Mystery microscope

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mmodel mmodel
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Mystery microscope

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Dear colleagues... (Sorry if you are getting this message second time, I am having some trouble sending to the Confocal List)
 
I was at the Microscience meeting in London in 2008, and in one vendor booth they were telling (not really displaying because they didn't have it with them) about a strange microscope that achieves a very good resolution in transmission, better than the diffraction limit, and a large depth of field. They didn't explain how it works. I think the company was based in Germany, but I don't remember the name.  Does anyone know what it could have been or the company name? I would like to contact them. Thanks!
 
Mike Model
Kent State University
Barbara Foster Barbara Foster
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Re: Mystery microscope

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Hi, Mike

I'm not sure about the one at the Microscience meeting, but there is
an interesting holographic microscope which is just now coming out of
Canada ... and I know that they've already installed one at the U of
Heidleberg in Germany.  It is the DIHM (Digital In-situ Holographic
Microscope).  The company is Resolution Optics
(ResolutionOptics.com).  It not only has a great DOF, it can track
motion in 3D and is available in both a desktop model and a
retrofittable system based on an objective (objective, point source,
software that works with a set of staging and camera options).  They
also have a submersible version that works down to depths of 5 km (3miles!).

I saw them at M&M.  Their CEO Is Stefan Jericho ([hidden email])

Hope this was helpful,
Barbara Foster, President and Sr. Consultant

Microscopy/Microscopy Education
7101 Royal Glen Trail, Suite A
McKinney TX 75070
P: (972)924-5310  Skype: fostermme
W: www.MicroscopyEducation.com

NEWS! Visit the NEW and IMPROVED www.MicroscopyEducation.com! And
don't forget:  MME is now scheduling customized, on-site courses for
the Fall and Winter.  Call me for a free assessment and quote.

At 10:57 AM 10/3/2010, you wrote

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>Dear colleagues... (Sorry if you are getting this message second
>time, I am having some trouble sending to the Confocal List)
>
>I was at the Microscience meeting in London in 2008, and in one
>vendor booth they were telling (not really displaying because they
>didn't have it with them) about a strange microscope that achieves a
>very good resolution in transmission, better than the diffraction
>limit, and a large depth of field. They didn't explain how it works.
>I think the company was based in Germany, but I don't remember the
>name.  Does anyone know what it could have been or the company name?
>I would like to contact them. Thanks!
>
>Mike Model
>Kent State University
Peter Humphreys Peter Humphreys
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Re: Mystery microscope

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Phasefocus maybe ?

Peter Humphreys
Centre for Stem Cell Research
Cambridge


> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear colleagues... (Sorry if you are getting this message second time, I am having some trouble sending to the Confocal List)
>  
> I was at the Microscience meeting in London in 2008, and in one vendor booth they were telling (not really displaying because they didn't have it with them) about a strange microscope that achieves a very good resolution in transmission, better than the diffraction limit, and a large depth of field. They didn't explain how it works. I think the company was based in Germany, but I don't remember the name.  Does anyone know what it could have been or the company name? I would like to contact them. Thanks!
>  
> Mike Model
> Kent State University
O'Toole, P O'Toole, P
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Re: Mystery microscope

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  Was it the Richardson Technologies instrument? This appeared to
resolve unstained mitochondria through the transmitted light path.  Not
sure that it could collect a large depth of field and also thought it
was 2004 or 2006 that they were present.

Phase Focus is a different system again that can 'virtual' focus through
z once a x,y tile has been collected.  This system uses coherent light
and collects the light in the transmission light path.

Best

Pete

On 03/10/2010 17:56, MODEL, MICHAEL wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear colleagues... (Sorry if you are getting this message second time, I am having some trouble sending to the Confocal List)
>
> I was at the Microscience meeting in London in 2008, and in one vendor booth they were telling (not really displaying because they didn't have it with them) about a strange microscope that achieves a very good resolution in transmission, better than the diffraction limit, and a large depth of field. They didn't explain how it works. I think the company was based in Germany, but I don't remember the name.  Does anyone know what it could have been or the company name? I would like to contact them. Thanks!
>
> Mike Model
> Kent State University

--
Dr Peter O'Toole
Head of Imaging and Cytometry
Technology Facility
Department of Biology (Area 15)
University of York
YORK
YO10 5DD

Tel : +44 (0)1904 328722
Fax : +44 (0)1904 328804
email : [hidden email]
www.york.ac.uk/biology/tf

EMAIL DISCLAIMER http://www.york.ac.uk/docs/disclaimer/email.htm
Judy Trogadis-2 Judy Trogadis-2
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Re: Mystery microscope

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Yes, it does sound like the Richardson microscope. It has no oculars. They also used an oil coupled condensor. Dr. Richardson sold the company and the patents to someone else. I can try to find out who is selling them now, I know one of their ex-employees.
Judy


Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 7Queen
30 Bond St.
Toronto, ON M5B 1W8, Canada
ph:  416-864-6060  x6337
pager: 416-685-9219
fax: 416-864-5046
[hidden email]


>>> "O'Toole, P" <[hidden email]> 10/4/2010 4:43 AM >>>
*****
To join, leave or search the confocal microscopy listserv, go to:
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*****

  Was it the Richardson Technologies instrument? This appeared to
resolve unstained mitochondria through the transmitted light path.  Not
sure that it could collect a large depth of field and also thought it
was 2004 or 2006 that they were present.

Phase Focus is a different system again that can 'virtual' focus through
z once a x,y tile has been collected.  This system uses coherent light
and collects the light in the transmission light path.

Best

Pete

On 03/10/2010 17:56, MODEL, MICHAEL wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy 
> *****
>
> Dear colleagues... (Sorry if you are getting this message second time, I am having some trouble sending to the Confocal List)
>
> I was at the Microscience meeting in London in 2008, and in one vendor booth they were telling (not really displaying because they didn't have it with them) about a strange microscope that achieves a very good resolution in transmission, better than the diffraction limit, and a large depth of field. They didn't explain how it works. I think the company was based in Germany, but I don't remember the name.  Does anyone know what it could have been or the company name? I would like to contact them. Thanks!
>
> Mike Model
> Kent State University

--
Dr Peter O'Toole
Head of Imaging and Cytometry
Technology Facility
Department of Biology (Area 15)
University of York
YORK
YO10 5DD

Tel : +44 (0)1904 328722
Fax : +44 (0)1904 328804
email : [hidden email]
www.york.ac.uk/biology/tf

EMAIL DISCLAIMER http://www.york.ac.uk/docs/disclaimer/email.htm
Teng-Leong Chew Teng-Leong Chew
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Re: Mystery microscope

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I think the Richardson scope is a good guess. Dark field, perhaps?

Leong

--
Teng-Leong Chew, Ph.D.
Director, Cell Imaging Facility & Nikon Imaging Center
Director of University Imaging Resources
Feinberg School of Medicine & Office of Research
Northwestern University
303 E. Chicago Avenue
Chicago, IL 60611
(312) 503-2841
(847) 491-7091 (W, F)


On 10/4/10 8:21 AM, "Judy Trogadis" <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Yes, it does sound like the Richardson microscope. It has no oculars. They
> also used an oil coupled condensor. Dr. Richardson sold the company and the
> patents to someone else. I can try to find out who is selling them now, I know
> one of their ex-employees.
> Judy
>
>
> Judy Trogadis
> Bio-Imaging Coordinator
> St. Michael's Hospital, 7Queen
> 30 Bond St.
> Toronto, ON M5B 1W8, Canada
> ph:  416-864-6060  x6337
> pager: 416-685-9219
> fax: 416-864-5046
> [hidden email]
>
>
>>>> "O'Toole, P" <[hidden email]> 10/4/2010 4:43 AM >>>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
>   Was it the Richardson Technologies instrument? This appeared to
> resolve unstained mitochondria through the transmitted light path.  Not
> sure that it could collect a large depth of field and also thought it
> was 2004 or 2006 that they were present.
>
> Phase Focus is a different system again that can 'virtual' focus through
> z once a x,y tile has been collected.  This system uses coherent light
> and collects the light in the transmission light path.
>
> Best
>
> Pete
>
> On 03/10/2010 17:56, MODEL, MICHAEL wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Dear colleagues... (Sorry if you are getting this message second time, I am
>> having some trouble sending to the Confocal List)
>>
>> I was at the Microscience meeting in London in 2008, and in one vendor booth
>> they were telling (not really displaying because they didn't have it with
>> them) about a strange microscope that achieves a very good resolution in
>> transmission, better than the diffraction limit, and a large depth of field.
>> They didn't explain how it works. I think the company was based in Germany,
>> but I don't remember the name.  Does anyone know what it could have been or
>> the company name? I would like to contact them. Thanks!
>>
>> Mike Model
>> Kent State University

--
Teng-Leong Chew, Ph.D.
Director, Cell Imaging Facility & Nikon Imaging Center
Director of University Imaging Resources
Feinberg School of Medicine & Office of Research
Northwestern University
303 E. Chicago Avenue
Chicago, IL 60611
(312) 503-2841
(847) 491-7091 (W, F)
mmodel mmodel
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Re: Mystery microscope

In reply to this post by Judy Trogadis-2
*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Thanks for all the responses from which I have already learned many new things. I remember they were saying that it was based on some principle that had never been published or patented. I also think (though not 100% certain) that it worked in all the common transmission modes. But all they had was a brochure..



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Judy Trogadis
Sent: Monday, October 04, 2010 9:22 AM
To: [hidden email]
Subject: Re: Mystery microscope

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Yes, it does sound like the Richardson microscope. It has no oculars. They also used an oil coupled condensor. Dr. Richardson sold the company and the patents to someone else. I can try to find out who is selling them now, I know one of their ex-employees.
Judy


Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 7Queen
30 Bond St.
Toronto, ON M5B 1W8, Canada
ph:  416-864-6060  x6337
pager: 416-685-9219
fax: 416-864-5046
[hidden email]


>>> "O'Toole, P" <[hidden email]> 10/4/2010 4:43 AM >>>
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy 
*****

  Was it the Richardson Technologies instrument? This appeared to
resolve unstained mitochondria through the transmitted light path.  Not
sure that it could collect a large depth of field and also thought it
was 2004 or 2006 that they were present.

Phase Focus is a different system again that can 'virtual' focus through
z once a x,y tile has been collected.  This system uses coherent light
and collects the light in the transmission light path.

Best

Pete

On 03/10/2010 17:56, MODEL, MICHAEL wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy 
> *****
>
> Dear colleagues... (Sorry if you are getting this message second time, I am having some trouble sending to the Confocal List)
>
> I was at the Microscience meeting in London in 2008, and in one vendor booth they were telling (not really displaying because they didn't have it with them) about a strange microscope that achieves a very good resolution in transmission, better than the diffraction limit, and a large depth of field. They didn't explain how it works. I think the company was based in Germany, but I don't remember the name.  Does anyone know what it could have been or the company name? I would like to contact them. Thanks!
>
> Mike Model
> Kent State University

--
Dr Peter O'Toole
Head of Imaging and Cytometry
Technology Facility
Department of Biology (Area 15)
University of York
YORK
YO10 5DD

Tel : +44 (0)1904 328722
Fax : +44 (0)1904 328804
email : [hidden email]
www.york.ac.uk/biology/tf

EMAIL DISCLAIMER http://www.york.ac.uk/docs/disclaimer/email.htm
Judy Trogadis-2 Judy Trogadis-2
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Re: Mystery microscope

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*****

Dr. Richardson kept everything very secret, wouldn't say how it worked. Perhaps not the best approach when selling to scientists  :-)
Judy


>>> "MODEL, MICHAEL" <[hidden email]> 10/4/2010 10:55 AM >>>
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy 
*****

Thanks for all the responses from which I have already learned many new things. I remember they were saying that it was based on some principle that had never been published or patented. I also think (though not 100% certain) that it worked in all the common transmission modes. But all they had was a brochure..



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Judy Trogadis
Sent: Monday, October 04, 2010 9:22 AM
To: [hidden email]
Subject: Re: Mystery microscope

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy 
*****

Yes, it does sound like the Richardson microscope. It has no oculars. They also used an oil coupled condensor. Dr. Richardson sold the company and the patents to someone else. I can try to find out who is selling them now, I know one of their ex-employees.
Judy


Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 7Queen
30 Bond St.
Toronto, ON M5B 1W8, Canada
ph:  416-864-6060  x6337
pager: 416-685-9219
fax: 416-864-5046
[hidden email]


>>> "O'Toole, P" <[hidden email]> 10/4/2010 4:43 AM >>>
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy 
*****

  Was it the Richardson Technologies instrument? This appeared to
resolve unstained mitochondria through the transmitted light path.  Not
sure that it could collect a large depth of field and also thought it
was 2004 or 2006 that they were present.

Phase Focus is a different system again that can 'virtual' focus through
z once a x,y tile has been collected.  This system uses coherent light
and collects the light in the transmission light path.

Best

Pete

On 03/10/2010 17:56, MODEL, MICHAEL wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy 
> *****
>
> Dear colleagues... (Sorry if you are getting this message second time, I am having some trouble sending to the Confocal List)
>
> I was at the Microscience meeting in London in 2008, and in one vendor booth they were telling (not really displaying because they didn't have it with them) about a strange microscope that achieves a very good resolution in transmission, better than the diffraction limit, and a large depth of field. They didn't explain how it works. I think the company was based in Germany, but I don't remember the name.  Does anyone know what it could have been or the company name? I would like to contact them. Thanks!
>
> Mike Model
> Kent State University

--
Dr Peter O'Toole
Head of Imaging and Cytometry
Technology Facility
Department of Biology (Area 15)
University of York
YORK
YO10 5DD

Tel : +44 (0)1904 328722
Fax : +44 (0)1904 328804
email : [hidden email]
www.york.ac.uk/biology/tf

EMAIL DISCLAIMER http://www.york.ac.uk/docs/disclaimer/email.htm
Barbara Foster Barbara Foster
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Re: Mystery microscope

In reply to this post by O'Toole, P
*****
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Hi, Pete

To the best of my knowledge, Richardson Technologies has been out of
business for a number of years now.

Best regards,
Barbara Foster, President and Sr. Consultant

Microscopy/Microscopy Education
7101 Royal Glen Trail, Suite A
McKinney TX 75070
P: (972)924-5310  Skype: fostermme
W: www.MicroscopyEducation.com

NEWS! Visit the NEW and IMPROVED www.MicroscopyEducation.com! And
don't forget:  MME is now scheduling customized, on-site courses for
the Fall and Winter.  Call me for a free assessment and quote.



At 09:57 PM 10/3/2010, O'Toole, P wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>  Was it the Richardson Technologies instrument? This appeared to
> resolve unstained mitochondria through the transmitted light
> path.  Not sure that it could collect a large depth of field and
> also thought it was 2004 or 2006 that they were present.
>
>Phase Focus is a different system again that can 'virtual' focus
>through z once a x,y tile has been collected.  This system uses
>coherent light and collects the light in the transmission light path.
>
>Best
>
>Pete
>
>On 03/10/2010 17:56, MODEL, MICHAEL wrote:
>>*****
>>To join, leave or search the confocal microscopy listserv, go to:
>>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>*****
>>
>>Dear colleagues... (Sorry if you are getting this message second
>>time, I am having some trouble sending to the Confocal List)
>>
>>I was at the Microscience meeting in London in 2008, and in one
>>vendor booth they were telling (not really displaying because they
>>didn't have it with them) about a strange microscope that achieves
>>a very good resolution in transmission, better than the diffraction
>>limit, and a large depth of field. They didn't explain how it
>>works. I think the company was based in Germany, but I don't
>>remember the name.  Does anyone know what it could have been or the
>>company name? I would like to contact them. Thanks!
>>
>>Mike Model
>>Kent State University
>
>--
>Dr Peter O'Toole
>Head of Imaging and Cytometry
>Technology Facility
>Department of Biology (Area 15)
>University of York
>YORK
>YO10 5DD
>
>Tel : +44 (0)1904 328722
>Fax : +44 (0)1904 328804
>email : [hidden email]
>www.york.ac.uk/biology/tf
>
>EMAIL DISCLAIMER http://www.york.ac.uk/docs/disclaimer/email.htm
Robin Battye Robin Battye
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Re: Mystery microscope

In reply to this post by Judy Trogadis-2
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Quorum technologies in Canada


Cheers Robin

Robin Battye, M.Sc., Ph.D.
Vice President Sales and Development
[hidden email]
www.quorumtechnologies.com
Cell: (647) 285-9998
Office: (519) 824-0854
Fax: (519) 824-5845

On 2010-10-04, at 7:21, Judy Trogadis <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Yes, it does sound like the Richardson microscope. It has no oculars. They also used an oil coupled condensor. Dr. Richardson sold the company and the patents to someone else. I can try to find out who is selling them now, I know one of their ex-employees.
> Judy
>
>
> Judy Trogadis
> Bio-Imaging Coordinator
> St. Michael's Hospital, 7Queen
> 30 Bond St.
> Toronto, ON M5B 1W8, Canada
> ph:  416-864-6060  x6337
> pager: 416-685-9219
> fax: 416-864-5046
> [hidden email]
>
>
>>>> "O'Toole, P" <[hidden email]> 10/4/2010 4:43 AM >>>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy 
> *****
>
>  Was it the Richardson Technologies instrument? This appeared to
> resolve unstained mitochondria through the transmitted light path.  Not
> sure that it could collect a large depth of field and also thought it
> was 2004 or 2006 that they were present.
>
> Phase Focus is a different system again that can 'virtual' focus through
> z once a x,y tile has been collected.  This system uses coherent light
> and collects the light in the transmission light path.
>
> Best
>
> Pete
>
> On 03/10/2010 17:56, MODEL, MICHAEL wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy 
>> *****
>>
>> Dear colleagues... (Sorry if you are getting this message second time, I am having some trouble sending to the Confocal List)
>>
>> I was at the Microscience meeting in London in 2008, and in one vendor booth they were telling (not really displaying because they didn't have it with them) about a strange microscope that achieves a very good resolution in transmission, better than the diffraction limit, and a large depth of field. They didn't explain how it works. I think the company was based in Germany, but I don't remember the name.  Does anyone know what it could have been or the company name? I would like to contact them. Thanks!
>>
>> Mike Model
>> Kent State University
>
> --
> Dr Peter O'Toole
> Head of Imaging and Cytometry
> Technology Facility
> Department of Biology (Area 15)
> University of York
> YORK
> YO10 5DD
>
> Tel : +44 (0)1904 328722
> Fax : +44 (0)1904 328804
> email : [hidden email]
> www.york.ac.uk/biology/tf
>
> EMAIL DISCLAIMER http://www.york.ac.uk/docs/disclaimer/email.htm
>
Paul Constantinou Paul Constantinou
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Re: Mystery microscope

In reply to this post by mmodel
*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi all,

The patents for the Richardson technology were recently acquired by Quorum
Technologies in Guelph, ON.  An enhanced version of this technology (under
the name 'Riveal Contrast') will be launched at Neuroscience in San Diego,
CA this November for anyone interested.

Regards,
Paul


Paul Constantinou, Ph.D.
Imaging & Product Development Consultant

Quorum Technologies Inc.
4673 Wellington Rd. #35 RR#6
Guelph, Ontario
Canada  N1H 6J3
Tel (519) 824-0854 x 29
Fax (519) 824-5845
email [hidden email]
www.quorumtechnologies.com
Andreas Bruckbauer Andreas Bruckbauer
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Re: Mystery microscope

In reply to this post by mmodel
*****
To join, leave or search the confocal microscopy listserv, go to:
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*****

Another mystery microscope:
http://www.grayfieldoptical.com/

best wishes

Andreas

 

 


 

 

-----Original Message-----
From: MODEL, MICHAEL <[hidden email]>
To: [hidden email]
Sent: Mon, 4 Oct 2010 15:55
Subject: Re: Mystery microscope


*****

To join, leave or search the confocal microscopy listserv, go to:

http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy

*****



Thanks for all the responses from which I have already learned many new things.

I remember they were saying that it was based on some principle that had never

been published or patented. I also think (though not 100% certain) that it

worked in all the common transmission modes. But all they had was a brochure..







-----Original Message-----

From: Confocal Microscopy List [mailto:[hidden email]] On

Behalf Of Judy Trogadis

Sent: Monday, October 04, 2010 9:22 AM

To: [hidden email]

Subject: Re: Mystery microscope



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Yes, it does sound like the Richardson microscope. It has no oculars. They also

used an oil coupled condensor. Dr. Richardson sold the company and the patents

to someone else. I can try to find out who is selling them now, I know one of

their ex-employees.

Judy





Judy Trogadis

Bio-Imaging Coordinator

St. Michael's Hospital, 7Queen

30 Bond St.

Toronto, ON M5B 1W8, Canada

ph:  416-864-6060  x6337

pager: 416-685-9219

fax: 416-864-5046

[hidden email]





>>> "O'Toole, P" <[hidden email]> 10/4/2010 4:43 AM >>>

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  Was it the Richardson Technologies instrument? This appeared to

resolve unstained mitochondria through the transmitted light path.  Not

sure that it could collect a large depth of field and also thought it

was 2004 or 2006 that they were present.



Phase Focus is a different system again that can 'virtual' focus through

z once a x,y tile has been collected.  This system uses coherent light

and collects the light in the transmission light path.



Best



Pete



On 03/10/2010 17:56, MODEL, MICHAEL wrote:

> *****

> To join, leave or search the confocal microscopy listserv, go to:

> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy 

> *****

>

> Dear colleagues... (Sorry if you are getting this message second time, I am

having some trouble sending to the Confocal List)

>

> I was at the Microscience meeting in London in 2008, and in one vendor booth

they were telling (not really displaying because they didn't have it with them)

about a strange microscope that achieves a very good resolution in transmission,

better than the diffraction limit, and a large depth of field. They didn't

explain how it works. I think the company was based in Germany, but I don't

remember the name.  Does anyone know what it could have been or the company

name? I would like to contact them. Thanks!

>

> Mike Model

> Kent State University



--

Dr Peter O'Toole

Head of Imaging and Cytometry

Technology Facility

Department of Biology (Area 15)

University of York

YORK

YO10 5DD



Tel : +44 (0)1904 328722

Fax : +44 (0)1904 328804

email : [hidden email]

www.york.ac.uk/biology/tf



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mmodel mmodel
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Re: Mystery microscope

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THIS IS IT. Thank you! - Mike

________________________________________
From: Confocal Microscopy List [[hidden email]] On Behalf Of Andreas Bruckbauer [[hidden email]]
Sent: Tuesday, October 05, 2010 1:49 AM
To: [hidden email]
Subject: Re: Mystery microscope

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Another mystery microscope:
http://www.grayfieldoptical.com/

best wishes

Andreas










-----Original Message-----
From: MODEL, MICHAEL <[hidden email]>
To: [hidden email]
Sent: Mon, 4 Oct 2010 15:55
Subject: Re: Mystery microscope


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Thanks for all the responses from which I have already learned many new things.

I remember they were saying that it was based on some principle that had never

been published or patented. I also think (though not 100% certain) that it

worked in all the common transmission modes. But all they had was a brochure..







-----Original Message-----

From: Confocal Microscopy List [mailto:[hidden email]] On

Behalf Of Judy Trogadis

Sent: Monday, October 04, 2010 9:22 AM

To: [hidden email]

Subject: Re: Mystery microscope



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Yes, it does sound like the Richardson microscope. It has no oculars. They also

used an oil coupled condensor. Dr. Richardson sold the company and the patents

to someone else. I can try to find out who is selling them now, I know one of

their ex-employees.

Judy





Judy Trogadis

Bio-Imaging Coordinator

St. Michael's Hospital, 7Queen

30 Bond St.

Toronto, ON M5B 1W8, Canada

ph:  416-864-6060  x6337

pager: 416-685-9219

fax: 416-864-5046

[hidden email]





>>> "O'Toole, P" <[hidden email]> 10/4/2010 4:43 AM >>>

*****

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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy

*****



  Was it the Richardson Technologies instrument? This appeared to

resolve unstained mitochondria through the transmitted light path.  Not

sure that it could collect a large depth of field and also thought it

was 2004 or 2006 that they were present.



Phase Focus is a different system again that can 'virtual' focus through

z once a x,y tile has been collected.  This system uses coherent light

and collects the light in the transmission light path.



Best



Pete



On 03/10/2010 17:56, MODEL, MICHAEL wrote:

> *****

> To join, leave or search the confocal microscopy listserv, go to:

> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy

> *****

>

> Dear colleagues... (Sorry if you are getting this message second time, I am

having some trouble sending to the Confocal List)

>

> I was at the Microscience meeting in London in 2008, and in one vendor booth

they were telling (not really displaying because they didn't have it with them)

about a strange microscope that achieves a very good resolution in transmission,

better than the diffraction limit, and a large depth of field. They didn't

explain how it works. I think the company was based in Germany, but I don't

remember the name.  Does anyone know what it could have been or the company

name? I would like to contact them. Thanks!

>

> Mike Model

> Kent State University



--

Dr Peter O'Toole

Head of Imaging and Cytometry

Technology Facility

Department of Biology (Area 15)

University of York

YORK

YO10 5DD



Tel : +44 (0)1904 328722

Fax : +44 (0)1904 328804

email : [hidden email]

www.york.ac.uk/biology/tf



EMAIL DISCLAIMER http://www.york.ac.uk/docs/disclaimer/email.htm
Johannes Helm Johannes Helm
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Re: Mystery microscope

In reply to this post by mmodel
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Good afternoon,

there have been a number of important answers to Dr. Model's question.

I allow myself to recommend for those interested in history, also in how
developments can follow strange, perhaps personally tragic, paths, to do a
search on the internet on "Royal Raymond Rife".

I beg your pardon if this hint has been provided any earlier and thus
would be "old news".

Best wishes,

Johannes


--
P. Johannes Helm, M.Sc. PhD
Seniorengineer
CMBN
University of Oslo
Institute of Basic Medical Science
Department of Anatomy
Postboks 1105 - Blindern
NO-0317 Oslo

Voice: +47 228 51159
Fax: +47 228 51499

WWW: folk.uio.no/jhelm

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear colleagues... (Sorry if you are getting this message second time, I
> am having some trouble sending to the Confocal List)
>
> I was at the Microscience meeting in London in 2008, and in one vendor
> booth they were telling (not really displaying because they didn't have it
> with them) about a strange microscope that achieves a very good resolution
> in transmission, better than the diffraction limit, and a large depth of
> field. They didn't explain how it works. I think the company was based in
> Germany, but I don't remember the name.  Does anyone know what it could
> have been or the company name? I would like to contact them. Thanks!
>
> Mike Model
> Kent State University
>
Martin Wessendorf-2 Martin Wessendorf-2
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Re: Mystery microscope

In reply to this post by Andreas Bruckbauer
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On 10/5/2010 12:49 AM, Andreas Bruckbauer wrote:

> Another mystery microscope:
> http://www.grayfieldoptical.com/

Does anybody (--Guy?  Barbara?  Jim?) have an idea of the optical
principles underlying this instrument?  Has this been published
anywhere?

These are surprising claims (e.g. 100 nm resolution in what appears to
be a widefield microscope)...though lately, lord knows, there've been
lots of surprises to be had.

Thanks--

Martin
--
Martin Wessendorf, Ph.D.                   office: (612) 626-0145
Assoc Prof, Dept Neuroscience                 lab: (612) 624-2991
University of Minnesota             Preferred FAX: (612) 624-8118
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: (612) 626-5009
Minneapolis, MN  55455                    e-mail: [hidden email]
Steffen Dietzel Steffen Dietzel
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Re: Mystery microscope

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On 05.10.2010 16:08, Martin Wessendorf wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> On 10/5/2010 12:49 AM, Andreas Bruckbauer wrote:
>
>> Another mystery microscope:
>> http://www.grayfieldoptical.com/
>
> Does anybody (--Guy? Barbara? Jim?) have an idea of the optical
> principles underlying this instrument? Has this been published anywhere?
>
> These are surprising claims (e.g. 100 nm resolution in what appears to
> be a widefield microscope)...though lately, lord knows, there've been
> lots of surprises to be had.

 From http://www.grayfieldoptical.com/faq.html
"Although the exact working principle is a trade secret, ..."
After this, there is some hand-waving markteting talk.

I can't really comment on the images of those test slides and diatomes
on the web page, but from the images of cells they show, I am not
impressed. Doesn't look like 100 nm resolution to me. see Grayfield
homepage or
http://www.mikroskop-olbrich.de/ergonom_4000.html

There is also a movie at
http://video.google.de/videoplay?docid=839679424840450517#

Microscopy starts at ~4:10. This movie apparently also explains the real
cause of cancer, I didn't watch all of it, though, it's 48 minutes.
More videos at
http://www.grayfieldoptical.com/online_videos.html

Other than that, the "Institut f. Interdisziplinäre Grundlagenforschung
Kurt Olbrich" can be googled as a reference for confirming the effect of
various "water vitalising" procedures on German web pages.

http://www.boehm-egger.de/produkte_prufungen.htm
http://www.theta-reiki.de/trinkwasser/die-loesung/
http://www.aquavitera.de/wasserbelebung-pejosan-wasservitalisierung-schauberger-wasserwirbler-aquavitera-wasserveredler/wasserveredler-pejosan-petit-.119.155.de.html

The subject of this thread seems to be quite fitting, in retrospect.

Steffen
Guy Cox-2 Guy Cox-2
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Re: Mystery microscope

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OK, since I was asked to comment.  Nothing in this makes sense.  Why
should the 100nm resolution version cost so much more?  Maybe just so
that people don't buy it and so won't find out that it doesn't work?

There are several 100nm (or better) optical resolution microscopes you
can buy based on well published and dependable principles.  (Structured
illumination, STED, 4pi, stochastic single molecule imaging.)

There are also microscopes with extended depth of field (but no lateral
super-resolution).  Carol Cogswell was among those working in this
field.

                                          Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
     http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
Madsen Building F09, University of Sydney, NSW 2006

Phone +61 2 9351 3176     Fax +61 2 9351 7682
             Mobile 0413 281 861
______________________________________________
      http://www.guycox.net
 


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Martin Wessendorf
Sent: Wednesday, 6 October 2010 1:08 AM
To: [hidden email]
Subject: Re: Mystery microscope

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On 10/5/2010 12:49 AM, Andreas Bruckbauer wrote:

> Another mystery microscope:
> http://www.grayfieldoptical.com/

Does anybody (--Guy?  Barbara?  Jim?) have an idea of the optical
principles underlying this instrument?  Has this been published
anywhere?

These are surprising claims (e.g. 100 nm resolution in what appears to
be a widefield microscope)...though lately, lord knows, there've been
lots of surprises to be had.

Thanks--

Martin
--
Martin Wessendorf, Ph.D.                   office: (612) 626-0145
Assoc Prof, Dept Neuroscience                 lab: (612) 624-2991
University of Minnesota             Preferred FAX: (612) 624-8118
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: (612) 626-5009
Minneapolis, MN  55455                    e-mail: [hidden email]

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05:35:00
Tobias Baskin Tobias Baskin
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Small molecules in the endoplasmic reticulum

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Hi microscopists,
                Anyone know of a way to load a small (say less than 1
kDa) fluorescent molecule into the ER lumen? A soluble molecule
rather than a membrane probe. A small amount of background in cytosol
or other organelles could be managed.

        Many thanks,
                Tobias Baskin
--
       _      ____          __   ____
      /  \   /          / \    /   \ \        Tobias I. Baskin
     /   /  /          /   \   \      \         Biology Department
    /_ /   __      /__ \   \       \__    611 N. Pleasant St.
   /      /          /       \   \       \        University of Massachusetts
  /      /          /         \   \       \    Amherst, MA, 01003
/      / ___   /           \   \__/  \ ____
www.bio.umass.edu/biology/baskin
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243
Gens, John Scott Gens, John Scott
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Re: Small molecules in the endoplasmic reticulum

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Tobias-

I assume you are still working with plant cells?  I recall that Hartmut
Quader showed some pretty nice movies of plant ER dynamics using DiOC6
(which I know, doesn't meet your non-membrane probe criteria). I seem
to recall hearing that the same method doesn't necessarily work in
animal cells.

If I might ask, what is your rationale for needing a label specifically
for the lumen of the ER?

Cheers,

Scott
--
J. Scott Gens, Ph.D.
Biocomplexity Institute
Indiana University Physics
Office:     Room 047H, Simon Interdisciplinary Sciences Bldg.
Multi-photon Confocal: 063 Swain Hall West.
Bloomington IN 47405
812-855-7946
[hidden email]



Quoting Tobias Baskin <[hidden email]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi microscopists,
> Anyone know of a way to load a small (say less than 1 kDa)
> fluorescent molecule into the ER lumen? A soluble molecule rather
> than a membrane probe. A small amount of background in cytosol or
> other organelles could be managed.
>
> Many thanks,
> Tobias Baskin
> --
>       _      ____          __   ____
>      /  \   /          / \    /   \ \        Tobias I. Baskin
>     /   /  /          /   \   \      \         Biology Department
>    /_ /   __      /__ \   \       \__    611 N. Pleasant St.
>   /      /          /       \   \       \        University of Massachusetts
>  /      /          /         \   \       \    Amherst, MA, 01003
> /      / ___   /           \   \__/  \ ____
> www.bio.umass.edu/biology/baskin
> Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243
>
Rosemary.White Rosemary.White
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Re: Small molecules in the endoplasmic reticulum

In reply to this post by Tobias Baskin
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Hi Tobias,

Dave Collings [hidden email], Debbie Barton
[hidden email] and others in Robyn's group have been trying
just this for a while.  They may not be on this list, they've just submitted
a ms. on cell-cell transport via the ER lumen, but not sure exactly what
they used or how they got it into the ER except by microinjection.

cheers,
Rosemary


On 7/10/10 7:33 AM, "Tobias Baskin" <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi microscopists,
> Anyone know of a way to load a small (say less than 1
> kDa) fluorescent molecule into the ER lumen? A soluble molecule
> rather than a membrane probe. A small amount of background in cytosol
> or other organelles could be managed.
>
> Many thanks,
> Tobias Baskin
12