc Am. 1967;57(2):163–&.

Resolution enhancement by non-linear optical effects:

Ehrlich DJ, Tsao JY. Nonreciprocal laser-microchemical processing: Spatial resolution limits and demonstration of 0.2-μm linewidths. Appl. Phys. Lett. 1984;44(2):267.

Cheers

On 23/08/2012, at 7:59 AM, Peng Xi <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear Mark,
> I am not sure what you mean by "well before". Super-resolution is a
> term that usually refers to techniques that provides theoretically
> 'infinitely small' resolution, or down to single molecule size. That's why
> although confocal is already better (1.4x better) in resolution, it is
> generally not treated as super-resolution. And so to multiphoton microscopy.
> If you have a better candidate on inventing super-resolution, please let
> me and everybody know. I am sure that people are keen to know this.
>
>
> Sincerely,
> Peng Xi
> Ph. D. Associate Professor
> Dept. of Biomedical Engineering, College of Engineering
> Peking University, Beijing, China
> Tel: +86 10-6276 7155
> Email: [hidden email]
>
>
> On Thu, Aug 23, 2012 at 11:51 AM, Mark Cannell
> <[hidden email]>wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Hmm, A very myopic blog on a subject with a rich past. It was appreciated
>> that the Abbe 'limit' was not a limit well before Stephan Hell's work.
>> Suggest you might like to research the subject matter a bit deeper?
>>
>> Cheers
>>
>> PS I hope others don't start advertising their 'blogs' on this list, we
>> ban commercial 'blogs', perhaps this should be extend?
>>
>>
>> On 23/08/2012, at 1:32 AM, Peng Xi <[hidden email]> wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Dear List,
>>> I am blogging on optical nanoscopy, in a very casual mode.
>>>
>> http://xipeng.wordpress.com/2012/08/16/how-optical-super-resolution-is-achieved-1/
>>> It is originally written in Chinese, after I gave a related plenary
>>> talk in May 2012. Last talk, in the noon time. And the audiences were
>> from
>>> all sorts of disciplines, from fresh graduate students to renowned
>>> professors. Therefore, I decided to make the talk interesting, and easy
>> to
>>> follow by everyone. It turns out to be very successful -- much better
>> than
>>> equations and diagrams. So, I decide to broadcast it by blogging. :)
>>> Hope you like it.
>>>
>>> Sincerely,
>>> Peng Xi
>>> Ph. D. Associate Professor
>>> Dept. of Biomedical Engineering, College of Engineering
>>> Peking University, Beijing, China
>>> Tel: +86 10-6276 7155
>>> Email: [hidden email]
>>
>> Mark B. Cannell Ph.D. FRSNZ
>> Professor of Cardiac Cell Biology
>> School of Physiology& Pharmacology
>> Medical Sciences Building
>> University of Bristol
>> Bristol
>> BS8 1TD UK
>>
>> [hidden email]
>>

Mark B. Cannell Ph.D. FRSNZ
Professor of Cardiac Cell Biology
School of Physiology& Pharmacology
Medical Sciences Building
University of Bristol
Bristol
BS8 1TD UK

[hidden email]

Pascal Weber

Re: Light reading on optical nanoscopy

In reply to this post by Peng Xi-2

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

I agree with Mr. Xi. super resolution images that are calculated. Is this real? I
actually came to the same resolution between a confocal microscope and a
two photon. And in both cases I can make images of the living and study
interaction on live models.

Alberto Diaspro

Re: Light reading on optical nanoscopy

In reply to this post by Mark Cannell-2

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

I love The Lukosz work, i think that One Could also considering works by Toraldo di Francia on super resolution starting from this fundamental reading on The resolution definition:
Toraldo di Francia (1955) Resolving power and information. JOSA 47(7), 497-501.
Ciao
Alby

Mark on Your agenda www.maf13.org

Inviato da iPad

Il giorno 23/ago/2012, alle ore 09:30, Mark Cannell <[hidden email]> ha scritto:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Just a couple of examples, they are probably even earlier papers … the idea of a resolution 'limit' for self luminous objects was dismissed in the 1950's -if not earlier. Here's a few papers that may lead to more:
>
> Exchanging time for spatial information to increase resolution:
>
> LUKOSZ W. Optical systems with resolving powers exceeding the classical limit. II. JOSA. Optical Society of America; 1967;57(7):932–9.
>
> General discussion about super resolution
>
> McCutchen CW. Superresolution in microscopy and the Abbe resolution limit. JOSA. Optical Society of America; 1967;57(10):1190–0.
>
>
> Using a grating to increase resolution;
>
> BACHL A, LUKOSZ W. Experiments on Superresolution Imaging of a Reduced Object Field. J Opt Soc Am. 1967;57(2):163–&.
>
> Resolution enhancement by non-linear optical effects:
>
> Ehrlich DJ, Tsao JY. Nonreciprocal laser-microchemical processing: Spatial resolution limits and demonstration of 0.2-μm linewidths. Appl. Phys. Lett. 1984;44(2):267.
>
> Cheers
>
>
> On 23/08/2012, at 7:59 AM, Peng Xi <[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Dear Mark,
>> I am not sure what you mean by "well before". Super-resolution is a
>> term that usually refers to techniques that provides theoretically
>> 'infinitely small' resolution, or down to single molecule size. That's why
>> although confocal is already better (1.4x better) in resolution, it is
>> generally not treated as super-resolution. And so to multiphoton microscopy.
>> If you have a better candidate on inventing super-resolution, please let
>> me and everybody know. I am sure that people are keen to know this.
>>
>>
>> Sincerely,
>> Peng Xi
>> Ph. D. Associate Professor
>> Dept. of Biomedical Engineering, College of Engineering
>> Peking University, Beijing, China
>> Tel: +86 10-6276 7155
>> Email: [hidden email]
>>
>>
>> On Thu, Aug 23, 2012 at 11:51 AM, Mark Cannell
>> <[hidden email]>wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Hmm, A very myopic blog on a subject with a rich past. It was appreciated
>>> that the Abbe 'limit' was not a limit well before Stephan Hell's work.
>>> Suggest you might like to research the subject matter a bit deeper?
>>>
>>> Cheers
>>>
>>> PS I hope others don't start advertising their 'blogs' on this list, we
>>> ban commercial 'blogs', perhaps this should be extend?
>>>
>>>
>>> On 23/08/2012, at 1:32 AM, Peng Xi <[hidden email]> wrote:
>>>
>>>> *****
>>>> To join, leave or search the confocal microscopy listserv, go to:
>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>>> *****
>>>>
>>>> Dear List,
>>>> I am blogging on optical nanoscopy, in a very casual mode.
>>>>
>>> http://xipeng.wordpress.com/2012/08/16/how-optical-super-resolution-is-achieved-1/
>>>> It is originally written in Chinese, after I gave a related plenary
>>>> talk in May 2012. Last talk, in the noon time. And the audiences were
>>> from
>>>> all sorts of disciplines, from fresh graduate students to renowned
>>>> professors. Therefore, I decided to make the talk interesting, and easy
>>> to
>>>> follow by everyone. It turns out to be very successful -- much better
>>> than
>>>> equations and diagrams. So, I decide to broadcast it by blogging. :)
>>>> Hope you like it.
>>>>
>>>> Sincerely,
>>>> Peng Xi
>>>> Ph. D. Associate Professor
>>>> Dept. of Biomedical Engineering, College of Engineering
>>>> Peking University, Beijing, China
>>>> Tel: +86 10-6276 7155
>>>> Email: [hidden email]
>>>
>>> Mark B. Cannell Ph.D. FRSNZ
>>> Professor of Cardiac Cell Biology
>>> School of Physiology& Pharmacology
>>> Medical Sciences Building
>>> University of Bristol
>>> Bristol
>>> BS8 1TD UK
>>>
>>> [hidden email]
>>>
>
> Mark B. Cannell Ph.D. FRSNZ
> Professor of Cardiac Cell Biology
> School of Physiology& Pharmacology
> Medical Sciences Building
> University of Bristol
> Bristol
> BS8 1TD UK
>
> [hidden email]

Guy Cox-2

Re: Light reading on optical nanoscopy

In reply to this post by Peng Xi-2

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Apart from Mark's suggestions, Synge proposed near-field microscopy in 1928 (Philosophical Magazine 6, 356). It was first demonstrated in visible light in 1984 (D.W. Pohl, W. Denk, and M. Lanz (1984). "Optical stethoscopy: Image recording with resolution λ/20". Appl. Phys. Lett. 44 (7): 651.) though it had been achieved in the microwave region as early as 1972. This is all way before the concept of STED had even been proposed (1994) let alone demonstrated (2002).

It's not fair to say that only 'unlimited' methods are true super-resolution. Both 4-pi (proposed by Cremer & Cremer in 1971) and linear structured illumination are generally regarded (and marketed) as super-resolution. Linear structured illumination was demonstrated two years before STED (Gustafsson, M.G.L. 2000 Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. Journal of Microscopy 198 (2) , pp. 82-87). I5 microscopy, another technique offering substantial but not unlimited super-resolution, was achieved even earlier (Gustafsson, Agard & Sedat, Journal of Microscopy, 195, 10-16, 1999).

Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox   2nd edition, 2012 CRC Press
     http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Aust. Centre for Microscopy & Microanalysis, F09,
University of Sydney, NSW 2006
______________________________________________
Phone +61 2 9351 3176     Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
      http://www.guycox.net

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Peng Xi
Sent: Thursday, 23 August 2012 4:59 PM
To: [hidden email]
Subject: Re: Light reading on optical nanoscopy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear Mark,
I am not sure what you mean by "well before". Super-resolution is a
term that usually refers to techniques that provides theoretically
'infinitely small' resolution, or down to single molecule size. That's why
although confocal is already better (1.4x better) in resolution, it is
generally not treated as super-resolution. And so to multiphoton microscopy.
If you have a better candidate on inventing super-resolution, please let
me and everybody know. I am sure that people are keen to know this.

Sincerely,
Peng Xi
Ph. D. Associate Professor
Dept. of Biomedical Engineering, College of Engineering
Peking University, Beijing, China
Tel: +86 10-6276 7155
Email: [hidden email]

On Thu, Aug 23, 2012 at 11:51 AM, Mark Cannell
<[hidden email]>wrote:

Peng Xi-2

Re: Light reading on optical nanoscopy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear Guy,
I fully agree with you that in a broader definition, light sheet
microscopy, 4Pi and SIM should be included. Actually I have done so in a
review article of mine:

Y. Ding, *P. Xi**, Q. Ren, “Hacking the optical diffraction limit: Review
on recent developments of fluorescence
nanoscopy<http://bme.pku.edu.cn/~xipeng/resume/Hacking%20the%20optical%20diffraction%20limit.pdf>”,
Chinese Science Bulletin, 56(18) 1857-1876 (2011) Open access
http://www.springerlink.com/content/j528591251261mn1/

Yet, in this blog, I wish to keep it this way to follow one major
theme. This theme is also in coherent with Stefan Hell's Science 2009
review "Far-field optical nanoscopy" and Xiaowei Zhuang's Cell 2010 review.
So I will stay with this restricted definination of super-resolution, which
can be found from Stefan Hell 2009 Nature Methods "Microscopy and its focal
switch" 6(1) 24-32.
Also to keep this blog still "light" without carrying so much historic
burden, I will keep it this way, but to add the relevant references at the
end of the blog page.

Peng
On Thu, Aug 23, 2012 at 4:17 PM, Guy Cox <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Apart from Mark's suggestions, Synge proposed near-field microscopy in
> 1928 (Philosophical Magazine 6, 356). It was first demonstrated in visible
> light in 1984 (D.W. Pohl, W. Denk, and M. Lanz (1984). "Optical
> stethoscopy: Image recording with resolution λ/20". Appl. Phys. Lett. 44
> (7): 651.) though it had been achieved in the microwave region as early as
> 1972. This is all way before the concept of STED had even been proposed
> (1994) let alone demonstrated (2002).
>
> It's not fair to say that only 'unlimited' methods are true
> super-resolution. Both 4-pi (proposed by Cremer & Cremer in 1971) and
> linear structured illumination are generally regarded (and marketed) as
> super-resolution. Linear structured illumination was demonstrated two
> years before STED (Gustafsson, M.G.L. 2000 Surpassing the lateral
> resolution limit by a factor of two using structured illumination
> microscopy. Journal of Microscopy 198 (2) , pp. 82-87). I5 microscopy,
> another technique offering substantial but not unlimited super-resolution,
> was achieved even earlier (Gustafsson, Agard & Sedat, Journal of
> Microscopy, 195, 10-16, 1999).
>
> Guy
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox 2nd edition, 2012 CRC Press
> http://www.guycox.com/optical.htm
> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Aust. Centre for Microscopy & Microanalysis, F09,
> University of Sydney, NSW 2006
> ______________________________________________
> Phone +61 2 9351 3176 Fax +61 2 9351 7682
> Mobile 0413 281 861
> ______________________________________________
> http://www.guycox.net
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Peng Xi
> Sent: Thursday, 23 August 2012 4:59 PM
> To: [hidden email]
> Subject: Re: Light reading on optical nanoscopy
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear Mark,
> I am not sure what you mean by "well before". Super-resolution is a
> term that usually refers to techniques that provides theoretically
> 'infinitely small' resolution, or down to single molecule size. That's why
> although confocal is already better (1.4x better) in resolution, it is
> generally not treated as super-resolution. And so to multiphoton
> microscopy.
> If you have a better candidate on inventing super-resolution, please let
> me and everybody know. I am sure that people are keen to know this.
>
>
> Sincerely,
> Peng Xi
> Ph. D. Associate Professor
> Dept. of Biomedical Engineering, College of Engineering
> Peking University, Beijing, China
> Tel: +86 10-6276 7155
> Email: [hidden email]
>
>
> On Thu, Aug 23, 2012 at 11:51 AM, Mark Cannell
> <[hidden email]>wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Hmm, A very myopic blog on a subject with a rich past. It was
> appreciated
> > that the Abbe 'limit' was not a limit well before Stephan Hell's work.
> > Suggest you might like to research the subject matter a bit deeper?
> >
> > Cheers
> >
> > PS I hope others don't start advertising their 'blogs' on this list, we
> > ban commercial 'blogs', perhaps this should be extend?
> >
> >
> > On 23/08/2012, at 1:32 AM, Peng Xi <[hidden email]> wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > *****
> > >
> > > Dear List,
> > > I am blogging on optical nanoscopy, in a very casual mode.
> > >
> >
> http://xipeng.wordpress.com/2012/08/16/how-optical-super-resolution-is-achieved-1/
> > > It is originally written in Chinese, after I gave a related plenary
> > > talk in May 2012. Last talk, in the noon time. And the audiences were
> > from
> > > all sorts of disciplines, from fresh graduate students to renowned
> > > professors. Therefore, I decided to make the talk interesting, and easy
> > to
> > > follow by everyone. It turns out to be very successful -- much better
> > than
> > > equations and diagrams. So, I decide to broadcast it by blogging. :)
> > > Hope you like it.
> > >
> > > Sincerely,
> > > Peng Xi
> > > Ph. D. Associate Professor
> > > Dept. of Biomedical Engineering, College of Engineering
> > > Peking University, Beijing, China
> > > Tel: +86 10-6276 7155
> > > Email: [hidden email]
> >
> > Mark B. Cannell Ph.D. FRSNZ
> > Professor of Cardiac Cell Biology
> > School of Physiology& Pharmacology
> > Medical Sciences Building
> > University of Bristol
> > Bristol
> > BS8 1TD UK
> >
> > [hidden email]
> >
>

--
席鹏
特聘研究员
北京大学工学院生物医学工程系
地址：中关村北大街北京大学医院A536室
邮编：100084
电话：010-6276 7155
Email: [hidden email]
http://dx.plos.org/10.1371/journal.pone.0040003

Sincerely,
Peng Xi
Ph. D. Associate Professor
Dept. of Biomedical Engineering, College of Engineering
Peking University, Beijing, China
Tel: +86 10-6276 7155
Email: [hidden email]
http://dx.plos.org/10.1371/journal.pone.0040003

Alberto Diaspro

Re: Light reading on optical nanoscopy

In reply to this post by Guy Cox-2

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

i propose confining this discussion to the far-field...what about the ultramicroscope?
Siedentopf, H., & Zsigmondy, R. (1902). Uber Sichtbarmachung und Größenbestimmung ultramikoskopischer Teilchen, mit besonderer Anwendung auf Goldrubingläser. Annalen der Physik, 315(1), 1–39.
Il giorno 23/ago/2012, alle ore 10:17, Guy Cox <[hidden email]> ha scritto:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Apart from Mark's suggestions, Synge proposed near-field microscopy in 1928 (Philosophical Magazine 6, 356). It was first demonstrated in visible light in 1984 (D.W. Pohl, W. Denk, and M. Lanz (1984). "Optical stethoscopy: Image recording with resolution λ/20". Appl. Phys. Lett. 44 (7): 651.) though it had been achieved in the microwave region as early as 1972. This is all way before the concept of STED had even been proposed (1994) let alone demonstrated (2002).
>
> It's not fair to say that only 'unlimited' methods are true super-resolution. Both 4-pi (proposed by Cremer & Cremer in 1971) and linear structured illumination are generally regarded (and marketed) as super-resolution. Linear structured illumination was demonstrated two years before STED (Gustafsson, M.G.L. 2000 Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. Journal of Microscopy 198 (2) , pp. 82-87). I5 microscopy, another technique offering substantial but not unlimited super-resolution, was achieved even earlier (Gustafsson, Agard & Sedat, Journal of Microscopy, 195, 10-16, 1999).
>
> Guy
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox 2nd edition, 2012 CRC Press
> http://www.guycox.com/optical.htm
> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Aust. Centre for Microscopy & Microanalysis, F09,
> University of Sydney, NSW 2006
> ______________________________________________
> Phone +61 2 9351 3176 Fax +61 2 9351 7682
> Mobile 0413 281 861
> ______________________________________________
> http://www.guycox.net
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Peng Xi
> Sent: Thursday, 23 August 2012 4:59 PM
> To: [hidden email]
> Subject: Re: Light reading on optical nanoscopy
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear Mark,
> I am not sure what you mean by "well before". Super-resolution is a
> term that usually refers to techniques that provides theoretically
> 'infinitely small' resolution, or down to single molecule size. That's why
> although confocal is already better (1.4x better) in resolution, it is
> generally not treated as super-resolution. And so to multiphoton microscopy.
> If you have a better candidate on inventing super-resolution, please let
> me and everybody know. I am sure that people are keen to know this.
>
>
> Sincerely,
> Peng Xi
> Ph. D. Associate Professor
> Dept. of Biomedical Engineering, College of Engineering
> Peking University, Beijing, China
> Tel: +86 10-6276 7155
> Email: [hidden email]
>
>
> On Thu, Aug 23, 2012 at 11:51 AM, Mark Cannell
> <[hidden email]>wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Hmm, A very myopic blog on a subject with a rich past. It was appreciated
>> that the Abbe 'limit' was not a limit well before Stephan Hell's work.
>> Suggest you might like to research the subject matter a bit deeper?
>>
>> Cheers
>>
>> PS I hope others don't start advertising their 'blogs' on this list, we
>> ban commercial 'blogs', perhaps this should be extend?
>>
>>
>> On 23/08/2012, at 1:32 AM, Peng Xi <[hidden email]> wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Dear List,
>>> I am blogging on optical nanoscopy, in a very casual mode.
>>>
>> http://xipeng.wordpress.com/2012/08/16/how-optical-super-resolution-is-achieved-1/
>>> It is originally written in Chinese, after I gave a related plenary
>>> talk in May 2012. Last talk, in the noon time. And the audiences were
>> from
>>> all sorts of disciplines, from fresh graduate students to renowned
>>> professors. Therefore, I decided to make the talk interesting, and easy
>> to
>>> follow by everyone. It turns out to be very successful -- much better
>> than
>>> equations and diagrams. So, I decide to broadcast it by blogging. :)
>>> Hope you like it.
>>>
>>> Sincerely,
>>> Peng Xi
>>> Ph. D. Associate Professor
>>> Dept. of Biomedical Engineering, College of Engineering
>>> Peking University, Beijing, China
>>> Tel: +86 10-6276 7155
>>> Email: [hidden email]
>>
>> Mark B. Cannell Ph.D. FRSNZ
>> Professor of Cardiac Cell Biology
>> School of Physiology& Pharmacology
>> Medical Sciences Building
>> University of Bristol
>> Bristol
>> BS8 1TD UK
>>
>> [hidden email]
>>

Peng Xi-2

Re: Light reading on optical nanoscopy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Oh my dear, I will be driven crazy Alby... I cannot read Germany. But I
will surely list it there. :)
Peng

On Thu, Aug 23, 2012 at 6:36 PM, Alberto Diaspro <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> i propose confining this discussion to the far-field...what about the
> ultramicroscope?
> Siedentopf, H., & Zsigmondy, R. (1902). Uber Sichtbarmachung und
> Größenbestimmung ultramikoskopischer Teilchen, mit besonderer Anwendung auf
> Goldrubingläser. Annalen der Physik, 315(1), 1–39.
> Il giorno 23/ago/2012, alle ore 10:17, Guy Cox <[hidden email]> ha
> scritto:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Apart from Mark's suggestions, Synge proposed near-field microscopy in
> 1928 (Philosophical Magazine 6, 356). It was first demonstrated in visible
> light in 1984 (D.W. Pohl, W. Denk, and M. Lanz (1984). "Optical
> stethoscopy: Image recording with resolution λ/20". Appl. Phys. Lett. 44
> (7): 651.) though it had been achieved in the microwave region as early as
> 1972. This is all way before the concept of STED had even been proposed
> (1994) let alone demonstrated (2002).
> >
> > It's not fair to say that only 'unlimited' methods are true
> super-resolution. Both 4-pi (proposed by Cremer & Cremer in 1971) and
> linear structured illumination are generally regarded (and marketed) as
> super-resolution. Linear structured illumination was demonstrated two
> years before STED (Gustafsson, M.G.L. 2000 Surpassing the lateral
> resolution limit by a factor of two using structured illumination
> microscopy. Journal of Microscopy 198 (2) , pp. 82-87). I5 microscopy,
> another technique offering substantial but not unlimited super-resolution,
> was achieved even earlier (Gustafsson, Agard & Sedat, Journal of
> Microscopy, 195, 10-16, 1999).
> >
> > Guy
> >
> > Optical Imaging Techniques in Cell Biology
> > by Guy Cox 2nd edition, 2012 CRC Press
> > http://www.guycox.com/optical.htm
> > ______________________________________________
> > Associate Professor Guy Cox, MA, DPhil(Oxon)
> > Aust. Centre for Microscopy & Microanalysis, F09,
> > University of Sydney, NSW 2006
> > ______________________________________________
> > Phone +61 2 9351 3176 Fax +61 2 9351 7682
> > Mobile 0413 281 861
> > ______________________________________________
> > http://www.guycox.net
> >
> >
> >
> > -----Original Message-----
> > From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Peng Xi
> > Sent: Thursday, 23 August 2012 4:59 PM
> > To: [hidden email]
> > Subject: Re: Light reading on optical nanoscopy
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Dear Mark,
> > I am not sure what you mean by "well before". Super-resolution is a
> > term that usually refers to techniques that provides theoretically
> > 'infinitely small' resolution, or down to single molecule size. That's
> why
> > although confocal is already better (1.4x better) in resolution, it is
> > generally not treated as super-resolution. And so to multiphoton
> microscopy.
> > If you have a better candidate on inventing super-resolution, please
> let
> > me and everybody know. I am sure that people are keen to know this.
> >
> >
> > Sincerely,
> > Peng Xi
> > Ph. D. Associate Professor
> > Dept. of Biomedical Engineering, College of Engineering
> > Peking University, Beijing, China
> > Tel: +86 10-6276 7155
> > Email: [hidden email]
> >
> >
> > On Thu, Aug 23, 2012 at 11:51 AM, Mark Cannell
> > <[hidden email]>wrote:
> >
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >> *****
> >>
> >> Hmm, A very myopic blog on a subject with a rich past. It was
> appreciated
> >> that the Abbe 'limit' was not a limit well before Stephan Hell's work.
> >> Suggest you might like to research the subject matter a bit deeper?
> >>
> >> Cheers
> >>
> >> PS I hope others don't start advertising their 'blogs' on this list, we
> >> ban commercial 'blogs', perhaps this should be extend?
> >>
> >>
> >> On 23/08/2012, at 1:32 AM, Peng Xi <[hidden email]> wrote:
> >>
> >>> *****
> >>> To join, leave or search the confocal microscopy listserv, go to:
> >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >>> *****
> >>>
> >>> Dear List,
> >>> I am blogging on optical nanoscopy, in a very casual mode.
> >>>
> >>
> http://xipeng.wordpress.com/2012/08/16/how-optical-super-resolution-is-achieved-1/
> >>> It is originally written in Chinese, after I gave a related plenary
> >>> talk in May 2012. Last talk, in the noon time. And the audiences were
> >> from
> >>> all sorts of disciplines, from fresh graduate students to renowned
> >>> professors. Therefore, I decided to make the talk interesting, and easy
> >> to
> >>> follow by everyone. It turns out to be very successful -- much better
> >> than
> >>> equations and diagrams. So, I decide to broadcast it by blogging. :)
> >>> Hope you like it.
> >>>
> >>> Sincerely,
> >>> Peng Xi
> >>> Ph. D. Associate Professor
> >>> Dept. of Biomedical Engineering, College of Engineering
> >>> Peking University, Beijing, China
> >>> Tel: +86 10-6276 7155
> >>> Email: [hidden email]
> >>
> >> Mark B. Cannell Ph.D. FRSNZ
> >> Professor of Cardiac Cell Biology
> >> School of Physiology& Pharmacology
> >> Medical Sciences Building
> >> University of Bristol
> >> Bristol
> >> BS8 1TD UK
> >>
> >> [hidden email]
> >>
>

Alberto Diaspro

Re: Light reading on optical nanoscopy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

English version should be available...please, let me check

Il giorno 23/ago/2012, alle ore 13:17, Peng Xi <[hidden email]> ha scritto:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Oh my dear, I will be driven crazy Alby... I cannot read Germany. But I
> will surely list it there. :)
> Peng
>
> On Thu, Aug 23, 2012 at 6:36 PM, Alberto Diaspro <[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> i propose confining this discussion to the far-field...what about the
>> ultramicroscope?
>> Siedentopf, H., & Zsigmondy, R. (1902). Uber Sichtbarmachung und
>> Größenbestimmung ultramikoskopischer Teilchen, mit besonderer Anwendung auf
>> Goldrubingläser. Annalen der Physik, 315(1), 1–39.
>> Il giorno 23/ago/2012, alle ore 10:17, Guy Cox <[hidden email]> ha
>> scritto:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Apart from Mark's suggestions, Synge proposed near-field microscopy in
>> 1928 (Philosophical Magazine 6, 356). It was first demonstrated in visible
>> light in 1984 (D.W. Pohl, W. Denk, and M. Lanz (1984). "Optical
>> stethoscopy: Image recording with resolution λ/20". Appl. Phys. Lett. 44
>> (7): 651.) though it had been achieved in the microwave region as early as
>> 1972. This is all way before the concept of STED had even been proposed
>> (1994) let alone demonstrated (2002).
>>>
>>> It's not fair to say that only 'unlimited' methods are true
>> super-resolution. Both 4-pi (proposed by Cremer & Cremer in 1971) and
>> linear structured illumination are generally regarded (and marketed) as
>> super-resolution. Linear structured illumination was demonstrated two
>> years before STED (Gustafsson, M.G.L. 2000 Surpassing the lateral
>> resolution limit by a factor of two using structured illumination
>> microscopy. Journal of Microscopy 198 (2) , pp. 82-87). I5 microscopy,
>> another technique offering substantial but not unlimited super-resolution,
>> was achieved even earlier (Gustafsson, Agard & Sedat, Journal of
>> Microscopy, 195, 10-16, 1999).
>>>
>>> Guy
>>>
>>> Optical Imaging Techniques in Cell Biology
>>> by Guy Cox 2nd edition, 2012 CRC Press
>>> http://www.guycox.com/optical.htm
>>> ______________________________________________
>>> Associate Professor Guy Cox, MA, DPhil(Oxon)
>>> Aust. Centre for Microscopy & Microanalysis, F09,
>>> University of Sydney, NSW 2006
>>> ______________________________________________
>>> Phone +61 2 9351 3176 Fax +61 2 9351 7682
>>> Mobile 0413 281 861
>>> ______________________________________________
>>> http://www.guycox.net
>>>
>>>
>>>
>>> -----Original Message-----
>>> From: Confocal Microscopy List [mailto:[hidden email]]
>> On Behalf Of Peng Xi
>>> Sent: Thursday, 23 August 2012 4:59 PM
>>> To: [hidden email]
>>> Subject: Re: Light reading on optical nanoscopy
>>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Dear Mark,
>>> I am not sure what you mean by "well before". Super-resolution is a
>>> term that usually refers to techniques that provides theoretically
>>> 'infinitely small' resolution, or down to single molecule size. That's
>> why
>>> although confocal is already better (1.4x better) in resolution, it is
>>> generally not treated as super-resolution. And so to multiphoton
>> microscopy.
>>> If you have a better candidate on inventing super-resolution, please
>> let
>>> me and everybody know. I am sure that people are keen to know this.
>>>
>>>
>>> Sincerely,
>>> Peng Xi
>>> Ph. D. Associate Professor
>>> Dept. of Biomedical Engineering, College of Engineering
>>> Peking University, Beijing, China
>>> Tel: +86 10-6276 7155
>>> Email: [hidden email]
>>>
>>>
>>> On Thu, Aug 23, 2012 at 11:51 AM, Mark Cannell
>>> <[hidden email]>wrote:
>>>
>>>> *****
>>>> To join, leave or search the confocal microscopy listserv, go to:
>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>>> *****
>>>>
>>>> Hmm, A very myopic blog on a subject with a rich past. It was
>> appreciated
>>>> that the Abbe 'limit' was not a limit well before Stephan Hell's work.
>>>> Suggest you might like to research the subject matter a bit deeper?
>>>>
>>>> Cheers
>>>>
>>>> PS I hope others don't start advertising their 'blogs' on this list, we
>>>> ban commercial 'blogs', perhaps this should be extend?
>>>>
>>>>
>>>> On 23/08/2012, at 1:32 AM, Peng Xi <[hidden email]> wrote:
>>>>
>>>>> *****
>>>>> To join, leave or search the confocal microscopy listserv, go to:
>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>>>> *****
>>>>>
>>>>> Dear List,
>>>>> I am blogging on optical nanoscopy, in a very casual mode.
>>>>>
>>>>
>> http://xipeng.wordpress.com/2012/08/16/how-optical-super-resolution-is-achieved-1/
>>>>> It is originally written in Chinese, after I gave a related plenary
>>>>> talk in May 2012. Last talk, in the noon time. And the audiences were
>>>> from
>>>>> all sorts of disciplines, from fresh graduate students to renowned
>>>>> professors. Therefore, I decided to make the talk interesting, and easy
>>>> to
>>>>> follow by everyone. It turns out to be very successful -- much better
>>>> than
>>>>> equations and diagrams. So, I decide to broadcast it by blogging. :)
>>>>> Hope you like it.
>>>>>
>>>>> Sincerely,
>>>>> Peng Xi
>>>>> Ph. D. Associate Professor
>>>>> Dept. of Biomedical Engineering, College of Engineering
>>>>> Peking University, Beijing, China
>>>>> Tel: +86 10-6276 7155
>>>>> Email: [hidden email]
>>>>
>>>> Mark B. Cannell Ph.D. FRSNZ
>>>> Professor of Cardiac Cell Biology
>>>> School of Physiology& Pharmacology
>>>> Medical Sciences Building
>>>> University of Bristol
>>>> Bristol
>>>> BS8 1TD UK
>>>>
>>>> [hidden email]
>>>>
>>

Martin Wessendorf-2

Re: Light reading on optical nanoscopy

In reply to this post by Mark Cannell-2

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

On 8/22/2012 10:51 PM, Mark Cannell wrote:
> PS I hope others don't start advertising their 'blogs' on this list, we ban commercial 'blogs', perhaps this should be extend?

Links to blogs--by commercial interests or individuals--are welcome on
this list as long as the blog is relevant to microscopy and the posting
is concise.

The policy for commercial postings can be found at:
http://lists.umn.edu/cgi-bin/wa?A2=ind1203&L=CONFOCALMICROSCOPY&P=R2835
for those who are interested.

Thanks to all for your participation in this list! I need to check my
records, but I think that it's now been twenty years since these
conversations started. If Bob Summers is listening, we all owe him a
big "Thank you" for that!

Martin Wessendorf
listowner
--
Martin Wessendorf, Ph.D. office: (612) 626-0145
Assoc Prof, Dept Neuroscience lab: (612) 624-2991
University of Minnesota Preferred FAX: (612) 624-8118
6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009
Minneapolis, MN 55455 e-mail: [hidden email]

Martin Wessendorf-2

Re: Light reading on optical nanoscopy

In reply to this post by Peng Xi-2

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

On 8/22/2012 10:51 PM, Mark Cannell wrote:
> PS I hope others don't start advertising their 'blogs' on this list, we ban commercial 'blogs', perhaps this should be extend?

Just so this is clear, links to blogs--by commercial interests or
individuals--are welcome on this list as long as the blog is relevant to
microscopy and the posting is concise.

The policy for commercial postings can be found at:
http://lists.umn.edu/cgi-bin/wa?A2=ind1203&L=CONFOCALMICROSCOPY&P=R2835
for those who are interested.

Thanks to all for your participation in the list. It's now been over
twenty years since these conversations started. If Bob Summers (--the
originator of this group) is listening, we all owe him a huge "Thank
you" for that!

Martin Wessendorf

--
Martin Wessendorf, Ph.D. office: (612) 626-0145
Assoc Prof, Dept Neuroscience lab: (612) 624-2991
University of Minnesota Preferred FAX: (612) 624-8118
6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009
Minneapolis, MN 55455 e-mail: [hidden email]

JOEL B. SHEFFIELD

Re: Light reading on optical nanoscopy

In reply to this post by Peng Xi-2

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi All,

I think that it is also appropriate to include some of the side-band
enhancement stuff that Bob Allen did, leading to a major expansion of our
understanding of microtubules, and Shinya Inoue's work with polarization. --

- Allen, R. D., Allen, N. S. & Travis, J. L. Video-enhanced contrast,
differential interference contrast (AVEC–DIC) microscopy: a new method
capable of analyzing microtubule-related motility in the reticulopodial
network of *Allogromia laticollaris*. Cell Motil. 1, 291–302 (1981) |
Article <http://dx.doi.org/10.1002/cm.970010303> |
PubMed<http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?holding=npg&cmd=Retrieve&db=PubMed&list_uids=7348605&dopt=Abstract>
| ISI<http://links.isiglobalnet2.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=Nature&SrcApp=Nature&DestLinkType=FullRecord&KeyUT=A1981LU28900002&DestApp=WOS_CPL>
| ChemPort<http://chemport.cas.org/cgi-bin/sdcgi?APP=ftslink&action=reflink&origin=npg&version=1.0&coi=1:STN:280:BiyD2sjgvVM%3D&pissn=%7BprintIssn%7D&pyear=2008&md5=984030314c7010cb94fdb99bc17a7fae>
|
-
-
- Inoué, S. Video image processing greatly enhances contrast, quality,
and speed in polarization-based microscopy. J. Cell Biol. 89, 346–356 (
1981) | Article <http://dx.doi.org/10.1083/jcb.89.2.346> |
PubMed<http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?holding=npg&cmd=Retrieve&db=PubMed&list_uids=6788777&dopt=Abstract>
| ISI<http://links.isiglobalnet2.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=Nature&SrcApp=Nature&DestLinkType=FullRecord&KeyUT=A1981LM51800021&DestApp=WOS_CPL>
| ChemPort<http://chemport.cas.org/cgi-bin/sdcgi?APP=ftslink&action=reflink&origin=npg&version=1.0&coi=1:STN:280:Bi6B3sbnsVQ%3D&pissn=%7BprintIssn%7D&pyear=2008&md5=59167257773765ac393b282b65e22a65>
|

Joel

On Thu, Aug 23, 2012 at 2:59 AM, Peng Xi <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear Mark,
> I am not sure what you mean by "well before". Super-resolution is a
> term that usually refers to techniques that provides theoretically
> 'infinitely small' resolution, or down to single molecule size. That's why
> although confocal is already better (1.4x better) in resolution, it is
> generally not treated as super-resolution. And so to multiphoton
> microscopy.
> If you have a better candidate on inventing super-resolution, please let
> me and everybody know. I am sure that people are keen to know this.
>
>
> Sincerely,
> Peng Xi
> Ph. D. Associate Professor
> Dept. of Biomedical Engineering, College of Engineering
> Peking University, Beijing, China
> Tel: +86 10-6276 7155
> Email: [hidden email]
>
>
> On Thu, Aug 23, 2012 at 11:51 AM, Mark Cannell
> <[hidden email]>wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Hmm, A very myopic blog on a subject with a rich past. It was
> appreciated
> > that the Abbe 'limit' was not a limit well before Stephan Hell's work.
> > Suggest you might like to research the subject matter a bit deeper?
> >
> > Cheers
> >
> > PS I hope others don't start advertising their 'blogs' on this list, we
> > ban commercial 'blogs', perhaps this should be extend?
> >
> >
> > On 23/08/2012, at 1:32 AM, Peng Xi <[hidden email]> wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > *****
> > >
> > > Dear List,
> > > I am blogging on optical nanoscopy, in a very casual mode.
> > >
> >
> http://xipeng.wordpress.com/2012/08/16/how-optical-super-resolution-is-achieved-1/
> > > It is originally written in Chinese, after I gave a related plenary
> > > talk in May 2012. Last talk, in the noon time. And the audiences were
> > from
> > > all sorts of disciplines, from fresh graduate students to renowned
> > > professors. Therefore, I decided to make the talk interesting, and easy
> > to
> > > follow by everyone. It turns out to be very successful -- much better
> > than
> > > equations and diagrams. So, I decide to broadcast it by blogging. :)
> > > Hope you like it.
> > >
> > > Sincerely,
> > > Peng Xi
> > > Ph. D. Associate Professor
> > > Dept. of Biomedical Engineering, College of Engineering
> > > Peking University, Beijing, China
> > > Tel: +86 10-6276 7155
> > > Email: [hidden email]
> >
> > Mark B. Cannell Ph.D. FRSNZ
> > Professor of Cardiac Cell Biology
> > School of Physiology& Pharmacology
> > Medical Sciences Building
> > University of Bristol
> > Bristol
> > BS8 1TD UK
> >
> > [hidden email]
> >
>

--

Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [hidden email]
URL: http://astro.temple.edu/~jbs

Peng Xi-2

Re: Light reading on optical nanoscopy

In reply to this post by Guy Cox-2

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear Guy,
Regarding the first demonstration of STED, I think you can read this
paper, in which far-field STED demonstrated 106nm resolution in 1999:
Thomas A. Klar and Stefan W. Hell, Subdiffraction resolution in far-field
fluorescence microscopy PTICS LETTERS / Vol. 24, No. 14 / 954-956, 1999
In fact, the concept of STED can also be regarded as an extension of
confocal: in confocal we set the pinhole at detector, which effectively
being imaged onto the focal spot, only also diffraction limited. If the
image of the pinhole is plotted, it will be precisely a doughnut! As STED
can brings a much "harder" doughnut, this achieves not just 1.4x resolution
enhancing, but to theoretically infinity thanks to the saturation
depletion.
It is very hard to do this optical modulation. On the contrary,
near-field optics can easily achieve the same narrow doughnut, by just
limiting the tip size.
This discussion will be in the upcoming CRC book I am editing. (Thank
you for your nice comments on the book evaluation!)
Peng

On Thu, Aug 23, 2012 at 4:17 PM, Guy Cox <[hidden email]> wrote:

Mark Cannell-2

Re: Light reading on optical nanoscopy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear Peng,

Maybe this is a communication/language problem but I don't think the plotted image of the pinhole is a 'doughnut'. A doughnut is a ring, and is similar to an annulus, but pinholes are just holes... Without a confocal pinhole in a STED system the illumination pattern does not control out of focus light well, the depletion zone has a complex extended 3D structure of limited extent (it does not look much like a doughnut IMHO). Also, I think the near field probe response function does not look like a STED excitation field either.

On a connected issue, the structured light approach is different as it is not a scanning point detector, but whether this can be called wide field super resolution is not clear to me as a part of the field is suppressed in a time dependent way. I'd be interested on expert opinions on this question of definition…

Cheers Mark

On 24/08/2012, at 1:36 AM, Peng Xi <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear Guy,
> Regarding the first demonstration of STED, I think you can read this
> paper, in which far-field STED demonstrated 106nm resolution in 1999:
> Thomas A. Klar and Stefan W. Hell, Subdiffraction resolution in far-field
> fluorescence microscopy PTICS LETTERS / Vol. 24, No. 14 / 954-956, 1999
> In fact, the concept of STED can also be regarded as an extension of
> confocal: in confocal we set the pinhole at detector, which effectively
> being imaged onto the focal spot, only also diffraction limited. If the
> image of the pinhole is plotted, it will be precisely a doughnut! As STED
> can brings a much "harder" doughnut, this achieves not just 1.4x resolution
> enhancing, but to theoretically infinity thanks to the saturation
> depletion.
> It is very hard to do this optical modulation. On the contrary,
> near-field optics can easily achieve the same narrow doughnut, by just
> limiting the tip size.
> This discussion will be in the upcoming CRC book I am editing. (Thank
> you for your nice comments on the book evaluation!)
> Peng
>
>
> On Thu, Aug 23, 2012 at 4:17 PM, Guy Cox <[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Apart from Mark's suggestions, Synge proposed near-field microscopy in
>> 1928 (Philosophical Magazine 6, 356). It was first demonstrated in visible
>> light in 1984 (D.W. Pohl, W. Denk, and M. Lanz (1984). "Optical
>> stethoscopy: Image recording with resolution λ/20". Appl. Phys. Lett. 44
>> (7): 651.) though it had been achieved in the microwave region as early as
>> 1972. This is all way before the concept of STED had even been proposed
>> (1994) let alone demonstrated (2002).
>>
>> It's not fair to say that only 'unlimited' methods are true
>> super-resolution. Both 4-pi (proposed by Cremer & Cremer in 1971) and
>> linear structured illumination are generally regarded (and marketed) as
>> super-resolution. Linear structured illumination was demonstrated two
>> years before STED (Gustafsson, M.G.L. 2000 Surpassing the lateral
>> resolution limit by a factor of two using structured illumination
>> microscopy. Journal of Microscopy 198 (2) , pp. 82-87). I5 microscopy,
>> another technique offering substantial but not unlimited super-resolution,
>> was achieved even earlier (Gustafsson, Agard & Sedat, Journal of
>> Microscopy, 195, 10-16, 1999).
>>
>> Guy
>>
>> Optical Imaging Techniques in Cell Biology
>> by Guy Cox 2nd edition, 2012 CRC Press
>> http://www.guycox.com/optical.htm
>> ______________________________________________
>> Associate Professor Guy Cox, MA, DPhil(Oxon)
>> Aust. Centre for Microscopy & Microanalysis, F09,
>> University of Sydney, NSW 2006
>> ______________________________________________
>> Phone +61 2 9351 3176 Fax +61 2 9351 7682
>> Mobile 0413 281 861
>> ______________________________________________
>> http://www.guycox.net
>>
>>
>>
>> -----Original Message-----
>> From: Confocal Microscopy List [mailto:[hidden email]]
>> On Behalf Of Peng Xi
>> Sent: Thursday, 23 August 2012 4:59 PM
>> To: [hidden email]
>> Subject: Re: Light reading on optical nanoscopy
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Dear Mark,
>> I am not sure what you mean by "well before". Super-resolution is a
>> term that usually refers to techniques that provides theoretically
>> 'infinitely small' resolution, or down to single molecule size. That's why
>> although confocal is already better (1.4x better) in resolution, it is
>> generally not treated as super-resolution. And so to multiphoton
>> microscopy.
>> If you have a better candidate on inventing super-resolution, please let
>> me and everybody know. I am sure that people are keen to know this.
>>
>>
>> Sincerely,
>> Peng Xi
>> Ph. D. Associate Professor
>> Dept. of Biomedical Engineering, College of Engineering
>> Peking University, Beijing, China
>> Tel: +86 10-6276 7155
>> Email: [hidden email]
>>
>>
>> On Thu, Aug 23, 2012 at 11:51 AM, Mark Cannell
>> <[hidden email]>wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Hmm, A very myopic blog on a subject with a rich past. It was
>> appreciated
>>> that the Abbe 'limit' was not a limit well before Stephan Hell's work.
>>> Suggest you might like to research the subject matter a bit deeper?
>>>
>>> Cheers
>>>
>>> PS I hope others don't start advertising their 'blogs' on this list, we
>>> ban commercial 'blogs', perhaps this should be extend?
>>>
>>>
>>> On 23/08/2012, at 1:32 AM, Peng Xi <[hidden email]> wrote:
>>>
>>>> *****
>>>> To join, leave or search the confocal microscopy listserv, go to:
>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>>> *****
>>>>
>>>> Dear List,
>>>> I am blogging on optical nanoscopy, in a very casual mode.
>>>>
>>>
>> http://xipeng.wordpress.com/2012/08/16/how-optical-super-resolution-is-achieved-1/
>>>> It is originally written in Chinese, after I gave a related plenary
>>>> talk in May 2012. Last talk, in the noon time. And the audiences were
>>> from
>>>> all sorts of disciplines, from fresh graduate students to renowned
>>>> professors. Therefore, I decided to make the talk interesting, and easy
>>> to
>>>> follow by everyone. It turns out to be very successful -- much better
>>> than
>>>> equations and diagrams. So, I decide to broadcast it by blogging. :)
>>>> Hope you like it.
>>>>
>>>> Sincerely,
>>>> Peng Xi
>>>> Ph. D. Associate Professor
>>>> Dept. of Biomedical Engineering, College of Engineering
>>>> Peking University, Beijing, China
>>>> Tel: +86 10-6276 7155
>>>> Email: [hidden email]
>>>
>>> Mark B. Cannell Ph.D. FRSNZ
>>> Professor of Cardiac Cell Biology
>>> School of Physiology& Pharmacology
>>> Medical Sciences Building
>>> University of Bristol
>>> Bristol
>>> BS8 1TD UK
>>>
>>> [hidden email]
>>>
>>

Mark B. Cannell Ph.D. FRSNZ
Professor of Cardiac Cell Biology
School of Physiology& Pharmacology
Medical Sciences Building
University of Bristol
Bristol
BS8 1TD UK

[hidden email]

Guy Cox-2

Re: Light reading on optical nanoscopy

Maybe you've become Americanized, Mark. An English doughnut is spherical, with jam in the middle. Translating this into a psf is an exercise I leave to others!

More seriously, I agree that you can't call structured illumination widefield. The super-resolution depends on restricting the field of view, as in confocal, Toraldo filters, etc.

Guy

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Mark Cannell
Sent: Friday, 24 August 2012 6:39 PM
To: [hidden email]
Subject: Re: Light reading on optical nanoscopy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear Peng,

Maybe this is a communication/language problem but I don't think the plotted image of the pinhole is a 'doughnut'. A doughnut is a ring, and is similar to an annulus, but pinholes are just holes... Without a confocal pinhole in a STED system the illumination pattern does not control out of focus light well, the depletion zone has a complex extended 3D structure of limited extent (it does not look much like a doughnut IMHO). Also, I think the near field probe response function does not look like a STED excitation field either.

On a connected issue, the structured light approach is different as it is not a scanning point detector, but whether this can be called wide field super resolution is not clear to me as a part of the field is suppressed in a time dependent way. I'd be interested on expert opinions on this question of definition…

Cheers Mark

On 24/08/2012, at 1:36 AM, Peng Xi <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear Guy,
> Regarding the first demonstration of STED, I think you can read
> this paper, in which far-field STED demonstrated 106nm resolution in 1999:
> Thomas A. Klar and Stefan W. Hell, Subdiffraction resolution in
> far-field fluorescence microscopy PTICS LETTERS / Vol. 24, No. 14 / 954-956, 1999
> In fact, the concept of STED can also be regarded as an extension
> of
> confocal: in confocal we set the pinhole at detector, which
> effectively being imaged onto the focal spot, only also diffraction
> limited. If the image of the pinhole is plotted, it will be precisely
> a doughnut! As STED can brings a much "harder" doughnut, this
> achieves not just 1.4x resolution enhancing, but to theoretically
> infinity thanks to the saturation depletion.
> It is very hard to do this optical modulation. On the contrary,
> near-field optics can easily achieve the same narrow doughnut, by just
> limiting the tip size.
> This discussion will be in the upcoming CRC book I am editing.
> (Thank you for your nice comments on the book evaluation!) Peng
>
>
> On Thu, Aug 23, 2012 at 4:17 PM, Guy Cox <[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Apart from Mark's suggestions, Synge proposed near-field microscopy
>> in
>> 1928 (Philosophical Magazine 6, 356). It was first demonstrated in
>> visible light in 1984 (D.W. Pohl, W. Denk, and M. Lanz (1984).
>> "Optical
>> stethoscopy: Image recording with resolution λ/20". Appl. Phys. Lett.
>> 44
>> (7): 651.) though it had been achieved in the microwave region as
>> early as 1972. This is all way before the concept of STED had even
>> been proposed
>> (1994) let alone demonstrated (2002).
>>
>> It's not fair to say that only 'unlimited' methods are true
>> super-resolution. Both 4-pi (proposed by Cremer & Cremer in 1971)
>> and linear structured illumination are generally regarded (and
>> marketed) as super-resolution. Linear structured illumination was
>> demonstrated two years before STED (Gustafsson, M.G.L. 2000
>> Surpassing the lateral resolution limit by a factor of two using
>> structured illumination microscopy. Journal of Microscopy 198 (2) ,
>> pp. 82-87). I5 microscopy, another technique offering substantial
>> but not unlimited super-resolution, was achieved even earlier
>> (Gustafsson, Agard & Sedat, Journal of Microscopy, 195, 10-16, 1999).
>>
>> Guy
>>
>> Optical Imaging Techniques in Cell Biology
>> by Guy Cox 2nd edition, 2012 CRC Press
>> http://www.guycox.com/optical.htm
>> ______________________________________________
>> Associate Professor Guy Cox, MA, DPhil(Oxon) Aust. Centre for
>> Microscopy & Microanalysis, F09, University of Sydney, NSW 2006
>> ______________________________________________
>> Phone +61 2 9351 3176 Fax +61 2 9351 7682
>> Mobile 0413 281 861
>> ______________________________________________
>> http://www.guycox.net
>>
>>
>>
>> -----Original Message-----
>> From: Confocal Microscopy List
>> [mailto:[hidden email]]
>> On Behalf Of Peng Xi
>> Sent: Thursday, 23 August 2012 4:59 PM
>> To: [hidden email]
>> Subject: Re: Light reading on optical nanoscopy
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Dear Mark,
>> I am not sure what you mean by "well before". Super-resolution is
>> a term that usually refers to techniques that provides theoretically
>> 'infinitely small' resolution, or down to single molecule size.
>> That's why although confocal is already better (1.4x better) in
>> resolution, it is generally not treated as super-resolution. And so
>> to multiphoton microscopy.
>> If you have a better candidate on inventing super-resolution,
>> please let me and everybody know. I am sure that people are keen to know this.
>>
>>
>> Sincerely,
>> Peng Xi
>> Ph. D. Associate Professor
>> Dept. of Biomedical Engineering, College of Engineering Peking
>> University, Beijing, China
>> Tel: +86 10-6276 7155
>> Email: [hidden email]
>>
>>
>> On Thu, Aug 23, 2012 at 11:51 AM, Mark Cannell
>> <[hidden email]>wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Hmm, A very myopic blog on a subject with a rich past. It was
>> appreciated
>>> that the Abbe 'limit' was not a limit well before Stephan Hell's work.
>>> Suggest you might like to research the subject matter a bit deeper?
>>>
>>> Cheers
>>>
>>> PS I hope others don't start advertising their 'blogs' on this
>>> list, we ban commercial 'blogs', perhaps this should be extend?
>>>
>>>
>>> On 23/08/2012, at 1:32 AM, Peng Xi <[hidden email]> wrote:
>>>
>>>> *****
>>>> To join, leave or search the confocal microscopy listserv, go to:
>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>>> *****
>>>>
>>>> Dear List,
>>>> I am blogging on optical nanoscopy, in a very casual mode.
>>>>
>>>
>> http://xipeng.wordpress.com/2012/08/16/how-optical-super-resolution-i
>> s-achieved-1/
>>>> It is originally written in Chinese, after I gave a related
>>>> plenary talk in May 2012. Last talk, in the noon time. And the
>>>> audiences were
>>> from
>>>> all sorts of disciplines, from fresh graduate students to renowned
>>>> professors. Therefore, I decided to make the talk interesting, and
>>>> easy
>>> to
>>>> follow by everyone. It turns out to be very successful -- much
>>>> better
>>> than
>>>> equations and diagrams. So, I decide to broadcast it by blogging. :)
>>>> Hope you like it.
>>>>
>>>> Sincerely,
>>>> Peng Xi
>>>> Ph. D. Associate Professor
>>>> Dept. of Biomedical Engineering, College of Engineering Peking
>>>> University, Beijing, China
>>>> Tel: +86 10-6276 7155
>>>> Email: [hidden email]
>>>
>>> Mark B. Cannell Ph.D. FRSNZ
>>> Professor of Cardiac Cell Biology
>>> School of Physiology& Pharmacology
>>> Medical Sciences Building
>>> University of Bristol
>>> Bristol
>>> BS8 1TD UK
>>>
>>> [hidden email]
>>>
>>

Mark B. Cannell Ph.D. FRSNZ
Professor of Cardiac Cell Biology
School of Physiology& Pharmacology
Medical Sciences Building
University of Bristol
Bristol
BS8 1TD UK

[hidden email]

Peng Xi-2

Re: Light reading on optical nanoscopy

In reply to this post by Mark Cannell-2

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear all,
New posts on PALM/STORM is on my blog now. xipeng.wordpress.com
Have a good weekend!

Sincerely,
Peng Xi
Ph. D. Associate Professor
Dept. of Biomedical Engineering, College of Engineering
Peking University, Beijing, China
Tel: +86 10-6276 7155
Email: [hidden email]

Mark Cannell-2

Re: Light reading on optical nanoscopy

In reply to this post by Guy Cox-2

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Really, if someone says it's 'doughnut shaped' you think of a sphere?

Cheers

On 24/08/2012, at 10:30 AM, Guy Cox <[hidden email]> wrote:

> Maybe you've become Americanized, Mark. An English doughnut is spherical, with jam in the middle. Translating this into a psf is an exercise I leave to others!
>
> More seriously, I agree that you can't call structured illumination widefield. The super-resolution depends on restricting the field of view, as in confocal, Toraldo filters, etc.
>
> Guy
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Mark Cannell
> Sent: Friday, 24 August 2012 6:39 PM
> To: [hidden email]
> Subject: Re: Light reading on optical nanoscopy
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear Peng,
>
> Maybe this is a communication/language problem but I don't think the plotted image of the pinhole is a 'doughnut'. A doughnut is a ring, and is similar to an annulus, but pinholes are just holes... Without a confocal pinhole in a STED system the illumination pattern does not control out of focus light well, the depletion zone has a complex extended 3D structure of limited extent (it does not look much like a doughnut IMHO). Also, I think the near field probe response function does not look like a STED excitation field either.
>
> On a connected issue, the structured light approach is different as it is not a scanning point detector, but whether this can be called wide field super resolution is not clear to me as a part of the field is suppressed in a time dependent way. I'd be interested on expert opinions on this question of definition…
>
> Cheers Mark
>
>
> On 24/08/2012, at 1:36 AM, Peng Xi <[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Dear Guy,
>> Regarding the first demonstration of STED, I think you can read
>> this paper, in which far-field STED demonstrated 106nm resolution in 1999:
>> Thomas A. Klar and Stefan W. Hell, Subdiffraction resolution in
>> far-field fluorescence microscopy PTICS LETTERS / Vol. 24, No. 14 / 954-956, 1999
>> In fact, the concept of STED can also be regarded as an extension
>> of
>> confocal: in confocal we set the pinhole at detector, which
>> effectively being imaged onto the focal spot, only also diffraction
>> limited. If the image of the pinhole is plotted, it will be precisely
>> a doughnut! As STED can brings a much "harder" doughnut, this
>> achieves not just 1.4x resolution enhancing, but to theoretically
>> infinity thanks to the saturation depletion.
>> It is very hard to do this optical modulation. On the contrary,
>> near-field optics can easily achieve the same narrow doughnut, by just
>> limiting the tip size.
>> This discussion will be in the upcoming CRC book I am editing.
>> (Thank you for your nice comments on the book evaluation!) Peng
>>
>>
>> On Thu, Aug 23, 2012 at 4:17 PM, Guy Cox <[hidden email]> wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Apart from Mark's suggestions, Synge proposed near-field microscopy
>>> in
>>> 1928 (Philosophical Magazine 6, 356). It was first demonstrated in
>>> visible light in 1984 (D.W. Pohl, W. Denk, and M. Lanz (1984).
>>> "Optical
>>> stethoscopy: Image recording with resolution λ/20". Appl. Phys. Lett.
>>> 44
>>> (7): 651.) though it had been achieved in the microwave region as
>>> early as 1972. This is all way before the concept of STED had even
>>> been proposed
>>> (1994) let alone demonstrated (2002).
>>>
>>> It's not fair to say that only 'unlimited' methods are true
>>> super-resolution. Both 4-pi (proposed by Cremer & Cremer in 1971)
>>> and linear structured illumination are generally regarded (and
>>> marketed) as super-resolution. Linear structured illumination was
>>> demonstrated two years before STED (Gustafsson, M.G.L. 2000
>>> Surpassing the lateral resolution limit by a factor of two using
>>> structured illumination microscopy. Journal of Microscopy 198 (2) ,
>>> pp. 82-87). I5 microscopy, another technique offering substantial
>>> but not unlimited super-resolution, was achieved even earlier
>>> (Gustafsson, Agard & Sedat, Journal of Microscopy, 195, 10-16, 1999).
>>>
>>> Guy
>>>
>>> Optical Imaging Techniques in Cell Biology
>>> by Guy Cox 2nd edition, 2012 CRC Press
>>> http://www.guycox.com/optical.htm
>>> ______________________________________________
>>> Associate Professor Guy Cox, MA, DPhil(Oxon) Aust. Centre for
>>> Microscopy & Microanalysis, F09, University of Sydney, NSW 2006
>>> ______________________________________________
>>> Phone +61 2 9351 3176 Fax +61 2 9351 7682
>>> Mobile 0413 281 861
>>> ______________________________________________
>>> http://www.guycox.net
>>>
>>>
>>>
>>> -----Original Message-----
>>> From: Confocal Microscopy List
>>> [mailto:[hidden email]]
>>> On Behalf Of Peng Xi
>>> Sent: Thursday, 23 August 2012 4:59 PM
>>> To: [hidden email]
>>> Subject: Re: Light reading on optical nanoscopy
>>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Dear Mark,
>>> I am not sure what you mean by "well before". Super-resolution is
>>> a term that usually refers to techniques that provides theoretically
>>> 'infinitely small' resolution, or down to single molecule size.
>>> That's why although confocal is already better (1.4x better) in
>>> resolution, it is generally not treated as super-resolution. And so
>>> to multiphoton microscopy.
>>> If you have a better candidate on inventing super-resolution,
>>> please let me and everybody know. I am sure that people are keen to know this.
>>>
>>>
>>> Sincerely,
>>> Peng Xi
>>> Ph. D. Associate Professor
>>> Dept. of Biomedical Engineering, College of Engineering Peking
>>> University, Beijing, China
>>> Tel: +86 10-6276 7155
>>> Email: [hidden email]
>>>
>>>
>>> On Thu, Aug 23, 2012 at 11:51 AM, Mark Cannell
>>> <[hidden email]>wrote:
>>>
>>>> *****
>>>> To join, leave or search the confocal microscopy listserv, go to:
>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>>> *****
>>>>
>>>> Hmm, A very myopic blog on a subject with a rich past. It was
>>> appreciated
>>>> that the Abbe 'limit' was not a limit well before Stephan Hell's work.
>>>> Suggest you might like to research the subject matter a bit deeper?
>>>>
>>>> Cheers
>>>>
>>>> PS I hope others don't start advertising their 'blogs' on this
>>>> list, we ban commercial 'blogs', perhaps this should be extend?
>>>>
>>>>
>>>> On 23/08/2012, at 1:32 AM, Peng Xi <[hidden email]> wrote:
>>>>
>>>>> *****
>>>>> To join, leave or search the confocal microscopy listserv, go to:
>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>>>> *****
>>>>>
>>>>> Dear List,
>>>>> I am blogging on optical nanoscopy, in a very casual mode.
>>>>>
>>>>
>>> http://xipeng.wordpress.com/2012/08/16/how-optical-super-resolution-i
>>> s-achieved-1/
>>>>> It is originally written in Chinese, after I gave a related
>>>>> plenary talk in May 2012. Last talk, in the noon time. And the
>>>>> audiences were
>>>> from
>>>>> all sorts of disciplines, from fresh graduate students to renowned
>>>>> professors. Therefore, I decided to make the talk interesting, and
>>>>> easy
>>>> to
>>>>> follow by everyone. It turns out to be very successful -- much
>>>>> better
>>>> than
>>>>> equations and diagrams. So, I decide to broadcast it by blogging. :)
>>>>> Hope you like it.
>>>>>
>>>>> Sincerely,
>>>>> Peng Xi
>>>>> Ph. D. Associate Professor
>>>>> Dept. of Biomedical Engineering, College of Engineering Peking
>>>>> University, Beijing, China
>>>>> Tel: +86 10-6276 7155
>>>>> Email: [hidden email]
>>>>
>>>> Mark B. Cannell Ph.D. FRSNZ
>>>> Professor of Cardiac Cell Biology
>>>> School of Physiology& Pharmacology
>>>> Medical Sciences Building
>>>> University of Bristol
>>>> Bristol
>>>> BS8 1TD UK
>>>>
>>>> [hidden email]
>>>>
>>>
>
> Mark B. Cannell Ph.D. FRSNZ
> Professor of Cardiac Cell Biology
> School of Physiology& Pharmacology
> Medical Sciences Building
> University of Bristol
> Bristol
> BS8 1TD UK
>
> [hidden email]

Mark B. Cannell Ph.D. FRSNZ
Professor of Cardiac Cell Biology
School of Physiology& Pharmacology
Medical Sciences Building
University of Bristol
Bristol
BS8 1TD UK

[hidden email]

Steffen Dietzel

Re: Light reading on optical nanoscopy

In reply to this post by Peng Xi-2

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

On 23.08.2012 13:17, Peng Xi wrote:
> Oh my dear, I will be driven crazy Alby... I cannot read Germany. But I
> will surely list it there. :)
> Peng
>

Peng,

concerning ultramicroscopy, you can start with the Nobel lecture given
by Richard Zsigmondy, it can be found here:
http://www.nobelprize.org/nobel_prizes/chemistry/laureates/1925/zsigmondy-lecture.html

While this is obviously not the original paper, it has the advantage of
being in English. It also contains some images.

A better quality image of a Spaltultramikroskop than in the nobel
lecture can be found online in the paper
W. Gebhardt: Aus optischen und mechanischen Werkstätten I. In:
Zeitschrift für wissenschaftliche Mikroskopie. 24, Nr. 4, 1907, S. 396–421,
more precisely on page 405, online available here:
http://www.biodiversitylibrary.org/item/24714#page/473/mode/1up

At the time, an Ultramikroskop was any microscope that would use dark
field illumination to study particles smaller than the resolution limit
(the so-called "Ultramikronen") while the specific type developped by
Siedentopf and Zsigmondy was called a Spaltultramikroskop: It used a
slit (=Spalt) for illumination, actually quite similar to todays SPIM,
except that the readout was a darkfield image, not fluorescence. So,
don't get confused by the two terms when you brush up your German,
sometimes recent texts imply that every Ultramikroskop was a
Spaltultramikroskop but that was not the case.

Keep in mind that these microscopes did not beat the Abbe limit in terms
of resolution. You could detect and study particles smaller than the
resolution limit, yes, but you could not resolve them if they were
closer together than the Abbe limit. This is surely a big difference to
todays super-resolution techniques.

Steffen

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Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy

Mail room:
Marchioninistr. 15, D-81377 München

Building location:
Marchioninistr. 27, München-Großhadern