Multiline HeNe
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hello, I am looking for a supplier of multiwavelength HeNe (543, 594, 633nm simultaneous). I contacted a few, but was not successful. Does anyone know (or even the old part number for it). Thank you, Sophie _________________________________________________ Sophie M.K. Brunet, Ph.D. University of Saskatchewan, Canada |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Apparently REO still makes them: http://www.reoinc.com/products/hene_lasers/Multi-line/ It's not clear if this is a true simultaneous multiline, or if it's a tuneable model so you'll want to confirm with the company. I take it you need very narrow line width and/or very specific wavelengths which is why you are not considering diodes or solid state lasers? (nice to see another Canuck on the forum by the way!) Craig On Wed, Nov 5, 2014 at 6:29 PM, Brunet, Sophie <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hello, > I am looking for a supplier of multiwavelength HeNe (543, 594, 633nm > simultaneous). > I contacted a few, but was not successful. > Does anyone know (or even the old part number for it). > Thank you, > Sophie > _________________________________________________ > Sophie M.K. Brunet, Ph.D. > University of Saskatchewan, Canada > |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear all, I am trying to monitor ROS production by different treatments on different cell lines using DCFH-DA (dichlorofluorescein diacetate), the method is quite well established and is commonly used with plate readers and FACS (http://www.ncbi.nlm.nih.gov/pubmed/10490282) but… looking at the loaded cells in wide field fluorescence I start with very dim fluorescence levels that increase, over time, by more than 10 times; in 15-30 seconds the cells reach a sort of plateau and then photobleaching takes over and the fluorescence returns slowly to low levels. Different cell lines behave slightly differently in the timing of the process. I was thinking about a sort of photo activation process but literature does not help too much. Of course in these conditions is impossible to establish a correlation between ROS production and fluorescence levels. Anybody experienced this phenomenon? Any suggestion? Thanks, best regards Stefano Stefano Giovannardi PhD Cell Physiology Lab Dipartimento di Scienze Teoriche e Applicate Universita' dell'Insubria via A. da Giussano, 12 21052 Busto Arsizio (VA), Italy [hidden email] www.thepsychophonicnurse.it |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear Stefano, You are probably using much too much excitation light and you need to observe with a low-light camera, not the naked eye. Consider using chloromethyl-DCF, which forms covalent bonds with cell proteins and is better retained after cells are injured. John --- John J. Lemasters, MD, PhD Professor and GlaxoSmithKline Distinguished Endowed Chair Director, Center for Cell Death, Injury & Regeneration Departments of Drug Discovery & Biomedical Sciences and Biochemistry & Molecular Biology Medical University of South Carolina DD504 Drug Discovery Building 70 President Street, MSC 140 Charleston, SC 29425 Email: [hidden email] -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Stefano Giovannardi Sent: Thursday, November 6, 2014 5:32 AM To: [hidden email] Subject: ROS detection ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear all, I am trying to monitor ROS production by different treatments on different cell lines using DCFH-DA (dichlorofluorescein diacetate), the method is quite well established and is commonly used with plate readers and FACS (http://www.ncbi.nlm.nih.gov/pubmed/10490282) but... looking at the loaded cells in wide field fluorescence I start with very dim fluorescence levels that increase, over time, by more than 10 times; in 15-30 seconds the cells reach a sort of plateau and then photobleaching takes over and the fluorescence returns slowly to low levels. Different cell lines behave slightly differently in the timing of the process. I was thinking about a sort of photo activation process but literature does not help too much. Of course in these conditions is impossible to establish a correlation between ROS production and fluorescence levels. Anybody experienced this phenomenon? Any suggestion? Thanks, best regards Stefano Stefano Giovannardi PhD Cell Physiology Lab Dipartimento di Scienze Teoriche e Applicate Universita' dell'Insubria via A. da Giussano, 12 21052 Busto Arsizio (VA), Italy [hidden email] www.thepsychophonicnurse.it |
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In reply to this post by Brunet, Sophie
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi, The REO is a tunable laser. I really want this as an educational tool (low power for low safety + low budget). I want to show what a grating does. A single wavelength does not do a good job and a Ar+ ($, power/safety, usually 20amperes). I am waiting to hear if I am able to buy second-hand. I know that the HeNe was mentioned in this list before. Thank you, Sophie _________________________________________________ Sophie M.K. Brunet, Ph.D. University of Saskatchewan, Canada |
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