Maintenance costs of multi photon systems

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Daniel Fimiarz Daniel Fimiarz
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Maintenance costs of multi photon systems

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hello,

I am currently researching a possibility of acquiring a multi photon
system for our imaging core facility. What are typical maintenance
costs of such systems? Are they very different from single photon
(confocal) systems?  I do realize that my question is rather general
and I am not expecting very detailed answers. Anything that you could
share would be helpful.

Thank you very much,

Daniel F.
Armstrong, Brian Armstrong, Brian
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Re: Maintenance costs of multi photon systems

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

For a general answer to your general question, I would say that the
difference is the laser. A service contract is roughly 10% of the cost
of the equipment so a service contract on a 2P laser will be more than a
vis laser. A 2P laser is around $200k and the contract is around $15k
yearly.
Also, given that a 2P system would lack a scan head it may be more
simple and less expensive to maintain. However, you may consider a
service contract here as well which would add expense after the first
year following normal warranty coverage. If you want to talk more about
2P systems you can contact me directly. Cheers,

Brian D Armstrong PhD
Light Microscopy Core Manager
Beckman Research Institute
City of Hope
1450 E Duarte Rd
Duarte, CA 91010
626-359-8111 x62872
http://www.cityofhope.org/SharedResources/LightMicroscopy

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Daniel Fimiarz
Sent: Tuesday, October 09, 2007 7:06 AM
To: [hidden email]
Subject: Maintenance costs of multi photon systems

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hello,

I am currently researching a possibility of acquiring a multi photon
system for our imaging core facility. What are typical maintenance
costs of such systems? Are they very different from single photon
(confocal) systems?  I do realize that my question is rather general
and I am not expecting very detailed answers. Anything that you could
share would be helpful.

Thank you very much,

Daniel F.


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Craig Brideau Craig Brideau
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Re: Maintenance costs of multi photon systems

In reply to this post by Daniel Fimiarz
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

In terms of the laser it self, there are a few things to consider.  If
you go with a Ti:Saph laser, it doesn't actually need a whole lot from
day to day.  From my experience with our own lab's laser, it needs an
occasional top-up of distilled/filtered water for the cooling chiller
(maybe a liter once every couple months!).  If you plan on running the
laser below 860nm or so you generally have to purge the laser cavity
with ultra pure (5.0) dry nitrogen.  Our own lab has a researcher that
works at 925nm and we go through a tank every couple months.  Finally,
most Ti:Saphs use diode lasers as a pump source.  These diodes can
work for anywhere from 5 to 10 years, and cost about 10-20k to
replace.
From a facilities standpoint, Ti:Saphs require a fairly clean,
temperature-stable environment.  They put out a lot of heat from the
chiller, so it is important to ensure your room has adequate air
conditioning to handle the thermal load.  If the temperature changes
more than a couple degrees over the course of a day it can be bad for
the laser.

Hope this helps!

Craig

On 10/9/07, Daniel Fimiarz <[hidden email]> wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hello,
>
> I am currently researching a possibility of acquiring a multi photon
> system for our imaging core facility. What are typical maintenance
> costs of such systems? Are they very different from single photon
> (confocal) systems?  I do realize that my question is rather general
> and I am not expecting very detailed answers. Anything that you could
> share would be helpful.
>
> Thank you very much,
>
> Daniel F.
>
Mark Cannell Mark Cannell
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Re: Maintenance costs of multi photon systems

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Craig

Why use expensive ultra pure nitrogen? I thought it was mostly water  
vapor that you need to reduce in the cavity so that regular grade  
nitrogen should be OK. (the H2O absorbance reduces the cavity gain)  
Come to that, even dried air might be suitable.

Cheers Mark

On 10/10/2007, at 6:13 AM, Craig Brideau wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> In terms of the laser it self, there are a few things to consider.  If
> you go with a Ti:Saph laser, it doesn't actually need a whole lot from
> day to day.  From my experience with our own lab's laser, it needs an
> occasional top-up of distilled/filtered water for the cooling chiller
> (maybe a liter once every couple months!).  If you plan on running the
> laser below 860nm or so you generally have to purge the laser cavity
> with ultra pure (5.0) dry nitrogen.  Our own lab has a researcher that
> works at 925nm and we go through a tank every couple months.  Finally,
> most Ti:Saphs use diode lasers as a pump source.  These diodes can
> work for anywhere from 5 to 10 years, and cost about 10-20k to
> replace.
> From a facilities standpoint, Ti:Saphs require a fairly clean,
> temperature-stable environment.  They put out a lot of heat from the
> chiller, so it is important to ensure your room has adequate air
> conditioning to handle the thermal load.  If the temperature changes
> more than a couple degrees over the course of a day it can be bad for
> the laser.
>
> Hope this helps!
>
> Craig
>
> On 10/9/07, Daniel Fimiarz <[hidden email]> wrote:
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Hello,
>>
>> I am currently researching a possibility of acquiring a multi photon
>> system for our imaging core facility. What are typical maintenance
>> costs of such systems? Are they very different from single photon
>> (confocal) systems?  I do realize that my question is rather general
>> and I am not expecting very detailed answers. Anything that you could
>> share would be helpful.
>>
>> Thank you very much,
>>
>> Daniel F.
>>
Karl Garsha Karl Garsha
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Re: Maintenance costs of multi photon systems

In reply to this post by Daniel Fimiarz
Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hello Daniel,

You should consider the maintenance/expendables associated with the pump laser; the diodes will likely need to be replaced after a certain number of hours, this may be a cost you want to plan ahead for.
MAG Signature

 

Karl Garsha
Head Application Scientist
Microimaging Applications Group (MAG)
3440
East Britannia Drive
Tucson, AZ 85629

tel 520.889.9933
direct 520.547.2704
fax 520.573.1944


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Daniel Fimiarz wrote:
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hello,

I am currently researching a possibility of acquiring a multi photon
system for our imaging core facility. What are typical maintenance
costs of such systems? Are they very different from single photon
(confocal) systems?  I do realize that my question is rather general
and I am not expecting very detailed answers. Anything that you could
share would be helpful.

Thank you very much,

Daniel F.
  
John Hyun-2 John Hyun-2
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Re: EM Job Openings

In reply to this post by Mark Cannell
Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Re: EM Job Openings Please see job openings for EM application professionals for Gatan, Inc below. Thank you.


EELS Application Specialist


Job responsibility:
Support Gatan’s highly successful Analytical TEM product line (GIF and EELS spectrometers).
Typical responsibilities include working with key users to develop new EELS/GIF applications, providing customer training and support, conducting product demonstrations, representing Gatan at scientific conferences, and assisting new product development.
 
Certain amount of travel is required.
 
Qualification:
  • Strong EELS background.  
  • Hands-on experience in EELS, EDS, STEM, and EFTEM.
  • Prior experience in Gatan products (GIF or EELS spectrometers)
  • A Ph.D. degree in physics, chemistry, materials science or engineering.
  • Publications of analytical TEM work (EELS or EFTEM)
  • Excellent verbal, written, and interpersonal communication skills in English

 
 
The position is based in Pleasanton, California and carries a salary commensurate with experience and an attractive benefits package.
 
Please submit your resume to [hidden email].  


Marketing Product Manager - Analytical TEM Products


Job responsibility:
Develop business strategies and product line roadmap, acquire direct input from customers on new products, provide technical support for customers and sales, conduct workshops and seminars.  Responsible for product line P&L.
 
Certain amount of travel is required.
 
Qualification:
  • Previous commercial experience in product management for a scientific instrument company (electron microscopy industry) is preferred but not necessary
  • Knowledge and experience in TEM/STEM, EELS, EDS, and EFTEM.  
  • Previous experience in Gatan products (GIF or EELS spectrometers) preferred
  • A Ph.D. degree in physics, chemistry, materials science or engineering.
  • Excellent verbal, written, and interpersonal communication skills in English

 
The position is based in Pleasanton, California and carries a salary commensurate with experience and an attractive benefits package.
 
Please submit your resume to [hidden email]



3View Application Specialist


3View is Gatan’s latest product aimed at acquiring 3D image data in a SEM from life science specimens over significant volumes with large field of view and high resolution. 3View is based on the ground breaking technique developed by MPI Heidelberg (W Denk and H Horstmann, Max-Planck Institute for Medical Research, Heidelberg, Germany. “Serial block-face scanning electron microscopy to reconstruct three-dimensional tissue nanostructure”. Plos Biology, 2004.2(11):p. 1900-1909), with special application to the neuroscience community
 
Job responsibility:
Provide technical support of 3View product.  Responsibilities include product demonstrations, presentations, working with customers to acquire practical data, generation of sales and marketing literature, and definition of new product features.
Frequent worldwide travel may be required.
 
Qualification:
  • Hands-on experience in sample preparations for ultramicrotomy (fixation and embedment, and subsequent generation of ultra-thin sections
  • Hands-on experience of SEM/TEM and/or light microscopy (LM)  
  • An advanced degree in related life science field is preferred
  • Experience using imaging software including 3D image reconstruction
  • Excellent verbal, written, and interpersonal communication skills in English

 
The position is based in Pleasanton, California and carries a salary commensurate with experience and an attractive benefits package.
 
Please submit your resume to [hidden email] [hidden email]  and mention 3View Application Specialist.  
Craig Brideau Craig Brideau
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Re: Maintenance costs of multi photon systems

In reply to this post by Mark Cannell
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Two words: Dust Particles.
Any dirt that makes it into the optical cavity will shorten the life
of the laser.  The reason to use the pure nitrogen is that its very,
very clean.  When you are dealing with the kind of power levels that
get slung around inside the optical cavity of a Ti:Saph, you don't
want any contamination!  If you can get super clean dry air then more
power to you, but why risk a $100k laser for the sake of a few bucks
of pure n2?

Craig


On 10/10/07, Mark Cannell <[hidden email]> wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi Craig
>
> Why use expensive ultra pure nitrogen? I thought it was mostly water
> vapor that you need to reduce in the cavity so that regular grade
> nitrogen should be OK. (the H2O absorbance reduces the cavity gain)
> Come to that, even dried air might be suitable.
>
> Cheers Mark
>
> On 10/10/2007, at 6:13 AM, Craig Brideau wrote:
>
> > Search the CONFOCAL archive at
> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> > In terms of the laser it self, there are a few things to consider.  If
> > you go with a Ti:Saph laser, it doesn't actually need a whole lot from
> > day to day.  From my experience with our own lab's laser, it needs an
> > occasional top-up of distilled/filtered water for the cooling chiller
> > (maybe a liter once every couple months!).  If you plan on running the
> > laser below 860nm or so you generally have to purge the laser cavity
> > with ultra pure (5.0) dry nitrogen.  Our own lab has a researcher that
> > works at 925nm and we go through a tank every couple months.  Finally,
> > most Ti:Saphs use diode lasers as a pump source.  These diodes can
> > work for anywhere from 5 to 10 years, and cost about 10-20k to
> > replace.
> > From a facilities standpoint, Ti:Saphs require a fairly clean,
> > temperature-stable environment.  They put out a lot of heat from the
> > chiller, so it is important to ensure your room has adequate air
> > conditioning to handle the thermal load.  If the temperature changes
> > more than a couple degrees over the course of a day it can be bad for
> > the laser.
> >
> > Hope this helps!
> >
> > Craig
> >
> > On 10/9/07, Daniel Fimiarz <[hidden email]> wrote:
> >> Search the CONFOCAL archive at
> >> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >>
> >> Hello,
> >>
> >> I am currently researching a possibility of acquiring a multi photon
> >> system for our imaging core facility. What are typical maintenance
> >> costs of such systems? Are they very different from single photon
> >> (confocal) systems?  I do realize that my question is rather general
> >> and I am not expecting very detailed answers. Anything that you could
> >> share would be helpful.
> >>
> >> Thank you very much,
> >>
> >> Daniel F.
> >>
>
Mark Cannell Mark Cannell
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Re: Maintenance costs of multi photon systems

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Craig

Interesting idea, but in 10 years of operating a Ti:S, I've not  
noticed any problem with dust flying around inside the cavity. (You  
can see room dust when working on the cavity with the covers off). In  
any case, as far as I know, commercial compressed gasses _are_  
filtered. Here's an alternative: N2 blow off from a liquid N2 dewar -
it is dry and squeeky clean...

Cheers Mark


On 11/10/2007, at 5:25 PM, Craig Brideau wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Two words: Dust Particles.
> Any dirt that makes it into the optical cavity will shorten the life
> of the laser.  The reason to use the pure nitrogen is that its very,
> very clean.  When you are dealing with the kind of power levels that
> get slung around inside the optical cavity of a Ti:Saph, you don't
> want any contamination!  If you can get super clean dry air then more
> power to you, but why risk a $100k laser for the sake of a few bucks
> of pure n2?
>
> Craig
>
>
> On 10/10/07, Mark Cannell <[hidden email]> wrote:
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Hi Craig
>>
>> Why use expensive ultra pure nitrogen? I thought it was mostly water
>> vapor that you need to reduce in the cavity so that regular grade
>> nitrogen should be OK. (the H2O absorbance reduces the cavity gain)
>> Come to that, even dried air might be suitable.
>>
>> Cheers Mark
>>
>> On 10/10/2007, at 6:13 AM, Craig Brideau wrote:
>>
>>> Search the CONFOCAL archive at
>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>>
>>> In terms of the laser it self, there are a few things to  
>>> consider.  If
>>> you go with a Ti:Saph laser, it doesn't actually need a whole lot  
>>> from
>>> day to day.  From my experience with our own lab's laser, it  
>>> needs an
>>> occasional top-up of distilled/filtered water for the cooling  
>>> chiller
>>> (maybe a liter once every couple months!).  If you plan on  
>>> running the
>>> laser below 860nm or so you generally have to purge the laser cavity
>>> with ultra pure (5.0) dry nitrogen.  Our own lab has a researcher  
>>> that
>>> works at 925nm and we go through a tank every couple months.  
>>> Finally,
>>> most Ti:Saphs use diode lasers as a pump source.  These diodes can
>>> work for anywhere from 5 to 10 years, and cost about 10-20k to
>>> replace.
>>> From a facilities standpoint, Ti:Saphs require a fairly clean,
>>> temperature-stable environment.  They put out a lot of heat from the
>>> chiller, so it is important to ensure your room has adequate air
>>> conditioning to handle the thermal load.  If the temperature changes
>>> more than a couple degrees over the course of a day it can be bad  
>>> for
>>> the laser.
>>>
>>> Hope this helps!
>>>
>>> Craig
>>>
>>> On 10/9/07, Daniel Fimiarz <[hidden email]> wrote:
>>>> Search the CONFOCAL archive at
>>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>>>
>>>> Hello,
>>>>
>>>> I am currently researching a possibility of acquiring a multi  
>>>> photon
>>>> system for our imaging core facility. What are typical maintenance
>>>> costs of such systems? Are they very different from single photon
>>>> (confocal) systems?  I do realize that my question is rather  
>>>> general
>>>> and I am not expecting very detailed answers. Anything that you  
>>>> could
>>>> share would be helpful.
>>>>
>>>> Thank you very much,
>>>>
>>>> Daniel F.
>>>>
>>
Craig Brideau Craig Brideau
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Re: Maintenance costs of multi photon systems

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Our lab is very dusty due to an old ventilation system, so our problem
may be worse than yours.  As with any lab and laser system, "your
mileage may vary" depending on your specific condtions.  The N2 dewar
idea is very interesting.  What do you use in your system?

Craig


On 10/10/07, Mark Cannell <[hidden email]> wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi Craig
>
> Interesting idea, but in 10 years of operating a Ti:S, I've not
> noticed any problem with dust flying around inside the cavity. (You
> can see room dust when working on the cavity with the covers off). In
> any case, as far as I know, commercial compressed gasses _are_
> filtered. Here's an alternative: N2 blow off from a liquid N2 dewar -
> it is dry and squeeky clean...
>
> Cheers Mark
>
>
> On 11/10/2007, at 5:25 PM, Craig Brideau wrote:
>
> > Search the CONFOCAL archive at
> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> > Two words: Dust Particles.
> > Any dirt that makes it into the optical cavity will shorten the life
> > of the laser.  The reason to use the pure nitrogen is that its very,
> > very clean.  When you are dealing with the kind of power levels that
> > get slung around inside the optical cavity of a Ti:Saph, you don't
> > want any contamination!  If you can get super clean dry air then more
> > power to you, but why risk a $100k laser for the sake of a few bucks
> > of pure n2?
> >
> > Craig
> >
> >
> > On 10/10/07, Mark Cannell <[hidden email]> wrote:
> >> Search the CONFOCAL archive at
> >> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >>
> >> Hi Craig
> >>
> >> Why use expensive ultra pure nitrogen? I thought it was mostly water
> >> vapor that you need to reduce in the cavity so that regular grade
> >> nitrogen should be OK. (the H2O absorbance reduces the cavity gain)
> >> Come to that, even dried air might be suitable.
> >>
> >> Cheers Mark
> >>
> >> On 10/10/2007, at 6:13 AM, Craig Brideau wrote:
> >>
> >>> Search the CONFOCAL archive at
> >>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >>>
> >>> In terms of the laser it self, there are a few things to
> >>> consider.  If
> >>> you go with a Ti:Saph laser, it doesn't actually need a whole lot
> >>> from
> >>> day to day.  From my experience with our own lab's laser, it
> >>> needs an
> >>> occasional top-up of distilled/filtered water for the cooling
> >>> chiller
> >>> (maybe a liter once every couple months!).  If you plan on
> >>> running the
> >>> laser below 860nm or so you generally have to purge the laser cavity
> >>> with ultra pure (5.0) dry nitrogen.  Our own lab has a researcher
> >>> that
> >>> works at 925nm and we go through a tank every couple months.
> >>> Finally,
> >>> most Ti:Saphs use diode lasers as a pump source.  These diodes can
> >>> work for anywhere from 5 to 10 years, and cost about 10-20k to
> >>> replace.
> >>> From a facilities standpoint, Ti:Saphs require a fairly clean,
> >>> temperature-stable environment.  They put out a lot of heat from the
> >>> chiller, so it is important to ensure your room has adequate air
> >>> conditioning to handle the thermal load.  If the temperature changes
> >>> more than a couple degrees over the course of a day it can be bad
> >>> for
> >>> the laser.
> >>>
> >>> Hope this helps!
> >>>
> >>> Craig
> >>>
> >>> On 10/9/07, Daniel Fimiarz <[hidden email]> wrote:
> >>>> Search the CONFOCAL archive at
> >>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >>>>
> >>>> Hello,
> >>>>
> >>>> I am currently researching a possibility of acquiring a multi
> >>>> photon
> >>>> system for our imaging core facility. What are typical maintenance
> >>>> costs of such systems? Are they very different from single photon
> >>>> (confocal) systems?  I do realize that my question is rather
> >>>> general
> >>>> and I am not expecting very detailed answers. Anything that you
> >>>> could
> >>>> share would be helpful.
> >>>>
> >>>> Thank you very much,
> >>>>
> >>>> Daniel F.
> >>>>
> >>
>
Mark Cannell Mark Cannell
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Re: Maintenance costs of multi photon systems

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi

We've only used junk grade N2 at 900 nm -but then we don't go there  
generally.

Cheers

On 11/10/2007, at 6:05 PM, Craig Brideau wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Our lab is very dusty due to an old ventilation system, so our problem
> may be worse than yours.  As with any lab and laser system, "your
> mileage may vary" depending on your specific condtions.  The N2 dewar
> idea is very interesting.  What do you use in your system?
>
> Craig
>
>
> On 10/10/07, Mark Cannell <[hidden email]> wrote:
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Hi Craig
>>
>> Interesting idea, but in 10 years of operating a Ti:S, I've not
>> noticed any problem with dust flying around inside the cavity. (You
>> can see room dust when working on the cavity with the covers off). In
>> any case, as far as I know, commercial compressed gasses _are_
>> filtered. Here's an alternative: N2 blow off from a liquid N2 dewar -
>> it is dry and squeeky clean...
>>
>> Cheers Mark
>>
>>
>> On 11/10/2007, at 5:25 PM, Craig Brideau wrote:
>>
>>> Search the CONFOCAL archive at
>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>>
>>> Two words: Dust Particles.
>>> Any dirt that makes it into the optical cavity will shorten the life
>>> of the laser.  The reason to use the pure nitrogen is that its very,
>>> very clean.  When you are dealing with the kind of power levels that
>>> get slung around inside the optical cavity of a Ti:Saph, you don't
>>> want any contamination!  If you can get super clean dry air then  
>>> more
>>> power to you, but why risk a $100k laser for the sake of a few bucks
>>> of pure n2?
>>>
>>> Craig
>>>
>>>
>>> On 10/10/07, Mark Cannell <[hidden email]> wrote:
>>>> Search the CONFOCAL archive at
>>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>>>
>>>> Hi Craig
>>>>
>>>> Why use expensive ultra pure nitrogen? I thought it was mostly  
>>>> water
>>>> vapor that you need to reduce in the cavity so that regular grade
>>>> nitrogen should be OK. (the H2O absorbance reduces the cavity gain)
>>>> Come to that, even dried air might be suitable.
>>>>
>>>> Cheers Mark
>>>>
>>>> On 10/10/2007, at 6:13 AM, Craig Brideau wrote:
>>>>
>>>>> Search the CONFOCAL archive at
>>>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>>>>
>>>>> In terms of the laser it self, there are a few things to
>>>>> consider.  If
>>>>> you go with a Ti:Saph laser, it doesn't actually need a whole lot
>>>>> from
>>>>> day to day.  From my experience with our own lab's laser, it
>>>>> needs an
>>>>> occasional top-up of distilled/filtered water for the cooling
>>>>> chiller
>>>>> (maybe a liter once every couple months!).  If you plan on
>>>>> running the
>>>>> laser below 860nm or so you generally have to purge the laser  
>>>>> cavity
>>>>> with ultra pure (5.0) dry nitrogen.  Our own lab has a researcher
>>>>> that
>>>>> works at 925nm and we go through a tank every couple months.
>>>>> Finally,
>>>>> most Ti:Saphs use diode lasers as a pump source.  These diodes can
>>>>> work for anywhere from 5 to 10 years, and cost about 10-20k to
>>>>> replace.
>>>>> From a facilities standpoint, Ti:Saphs require a fairly clean,
>>>>> temperature-stable environment.  They put out a lot of heat  
>>>>> from the
>>>>> chiller, so it is important to ensure your room has adequate air
>>>>> conditioning to handle the thermal load.  If the temperature  
>>>>> changes
>>>>> more than a couple degrees over the course of a day it can be bad
>>>>> for
>>>>> the laser.
>>>>>
>>>>> Hope this helps!
>>>>>
>>>>> Craig
>>>>>
>>>>> On 10/9/07, Daniel Fimiarz <[hidden email]> wrote:
>>>>>> Search the CONFOCAL archive at
>>>>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>>>>>
>>>>>> Hello,
>>>>>>
>>>>>> I am currently researching a possibility of acquiring a multi
>>>>>> photon
>>>>>> system for our imaging core facility. What are typical  
>>>>>> maintenance
>>>>>> costs of such systems? Are they very different from single photon
>>>>>> (confocal) systems?  I do realize that my question is rather
>>>>>> general
>>>>>> and I am not expecting very detailed answers. Anything that you
>>>>>> could
>>>>>> share would be helpful.
>>>>>>
>>>>>> Thank you very much,
>>>>>>
>>>>>> Daniel F.
>>>>>>
>>>>
>>
Ron Anderson-4 Ron Anderson-4
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Re: EM Job Openings

In reply to this post by John Hyun-2
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

John,

I noticed with interest your recent employment note on the Microscopy
listserver and hope that you are having good success.

Our publication, Microscopy Today, is mailed six times per year to over
16,000 microscopists -- each of whom has either specifically requested
the publication or were automatically subscribed as an MSA member
benefit. Our next issue closes soon and will be mailed on or about
January 15th. You may consider placing your employment ad with us.

We offer employment advertisements at the reduced rate of $475 for a
quarter-page (3 5/8 in. W X 5 in H) insertion. Larger (3 5/8" W)
employment ads can be purchased for an additional $90 per inch over 5
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received, we will publish. You may include a logo if you wish.

Kindly advise rather soon should you be interested in this proposal as
the timing for this issue is becoming critical.

Regards,

Ron Anderson, Editor
Microscopy Today

John Hyun wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Please see job
> openings for EM application professionals for Gatan, Inc below. Thank you.
>
>
>     EELS Application Specialist
>
>
> Job responsibility:
> Support Gatan’s highly successful Analytical TEM product line (GIF and
> EELS spectrometers).
> Typical responsibilities include working with key users to develop new
> EELS/GIF applications, providing customer training and support,
> conducting product demonstrations, representing Gatan at scientific
> conferences, and assisting new product development.
>
> Certain amount of travel is required.
>
> Qualification:
>
>     * Strong EELS background.
>     * Hands-on experience in EELS, EDS, STEM, and EFTEM.
>     * Prior experience in Gatan products (GIF or EELS spectrometers)
>     * A Ph.D. degree in physics, chemistry, materials science or
>       engineering.
>     * Publications of analytical TEM work (EELS or EFTEM)
>     * Excellent verbal, written, and interpersonal communication
>       skills in English
>
>
>
>
> The position is based in Pleasanton, California and carries a salary
> commensurate with experience and an attractive benefits package.
>
> Please submit your resume to [hidden email].
>
>
>     Marketing Product Manager - Analytical TEM Products
>
>
> Job responsibility:
> Develop business strategies and product line roadmap, acquire direct
> input from customers on new products, provide technical support for
> customers and sales, conduct workshops and seminars. Responsible for
> product line P&L.
>
> Certain amount of travel is required.
>
> Qualification:
>
>     * Previous commercial experience in product management for a
>       scientific instrument company (electron microscopy industry) is
>       preferred but not necessary
>     * Knowledge and experience in TEM/STEM, EELS, EDS, and EFTEM.
>     * Previous experience in Gatan products (GIF or EELS
>       spectrometers) preferred
>     * A Ph.D. degree in physics, chemistry, materials science or
>       engineering.
>     * Excellent verbal, written, and interpersonal communication
>       skills in English
>
>
>
> The position is based in Pleasanton, California and carries a salary
> commensurate with experience and an attractive benefits package.
>
> Please submit your resume to [hidden email]
>
>
>
>     3View� Application Specialist
>
>
> 3View is Gatan’s latest product aimed at acquiring 3D image data in a
> SEM from life science specimens over significant volumes with large
> field of view and high resolution. 3View is based on the ground
> breaking technique developed by MPI Heidelberg (W Denk and H
> Horstmann, Max-Planck Institute for Medical Research, Heidelberg,
> Germany. /“Serial block-face scanning electron microscopy to
> reconstruct three-dimensional tissue nanostructure”/. Plos Biology,
> 2004.2(11):p. 1900-1909), with special application to the neuroscience
> community
>
> Job responsibility:
> Provide technical support of 3View product. Responsibilities include
> product demonstrations, presentations, working with customers to
> acquire practical data, generation of sales and marketing literature,
> and definition of new product features.
> Frequent worldwide travel may be required.
>
> Qualification:
>
>     * Hands-on experience in sample preparations for ultramicrotomy
>       (fixation and embedment, and subsequent generation of ultra-thin
>       sections
>     * Hands-on experience of SEM/TEM and/or light microscopy (LM)
>     * An advanced degree in related life science field is preferred
>     * Experience using imaging software including 3D image reconstruction
>     * Excellent verbal, written, and interpersonal communication
>       skills in English
>
>
>
> The position is based in Pleasanton, California and carries a salary
> commensurate with experience and an attractive benefits package.
>
> Please submit your resume to [hidden email]
> <mailto:[hidden email]> and mention 3View Application
> Specialist.