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Mattek dishes

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Jennes, Mary E

Mattek dishes

Has anyone ever reused Mattek dishes? I have a user who is trying to save some money and is cleaning their dishes with a detergent, rinsing and exposing to UV light. The cells look “funny’ to me in that I can’t seem to get a crisp image. Does anyone have any experience with reusing dishes?

Thx!

Mary

Alice Rodriguez Diaz

Re: Mattek dishes

Hi,

Are they coated? I probably wouldn’t reuse them but if did, I’d make sure I got the coating right… poly-lysine or other.

Best,

Alice Rodriguez-Diaz

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Jennes, Mary E
Sent: Thursday, November 05, 2009 11:46 AM
To: [hidden email]
Subject: Mattek dishes

Thx!

Mary

sgkcck

Re: Mattek dishes

In reply to this post by Jennes, Mary E

No they are not

Thank you and we look forward to hearing from you

Sincerely

Stacie Kirsch
Electron Microscopy Sciences
1560 Industry Road
Hatfield Pa 19440
Tel: 215-412-8400 Fax: 215-412-8450
E-Mail: [hidden email] or [hidden email]
Web: www.emsdiasum.com

In a message dated 11/5/2009 1:07:32 P.M. Eastern Standard Time, arodrig2722@... writes:

Hi,

Are they coated? I probably wouldn’t reuse them but if did, I’d make sure I got the coating right… poly-lysine or other.

Best,

Alice Rodriguez-Diaz

From: Confocal Microscopy List [mailto:CONFOCALMICROSCOPY@...] On Behalf Of Jennes, Mary E
Sent: Thursday, November 05, 2009 11:46 AM
To: CONFOCALMICROSCOPY@...
Subject: Mattek dishes

Has anyone ever reused Mattek dishes? I have a user who is trying to save some money and is cleaning their dishes with a detergent, rinsing and exposing to UV light. The cells look “funny’ to me in that I can’t seem to get a crisp image. Does anyone have any experience with reusing dishes?

Thx!

Mary

Jennes, Mary E

Re: Mattek dishes

In reply to this post by Alice Rodriguez Diaz

They are purchased non-coated then etched in 50% Nitric acid overnight, Cleaned With Micro (detergent), series of EToH and Water rinses, then 30’ UV exposure.

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Alice Rodriguez Diaz
Sent: Thursday, November 05, 2009 1:07 PM
To: [hidden email]
Subject: Re: Mattek dishes

Hi,

Are they coated? I probably wouldn’t reuse them but if did, I’d make sure I got the coating right… poly-lysine or other.

Best,

Alice Rodriguez-Diaz

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Jennes, Mary E
Sent: Thursday, November 05, 2009 11:46 AM
To: [hidden email]
Subject: Mattek dishes

Thx!

Mary

Tim Feinstein-2

Re: Mattek dishes

<base href="x-msg://4/">Acid washing them before each use may help. It is possible that you detergent does not clear all adherent protein and extracellular matrix off the glass surface.

cheers,

Tim Feinstein

On Nov 5, 2009, at 1:26 PM, Jennes, Mary E wrote:

They are purchased non-coated then etched in 50% Nitric acid overnight, Cleaned With Micro (detergent), series of EToH and Water rinses, then 30’ UV exposure.

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Alice Rodriguez Diaz
Sent: Thursday, November 05, 2009 1:07 PM
To: [hidden email]
Subject: Re: Mattek dishes

Hi,

Are they coated? I probably wouldn’t reuse them but if did, I’d make sure I got the coating right… poly-lysine or other.

Best,

Alice Rodriguez-Diaz

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Jennes, Mary E
Sent: Thursday, November 05, 2009 11:46 AM
To: [hidden email]
Subject: Mattek dishes

Has anyone ever reused Mattek dishes? I have a user who is trying to save some money and is cleaning their dishes with a detergent, rinsing and exposing to UV light. The cells look “funny’ to me in that I can’t seem to get a crisp image. Does anyone have any experience with reusing dishes?

Thx!

Mary

Alice Rodriguez Diaz

Re: Mattek dishes

In reply to this post by Jennes, Mary E

Hi,

I believe it depends on what cells you are using, but they may just not tolerate the washing procedure… any type of residue will have an effect on adherence… this is one place where I wouldn’t try to cut corners…

Best,

Alice

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Jennes, Mary E
Sent: Thursday, November 05, 2009 12:27 PM
To: [hidden email]
Subject: Re: Mattek dishes

They are purchased non-coated then etched in 50% Nitric acid overnight, Cleaned With Micro (detergent), series of EToH and Water rinses, then 30’ UV exposure.

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Alice Rodriguez Diaz
Sent: Thursday, November 05, 2009 1:07 PM
To: [hidden email]
Subject: Re: Mattek dishes

Hi,

Are they coated? I probably wouldn’t reuse them but if did, I’d make sure I got the coating right… poly-lysine or other.

Best,

Alice Rodriguez-Diaz

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Jennes, Mary E
Sent: Thursday, November 05, 2009 11:46 AM
To: [hidden email]
Subject: Mattek dishes

Thx!

Mary

S. Brunet

Re: Mattek dishes

Hello,

I would look at the dishes using transmitted light or DIC after the cleaning
procedure. I would also verify whether the glue can handle the solvents used.

In general, I would not recommend reusing the dishes. It may not save money.
It takes time and chemicals to clean them and you have results that are not
satisfactory.

Good luck!
Sophie Brunet
____________________________________________________
Sophie M. K. Brunet, Ph. D.
Research Officer
Optical Spectroscopy, Laser Systems and Applications
[hidden email]
306-966-1719 (office) 306-966-1702 (fax)
____________________________________________________
Saskatchewan Structural Sciences Centre
University of Saskatchewan
Thorvaldson Bldg.
110 Science Place
Saskatoon, Sk S7N 5C9
____________________________________________________

Quoting Alice Rodriguez Diaz <[hidden email]>:

> Hi,
>
>
>
> I believe it depends on what cells you are using, but they may just not
> tolerate the washing procedure. any type of residue will have an effect on
> adherence. this is one place where I wouldn't try to cut corners.
>
>
>
> Best,
>
>
>
> Alice
>
> From: Confocal Microscopy List [mailto:[hidden email]] On
> Behalf Of Jennes, Mary E
> Sent: Thursday, November 05, 2009 12:27 PM
> To: [hidden email]
> Subject: Re: Mattek dishes
>
>
>
> They are purchased non-coated then etched in 50% Nitric acid overnight,
> Cleaned With Micro (detergent), series of EToH and Water rinses, then 30' UV
> exposure.
>
>
>
> _____
>
> From: Confocal Microscopy List [mailto:[hidden email]] On
> Behalf Of Alice Rodriguez Diaz
> Sent: Thursday, November 05, 2009 1:07 PM
> To: [hidden email]
> Subject: Re: Mattek dishes
>
>
>
> Hi,
>
>
>
> Are they coated? I probably wouldn't reuse them but if did, I'd make sure I
> got the coating right. poly-lysine or other.
>
>
>
> Best,
>
>
>
> Alice Rodriguez-Diaz
>
>
>
>
>
> From: Confocal Microscopy List [mailto:[hidden email]] On
> Behalf Of Jennes, Mary E
> Sent: Thursday, November 05, 2009 11:46 AM
> To: [hidden email]
> Subject: Mattek dishes
>
>
>
> Has anyone ever reused Mattek dishes? I have a user who is trying to save
> some money and is cleaning their dishes with a detergent, rinsing and
> exposing to UV light. The cells look "funny' to me in that I can't seem to
> get a crisp image. Does anyone have any experience with reusing dishes?
>
>
>
> Thx!
>
>
>
> Mary
>
>

Rossi, Ann [BSD] - MED

Re: Mattek dishes

In reply to this post by Jennes, Mary E

RE: Mattek dishes

I've reused uncoated Mattek dishes for primary skeletal muscle fibers used the same day as they were isolated. I found that a quick sponge wash with Alconox followed by a rinse in distilled tap water and then a 70% ethanol rinse worked quite well. But, I only could wash the dishes twice before subsequent fiber preps would stop adhering to the glass. Never had a problem with the "crispness" of images, as long as the cells stuck to the dish.

Ann E. Rossi, Ph.D.
Postdoctoral Fellow
University of Chicago
Department of Medicine

-----Original Message-----
From: Confocal Microscopy List on behalf of Jennes, Mary E
Sent: Thu 11/5/2009 11:46 AM
To: [hidden email]
Subject: Mattek dishes

Has anyone ever reused Mattek dishes? I have a user who is trying to save some money and is cleaning their dishes with a detergent, rinsing and exposing to UV light. The cells look "funny' to me in that I can't seem to get a crisp image. Does anyone have any experience with reusing dishes?

Thx!

Mary

This email is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this email message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is prohibited. If you have received this email in error, please notify the sender and destroy/delete all copies of the transmittal. Thank you.

Vladimir Ghukasyan-2

Re: Mattek dishes

In reply to this post by Jennes, Mary E

Instead of Mattek dishes we're just using a system of metallic rings
and 24mm round coverslips. This is much cheaper solution. Let me know
if you're interested, I'll send you a photo of the rings we have.

With regards,
Vladimir

-----------------------------------------
Vladimir Ghukasyan, Ph.D.
Institute of Biophotonics
National Yang-Ming University
155, Li-Nong St., Sec. 2
Taipei 112, Taiwan
Tel.: (886 2) 28267000 ext. 5709

On Fri, Nov 6, 2009 at 1:46 AM, Jennes, Mary E <[hidden email]> wrote:

> Has anyone ever reused Mattek dishes? I have a user who is trying to save
> some money and is cleaning their dishes with a detergent, rinsing and
> exposing to UV light. The cells look “funny’ to me in that I can’t seem to
> get a crisp image. Does anyone have any experience with reusing dishes?
>
>
>
> Thx!
>
>
>
> Mary

Cha, Byeong

Re: Mattek dishes

That sounds cool. Can you send me your photo of the rings?
Thanks,

Byeong

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Vladimir Gukassyan
Sent: Thursday, November 05, 2009 3:33 PM
To: [hidden email]
Subject: Re: Mattek dishes

Instead of Mattek dishes we're just using a system of metallic rings
and 24mm round coverslips. This is much cheaper solution. Let me know
if you're interested, I'll send you a photo of the rings we have.

With regards,
Vladimir

-----------------------------------------
Vladimir Ghukasyan, Ph.D.
Institute of Biophotonics
National Yang-Ming University
155, Li-Nong St., Sec. 2
Taipei 112, Taiwan
Tel.: (886 2) 28267000 ext. 5709

On Fri, Nov 6, 2009 at 1:46 AM, Jennes, Mary E <[hidden email]> wrote:

> Has anyone ever reused Mattek dishes? I have a user who is trying to save
> some money and is cleaning their dishes with a detergent, rinsing and
> exposing to UV light. The cells look "funny' to me in that I can't seem to
> get a crisp image. Does anyone have any experience with reusing dishes?
>
>
>
> Thx!
>
>
>
> Mary

Mike Tighe

Re: Mattek dishes

In reply to this post by Vladimir Ghukasyan-2

I would also be interested in seeing your set-up!

>>> Vladimir Gukassyan <[hidden email]> 11/05/09 3:32 PM >>>
Instead of Mattek dishes we're just using a system of metallic rings
and 24mm round coverslips. This is much cheaper solution. Let me know
if you're interested, I'll send you a photo of the rings we have.

With regards,
Vladimir

-----------------------------------------
Vladimir Ghukasyan, Ph.D.
Institute of Biophotonics
National Yang- Ming University
155, Li- Nong St., Sec. 2
Taipei 112, Taiwan
Tel.: (886 2) 28267000 ext. 5709

On Fri, Nov 6, 2009 at 1:46 AM, Jennes, Mary E <[hidden email]>
wrote:
> Has anyone ever reused Mattek dishes? I have a user who is trying to
save
> some money and is cleaning their dishes with a detergent, rinsing
and
> exposing to UV light. The cells look “funny’ to me in that I
can’t seem to
> get a crisp image. Does anyone have any experience with reusing
dishes?
>
>
>
> Thx!
>
>
>
> Mary

Craig Brideau

Re: Mattek dishes

I would also like to see the ring thing. @:-)

Craig Brideau
Hotchkiss Brain Institute
University of Calgary

On Thu, Nov 5, 2009 at 1:59 PM, Mike Tighe <[hidden email]> wrote:

> I would also be interested in seeing your set-up!
>
>>>> Vladimir Gukassyan <[hidden email]> 11/05/09 3:32 PM >>>
> Instead of Mattek dishes we're just using a system of metallic rings
> and 24mm round coverslips. This is much cheaper solution. Let me know
> if you're interested, I'll send you a photo of the rings we have.
>
> With regards,
> Vladimir
>
> -----------------------------------------
> Vladimir Ghukasyan, Ph.D.
> Institute of Biophotonics
> National Yang- Ming University
> 155, Li- Nong St., Sec. 2
> Taipei 112, Taiwan
> Tel.: (886 2) 28267000 ext. 5709
>
>
> On Fri, Nov 6, 2009 at 1:46 AM, Jennes, Mary E <[hidden email]>
> wrote:
>> Has anyone ever reused Mattek dishes? I have a user who is trying to
> save
>> some money and is cleaning their dishes with a detergent, rinsing
> and
>> exposing to UV light. The cells look “funny’ to me in that I
> can’t seem to
>> get a crisp image. Does anyone have any experience with reusing
> dishes?
>>
>>
>>
>> Thx!
>>
>>
>>
>> Mary
>

B. Prabhakar Pandian

Re: Mattek dishes

Me Too!

Thanks,

-Prabhakar

Craig Brideau wrote:

> I would also like to see the ring thing. @:-)
>
> Craig Brideau
> Hotchkiss Brain Institute
> University of Calgary
>
>
> On Thu, Nov 5, 2009 at 1:59 PM, Mike Tighe <[hidden email]> wrote:
>
>> I would also be interested in seeing your set-up!
>>
>>
>>>>> Vladimir Gukassyan <[hidden email]> 11/05/09 3:32 PM >>>
>>>>>
>> Instead of Mattek dishes we're just using a system of metallic rings
>> and 24mm round coverslips. This is much cheaper solution. Let me know
>> if you're interested, I'll send you a photo of the rings we have.
>>
>> With regards,
>> Vladimir
>>
>> -----------------------------------------
>> Vladimir Ghukasyan, Ph.D.
>> Institute of Biophotonics
>> National Yang- Ming University
>> 155, Li- Nong St., Sec. 2
>> Taipei 112, Taiwan
>> Tel.: (886 2) 28267000 ext. 5709
>>
>>
>> On Fri, Nov 6, 2009 at 1:46 AM, Jennes, Mary E <[hidden email]>
>> wrote:
>>
>>> Has anyone ever reused Mattek dishes? I have a user who is trying to
>>>
>> save
>>
>>> some money and is cleaning their dishes with a detergent, rinsing
>>>
>> and
>>
>>> exposing to UV light. The cells look “funny’ to me in that I
>>>
>> can’t seem to
>>
>>> get a crisp image. Does anyone have any experience with reusing
>>>
>> dishes?
>>
>>>
>>> Thx!
>>>
>>>
>>>
>>> Mary
>>>
>
>
>

Peter Carroll

Re: Mattek dishes

In reply to this post by Craig Brideau

> I would also like to see the ring thing

id also like to know what you use to cover these, and how good they are
for growing cells...

Craig Brideau wrote:

John Oreopoulos

Re: Mattek dishes

In reply to this post by Craig Brideau

This sounds very similar to the Molecular Probes Attofluor chamber
(no commercial interest):

http://probes.invitrogen.com/media/pis/mp07816.pdf

John Oreopoulos

On 5-Nov-09, at 4:07 PM, Craig Brideau wrote:

> I would also like to see the ring thing. @:-)
>
> Craig Brideau
> Hotchkiss Brain Institute
> University of Calgary
>
>
> On Thu, Nov 5, 2009 at 1:59 PM, Mike Tighe
> <[hidden email]> wrote:
>> I would also be interested in seeing your set-up!
>>
>>>>> Vladimir Gukassyan <[hidden email]> 11/05/09 3:32 PM >>>
>> Instead of Mattek dishes we're just using a system of metallic rings
>> and 24mm round coverslips. This is much cheaper solution. Let me know
>> if you're interested, I'll send you a photo of the rings we have.
>>
>> With regards,
>> Vladimir
>>
>> -----------------------------------------
>> Vladimir Ghukasyan, Ph.D.
>> Institute of Biophotonics
>> National Yang- Ming University
>> 155, Li- Nong St., Sec. 2
>> Taipei 112, Taiwan
>> Tel.: (886 2) 28267000 ext. 5709
>>
>>
>> On Fri, Nov 6, 2009 at 1:46 AM, Jennes, Mary E
>> <[hidden email]>
>> wrote:
>>> Has anyone ever reused Mattek dishes? I have a user who is trying to
>> save
>>> some money and is cleaning their dishes with a detergent, rinsing
>> and
>>> exposing to UV light. The cells look “funny’ to me in that I
>> can’t seem to
>>> get a crisp image. Does anyone have any experience with reusing
>> dishes?
>>>
>>>
>>>
>>> Thx!
>>>
>>>
>>>
>>> Mary
>>

Kristi DeCourcy

Re: Mattek dishes

In reply to this post by Jennes, Mary E

Re: Mattek dishes

Actually, if the user is that...frugal, we used to make our own dishes. All it takes is petris, a small drill press, glass coverslips, and glue. The only thing to be careful about it that you smooth down the drilled edges of the petri, so that the coverslip is flat against the dish bottom when you glue it.

And I still remember my joyous reaction to the discovery that I could buy the same thing from Mattek!

Kristi

Has anyone ever reused Mattek dishes? I have a user who is trying to save some money and is cleaning their dishes with a detergent, rinsing and exposing to UV light. The cells look "funny' to me in that I can't seem to get a crisp image. Does anyone have any experience with reusing dishes?

Thx!

Mary

--

************************************

Kristi R. DeCourcy, Ph.D.
Research Associate/Laboratory Manager
Fralin Life Science Institute
Virginia Tech
West Campus Drive
Blacksburg, VA 24061
Phone: (540) 231-7959
Cell (540) 392-9814
Fax: (540) 231-7126
http://www.biotech.vt.edu

Jennes, Mary E

Re: Mattek dishes

I’ve heard of labs making there own, using the coverslips with the metal rings, but I’ve never know anyone to reuse the dishes. I just need something more concrete to tell the PI other then ‘your cells look funny to me” : (

Thx for all of the input.

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Kristi DeCourcy
Sent: Thursday, November 05, 2009 4:26 PM
To: [hidden email]
Subject: Re: Mattek dishes

And I still remember my joyous reaction to the discovery that I could buy the same thing from Mattek!

Kristi

Has anyone ever reused Mattek dishes? I have a user who is trying to save some money and is cleaning their dishes with a detergent, rinsing and exposing to UV light. The cells look "funny' to me in that I can't seem to get a crisp image. Does anyone have any experience with reusing dishes?

Thx!

Mary

--

Masur, Sandra

Re: Mattek dishes

In reply to this post by Vladimir Ghukasyan-2

I would greatly appreciate the information too.

Best regards,

Sandy,

Sandra K. Masur, PhD
Professor, Ophthalmology
Mount Sinai School of Medicine
Box 1183
1 Gustave Levy Place
New York NY 10029-6574

telephone: 212-241-0089
fax: 212-289-5945

email: [hidden email]

On Nov 5, 2009, at 3:32 PM, Vladimir Gukassyan wrote:

> Instead of Mattek dishes we're just using a system of metallic rings
> and 24mm round coverslips. This is much cheaper solution. Let me know
> if you're interested, I'll send you a photo of the rings we have.
>
> With regards,
> Vladimir
>
> -----------------------------------------
> Vladimir Ghukasyan, Ph.D.
> Institute of Biophotonics
> National Yang-Ming University
> 155, Li-Nong St., Sec. 2
> Taipei 112, Taiwan
> Tel.: (886 2) 28267000 ext. 5709
>
>
> On Fri, Nov 6, 2009 at 1:46 AM, Jennes, Mary E
> <[hidden email]> wrote:
>> Has anyone ever reused Mattek dishes? I have a user who is trying
>> to save
>> some money and is cleaning their dishes with a detergent, rinsing and
>> exposing to UV light. The cells look “funny’ to me in that I can’t
>> seem to
>> get a crisp image. Does anyone have any experience with reusing
>> dishes?
>>
>>
>>
>> Thx!
>>
>>
>>
>> Mary

Craig Brideau

Re: Mattek dishes

In reply to this post by Jennes, Mary E

I agree. Trying to make these things is time consuming.

Craig

On Thu, Nov 5, 2009 at 2:26 PM, Kristi DeCourcy <[hidden email]> wrote:

> Actually, if the user is that...frugal, we used to make our own dishes. All
> it takes is petris, a small drill press, glass coverslips, and glue. The
> only thing to be careful about it that you smooth down the drilled edges of
> the petri, so that the coverslip is flat against the dish bottom when you
> glue it.
> And I still remember my joyous reaction to the discovery that I could buy
> the same thing from Mattek!
> Kristi
>
> Has anyone ever reused Mattek dishes? I have a user who is trying to save
> some money and is cleaning their dishes with a detergent, rinsing and
> exposing to UV light. The cells look "funny' to me in that I can't seem to
> get a crisp image. Does anyone have any experience with reusing dishes?
>
>
>
> Thx!
>
>
>
> Mary
>
> --
>
> ************************************
>
> Kristi R. DeCourcy, Ph.D.
> Research Associate/Laboratory Manager
> Fralin Life Science Institute
> Virginia Tech
> West Campus Drive
> Blacksburg, VA 24061
> Phone: (540) 231-7959
> Cell (540) 392-9814
> Fax: (540) 231-7126
> http://www.biotech.vt.edu

Neeraj Gohad-2

Re: Mattek dishes

In reply to this post by Craig Brideau

I would like to see the rings too!,

Neeraj.

Neeraj V. Gohad, Ph.D.
Postdoctoral Fellow
Okeanos Research Group
Department of Biological Sciences
132 Long Hall
Clemson University
Clemson,SC-29634
Phone: 864-656-3597
Fax: 864-656-0435

Please note my new email address: [hidden email]

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Craig Brideau
Sent: Thursday, November 05, 2009 4:07 PM
To: [hidden email]
Subject: Re: Mattek dishes

I would also like to see the ring thing. @:-)

Craig Brideau
Hotchkiss Brain Institute
University of Calgary

On Thu, Nov 5, 2009 at 1:59 PM, Mike Tighe <[hidden email]> wrote:

> I would also be interested in seeing your set-up!
>
>>>> Vladimir Gukassyan <[hidden email]> 11/05/09 3:32 PM >>>
> Instead of Mattek dishes we're just using a system of metallic rings
> and 24mm round coverslips. This is much cheaper solution. Let me know
> if you're interested, I'll send you a photo of the rings we have.
>
> With regards,
> Vladimir
>
> -----------------------------------------
> Vladimir Ghukasyan, Ph.D.
> Institute of Biophotonics
> National Yang- Ming University
> 155, Li- Nong St., Sec. 2
> Taipei 112, Taiwan
> Tel.: (886 2) 28267000 ext. 5709
>
>
> On Fri, Nov 6, 2009 at 1:46 AM, Jennes, Mary E <[hidden email]>
> wrote:
>> Has anyone ever reused Mattek dishes? I have a user who is trying to
> save
>> some money and is cleaning their dishes with a detergent, rinsing
> and
>> exposing to UV light. The cells look "funny' to me in that I
> can't seem to
>> get a crisp image. Does anyone have any experience with reusing
> dishes?
>>
>>
>>
>> Thx!
>>
>>
>>
>> Mary
>