Mystery microscope
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Michael Schell |
Re: Small molecules in the endoplasmic reticulum
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** How about Mag-fura2, which can be loaded as an AM ester and used to detect changes in luminal Ca2+? For example: Hofer AM et al., EMBO J. 17:1986 (1998). Michael J. Schell, Ph.D., CIV, USUHS Assist. Professor Dept. of Pharmacology Uniformed Services University 4301 Jones Bridge Rd. Bethesda, MD 20814-3220 tel: (301) 295-3249 [hidden email] >>> Tobias Baskin <[hidden email]> 10/06/10 4:36 PM >>> ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi microscopists, Anyone know of a way to load a small (say less than 1 kDa) fluorescent molecule into the ER lumen? A soluble molecule rather than a membrane probe. A small amount of background in cytosol or other organelles could be managed. Many thanks, Tobias Baskin - Classification: UNCLASSIFIED Caveats: None Classification: UNCLASSIFIED Caveats: None |
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In reply to this post by Guy Cox-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I agree, seems to be some pseudoscience, the image of the Richardson test slide on their webpage shows that the resolution is somewhere between 260 - 300 nm. Andreas -----Original Message----- From: Guy Cox <[hidden email]> To: [hidden email] Sent: Wed, 6 Oct 2010 17:11 Subject: Re: Mystery microscope ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** OK, since I was asked to comment. Nothing in this makes sense. Why should the 100nm resolution version cost so much more? Maybe just so that people don't buy it and so won't find out that it doesn't work? There are several 100nm (or better) optical resolution microscopes you can buy based on well published and dependable principles. (Structured illumination, STED, 4pi, stochastic single molecule imaging.) There are also microscopes with extended depth of field (but no lateral super-resolution). Carol Cogswell was among those working in this field. Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Australian Centre for Microscopy & Microanalysis, Madsen Building F09, University of Sydney, NSW 2006 Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Martin Wessendorf Sent: Wednesday, 6 October 2010 1:08 AM To: [hidden email] Subject: Re: Mystery microscope ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** On 10/5/2010 12:49 AM, Andreas Bruckbauer wrote: > Another mystery microscope: > http://www.grayfieldoptical.com/ Does anybody (--Guy? Barbara? Jim?) have an idea of the optical principles underlying this instrument? Has this been published anywhere? These are surprising claims (e.g. 100 nm resolution in what appears to be a widefield microscope)...though lately, lord knows, there've been lots of surprises to be had. Thanks-- Martin -- Martin Wessendorf, Ph.D. office: (612) 626-0145 Assoc Prof, Dept Neuroscience lab: (612) 624-2991 University of Minnesota Preferred FAX: (612) 624-8118 6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009 Minneapolis, MN 55455 e-mail: [hidden email] No virus found in this incoming message. Checked by AVG - www.avg.com Version: 9.0.862 / Virus Database: 271.1.1/3165 - Release Date: 10/05/10 05:35:00 |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** HI All Here is some more information about the mystery microscope, all seems a bit strange to me but mildly entertaining too! http://www.rife.de/index.html Thanks Robert Robert Fernandez Bioimaging Facility University of Manchester UK -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Andreas Bruckbauer Sent: 07 October 2010 11:30 To: [hidden email] Subject: Re: Mystery microscope ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I agree, seems to be some pseudoscience, the image of the Richardson test slide on their webpage shows that the resolution is somewhere between 260 - 300 nm. Andreas -----Original Message----- From: Guy Cox <[hidden email]> To: [hidden email] Sent: Wed, 6 Oct 2010 17:11 Subject: Re: Mystery microscope ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** OK, since I was asked to comment. Nothing in this makes sense. Why should the 100nm resolution version cost so much more? Maybe just so that people don't buy it and so won't find out that it doesn't work? There are several 100nm (or better) optical resolution microscopes you can buy based on well published and dependable principles. (Structured illumination, STED, 4pi, stochastic single molecule imaging.) There are also microscopes with extended depth of field (but no lateral super-resolution). Carol Cogswell was among those working in this field. Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Australian Centre for Microscopy & Microanalysis, Madsen Building F09, University of Sydney, NSW 2006 Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Martin Wessendorf Sent: Wednesday, 6 October 2010 1:08 AM To: [hidden email] Subject: Re: Mystery microscope ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** On 10/5/2010 12:49 AM, Andreas Bruckbauer wrote: > Another mystery microscope: > http://www.grayfieldoptical.com/ Does anybody (--Guy? Barbara? Jim?) have an idea of the optical principles underlying this instrument? Has this been published anywhere? These are surprising claims (e.g. 100 nm resolution in what appears to be a widefield microscope)...though lately, lord knows, there've been lots of surprises to be had. Thanks-- Martin -- Martin Wessendorf, Ph.D. office: (612) 626-0145 Assoc Prof, Dept Neuroscience lab: (612) 624-2991 University of Minnesota Preferred FAX: (612) 624-8118 6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009 Minneapolis, MN 55455 e-mail: [hidden email] No virus found in this incoming message. Checked by AVG - www.avg.com Version: 9.0.862 / Virus Database: 271.1.1/3165 - Release Date: 10/05/10 05:35:00 |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Entertaining, and seriously quack, but I don't see any claims of 100nm resolution. Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Australian Centre for Microscopy & Microanalysis, Madsen Building F09, University of Sydney, NSW 2006 Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Robert Fernandez Sent: Thursday, 7 October 2010 11:49 PM To: [hidden email] Subject: Re: Mystery microscope ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** HI All Here is some more information about the mystery microscope, all seems a bit strange to me but mildly entertaining too! http://www.rife.de/index.html Thanks Robert Robert Fernandez Bioimaging Facility University of Manchester UK -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Andreas Bruckbauer Sent: 07 October 2010 11:30 To: [hidden email] Subject: Re: Mystery microscope ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I agree, seems to be some pseudoscience, the image of the Richardson test slide on their webpage shows that the resolution is somewhere between 260 - 300 nm. Andreas -----Original Message----- From: Guy Cox <[hidden email]> To: [hidden email] Sent: Wed, 6 Oct 2010 17:11 Subject: Re: Mystery microscope ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** OK, since I was asked to comment. Nothing in this makes sense. Why should the 100nm resolution version cost so much more? Maybe just so that people don't buy it and so won't find out that it doesn't work? There are several 100nm (or better) optical resolution microscopes you can buy based on well published and dependable principles. (Structured illumination, STED, 4pi, stochastic single molecule imaging.) There are also microscopes with extended depth of field (but no lateral super-resolution). Carol Cogswell was among those working in this field. Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Australian Centre for Microscopy & Microanalysis, Madsen Building F09, University of Sydney, NSW 2006 Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Martin Wessendorf Sent: Wednesday, 6 October 2010 1:08 AM To: [hidden email] Subject: Re: Mystery microscope ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** On 10/5/2010 12:49 AM, Andreas Bruckbauer wrote: > Another mystery microscope: > http://www.grayfieldoptical.com/ Does anybody (--Guy? Barbara? Jim?) have an idea of the optical principles underlying this instrument? Has this been published anywhere? These are surprising claims (e.g. 100 nm resolution in what appears to be a widefield microscope)...though lately, lord knows, there've been lots of surprises to be had. Thanks-- Martin -- Martin Wessendorf, Ph.D. office: (612) 626-0145 Assoc Prof, Dept Neuroscience lab: (612) 624-2991 University of Minnesota Preferred FAX: (612) 624-8118 6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009 Minneapolis, MN 55455 e-mail: [hidden email] No virus found in this incoming message. Checked by AVG - www.avg.com Version: 9.0.862 / Virus Database: 271.1.1/3165 - Release Date: 10/05/10 05:35:00 No virus found in this incoming message. Checked by AVG - www.avg.com Version: 9.0.862 / Virus Database: 271.1.1/3165 - Release Date: 10/07/10 05:34:00 |
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In reply to this post by Robert Fernandez
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear Confocal Listers, I suggest that everyone try to get a demo of it in your labs, it would be interesting if they even would do such a thing. But then and only then can you truly know for sure whether it is a load of crock or not. As the old adage goes: Seeing is believing... Pete P.S. If they start to get protective of this technology and won't let you demo it, then you know that they are the swindlers that they seem to be. On Oct 7, 2010, at 14:49 PM, Robert Fernandez wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > HI All > > Here is some more information about the mystery microscope, all seems a bit strange to me but mildly entertaining too! > > http://www.rife.de/index.html > > Thanks > > Robert > > Robert Fernandez > Bioimaging Facility > University of Manchester > UK > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Andreas Bruckbauer > Sent: 07 October 2010 11:30 > To: [hidden email] > Subject: Re: Mystery microscope > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > > I agree, seems to be some pseudoscience, the image of the Richardson test slide on their webpage shows that the resolution is somewhere between 260 - 300 nm. > > Andreas > > > > > > > > -----Original Message----- > From: Guy Cox <[hidden email]> > To: [hidden email] > Sent: Wed, 6 Oct 2010 17:11 > Subject: Re: Mystery microscope > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > OK, since I was asked to comment. Nothing in this makes sense. Why > > should the 100nm resolution version cost so much more? Maybe just so > > that people don't buy it and so won't find out that it doesn't work? > > > > There are several 100nm (or better) optical resolution microscopes you > > can buy based on well published and dependable principles. (Structured > > illumination, STED, 4pi, stochastic single molecule imaging.) > > > > There are also microscopes with extended depth of field (but no lateral > > super-resolution). Carol Cogswell was among those working in this > > field. > > > > Guy > > > > Optical Imaging Techniques in Cell Biology > > by Guy Cox CRC Press / Taylor & Francis > > http://www.guycox.com/optical.htm > > ______________________________________________ > > Associate Professor Guy Cox, MA, DPhil(Oxon) > > Australian Centre for Microscopy & Microanalysis, > > Madsen Building F09, University of Sydney, NSW 2006 > > > > Phone +61 2 9351 3176 Fax +61 2 9351 7682 > > Mobile 0413 281 861 > > ______________________________________________ > > http://www.guycox.net > > > > > > > > -----Original Message----- > > From: Confocal Microscopy List [mailto:[hidden email]] > > On Behalf Of Martin Wessendorf > > Sent: Wednesday, 6 October 2010 1:08 AM > > To: [hidden email] > > Subject: Re: Mystery microscope > > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > On 10/5/2010 12:49 AM, Andreas Bruckbauer wrote: > > > >> Another mystery microscope: > >> http://www.grayfieldoptical.com/ > > > > Does anybody (--Guy? Barbara? Jim?) have an idea of the optical > > principles underlying this instrument? Has this been published > > anywhere? > > > > These are surprising claims (e.g. 100 nm resolution in what appears to > > be a widefield microscope)...though lately, lord knows, there've been > > lots of surprises to be had. > > > > Thanks-- > > > > Martin > > -- > > Martin Wessendorf, Ph.D. office: (612) 626-0145 > > Assoc Prof, Dept Neuroscience lab: (612) 624-2991 > > University of Minnesota Preferred FAX: (612) 624-8118 > > 6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009 > > Minneapolis, MN 55455 e-mail: [hidden email] > > > > No virus found in this incoming message. > > Checked by AVG - www.avg.com > > Version: 9.0.862 / Virus Database: 271.1.1/3165 - Release Date: 10/05/10 > > 05:35:00 > > > |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** So far they haven't replied to my email and a phone message.. - Mike -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Peter Pitrone Sent: Thursday, October 07, 2010 11:49 AM To: [hidden email] Subject: Re: Mystery microscope ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear Confocal Listers, I suggest that everyone try to get a demo of it in your labs, it would be interesting if they even would do such a thing. But then and only then can you truly know for sure whether it is a load of crock or not. As the old adage goes: Seeing is believing... Pete P.S. If they start to get protective of this technology and won't let you demo it, then you know that they are the swindlers that they seem to be. On Oct 7, 2010, at 14:49 PM, Robert Fernandez wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > HI All > > Here is some more information about the mystery microscope, all seems a bit strange to me but mildly entertaining too! > > http://www.rife.de/index.html > > Thanks > > Robert > > Robert Fernandez > Bioimaging Facility > University of Manchester > UK > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Andreas Bruckbauer > Sent: 07 October 2010 11:30 > To: [hidden email] > Subject: Re: Mystery microscope > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > > I agree, seems to be some pseudoscience, the image of the Richardson test slide on their webpage shows that the resolution is somewhere between 260 - 300 nm. > > Andreas > > > > > > > > -----Original Message----- > From: Guy Cox <[hidden email]> > To: [hidden email] > Sent: Wed, 6 Oct 2010 17:11 > Subject: Re: Mystery microscope > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > OK, since I was asked to comment. Nothing in this makes sense. Why > > should the 100nm resolution version cost so much more? Maybe just so > > that people don't buy it and so won't find out that it doesn't work? > > > > There are several 100nm (or better) optical resolution microscopes you > > can buy based on well published and dependable principles. (Structured > > illumination, STED, 4pi, stochastic single molecule imaging.) > > > > There are also microscopes with extended depth of field (but no lateral > > super-resolution). Carol Cogswell was among those working in this > > field. > > > > Guy > > > > Optical Imaging Techniques in Cell Biology > > by Guy Cox CRC Press / Taylor & Francis > > http://www.guycox.com/optical.htm > > ______________________________________________ > > Associate Professor Guy Cox, MA, DPhil(Oxon) > > Australian Centre for Microscopy & Microanalysis, > > Madsen Building F09, University of Sydney, NSW 2006 > > > > Phone +61 2 9351 3176 Fax +61 2 9351 7682 > > Mobile 0413 281 861 > > ______________________________________________ > > http://www.guycox.net > > > > > > > > -----Original Message----- > > From: Confocal Microscopy List [mailto:[hidden email]] > > On Behalf Of Martin Wessendorf > > Sent: Wednesday, 6 October 2010 1:08 AM > > To: [hidden email] > > Subject: Re: Mystery microscope > > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > On 10/5/2010 12:49 AM, Andreas Bruckbauer wrote: > > > >> Another mystery microscope: > >> http://www.grayfieldoptical.com/ > > > > Does anybody (--Guy? Barbara? Jim?) have an idea of the optical > > principles underlying this instrument? Has this been published > > anywhere? > > > > These are surprising claims (e.g. 100 nm resolution in what appears to > > be a widefield microscope)...though lately, lord knows, there've been > > lots of surprises to be had. > > > > Thanks-- > > > > Martin > > -- > > Martin Wessendorf, Ph.D. office: (612) 626-0145 > > Assoc Prof, Dept Neuroscience lab: (612) 624-2991 > > University of Minnesota Preferred FAX: (612) 624-8118 > > 6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009 > > Minneapolis, MN 55455 e-mail: [hidden email] > > > > No virus found in this incoming message. > > Checked by AVG - www.avg.com > > Version: 9.0.862 / Virus Database: 271.1.1/3165 - Release Date: 10/05/10 > > 05:35:00 > > > |
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