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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Any users of Molecular Dynamics MD2001's still out there? Ours just blew its last Ar/Kr laser. My dean says "buy a new confocal", rather than fix this one again (whoo-hoo!). So, while I'm doing the former, anyone care to advise on replacing the gas laser with solid-state lasers? This is only a two-channel instrument, but it gets a lot of undergraduate student use, especially for single-fluor work. I'm guessing we could get by with 473nm and 543nm dpss, but I'm off into unknown territory after that. TIA Julian -- Julian P.S. Smith III Dept. of Biology Winthrop University 520 Cherry Rd. Rock Hill, SC 29733 803-323-2111 x6427 (vox) 803-323-3448 (fax) 803-524-2347 (cell) |
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal You really need to sit down and examine what dyes get used on a regular basis in your facility. Look at the absorption curves of the dyes you regularly use and check whether or not the available solid state lasers will work with them. If they do, then go ahead. With a little research and planning its not that big a deal to swap out the lasers; just make sure they're appropriate for the dyes you are using. Craig On 10/2/07, Julian Smith III <[hidden email]> wrote: > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > Any users of Molecular Dynamics MD2001's still out there? > Ours just blew its last Ar/Kr laser. My dean says "buy a new > confocal", rather than fix this one again (whoo-hoo!). > So, while I'm doing the former, anyone care to advise on replacing > the gas laser with solid-state lasers? This is only a two-channel > instrument, but it gets a lot of undergraduate student use, > especially for single-fluor work. I'm guessing we could get by with > 473nm and 543nm dpss, but I'm off into unknown territory after that. > TIA > Julian > -- > Julian P.S. Smith III > Dept. of Biology > Winthrop University > 520 Cherry Rd. > Rock Hill, SC 29733 > > 803-323-2111 x6427 (vox) > 803-323-3448 (fax) > 803-524-2347 (cell) > |
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In reply to this post by Julian Smith III
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
I would guess that changing
and realigning dichroics and
filters in such a system
could be a hassle, so why not go
with lasers that match your original better
- ie 488nm
and 461nm, both available in DPSS.
(The 473 lasers
are, I think, straight blue diodes, not
DPSS). The small
extra cost of these lasers would probably
be saved in not
having to fiddle with the
dichroics.
Guy
Optical Imaging Techniques in Cell
Biology
by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope Unit, Madsen Building F09, University of Sydney, NSW 2006 ______________________________________________ Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net From: Confocal Microscopy List on behalf of Julian Smith III Sent: Wed 3/10/2007 5:26 AM To: [hidden email] Subject: MD2001 laser conversion? Search the CONFOCAL archive at |
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In reply to this post by Craig Brideau
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G'day List Members, Does anybody have a "Blank" filter block (not the stupid "Open" block) for a Bio-Rad MRC-1024 that they would be prepared to part with? Alternatively one with filters in it would be fine, if you don't need it. I think the Bio-Rad MRC-1000 filter blocks are compatible too (but not a 600 or 500). Thanks Microscopy Manager Central Resource for Advanced Microscopy PO Box 2008 Royal Melbourne Hospital Parkville, Victoria, 3050 Australia Tel: 61 3 9341 3155 Fax: 61 3 9341 3104 email: [hidden email] www.ludwig.edu.au/labs/confocal.html Tip: Learn how to receive reminders about you microscope booking: www.ludwig.edu.au/confocal/Local/Booking_Hint.htm This communication is intended only for the named recipient and may contain information that is confidential, legally privileged or subject to copyright; the Ludwig Institute for Cancer Research does not waiver any rights if you have received this communication in error. The views expressed in this communication are those of the sender and do not necessarily reflect the views of the Ludwig Institute for Cancer Research. |
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