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Multiphoton spectra of fluorescent dyes

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Gary G. Li-2

Multiphoton spectra of fluorescent dyes

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Greetings,

For one photon excitation, the excitation and emission spectra of commonly
used fluorescent dyes can be viewed by using the following tools:

* Invitrogen Fluorescence SpectraViewer, www.invitrogen.com

* BD Fluorescence Spectrum Viewer, www.bdbiosciences.com

I am wondering if we have similar tools for fluorophores with multiphoton
excitation.

Many thanks

Gary G. Li, Ph.D.

Microscopy Core Manager

NIH/NIDA

333 Cassell Drive, Suite 2100

Baltimore, MD 21224

[hidden email]

[hidden email] <[hidden email]>

<[hidden email]>

Farid Jalali

Re: Multiphoton spectra of fluorescent dyes

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Hello Gary,

I have found the DRBIO website (Developmental Resource for Biophysical
Imaging Opto-Electronics at Cornell- Warren Zipfel, Watt Webb and crew
) at http://www.drbio.cornell.edu/cross_sections.html to be an
excellent resource for at least the 2P excitation cross sections. The
following paper is good commonly used reagents. I have yet to find
anything equivalent to the resources you mention however.

Two-photon fluorescence absorption and emission spectra of dyes
relevant for cell imaging.
J Microsc. 2002 Nov;208(Pt 2):108-15
Bestvater F, Spiess E, Stobrawa G, Hacker M, Feurer T, Porwol T,
Berchner-Pfannschmidt U, Wotzlaw C, Acker H.

Cheers
Farid

On Tue, Apr 5, 2011 at 8:03 AM, Gary G. Li <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Greetings,
>
> For one photon excitation, the excitation and emission spectra of commonly
> used fluorescent dyes can be viewed by using the following tools:
>
> * Invitrogen Fluorescence SpectraViewer, www.invitrogen.com
>
> * BD Fluorescence Spectrum Viewer, www.bdbiosciences.com
>
> I am wondering if we have similar tools for fluorophores with multiphoton
> excitation.
>
> Many thanks
>
> Gary G. Li, Ph.D.
>
> Microscopy Core Manager
>
> NIH/NIDA
>
> 333 Cassell Drive, Suite 2100
>
> Baltimore, MD 21224
>
> [hidden email]
>
> [hidden email] <[hidden email]>
>
>
> <[hidden email]>
>

Johannes Helm

Re: Multiphoton spectra of fluorescent dyes

In reply to this post by Gary G. Li-2

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Good afternoon,

while I am not aware of a similar on line tool for multi photon
excitations, there are publications, which list "MP" excitation spectra of
a number of dyes, e.g. by Bestvater et al.

Ref.

Bestvater, F. et al.
Two-photon fluorescence absorption and emission spectra of
dyes relevant for cell imaging,
Journal of Microscopy, Vol. 208, Pt 2 November 2002, pp. 108115

Best wishes,

Johannes

--
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Seniorengineer
CMBN
University of Oslo
Institute of Basic Medical Science
Department of Anatomy
Postboks 1105 - Blindern
NO-0317 Oslo

Voice: +47 228 51159
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WWW: folk.uio.no/jhelm

James Fitzpatrick

Re: Multiphoton spectra of fluorescent dyes

In reply to this post by Gary G. Li-2

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Gary,

Have you tried the DRBIO resource at Cornell that is run by Warren Zipfel now? They have a list of two-photon absorption cross-sections there that I find helpful. Its not exhaustive, but they have a number of the fluorescent proteins and alexa dyes listed there for example, and you can download the ascii data as well.

http://www.drbio.cornell.edu/cross_sections.html

Best wishes,
James.

On Apr 5, 2011, at 8:03 AM, Gary G. Li wrote:

-----
James A.J. Fitzpatrick, Ph.D.
Core Director
Waitt Advanced Biophotonics Center
The Salk Institute for Biological Studies
10010 North Torrey Pines Road
La Jolla, CA 92037

(t): +1 858-453-4100 x2119
(e): [hidden email]
(w): http:/www.salk.edu/biophotonics_core

Boswell, Carl A - (cboswell)

Re: Multiphoton spectra of fluorescent dyes

In reply to this post by Farid Jalali

Probably the most comprehensive site would be at: http://www.spectra.arizona.edu/ . We've compiled all the 2P data we can find, including the DRBIO data. Simply select "2-photon cross-sections" under Dye category. We hope to incorporate the ability to download any data the user chooses, soon. This site has been assembled by Dr. Urs Utzinger at the U of A Biomedical Engineering Dept. and myself, with the code-writing help of Gary Carstensen in our Instruction/Access office, and includes much of the 1P data collected by Dr. George McNamara, U. of Miami, and myself.

Additional spectral data from optics, dyes, filters and light sources always welcome.

Cheers,
C

Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
Univ. of Arizona
520-954-7053
FAX 520-621-3709

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Farid Jalali
Sent: Tuesday, April 05, 2011 8:30 AM
To: [hidden email]
Subject: Re: Multiphoton spectra of fluorescent dyes

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hello Gary,

I have found the DRBIO website (Developmental Resource for Biophysical Imaging Opto-Electronics at Cornell- Warren Zipfel, Watt Webb and crew
) at http://www.drbio.cornell.edu/cross_sections.html to be an excellent resource for at least the 2P excitation cross sections. The following paper is good commonly used reagents. I have yet to find anything equivalent to the resources you mention however.

Two-photon fluorescence absorption and emission spectra of dyes relevant for cell imaging.
J Microsc. 2002 Nov;208(Pt 2):108-15
Bestvater F, Spiess E, Stobrawa G, Hacker M, Feurer T, Porwol T, Berchner-Pfannschmidt U, Wotzlaw C, Acker H.

Cheers
Farid

On Tue, Apr 5, 2011 at 8:03 AM, Gary G. Li <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Greetings,
>
> For one photon excitation, the excitation and emission spectra of
> commonly used fluorescent dyes can be viewed by using the following tools:
>
> * Invitrogen Fluorescence SpectraViewer, www.invitrogen.com
>
> * BD Fluorescence Spectrum Viewer, www.bdbiosciences.com
>
> I am wondering if we have similar tools for fluorophores with
> multiphoton excitation.
>
> Many thanks
>
> Gary G. Li, Ph.D.
>
> Microscopy Core Manager
>
> NIH/NIDA
>
> 333 Cassell Drive, Suite 2100
>
> Baltimore, MD 21224
>
> [hidden email]
>
> [hidden email] <[hidden email]>
>
>
> <[hidden email]>
>

John Oreopoulos

Re: Multiphoton spectra of fluorescent dyes

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Two other publications of interest for you:

Dickinson, M.E., et al., Multiphoton excitation spectra in biological samples. Journal of Biomedical Optics, 2003. 8(3): p. 329-338.
Spiess, E., et al., Two-photon excitation and emission spectra of the green fluorescent protein variants ecfp, egfp and eyfp. Journal of Microscopy-Oxford, 2005. 217: p. 200-204.

John Oreopoulos
Research Assistant
Spectral Applied Research
Richmond Hill, Ontario
Canada
www.spectral.ca

On 2011-04-05, at 7:46 PM, Boswell, Carl A - (cboswell) wrote:

> Probably the most comprehensive site would be at: http://www.spectra.arizona.edu/ . We've compiled all the 2P data we can find, including the DRBIO data. Simply select "2-photon cross-sections" under Dye category. We hope to incorporate the ability to download any data the user chooses, soon. This site has been assembled by Dr. Urs Utzinger at the U of A Biomedical Engineering Dept. and myself, with the code-writing help of Gary Carstensen in our Instruction/Access office, and includes much of the 1P data collected by Dr. George McNamara, U. of Miami, and myself.
>
> Additional spectral data from optics, dyes, filters and light sources always welcome.
>
> Cheers,
> C
>
>
>
> Carl A. Boswell, Ph.D.
> Molecular and Cellular Biology
> Univ. of Arizona
> 520-954-7053
> FAX 520-621-3709
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Farid Jalali
> Sent: Tuesday, April 05, 2011 8:30 AM
> To: [hidden email]
> Subject: Re: Multiphoton spectra of fluorescent dyes
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello Gary,
>
> I have found the DRBIO website (Developmental Resource for Biophysical Imaging Opto-Electronics at Cornell- Warren Zipfel, Watt Webb and crew
> ) at http://www.drbio.cornell.edu/cross_sections.html to be an excellent resource for at least the 2P excitation cross sections. The following paper is good commonly used reagents. I have yet to find anything equivalent to the resources you mention however.
>
> Two-photon fluorescence absorption and emission spectra of dyes relevant for cell imaging.
> J Microsc. 2002 Nov;208(Pt 2):108-15
> Bestvater F, Spiess E, Stobrawa G, Hacker M, Feurer T, Porwol T, Berchner-Pfannschmidt U, Wotzlaw C, Acker H.
>
>
> Cheers
> Farid
>
> On Tue, Apr 5, 2011 at 8:03 AM, Gary G. Li <[hidden email]> wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Greetings,
>>
>> For one photon excitation, the excitation and emission spectra of
>> commonly used fluorescent dyes can be viewed by using the following tools:
>>
>> * Invitrogen Fluorescence SpectraViewer, www.invitrogen.com
>>
>> * BD Fluorescence Spectrum Viewer, www.bdbiosciences.com
>>
>> I am wondering if we have similar tools for fluorophores with
>> multiphoton excitation.
>>
>> Many thanks
>>
>> Gary G. Li, Ph.D.
>>
>> Microscopy Core Manager
>>
>> NIH/NIDA
>>
>> 333 Cassell Drive, Suite 2100
>>
>> Baltimore, MD 21224
>>
>> [hidden email]
>>
>> [hidden email] <[hidden email]>
>>
>>
>> <[hidden email]>
>>
>

Marco Dal Maschio

Re: Multiphoton spectra of fluorescent dyes

In reply to this post by Gary G. Li-2

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

let me suggest for reddish emitting molecules
the following paper where are resumed 9 crosssections
including tdtomato, mcherry, mRFP and dsred2.

Absolute two-photon absorption spectra and two-photon brightness of orange
and red fluorescent proteins. <http://www.ncbi.nlm.nih.gov/pubmed/19127988>

Drobizhev M, Tillo S, Makarov NS, Hughes TE, Rebane A.

J Phys Chem B. 2009 Jan 29;113(4):855-9.

My best
Marco

--
Marco Dal Maschio, PhD
PostDoc fellow
Neuroscience and Brain Technologies Dept
Italian Institute of Technology
via Morego 30, Genova, Italy

Boswell, Carl A - (cboswell)

Re: Multiphoton spectra of fluorescent dyes

These have been incorporated into the database.
C

Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
Univ. of Arizona
520-954-7053
FAX 520-621-3709

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Marco Dal Maschio
Sent: Wednesday, April 06, 2011 12:50 AM
To: [hidden email]
Subject: Re: Multiphoton spectra of fluorescent dyes

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

let me suggest for reddish emitting molecules the following paper where are resumed 9 crosssections including tdtomato, mcherry, mRFP and dsred2.

Absolute two-photon absorption spectra and two-photon brightness of orange and red fluorescent proteins. <http://www.ncbi.nlm.nih.gov/pubmed/19127988>

Drobizhev M, Tillo S, Makarov NS, Hughes TE, Rebane A.

J Phys Chem B. 2009 Jan 29;113(4):855-9.

My best
Marco

--
Marco Dal Maschio, PhD
PostDoc fellow
Neuroscience and Brain Technologies Dept Italian Institute of Technology via Morego 30, Genova, Italy