MultiOmyx technology?

[Gary Laevsky]

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Hi All,

Does anyone have any experience with this system?  Preliminary interest
here but would like some real world feedback.  Thanks as always.

--
Best,

Gary Laevsky, Ph.D.
Confocal Imaging Facility Manager
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310

[DamirSudar]

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Hi Gary,

I am very interested to hear what you find out. We had some early
discussions with them but were shocked by the cost: they indicated pricing
along the lines of $100K-150K for 10 markers on 10-20 slides. Too expensive
by far so we didn't proceed.

Thanks,
- Damir

On Wed, Jul 8, 2015 at 2:49 AM, Gary Laevsky <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi All,
>
> Does anyone have any experience with this system?  Preliminary interest
> here but would like some real world feedback.  Thanks as always.
>
> --
> Best,
>
> Gary Laevsky, Ph.D.
> Confocal Imaging Facility Manager
> Dept. of Molecular Biology
> Washington Rd.
> Princeton University
> Princeton, New Jersey, 08544-1014
> (O) 609 258 5432
> (C) 508 507 1310
>

--
Damir Sudar - Staff Scientist
Lawrence Berkeley Laboratory / Life Sciences Division
One Cyclotron Road, MS 977, Berkeley, CA 94720, USA
T: 510/486-5346 - F: 510/486-5586 - E: [hidden email]

Visiting Scientist, Oregon Health and Science University

[Christian-103]

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List,
Our 8k+ hour Argon laser gave up last week and we need to replace it with a diode unit and need pointers.  We're not going to miss the extra laser lines, but need the 488nm.  Furthermore, what suggestions are there for the old laser head?  We really do not need it cluttering up the lab.  Simplistic I know, but we need to keep that FluoView 500 ticking along and this is beyond me.
Thanks.
Christian

[Craig Brideau]

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I've worn out a couple argon lasers and considered diode lasers as
replacements in both cases. We actually make use of the 514 and 457 lines
fairly regularly. Replacing the argon with three diode lasers was pretty
cost prohibitive, and it turns out that having our old argons refurbished
only cost around $5k. I went with these guys:
http://www.modu-laser.com/
They have refurbished two argons for me and I've been very pleased with
their work in both cases.

Craig

On Wed, Jul 8, 2015 at 10:40 AM, Christian <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> List,
> Our 8k+ hour Argon laser gave up last week and we need to replace it with
> a diode unit and need pointers.  We're not going to miss the extra laser
> lines, but need the 488nm.  Furthermore, what suggestions are there for the
> old laser head?  We really do not need it cluttering up the lab.
> Simplistic I know, but we need to keep that FluoView 500 ticking along and
> this is beyond me.
> Thanks.
> Christian
>
>

[Jens]

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Hi Christian,

You might not go this way but building some simple diode lasers is actually quite easy. Companies like Thorlabs sell the diodes as well as mounts and driving electronics.
Osram for example produces pretty cheap 450 and 520 nm diodes with powers of 50 mW upwards. The 488nm diode range is still expensive so I went with a 470 nm DPSS laser instead which works fine for GFP.
I found that a spatial filter based on a Keplerian beam expander with a Pinhole in the middle cleans up the beam profile a lot.
Fiber-coupling might be a little more complicated.

Best,

Jens

> On Jul 8, 2015, at 1:41 PM, Craig Brideau <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> I've worn out a couple argon lasers and considered diode lasers as
> replacements in both cases. We actually make use of the 514 and 457 lines
> fairly regularly. Replacing the argon with three diode lasers was pretty
> cost prohibitive, and it turns out that having our old argons refurbished
> only cost around $5k. I went with these guys:
> http://www.modu-laser.com/
> They have refurbished two argons for me and I've been very pleased with
> their work in both cases.
>
> Craig
>
> On Wed, Jul 8, 2015 at 10:40 AM, Christian <[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> List,
>> Our 8k+ hour Argon laser gave up last week and we need to replace it with
>> a diode unit and need pointers.  We're not going to miss the extra laser
>> lines, but need the 488nm.  Furthermore, what suggestions are there for the
>> old laser head?  We really do not need it cluttering up the lab.
>> Simplistic I know, but we need to keep that FluoView 500 ticking along and
>> this is beyond me.
>> Thanks.
>> Christian
>>
>>