Multi-user Imaging room plans?
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milton charlton |
Multi-user Imaging room plans?
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We are contemplating
building a multi-user imaging room containing 3 confocals, 2
2-photon confocals, 1-2 widefield scopes. We would isolate parts of
the room with curtains.
Has anyone
constructed such a room and can send plans and/or recommendations about
construction, utilities, etc? Photos might help too.
Thanks for your
help.
Milton
Charlton
Milton P. Charlton, Professor |
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Paul Herzmark |
Fwd: Multi-user Imaging room plans?
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Milton, Here is a bunch of ideas I used when I planned a new imaging facility. Paul Herzmark Specialist [hidden email] Department of Molecular and Cell Biology 479 Life Science Addition University of California, Berkeley Berkeley, CA 94720-3200 (510) 643-9603 (510) 643-9500 fax Here are are some things to keep in mind based on my experience setting up microscopy facilities. The manufactures should give you information for the specific instruments (especially the footprint and electrical requirements). Electricity: Minimum of eight duplex outlets at each microscope with separate circuits for arc lamps and lasers from electronics. Most of the outlets should be above the desk level for easy access. Lighting: Dark areas around each microscope. Black curtains work fine. Lighting that can be dimmed. Controls near the microscope or additional plug-in lamps. Networking: Two internet connections for each microscope; one for the computer, one for a personal laptop. Maybe wireless? Telephone: Access at each microscope for discussion with service people. A wireless portable phone that can reach each microscope is sufficient. General utilities: Drop electricity, gas, vacuum etc from the ceiling onto the table so you can walk completely around without jumping over wires,etc. Space: Enough space for the microscope and peripherals. Space for several people around the microscope in order to use it as a teaching lab. Space next to each microscope for a work table and desk top for the computer. At least 18 inches clearance behind the computer/microscope tables for access to cables. Wall space for white boards, posters. You may want to locate the lasers in a separate room for noise, ventilation, vibration and cooling purposes. Temperature control/ventilation: Sufficient cooling for lamps, lasers, electronics, multiple people. (chilled water for cooling lasers?) There is probably no need to have the space under negative or positive air pressure. Plumbing: House air, house vacuum at each scope for air tables, aspirating samples. I don't think you need gas. Sample preparation: Space, electricity, plumbing for tissue culture hood, CO2 incubator, (incubators for bacteria, yeast?), table top centrifuge, sink, work space and some storage space. The hood will need a vacuum line. I don't think you need gas. The incubator will need CO2. You might need an emergency eyewash and shower here. Storage: Some shelves near each microscope for equipment manuals, etc. Some users may have accessories that they can not easily transport each time they come. You may consider providing some drawer space for users. Space for tools. Security: card key access to the center, but probably not necessary for access to individual microscopes. Miscellaneous: Work space for equipment repair. Office space. Get it all approved by the Department of Environmental Health and Safety. On Tue, Feb 3, 2009 at 9:46 AM, Milton Charlton <[hidden email]> wrote:
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Tim O'Brien Sr. |
Re: Multi-user Imaging room plans?
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In reply to this post by milton charlton
We did a bit of that when we moved to our new lab, with one large room
subdivided with curtains, and one with actual walls and doors to make
minilabs within. Our experience seems to be that the walled rooms,
although more expensive, are routinely used for independent
experiments, while the curtained room is less easily used for
independent experiments. One issue is separate lights for each area.
Depending on how much input you have into construction, if you should
aim to have separate and independent control of the lighting for each
subdivided area, you might be able to make it practical for separate
users. We also often wish we had separate light switches in each area
as well as by the main door. It is also harder to separate one's self
from conversation and so on within a curtained area. Minimize traffic
if possible. You also need to have a path to walk or move things
between areas without disturbing the others. Some microscopes need
access to the rear and not just the front (for lasers and so on), and
that needs to be thought though. Plus curtains are not as noise-damping
as walls. Nor as light excluding.
So, I would recommend really thinking through the daily use of a subdivided area, and, if possible, to actually subdivide with walls and (large) doors, with independent light and power and air supply for each mini-room. And you do not want the air supply vents to be sending air at the microscope, but have a way to direct it away. Or at least independently lit curtained areas. With that many nice microscopes, you will get a lot more out of them if they are separated by real walls. A nice problem to have! Tim O'Brien CISMM, UNC Chapel Hill Milton Charlton wrote:
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Marc Thibault |
Re: Multi-user Imaging room plans?
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In reply to this post by milton charlton
HI, We found Microsoft Visio to be very useful to have scalable
plans of the room to optimise layout of the equipments. M ------------------------------------------------------------------------ Marc Thibault, Ph.D. NSERC/Biosynthech Chair in Hybrid Biomaterials for Innovative Regenerative Technologies Department of Chemical Engineering Ecole Polytechnique, Montreal Tel: 514 340 4711 (3968) ------------------------------------------------------------------------ From: Milton Charlton
[mailto:[hidden email]] We
are contemplating building a multi-user imaging room containing 3
confocals, 2 2-photon confocals, 1-2 widefield scopes. We
would isolate parts of the room with curtains. Has
anyone constructed such a room and can send plans and/or recommendations about
construction, utilities, etc? Photos might help too. Thanks
for your help. Milton
Charlton Milton P.
Charlton, Professor |
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Boswell, Carl A - (cboswell) |
Re: Multi-user Imaging room plans?
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In reply to this post by milton charlton
Aside from stray light issues, do your homework
regarding the electrical needs for each instrument (including outlet/plug
compatibility) and the heat output, usually identified as total
watts. It will be imperitive that your room has the air handling capacity
to move the heat generated by all those instruments out of the room. High
ceilings help.
Large capacity uninterruptable power supplies (UPS)
would be crucial also. I've had good luck with Powerware hardware, and
Power Pros www.powerproinc.com, have
been very helpful with custom setups. Unless you have GaAs PMT's, there is
no need to paint the room black.
Carl
Carl A. Boswell, Ph.D.
Molecular and Cellular Biology University of Arizona 520-954-7053 FAX 520-621-3709
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Craig Brideau |
Re: Multi-user Imaging room plans?
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We have a fairly large multi-user room that is subdivided into four compartments with curtains. I second what everyone else has said an make the following recommendations:
Paint the walls matte black. We found this made a huge difference in containing stray light leakage around the curtains, especially light from computer monitors. For the lighting, have dimmable pot lights for each curtained zone with sliders in the zone to dim the lights. Just on/off control is not enough; often you want slight lighting and that's where the dimmers come in handy. Combining this with on/off fluorescent lighting is also handy; use the bright fluorescent lights when doing setup and the like, then switch to the pot lights for experiments. For the curtains; triple layer -two heavy black walls with a heavy duty fire-retardant core, especially if you are using ultrafast lasers for two photon or similar. A chilled water supply may be useful for removing excess heat from specific equipment. If you put a copper tube serpentine by the exhaust vents of a particularly hot piece of equipment and circulate chilled water you can keep control of point heat sources. Otherwise just make sure you have ample air conditioning. Remember that people are significant sources of heat in addition to equipment! Make sure air intakes and outputs are well diffused; stray air currents from the ventilation can cause problems with vibration. You need excellent temperature and humidity control. Humidity should be maintained from 20-40% and temperature should be stable to two degrees or better. Have a ready supply of small cheap flashlights handy! Put a small table/cabinet outside the room with common supplies so people can get organized before entering the room. Keep a supply of lens tissue and cleaner by each microscope. We bought dim phosphorescent sticky paper; I cut strips off of them and stuck them to the corners of all the tables and by the exits in the rooms. When the lights first go out they glow dimly enough to show you where the edges are so you don't run into things, but not so brightly that it interferes with your experiments. By the time they have run out of glow (last about an hour after exposure to fluorescent lighting for a few hours) the users eyes have dark adjusted anyway. By the best passive damped anti-vibration tables you can afford. This is especially critical if the lab is not in a basement, or if there are sources of noise nearby. The active systems are useful in extreme cases but usually just thick tables (18") and gas shocks will handle most issues. Shipping and receiving access! Do you have access to cargo elevators and sufficiently wide doors to easily get equipment and tables in and out of the rooms? Our lab section didn't have a proper cargo elevator so our anti-vibration tables had to be swung in by crane. Not cheap! Hope this helps! Craig On Tue, Feb 3, 2009 at 12:00 PM, Carl Boswell <[hidden email]> wrote:
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Martin Wessendorf-2 |
Re: Multi-user Imaging room plans?
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In reply to this post by milton charlton
Dear Dr. Charlton--
Milton Charlton wrote: > We are contemplating building a multi-user imaging room containing 3 > confocals, 2 2-photon confocals, 1-2 widefield scopes. We would > isolate parts of the room with curtains. > Has anyone constructed such a room and can send plans and/or > recommendations about construction, utilities, etc? Photos might help too. > Thanks for your help. One suggestion: be very careful about how the air conditioning is set up. Depending upon (1) the temperature differential between ambient air and the air conditioning outflow, and (2) the placement of the air conditioning duct relative to your microscope, you can produce VERY noticeable (--like 3 um) shifts in focus/stage position. The resulting 3D reconstructions look pretty odd. Martin -- Martin Wessendorf, Ph.D. office: (612) 626-0145 Assoc Prof, Dept Neuroscience lab: (612) 624-2991 University of Minnesota Preferred FAX: (612) 624-8118 6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009 Minneapolis, MN 55455 **MY E-MAIL ADDRESS HAS CHANGED. PLEASE USE [hidden email] ** |
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Rosemary.White |
Re: Multi-user Imaging room plans?
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In reply to this post by Boswell, Carl A - (cboswell)
speaking from depressing experience, Rosemary Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia ph 61 2 6246 5475 fx 61 2 6246 5334 On 4/02/09 6:00 AM, "Carl Boswell" <cboswell@...> wrote: Aside from stray light issues, do your homework regarding the electrical needs for each instrument (including outlet/plug compatibility) and the heat output, usually identified as total watts. It will be imperitive that your room has the air handling capacity to move the heat generated by all those instruments out of the room. High ceilings help. |
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Craig Brideau |
Re: Multi-user Imaging room plans?
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As an added bonus, UPS systems also filter your power which can reduce electronic noise...
Craig On Tue, Feb 3, 2009 at 2:42 PM, Rosemary White <[hidden email]> wrote:
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Donnelly, Tom |
Re: Multi-user Imaging room plans?
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In reply to this post by Martin Wessendorf-2
Amen to that!!
I have seen huge drift XY and Z with poor temperature control. An AC or Heat vent blowing on the system can create havoc on imaging and deconvolution. We had a case that we named "dot boogie" where a bead would "boogie" out of the field of view (60x) and boogie back in depending if the AC was blowing or not. Z was also "interesting". Cheers, Tom -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Martin Wessendorf Sent: Tuesday, February 03, 2009 12:39 PM To: [hidden email] Subject: Re: Multi-user Imaging room plans? Dear Dr. Charlton-- Milton Charlton wrote: > We are contemplating building a multi-user imaging room containing 3 > confocals, 2 2-photon confocals, 1-2 widefield scopes. We would > isolate parts of the room with curtains. > Has anyone constructed such a room and can send plans and/or > recommendations about construction, utilities, etc? Photos might help too. > Thanks for your help. One suggestion: be very careful about how the air conditioning is set up. Depending upon (1) the temperature differential between ambient air and the air conditioning outflow, and (2) the placement of the air conditioning duct relative to your microscope, you can produce VERY noticeable (--like 3 um) shifts in focus/stage position. The resulting 3D reconstructions look pretty odd. Martin -- Martin Wessendorf, Ph.D. office: (612) 626-0145 Assoc Prof, Dept Neuroscience lab: (612) 624-2991 University of Minnesota Preferred FAX: (612) 624-8118 6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009 Minneapolis, MN 55455 **MY E-MAIL ADDRESS HAS CHANGED. PLEASE USE [hidden email] ** |
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Gabriel Lapointe |
Re: Multi-user Imaging room plans?
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In reply to this post by Martin Wessendorf-2
Be also very careful where you install air conditioning vents AND
thermostat. Our confocal (with lasers) is with on one side of the curtain and the cool air on the other side. Results in a very warm working station and a freezing hallway. My advice on this is to consider each curtained area as a separate room with closed doors. Gabriel Lapointe Martin Wessendorf wrote: > Dear Dr. Charlton-- > > Milton Charlton wrote: >> We are contemplating building a multi-user imaging room containing 3 >> confocals, 2 2-photon confocals, 1-2 widefield scopes. We would >> isolate parts of the room with curtains. >> Has anyone constructed such a room and can send plans and/or >> recommendations about construction, utilities, etc? Photos might >> help too. >> Thanks for your help. > > One suggestion: be very careful about how the air conditioning is set > up. Depending upon (1) the temperature differential between ambient > air and the air conditioning outflow, and (2) the placement of the air > conditioning duct relative to your microscope, you can produce VERY > noticeable (--like 3 um) shifts in focus/stage position. The > resulting 3D reconstructions look pretty odd. > > Martin |
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Craig Brideau |
Re: Multi-user Imaging room plans?
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We actually have a temperature and humidity sensor for each curtained zone on a long cable rather than a fixed thermostat. We can move the sensor around each curtained area to get an even result for each zone.
Craig On Tue, Feb 3, 2009 at 3:42 PM, Gabriel Lapointe <[hidden email]> wrote: Be also very careful where you install air conditioning vents AND |
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Zucker.Robert |
Re: Fwd: Multi-user Imaging room plans?
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In reply to this post by Paul Herzmark
A number of factors can cause instability in a confocal microscope.
Many of these variables can be addressed and confocal instability eliminated with proper room design. It is unbelievable but true that many confocal microscopes normally have 10-20% Laser power fluctuations. If the room temperature is not stable, an instability in the AOTF and laser power can occur . It is critical to keep the temperature in the room constant so the components of the microscope and lasers will not drift.. Two recent Cytometry reprints that describe confocal performance and instability are the following and maybe helpful information on this subject. 1. Zucker RM Confocal Slide Based System :Performance. Cytometry 2006 69 A 659-676.. 2. Zucker RM Confocal Microscopy Slide Based Systems: Instability. Cytometry 2006 69A 677-690. Reprints are available on request. bob Robert M. Zucker, PhD U.S. Environmental Protection Agency Office of Research and Development National Health and Environmental Effects Research Laboratory. Reproductive Toxicology Division Telephone: 919-541-1585 Fax: 919-541-4017 e-mail: [hidden email] Mail address: USEPA,ORD,NHEERL,RTD Developmental Biology Branch ( MD 67) Research Triangle Park, North Carolina, 27711 Shipping address: 2525 E.NC Highway 54 Durham, NC, 27713 |------------> | From: | |------------> >-------------------------------------------------------------------------------------------------------------------------------------| |Paul Herzmark <[hidden email]> | >-------------------------------------------------------------------------------------------------------------------------------------| |------------> | To: | |------------> >-------------------------------------------------------------------------------------------------------------------------------------| |[hidden email] | >-------------------------------------------------------------------------------------------------------------------------------------| |------------> | Date: | |------------> >-------------------------------------------------------------------------------------------------------------------------------------| |02/03/2009 01:10 PM | >-------------------------------------------------------------------------------------------------------------------------------------| |------------> | Subject: | |------------> >-------------------------------------------------------------------------------------------------------------------------------------| |Fwd: Multi-user Imaging room plans? | >-------------------------------------------------------------------------------------------------------------------------------------| Milton, Here is a bunch of ideas I used when I planned a new imaging facility. Paul Herzmark Specialist [hidden email] Department of Molecular and Cell Biology 479 Life Science Addition University of California, Berkeley Berkeley, CA 94720-3200 (510) 643-9603 (510) 643-9500 fax Here are are some things to keep in mind based on my experience setting up microscopy facilities. The manufactures should give you information for the specific instruments (especially the footprint and electrical requirements). Electricity: Minimum of eight duplex outlets at each microscope with separate circuits for arc lamps and lasers from electronics. Most of the outlets should be above the desk level for easy access. Lighting: Dark areas around each microscope. Black curtains work fine. Lighting that can be dimmed. Controls near the microscope or additional plug-in lamps. Networking: Two internet connections for each microscope; one for the computer, one for a personal laptop. Maybe wireless? Telephone: Access at each microscope for discussion with service people. A wireless portable phone that can reach each microscope is sufficient. General utilities: Drop electricity, gas, vacuum etc from the ceiling onto the table so you can walk completely around without jumping over wires,etc. Space: Enough space for the microscope and peripherals. Space for several people around the microscope in order to use it as a teaching lab. Space next to each microscope for a work table and desk top for the computer. At least 18 inches clearance behind the computer/microscope tables for access to cables. Wall space for white boards, posters. You may want to locate the lasers in a separate room for noise, ventilation, vibration and cooling purposes. Temperature control/ventilation: Sufficient cooling for lamps, lasers, electronics, multiple people. (chilled water for cooling lasers?) There is probably no need to have the space under negative or positive air pressure. Plumbing: House air, house vacuum at each scope for air tables, aspirating samples. I don't think you need gas. Sample preparation: Space, electricity, plumbing for tissue culture hood, CO2 incubator, (incubators for bacteria, yeast?), table top centrifuge, sink, work space and some storage space. The hood will need a vacuum line. I don't think you need gas. The incubator will need CO2. You might need an emergency eyewash and shower here. Storage: Some shelves near each microscope for equipment manuals, etc. Some users may have accessories that they can not easily transport each time they come. You may consider providing some drawer space for users. Space for tools. Security: card key access to the center, but probably not necessary for access to individual microscopes. Miscellaneous: Work space for equipment repair. Office space. Get it all approved by the Department of Environmental Health and Safety. On Tue, Feb 3, 2009 at 9:46 AM, Milton Charlton < [hidden email]> wrote: We are contemplating building a multi-user imaging room containing 3 confocals, 2 2-photon confocals, 1-2 widefield scopes. We would isolate parts of the room with curtains. Has anyone constructed such a room and can send plans and/or recommendations about construction, utilities, etc? Photos might help too. Thanks for your help. Milton Charlton Milton P. Charlton, Professor Physiology Department Medical Science Building #3308 University of Toronto 1 King's College Circle Toronto, ON, M5S1A8 Canada tel: 416-978-6355 fax: 416-978-4940 [hidden email] |
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In reply to this post by Tim O'Brien Sr.
I have to agree with that. We've had multiple
confocals and widefield systems
in one room without too much trouble (independent lighting
controls of course)
but multiphoton scopes really need darkroom
conditions. You're going to
have real trouble with just curtains when one user is
trying to take images
and another is turning lights on to adjust their live-cell
incubation equipment.
Guy
Optical Imaging Techniques in Cell Biology From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of E. Tim O'Brien Sent: Wednesday, 4 February 2009 5:33 AM To: [hidden email] Subject: Re: Multi-user Imaging room plans? So, I would recommend really thinking through the daily use of a subdivided area, and, if possible, to actually subdivide with walls and (large) doors, with independent light and power and air supply for each mini-room. And you do not want the air supply vents to be sending air at the microscope, but have a way to direct it away. Or at least independently lit curtained areas. With that many nice microscopes, you will get a lot more out of them if they are separated by real walls. A nice problem to have! Tim O'Brien CISMM, UNC Chapel Hill Milton Charlton wrote:
No virus found in this incoming message. No virus found in this outgoing message. |
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Sylvie Le Guyader-2 |
Re: Multi-user Imaging room plans?
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In reply to this post by milton charlton
Hi Milton I agree with all that was said. I’d
add that we designed our room so that all sides of all microscopes are accessible.
We fiddle behind the equipment many times per day. We built very sturdy self
standing shelves above each microscope table for the heat generating equipment
to lie onto. Above each shelf is a hood that gently channels the heat away from
the room. Our room has 5 curtained areas, each with
one hood, one main lamp and one small lamp. The 5 main lamps can be turned
on/off near the main door but they can be also disabled centrally with a key so
that no one turns the main on accidentally during someone else’s
acquisition. One more thing you need to consider: if
you are planning on using your two photon scopes for whole animals, you might
be required to have a double door, negative pressure, some working space… Med vänlig hälsning / Best
regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader Dept of Biosciences and Nutrition Karolinska Institutet Novum 14157 +46 (0)8 608 9240 From:
Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Milton Charlton We are contemplating building a multi-user imaging room
containing 3 confocals, 2 2-photon confocals, 1-2 widefield
scopes. We would isolate parts of the room with curtains. Has anyone constructed such a room and can send plans and/or
recommendations about construction, utilities, etc? Photos might help
too. Thanks for your help. Milton Charlton Milton P.
Charlton, Professor |
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In reply to this post by milton charlton
Hello:
If you want to minimize temperature fluctuations and are using an air-cooled argon ion laser, vent it elsewhere! I wish I would have thought of that. Someone else mentioned it on the list a while back. Beware of safety requirements for the Class 4 lasers. Laser curtains could be required. Good luck! Sophie ____________________________________________________ Sophie M. K. Brunet, Ph. D. Research Officer Optical Spectroscopy, Laser Systems and Applications [hidden email] 306-966-1719 (office) 306-966-1702 (fax) ____________________________________________________ Saskatchewan Structural Sciences Centre University of Saskatchewan Thorvaldson Bldg. 110 Science Place Saskatoon, Sk S7N 5C9 ____________________________________________________ Quoting Milton Charlton <[hidden email]>: > We are contemplating building a multi-user imaging room containing 3 > confocals, 2 2-photon confocals, 1-2 widefield scopes. We would isolate > parts of the room with curtains. > Has anyone constructed such a room and can send plans and/or recommendations > about construction, utilities, etc? Photos might help too. > Thanks for your help. > > Milton Charlton > > > Milton P. Charlton, Professor > Physiology Department > Medical Science Building #3308 > University of Toronto > 1 King's College Circle > Toronto, ON, M5S1A8 > Canada > tel: 416-978-6355 > fax: 416-978-4940 > [hidden email] > > > > > |
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Erik Manders-2 |
Re: Multi-user Imaging room plans?
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In reply to this post by milton charlton
Just some tips:
On each microscope we have a Oko-lab incubator with black panels (this Italian company calls the "obscuring panels"; these incubators are very stable and dedicated for the research environment). So, no curtains in our microscopy rooms (3 mics per room). And we can work with the light on!! We will move to a new building this year. Between two microscope rooms we will have a small extra room where we will put all the equipment that produces heat and/or noise. Long fibers between the microscopes and laser room. This laser room will be temperature controled (some extra cooling units). So, no warm, noisy, dark room with dusty curtains! Let the sunshine in! Good luck, Erik Manders |
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Erik,
You cannot in any circumstances work with multi-photon scopes with the light on. Sure, 3 confocal or widefield scopes in one room can work with no curtains (actually we have 5) with local control of lights, but NOT multiphoton scopes. Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope Unit, Madsen Building F09, University of Sydney, NSW 2006 ______________________________________________ Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Erik Manders Sent: Saturday, 7 February 2009 10:14 PM To: [hidden email] Subject: Re: Multi-user Imaging room plans? Just some tips: On each microscope we have a Oko-lab incubator with black panels (this Italian company calls the "obscuring panels"; these incubators are very stable and dedicated for the research environment). So, no curtains in our microscopy rooms (3 mics per room). And we can work with the light on!! We will move to a new building this year. Between two microscope rooms we will have a small extra room where we will put all the equipment that produces heat and/or noise. Long fibers between the microscopes and laser room. This laser room will be temperature controled (some extra cooling units). So, no warm, noisy, dark room with dusty curtains! Let the sunshine in! Good luck, Erik Manders No virus found in this incoming message. Checked by AVG. Version: 7.5.552 / Virus Database: 270.10.19/1939 - Release Date: 7/02/2009 1:39 PM No virus found in this outgoing message. Checked by AVG. Version: 7.5.552 / Virus Database: 270.10.19/1939 - Release Date: 7/02/2009 1:39 PM |
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Patrick Van Oostveldt |
Re: Multi-user Imaging room plans?
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Dear,
I would advice fluorescence microscopy users to work in a dark room not to protect their samples but to be adapted to low light levels if you like to see fluorescent cells at low light levels through the binocular. An other option is the use of specific red light as is used in photographic dark rooms. However if all is covered and you only have a digital image transmitted over the internet, a dark room is not necessary, you than can even use the microscope of your neighborgh Bye Patrick Van Oostveldt Quoting Guy Cox <[hidden email]>: > Erik, > > You cannot in any circumstances work with > multi-photon scopes with the light on. Sure, 3 confocal > or widefield scopes in one room can work with no curtains > (actually we have 5) with local control of lights, but > NOT multiphoton scopes. > > Guy > > > > Optical Imaging Techniques in Cell Biology > by Guy Cox CRC Press / Taylor & Francis > http://www.guycox.com/optical.htm > ______________________________________________ > Associate Professor Guy Cox, MA, DPhil(Oxon) > Electron Microscope Unit, Madsen Building F09, > University of Sydney, NSW 2006 > ______________________________________________ > Phone +61 2 9351 3176 Fax +61 2 9351 7682 > Mobile 0413 281 861 > ______________________________________________ > http://www.guycox.net > -----Original Message----- > From: Confocal Microscopy List > [mailto:[hidden email]] On Behalf Of Erik Manders > Sent: Saturday, 7 February 2009 10:14 PM > To: [hidden email] > Subject: Re: Multi-user Imaging room plans? > > Just some tips: > On each microscope we have a Oko-lab incubator with black panels > (this Italian company calls the "obscuring panels"; these incubators > are very stable and dedicated for the research environment). So, no > curtains in our microscopy rooms (3 mics per room). And we can work > with the light on!! > We will move to a new building this year. Between two microscope > rooms we will have a small extra room where we will put all the > equipment that produces heat and/or noise. Long fibers between the > microscopes and laser room. This laser room will be temperature > controled (some extra cooling units). So, no warm, noisy, dark room > with dusty curtains! Let the sunshine in! > Good luck, Erik Manders > > No virus found in this incoming message. > Checked by AVG. > Version: 7.5.552 / Virus Database: 270.10.19/1939 - Release Date: > 7/02/2009 1:39 PM > > > No virus found in this outgoing message. > Checked by AVG. > Version: 7.5.552 / Virus Database: 270.10.19/1939 - Release Date: > 7/02/2009 1:39 PM > > -- Dep. Moleculaire Biotechnologie Coupure links 653 B 9000 GENT tel 09 264 5969 fax 09 264 6219 |
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Adrian Smith-6 |
Re: Multi-user Imaging room plans?
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In reply to this post by Guy Cox
Hi Guy,
You can always put the entire multiphoton scope (minus lasers) in a light proof box... ie functionally placing it inside its own room ;)...but a room you don't have to be sitting in... I think Prairie Technologies has been doing that for a while and LaVision BioTec built such enclosure for our TriMScope and it works very well. Most of the researchers here still like to turn the room light down or off (force of habit plus a "better safe than sorry" attitude) but on the couple of occasions I've done a direct test with room light on/off I've not been able to show any significant difference between the images. At the very least it allows us not to worry about light from the computer screens impacting on the detectors. Regards, Adrian Smith Centenary Institute, Sydney, Australia On 08/02/2009, at 6:32 PM, Guy Cox wrote: > Erik, > > You cannot in any circumstances work with > multi-photon scopes with the light on. Sure, 3 confocal > or widefield scopes in one room can work with no curtains > (actually we have 5) with local control of lights, but > NOT multiphoton scopes. > > Guy > > > > Optical Imaging Techniques in Cell Biology > by Guy Cox CRC Press / Taylor & Francis > http://www.guycox.com/optical.htm > ______________________________________________ > Associate Professor Guy Cox, MA, DPhil(Oxon) > Electron Microscope Unit, Madsen Building F09, > University of Sydney, NSW 2006 > ______________________________________________ > Phone +61 2 9351 3176 Fax +61 2 9351 7682 > Mobile 0413 281 861 > ______________________________________________ > http://www.guycox.net > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email] > ] On Behalf Of Erik Manders > Sent: Saturday, 7 February 2009 10:14 PM > To: [hidden email] > Subject: Re: Multi-user Imaging room plans? > > Just some tips: > On each microscope we have a Oko-lab incubator with black panels > (this Italian company calls the "obscuring panels"; these incubators > are very stable and dedicated for the research environment). So, no > curtains in our microscopy rooms (3 mics per room). And we can work > with the light on!! > We will move to a new building this year. Between two microscope > rooms we will have a small extra room where we will put all the > equipment that produces heat and/or noise. Long fibers between the > microscopes and laser room. This laser room will be temperature > controled (some extra cooling units). So, no warm, noisy, dark room > with dusty curtains! Let the sunshine in! > Good luck, Erik Manders > > No virus found in this incoming message. > Checked by AVG. > Version: 7.5.552 / Virus Database: 270.10.19/1939 - Release Date: > 7/02/2009 1:39 PM > > > No virus found in this outgoing message. > Checked by AVG. > Version: 7.5.552 / Virus Database: 270.10.19/1939 - Release Date: > 7/02/2009 1:39 PM > |
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