Micromanager compatibility with Metamorph?

[Holly L. AARON]

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Dear All -

We have been struggling (for some time) with a Nikon/Andor spinning disk
system running Metamorph and I am now wondering about trying MicroManager
on this system. It is in regular use, so we cannot afford to disrupt the
users who are not having issues. One major problem right now is with the
PFS and a difficult sample with a matrix gel and multiple well-plates.

Anyway, my question to the experts out there is whether or not we can run
uManager simultaneously on a system running Metamorph or if they will
interfere with each other and we should not bother?

If they won't play well together, I would be interested to know if there is
a Metamorph User Community out there? Please point me the way! We are not
able to get any support from any of our vendors on this software and feel a
bit stuck with it now.

Thanks!
-Holly
__________________________________________________________________
Holly L. Aaron
Molecular Imaging Center, Cancer Research Laboratory
University of California, Berkeley
251 Life Sciences Addition, MC #2751
Berkeley, CA  94720-2751
510.642.2901 office      510.426.5005 GoogleVoice
[hidden email]      http://imaging.berkeley.edu
Also:
444-B Li Ka Shing Center for Biomedical and Health Sciences

[Kurt Thorn]

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Hi Holly -

There should be no problem with running Metamorph and Micromanager
simultaneously. With very rare exceptions, Micro-manager will run with
the standard drivers provided with the manufacturer. Even if it does
require a special driver, this would have to be installed separately,
and so installing Micro-manager won't touch any of the drivers you are
currently using.

We have a Nikon spinning disk system running both NIS-Elements and
Micro-manager, and we've run several Andor systems under Micro-manager
as well, so I don't think this should be a problem.

Feel free to contact me directly if you want more advice or help with
getting this set up.

Best,
Kurt

On 9/30/2015 10:35 AM, Holly L. AARON wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All -
>
> We have been struggling (for some time) with a Nikon/Andor spinning disk
> system running Metamorph and I am now wondering about trying MicroManager
> on this system. It is in regular use, so we cannot afford to disrupt the
> users who are not having issues. One major problem right now is with the
> PFS and a difficult sample with a matrix gel and multiple well-plates.
>
> Anyway, my question to the experts out there is whether or not we can run
> uManager simultaneously on a system running Metamorph or if they will
> interfere with each other and we should not bother?
>
> If they won't play well together, I would be interested to know if there is
> a Metamorph User Community out there? Please point me the way! We are not
> able to get any support from any of our vendors on this software and feel a
> bit stuck with it now.
>
> Thanks!
> -Holly
> __________________________________________________________________
> Holly L. Aaron
> Molecular Imaging Center, Cancer Research Laboratory
> University of California, Berkeley
> 251 Life Sciences Addition, MC #2751
> Berkeley, CA  94720-2751
> 510.642.2901 office      510.426.5005 GoogleVoice
> [hidden email]      http://imaging.berkeley.edu
> Also:
> 444-B Li Ka Shing Center for Biomedical and Health Sciences
>

--
Kurt Thorn
Associate Professor
Director, Nikon Imaging Center
http://thornlab.ucsf.edu/
http://nic.ucsf.edu/blog/

[Csúcs Gábor-3]

Dear Holly,

Though we didn't do this with a spinning disk system (just a fully motorized wide-field system) we didn't observe any issues having Metamorph and MicoManager on the same machine. Of course you may want to make sure that no one tries to run them at the same time as this may cause trouble. Alternatively, you could set up the system as a dual-boot one and in this case you should be able to avoid any interference.

Greetings   Gabor







-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Holly L. AARON
Sent: Wednesday, September 30, 2015 7:36 PM
To: [hidden email]
Subject: Micromanager compatibility with Metamorph?

*****
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*****

Dear All -

We have been struggling (for some time) with a Nikon/Andor spinning disk system running Metamorph and I am now wondering about trying MicroManager on this system. It is in regular use, so we cannot afford to disrupt the users who are not having issues. One major problem right now is with the PFS and a difficult sample with a matrix gel and multiple well-plates.

Anyway, my question to the experts out there is whether or not we can run uManager simultaneously on a system running Metamorph or if they will interfere with each other and we should not bother?

If they won't play well together, I would be interested to know if there is a Metamorph User Community out there? Please point me the way! We are not able to get any support from any of our vendors on this software and feel a bit stuck with it now.

Thanks!
-Holly
__________________________________________________________________
Holly L. Aaron
Molecular Imaging Center, Cancer Research Laboratory University of California, Berkeley
251 Life Sciences Addition, MC #2751
Berkeley, CA  94720-2751
510.642.2901 office      510.426.5005 GoogleVoice
[hidden email]      http://imaging.berkeley.edu
Also:
444-B Li Ka Shing Center for Biomedical and Health Sciences

[Nico Stuurman-3]

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Hi Holly,

Gabor is absolutely correct.  There is no harm in installing
Micro-Manager side by side with Metamorph.  The only problem I can
imagine is that one of the two would want a different driver version for
a certain piece of hardware .  The easiest way forward is to install
Micro-Manager (which will not install drivers by itself) and see if it
works with your hardware,.

Nevertheless, it is possible that the issues your users with the Perfect
Focus are related to the hardware/sample combination rather than the
software.

Best,

Nico


On 9/30/15 11:44 AM, Csúcs Gábor wrote:

> Dear Holly,
>
> Though we didn't do this with a spinning disk system (just a fully motorized wide-field system) we didn't observe any issues having Metamorph and MicoManager on the same machine. Of course you may want to make sure that no one tries to run them at the same time as this may cause trouble. Alternatively, you could set up the system as a dual-boot one and in this case you should be able to avoid any interference.
>
> Greetings   Gabor
>
>
>
>
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Holly L. AARON
> Sent: Wednesday, September 30, 2015 7:36 PM
> To: [hidden email]
> Subject: Micromanager compatibility with Metamorph?
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All -
>
> We have been struggling (for some time) with a Nikon/Andor spinning disk system running Metamorph and I am now wondering about trying MicroManager on this system. It is in regular use, so we cannot afford to disrupt the users who are not having issues. One major problem right now is with the PFS and a difficult sample with a matrix gel and multiple well-plates.
>
> Anyway, my question to the experts out there is whether or not we can run uManager simultaneously on a system running Metamorph or if they will interfere with each other and we should not bother?
>
> If they won't play well together, I would be interested to know if there is a Metamorph User Community out there? Please point me the way! We are not able to get any support from any of our vendors on this software and feel a bit stuck with it now.
>
> Thanks!
> -Holly
> __________________________________________________________________
> Holly L. Aaron
> Molecular Imaging Center, Cancer Research Laboratory University of California, Berkeley
> 251 Life Sciences Addition, MC #2751
> Berkeley, CA  94720-2751
> 510.642.2901 office      510.426.5005 GoogleVoice
> [hidden email]      http://imaging.berkeley.edu
> Also:
> 444-B Li Ka Shing Center for Biomedical and Health Sciences

--
Nico Stuurman
Vale lab, UCSF/HHMI
Genentech Hall, N316, MC2200
600 - 16th Street
San Francisco, CA 94158-2517
415 514 3927
[hidden email]

[G. Esteban Fernandez]

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For what it's worth, I have a Leica widefield and a Zeiss with 3rd party
spinning disk that I run with Micro-Manager or manufacturer software
installed on the same system. I have never experienced interference unless
I tried to run both programs simultaneously.

-Esteban
On Sep 30, 2015 11:56 AM, "Kurt Thorn" <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Holly -
>
> There should be no problem with running Metamorph and Micromanager
> simultaneously. With very rare exceptions, Micro-manager will run with the
> standard drivers provided with the manufacturer. Even if it does require a
> special driver, this would have to be installed separately, and so
> installing Micro-manager won't touch any of the drivers you are currently
> using.
>
> We have a Nikon spinning disk system running both NIS-Elements and
> Micro-manager, and we've run several Andor systems under Micro-manager as
> well, so I don't think this should be a problem.
>
> Feel free to contact me directly if you want more advice or help with
> getting this set up.
>
> Best,
> Kurt
>
> On 9/30/2015 10:35 AM, Holly L. AARON wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Dear All -
>>
>> We have been struggling (for some time) with a Nikon/Andor spinning disk
>> system running Metamorph and I am now wondering about trying MicroManager
>> on this system. It is in regular use, so we cannot afford to disrupt the
>> users who are not having issues. One major problem right now is with the
>> PFS and a difficult sample with a matrix gel and multiple well-plates.
>>
>> Anyway, my question to the experts out there is whether or not we can run
>> uManager simultaneously on a system running Metamorph or if they will
>> interfere with each other and we should not bother?
>>
>> If they won't play well together, I would be interested to know if there
>> is
>> a Metamorph User Community out there? Please point me the way! We are not
>> able to get any support from any of our vendors on this software and feel
>> a
>> bit stuck with it now.
>>
>> Thanks!
>> -Holly
>> __________________________________________________________________
>> Holly L. Aaron
>> Molecular Imaging Center, Cancer Research Laboratory
>> University of California, Berkeley
>> 251 Life Sciences Addition, MC #2751
>> Berkeley, CA  94720-2751
>> 510.642.2901 office      510.426.5005 GoogleVoice
>> [hidden email]      http://imaging.berkeley.edu
>> Also:
>> 444-B Li Ka Shing Center for Biomedical and Health Sciences
>>
>>
>
> --
> Kurt Thorn
> Associate Professor
> Director, Nikon Imaging Center
> http://thornlab.ucsf.edu/
> http://nic.ucsf.edu/blog/
>

[Pariksheet Nanda]

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To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Holly,

On Wed, Sep 30, 2015 at 5:35 PM, Holly L. AARON <[hidden email]> wrote:
> I would be interested to know if there is
> a Metamorph User Community out there? Please point me the way! We are not
> able to get any support from any of our vendors on this software and feel a
> bit stuck with it now.

Since no one has mentioned it yet, I just thought I'd point out that,
starting today, commercial support for Micro-Manager is available
directly from the developers through Open-Imaging
(https://open-imaging.com/).  Additionally, Micro-Manager has an
excellent public community through the mailing list
(https://micro-manager.org/wiki/Micro-Manager%20Community).


> -Holly

Pariksheet

[WAINWRIGHT James]

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*****

*** COMMERCIAL RESPONSE ***

Hi Holly,

I'm a microscopy support engineer for Andor in the UK and have some (limited) experience running our microscopy systems under uManager.

Please feel free to contact me off-list if you need any technical information on setting up any of your hardware supplied from Andor.

Best wishes!

James


James Wainwright
Product Support Engineer (Microscopy Systems)
Europe, Middle East, Africa

Tel: [phone]
Mob: [phone]
Fax: [phone]
Web:  andor.com/support



________________________________________
From: Confocal Microscopy List [[hidden email]] on behalf of Pariksheet Nanda [[hidden email]]
Sent: 02 October 2015 03:42
To: [hidden email]
Subject: Re: Micromanager compatibility with Metamorph?

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Holly,

On Wed, Sep 30, 2015 at 5:35 PM, Holly L. AARON <[hidden email]> wrote:
> I would be interested to know if there is
> a Metamorph User Community out there? Please point me the way! We are not
> able to get any support from any of our vendors on this software and feel a
> bit stuck with it now.

Since no one has mentioned it yet, I just thought I'd point out that,
starting today, commercial support for Micro-Manager is available
directly from the developers through Open-Imaging
(https://open-imaging.com/).  Additionally, Micro-Manager has an
excellent public community through the mailing list
(https://micro-manager.org/wiki/Micro-Manager%20Community).


> -Holly

Pariksheet


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