Live-cell imaging: syringe or peristaltic pump for perfusion?

[G. Esteban Fernandez]

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Hi everyone,

 

We have a POC-R perfusion chamber to image live cultured cells on our Zeiss confocals and are looking to buy a nice pump to push media throught it.  The POC-R user manual says that for physiological conditions we need a flow rate of 0.1 - 0.25 mL/hr (2~4 µL/min).  We've seen syringe and peristaltic pumps that can achieve such rates but aren't entirely sure which mechanism would work best.  What do people here use?  Which might be best to deliver a pulse of drug (short or long) while imaging?

 

Thanks!

 

-Esteban

--
G. Esteban Fernandez, Ph.D.
Associate Director
Molecular Cytology Core Facility
University of Missouri
120 Bond Life Sciences Center
Columbia, MO 65211

http://www.biotech.missouri.edu/mcc/

573-882-4895
573-884-9676 fax

[Ian Harper-2]

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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

the scintilla epicycloidal pumps are the best because the produce an
even flow, ie no peristaltic pressure changes to the cells/cell chamber -
they woudl produce as even a flow as a harvard syringe pump, but no
limits in terms of volume/duration.

can;t remember offhand what the flow specs are, but pls contact
Jim Pringle
Scintilla Scientific Instruments.
Mobile  [phone]
email :  [hidden email]
or
[hidden email]
12 KESWICK GLEN GREENSBOROUGH 3088 VICTORIA AUSTRALIA

[no vested interest]

Ian.

G. Esteban Fernandez wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> Hi everyone,
>  
> We have a POC-R perfusion chamber to image live cultured cells on our
> Zeiss confocals and are looking to buy a nice pump to push media
> throught it.  The POC-R user manual says that for physiological
> conditions we need a flow rate of 0.1 - 0.25 mL/hr (2~4 µL/min).  
> We've seen syringe and peristaltic pumps that can achieve such rates
> but aren't entirely sure which mechanism would work best.  What do
> people here use?  Which might be best to deliver a pulse of drug
> (short or long) while imaging?
>  
> Thanks!
>  
> -Esteban
>
> --
> G. Esteban Fernandez, Ph.D.
> Associate Director
> Molecular Cytology Core Facility
> University of Missouri
> 120 Bond Life Sciences Center
> Columbia, MO 65211
>
> http://www.biotech.missouri.edu/mcc/
>
> 573-882-4895
> 573-884-9676 fax

--

>< >< >< >< >< >< >< >< >< >< >< >< ><
Dr Ian S. Harper, Director
Monash Micro Imaging
School of Biomedical Sciences
Building 13C, Monash University
Clayton, VIC 3800, Australia

Email: [hidden email]
Tel: [phone];  Fax: [phone]
Mobile: [phone]

General enquiries and info:
http://microimaging.monash.org
>< >< >< >< >< >< >< >< >< >< >< >< ><

[B. Prabhakar Pandian]

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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

We have used both peristaltic pumps and syringe pumps and like the
syringe pumps because there is no pulsation in the flow. Plus, you can
reduce the dead volume in the tubing
in the syringe pump drastically in the syringe pumps compared to the
peristaltic. When using small amount of drugs we can have the syringe
head very close to the chamber on a
 micromanipulator and can deliver programmed infusion/withdraw for testing.

-Prabhakar


G. Esteban Fernandez wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> Hi everyone,
>  
> We have a POC-R perfusion chamber to image live cultured cells on our
> Zeiss confocals and are looking to buy a nice pump to push media
> throught it.  The POC-R user manual says that for physiological
> conditions we need a flow rate of 0.1 - 0.25 mL/hr (2~4 µL/min).  
> We've seen syringe and peristaltic pumps that can achieve such rates
> but aren't entirely sure which mechanism would work best.  What do
> people here use?  Which might be best to deliver a pulse of drug
> (short or long) while imaging?
>  
> Thanks!
>  
> -Esteban
>
> --
> G. Esteban Fernandez, Ph.D.
> Associate Director
> Molecular Cytology Core Facility
> University of Missouri
> 120 Bond Life Sciences Center
> Columbia, MO 65211
>
> http://www.biotech.missouri.edu/mcc/
>
> 573-882-4895
> 573-884-9676 fax

--

---------------------------------------------------------
B. Prabhakar Pandian
Principal Scientist (Cellular & Biomolecular Engineering)
Director of Bioengineering Lab Core
CFD Research Corporation
601 Genome Way, Suite 2301
Huntsville, AL 35806
Ph: 256-327-0665
Fax: [phone]

[Andrew Resnick]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

----- Original Message -----
From: "G. Esteban Fernandez" <[hidden email]>
Date: Wednesday, May 21, 2008 7:01 pm
Subject: Live-cell imaging: syringe or peristaltic pump for perfusion?
To: [hidden email]

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi everyone,
>
> We have a POC-R perfusion chamber to image live cultured cells on
> our Zeiss
> confocals and are looking to buy a nice pump to push media throught
> it.  The
> POC-R user manual says that for physiological conditions we need a
> flow rate
> of 0.1 - 0.25 mL/hr (2~4 µL/min).  We've seen syringe and
> peristaltic pumps
> that can achieve such rates but aren't entirely sure which
> mechanism would
> work best.  What do people here use?  Which might be best to
> deliver a pulse
> of drug (short or long) while imaging?
>
> Thanks!
>

I use both- the peristaltic pump is great for chronic flow when the fluid can recirculate.  The
syringe pump is
excellent for extremely slow flow rates and injecting a transient pulse of drug. I have a y-
connector kind of
thing hooked into the tubing that allows a pulse of flud to be injected by the syringe pump
into the peristaltic
line.

I'm curious what your application is- how do you handle fluid mixing, or generic fluid flow
conditions at the
cells? Do you culture in a flow chamber, for example?


Andrew Resnick, Ph.D.
Department of Physiology and Biophysics
Case Western Reserve School of Medicine
Biomedical Research Building, room 820
10900 Euclid Avenue
Cleveland, OH 44106-4948
216-368-6899

>