Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalYou really need to sit down and examine what dyes get used on a
regular basis in your facility. Look at the absorption curves of the
dyes you regularly use and check whether or not the available solid
state lasers will work with them. If they do, then go ahead. With a
little research and planning its not that big a deal to swap out the
lasers; just make sure they're appropriate for the dyes you are using.
Craig
On 10/2/07, Julian Smith III <
[hidden email]> wrote:
> Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>
> Any users of Molecular Dynamics MD2001's still out there?
> Ours just blew its last Ar/Kr laser. My dean says "buy a new
> confocal", rather than fix this one again (whoo-hoo!).
> So, while I'm doing the former, anyone care to advise on replacing
> the gas laser with solid-state lasers? This is only a two-channel
> instrument, but it gets a lot of undergraduate student use,
> especially for single-fluor work. I'm guessing we could get by with
> 473nm and 543nm dpss, but I'm off into unknown territory after that.
> TIA
> Julian
> --
> Julian P.S. Smith III
> Dept. of Biology
> Winthrop University
> 520 Cherry Rd.
> Rock Hill, SC 29733
>
> 803-323-2111 x6427 (vox)
> 803-323-3448 (fax)
> 803-524-2347 (cell)
>
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