NIR excitation light source

[Judy Trogadis-2]

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Fellow (confocal) microscopists,

Excluding lasers, does anyone know of a light source that would emit in NIR (around 800nm) wavelength with a good quantum efficiency? Are there other benchmarks to measure signal power?

Thank you

Judy

Judy Trogadis
Bio-Imaging Coordinator
Keenan Research Centre, St. Michael's
209 Victoria Street
Toronto M5B 1T8, Canada
office: 416-864-6060 ext. 77612
imaging facility:           ext. 77434
cell:        416-909-9878
[hidden email]<mailto:[hidden email]>

[Martin Wessendorf-2]

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Dear Judy--

On 3/19/2012 12:30 PM, Judy Trogadis wrote:

> Excluding lasers, does anyone know of a light source that would emit in NIR (around 800nm) wavelength with a good quantum efficiency? Are there other benchmarks to measure signal power?

A conventional incandescent bulb is pretty good--I've used one for NIR
DIC--but it really depends upon how much power you need.

The following link (no commercial connection) lists NIR LEDs in the 800
nm range; no idea of cost:  http://www.roithner-laser.com/led_diverse.html

Good luck!

Martin
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]

[Pascal Weber]

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Try this

http://www.thorlabs.com/NewGroupPage9.cfm?ObjectGroup_ID=2615

[Judy Trogadis-2]

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Hi, Pascal,
These look amazingly practical. Too good to be true?
Will be talking to Thorlabs.
Thank you!
Judy


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Pascal Weber
Sent: Monday, March 19, 2012 4:15 PM
To: [hidden email]
Subject: Re: NIR excitation light source

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Try this

http://www.thorlabs.com/NewGroupPage9.cfm?ObjectGroup_ID=2615

[Judy Trogadis-2]

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Hello, Martin

In addition to having a drawer of filter cubes, we will now have a drawer of LED's. Not a bad solution. Thank you, the prices are excellent but I need an electronics expert because these are just the raw components.
Perhaps my nephew in his basement . . . . . . .

Judy


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Martin Wessendorf
Sent: Monday, March 19, 2012 1:46 PM
To: [hidden email]
Subject: Re: NIR excitation light source

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Dear Judy--

On 3/19/2012 12:30 PM, Judy Trogadis wrote:

> Excluding lasers, does anyone know of a light source that would emit in NIR (around 800nm) wavelength with a good quantum efficiency? Are there other benchmarks to measure signal power?

A conventional incandescent bulb is pretty good--I've used one for NIR
DIC--but it really depends upon how much power you need.

The following link (no commercial connection) lists NIR LEDs in the 800
nm range; no idea of cost:  http://www.roithner-laser.com/led_diverse.html

Good luck!

Martin
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]

[Craig Brideau]

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To drive an LED you need a 'constant current' supply.  You can make simple
ones with a small part called an LM317, but if you aren't up to electronics
hacking then I'd recommend getting one of Thor's T-Cube drivers.  The
LEDD1B is just under $300 USD and will drive an LED up to 1200mA.  It's not
a bad deal and it should already be wired for the connectors of Thor's own
LED products.  I think it would save you a lot of time and headache.

http://www.thorlabs.com/NewGroupPage9.cfm?ObjectGroup_ID=2616

Craig

On Mon, Mar 19, 2012 at 2:40 PM, Judy Trogadis <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello, Martin
>
> In addition to having a drawer of filter cubes, we will now have a drawer
> of LED's. Not a bad solution. Thank you, the prices are excellent but I
> need an electronics expert because these are just the raw components.
> Perhaps my nephew in his basement . . . . . . .
>
> Judy
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Martin Wessendorf
> Sent: Monday, March 19, 2012 1:46 PM
> To: [hidden email]
> Subject: Re: NIR excitation light source
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear Judy--
>
> On 3/19/2012 12:30 PM, Judy Trogadis wrote:
>
> > Excluding lasers, does anyone know of a light source that would emit in
> NIR (around 800nm) wavelength with a good quantum efficiency? Are there
> other benchmarks to measure signal power?
>
> A conventional incandescent bulb is pretty good--I've used one for NIR
> DIC--but it really depends upon how much power you need.
>
> The following link (no commercial connection) lists NIR LEDs in the 800
> nm range; no idea of cost:  http://www.roithner-laser.com/led_diverse.html
>
> Good luck!
>
> Martin
> --
> Martin Wessendorf, Ph.D.                   office: [phone]
> Assoc Prof, Dept Neuroscience                 lab: [phone]
> University of Minnesota             Preferred FAX: [phone]
> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
> Minneapolis, MN  55455                    e-mail: [hidden email]
>

[Pascal Weber]

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I have some questions for you. What do you do exactly. Which microscope would
you adapt your installation? We found on the internet diodes more powerful than
10 watts.

[Judy Trogadis-2]

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Whole animal imagers often use NIR dyes for a variety of different reasons. After processing thin sections for histology, we would like to image those same areas. From the Thorlabs website, it seems as if we can simply exchange the light guide in the epillumination port on one of our microscopes (acceptable models are also listed) with the LED's attachment and it's just a matter of matching the light with the appropriate filter cube.

I think 10 watts would provide enough light, don't you think?

Judy


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Pascal Weber
Sent: Monday, March 19, 2012 5:19 PM
To: [hidden email]
Subject: Re: NIR excitation light source

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I have some questions for you. What do you do exactly. Which microscope would
you adapt your installation? We found on the internet diodes more powerful than
10 watts.

[Judy Trogadis-2]

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You're the second person who has recommended Thorlabs. You guys know it all!
Thank you!
Judy


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Craig Brideau
Sent: Monday, March 19, 2012 4:57 PM
To: [hidden email]
Subject: Re: NIR excitation light source

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To drive an LED you need a 'constant current' supply.  You can make simple
ones with a small part called an LM317, but if you aren't up to electronics
hacking then I'd recommend getting one of Thor's T-Cube drivers.  The
LEDD1B is just under $300 USD and will drive an LED up to 1200mA.  It's not
a bad deal and it should already be wired for the connectors of Thor's own
LED products.  I think it would save you a lot of time and headache.

http://www.thorlabs.com/NewGroupPage9.cfm?ObjectGroup_ID=2616

Craig

On Mon, Mar 19, 2012 at 2:40 PM, Judy Trogadis <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello, Martin
>
> In addition to having a drawer of filter cubes, we will now have a drawer
> of LED's. Not a bad solution. Thank you, the prices are excellent but I
> need an electronics expert because these are just the raw components.
> Perhaps my nephew in his basement . . . . . . .
>
> Judy
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Martin Wessendorf
> Sent: Monday, March 19, 2012 1:46 PM
> To: [hidden email]
> Subject: Re: NIR excitation light source
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear Judy--
>
> On 3/19/2012 12:30 PM, Judy Trogadis wrote:
>
> > Excluding lasers, does anyone know of a light source that would emit in
> NIR (around 800nm) wavelength with a good quantum efficiency? Are there
> other benchmarks to measure signal power?
>
> A conventional incandescent bulb is pretty good--I've used one for NIR
> DIC--but it really depends upon how much power you need.
>
> The following link (no commercial connection) lists NIR LEDs in the 800
> nm range; no idea of cost:  http://www.roithner-laser.com/led_diverse.html
>
> Good luck!
>
> Martin
> --
> Martin Wessendorf, Ph.D.                   office: [phone]
> Assoc Prof, Dept Neuroscience                 lab: [phone]
> University of Minnesota             Preferred FAX: [phone]
> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
> Minneapolis, MN  55455                    e-mail: [hidden email]
>

[Mike Buchin]

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We have had good luck with Prizmatix www.prizmatix.com high power LED'.s
They only show product out to 630 nm but I am sure they could provide NIR
(730, 850 etc.).  Their drivers are fast (several microseconds) switching
and high bandwidth (up to 30 kHz rep rates).  Direct or fiber coupled to all
scope configurations.

No commercial interest.

Mike

Michael Buchin
Stanford Photonics, Inc.
Ph: 650-969-5991


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Pascal Weber
Sent: Monday, March 19, 2012 2:19 PM
To: [hidden email]
Subject: Re: NIR excitation light source

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I have some questions for you. What do you do exactly. Which microscope
would you adapt your installation? We found on the internet diodes more
powerful than
10 watts.

[Pascal Weber]

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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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Do you really think that these fluorophores emit around 800nm &#8203;?
It is always possible to adapt the cubes. Will see in SEMROCK.

http://www.semrock.com/filters.aspx

Ten watts should be enough but yo need to do electronics. are you a specialist ?

[Judy Trogadis-2]

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Yes, they do - one in particular - ICG (indocyanine green) and filter companies sell cubes specifically for that dye. Emission around 810nm.
Judy


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Pascal Weber
Sent: Monday, March 19, 2012 5:40 PM
To: [hidden email]
Subject: Re: NIR excitation light source

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Do you really think that these fluorophores emit around 800nm &#8203;?
It is always possible to adapt the cubes. Will see in SEMROCK.

http://www.semrock.com/filters.aspx

Ten watts should be enough but yo need to do electronics. are you a specialist ?

[Pascal Weber]

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The excitation wavelengths are 660 and 780 nm.
It is not exactly the same.
You will find the dichroic and emission filter at Semrock or Chroma.
To measure the power I use a mirror purchased from Thorlabs and compares
two wavelengths from the visible and the one you just installed.

[Pascal Weber]

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See that

http://www.alibaba.com/product-
gs/493247250/660nm_10w_red_led_professional_manufacturer_.html

It's a beginning.............

[Craig Brideau]

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Heh, yes, but just make sure you properly heat sink it!  Otherwise you will
have brilliant light for about a minute, then it will go from being an LED
to a DED...

Craig


On Mon, Mar 19, 2012 at 4:13 PM, Pascal Weber <[hidden email]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> See that
>
> http://www.alibaba.com/product-
> gs/493247250/660nm_10w_red_led_professional_manufacturer_.html
>
> It's a beginning.............
>