Live neuronal marker

[Daniel Murphy]

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Hello,

I am looking to track a molecules transport through axons and dendrites in
mouse neurons in culture with a Zeiss LSM 5 LIVE system.  

As far as I know, there is no marker that directly and specifically labels
neurons live, as you can do with MAP2 in immunocytochemistry.  Does anyone
have recommendations for another way to label neurons live or at least to
recognize them?  Ideally we would like to be able to identify and
differentiate between retrograde/anterograde axonal/dendritic trafficking.
Taking BF images gives an OK way of identifying neurons, but since we cannot
do confocal BF stacks, it is difficult to visualize where in Z the BF image are.

Any help would be much appreciated.


Daniel Murphy
Research Technician
Albert Einstein College of Medicine
Optical Imaging Manager, Cell and Molecular Neuroimaging Core
1410 Pelham Parkway South
Bronx, NY 10461
Ph 718-430-4027/8985

[b l]

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Hi Daniel,
Invitrogen sell this baculovirus that expresses MAP4-RFP that is used for neuron imaging in live cell. It is easy to use without cloning.
http://products.invitrogen.com/ivgn/product/C10599?ICID=search-product
best rgdsTerence Tan

--- On Wed, 8/6/11, Daniel Murphy <[hidden email]> wrote:

From: Daniel Murphy <[hidden email]>
Subject: Live neuronal marker
To: [hidden email]
Date: Wednesday, 8 June, 2011, 11:40 PM

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hello,

I am looking to track a molecules transport through axons and dendrites in
mouse neurons in culture with a Zeiss LSM 5 LIVE system. 

As far as I know, there is no marker that directly and specifically labels
neurons live, as you can do with MAP2 in immunocytochemistry.  Does anyone
have recommendations for another way to label neurons live or at least to
recognize them?  Ideally we would like to be able to identify and
differentiate between retrograde/anterograde axonal/dendritic trafficking.
Taking BF images gives an OK way of identifying neurons, but since we cannot
do confocal BF stacks, it is difficult to visualize where in Z the BF image are.

Any help would be much appreciated.


Daniel Murphy
Research Technician
Albert Einstein College of Medicine
Optical Imaging Manager, Cell and Molecular Neuroimaging Core
1410 Pelham Parkway South
Bronx, NY 10461
Ph 718-430-4027/8985

[Francesca Bertani]

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Hi Daniel,
what about using DiI family? Quite used for neuronal and motoneuronal  
tracing, also for long time lapse observations...
Hope it helps
No commercial interest

Francesca


Citando Daniel Murphy <[hidden email]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello,
>
> I am looking to track a molecules transport through axons and dendrites in
> mouse neurons in culture with a Zeiss LSM 5 LIVE system.
>
> As far as I know, there is no marker that directly and specifically labels
> neurons live, as you can do with MAP2 in immunocytochemistry.  Does anyone
> have recommendations for another way to label neurons live or at least to
> recognize them?  Ideally we would like to be able to identify and
> differentiate between retrograde/anterograde axonal/dendritic trafficking.
> Taking BF images gives an OK way of identifying neurons, but since we cannot
> do confocal BF stacks, it is difficult to visualize where in Z the  
> BF image are.
>
> Any help would be much appreciated.
>
>
> Daniel Murphy
> Research Technician
> Albert Einstein College of Medicine
> Optical Imaging Manager, Cell and Molecular Neuroimaging Core
> 1410 Pelham Parkway South
> Bronx, NY 10461
> Ph 718-430-4027/8985
>

Francesca Romana Bertani PhD
ISC-CNR - Dept. Tor Vergata
Via del Fosso del Cavaliere 100
00133 Roma- ITALY
Tel:  [phone]
Fax: [phone]
E-Mail: [hidden email]