Need your help for educational poster on Super Resolution

[Barbara Foster]

*****
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*****

Dear Listers,

As follow-up to an article I wrote in 2011
(“Superresolution: Reality or a “STORM” in a
Teacup”, BioPhotonics, Jan 2011 - PDF available
at http://microscopyeducation.com/the-library/),
I’m working with a colleague to create an
educational poster on Super Resolution. Can you help with the following:
1.     Did we miss any of the current “Key” techniques? (see list below)
2.     Do you have a clear but simple explanatory
diagram (PPT slide) of any of these techniques that you can share?
3.     Do you have a gorgeous scientific image
that represents that technique?  (especially
valuable: comparison images that show widefield
fluorescence/confocal/SR or something similar)
4.     Historically, what are the key milestones
(ex: 1873/Abbe Diffraction limit; 2004/STED
becomes commercially available; 2006/PALM & STORM; 2014/Nobel Prize etc.)

We will, of course, cite credits for all
contributed materials.  Also, please remember
that this will be just a poster.  Brevity will be important.

Our target date: April (but we need to finish
work this month to hit production deadlines)

The poster will be available for FREE (Details
will follow as we get closer to the print date)

Proposed techniques to be covered:
·         Nearfield microscopy
·         RESOLFT/STED/GSD
·         SIM
·         PALM/STORM
           Video-rate, single-molecule localization microscopy

Thanks, in advance for any help you can provide!
Barbara Foster, President & Chief Consultant
Microscopy/Microscopy Education  ... "Education, not Training"
7101 Royal Glen Trail, Suite A  - McKinney, TX 75070 - P: 972-924-5310
www.MicroscopyEducation.com

Microscopy/Microscopy Education is a division of
The Microscopy & Imaging Place, Inc.

[Patrick Van Oostveldt]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Is expansion microscopy in combination with selective plane illumination not an upcomming technique that also has superresolution performance?
see:
Expansion microscopy
Fei Chen1,*, Paul W. Tillberg2,*, Edward S. Boyden1,3,4,5,6,†
+ Author Affiliations
↵†Corresponding author. E-mail: [hidden email]
↵* These authors contributed equally to this work.
Science  30 Jan 2015:
Vol. 347, Issue 6221, pp. 543-548
DOI: 10.1126/science.

The fact that we increase resolution by specific swelling of the subject and this in combination with a library of specific antibodies certainly can create breakthroughs in e.g. neuroscience.

Patrick Van Oostveldt
Sint-Denijslaan 199
9000 GENT

Mobile [phone]
Sent from my iPad

> On 4 Jan 2017, at 17:43, Barbara Foster <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Listers,
>
> As follow-up to an article I wrote in 2011 ("Superresolution: Reality or a "STORM" in a Teacup", BioPhotonics, Jan 2011 - PDF available at http://microscopyeducation.com/the-library/), I'm working with a colleague to create an educational poster on Super Resolution. Can you help with the following:
> 1.     Did we miss any of the current "Key" techniques? (see list below)
> 2.     Do you have a clear but simple explanatory diagram (PPT slide) of any of these techniques that you can share?
> 3.     Do you have a gorgeous scientific image that represents that technique?  (especially valuable: comparison images that show widefield fluorescence/confocal/SR or something similar)
> 4.     Historically, what are the key milestones (ex: 1873/Abbe Diffraction limit; 2004/STED becomes commercially available; 2006/PALM & STORM; 2014/Nobel Prize etc.)
>
> We will, of course, cite credits for all contributed materials.  Also, please remember that this will be just a poster.  Brevity will be important.
>
> Our target date: April (but we need to finish work this month to hit production deadlines)
>
> The poster will be available for FREE (Details will follow as we get closer to the print date)
>
> Proposed techniques to be covered:
> ·         Nearfield microscopy
> ·         RESOLFT/STED/GSD
> ·         SIM
> ·         PALM/STORM
>          Video-rate, single-molecule localization microscopy
>
> Thanks, in advance for any help you can provide!
> Barbara Foster, President & Chief Consultant
> Microscopy/Microscopy Education  ... "Education, not Training"
> 7101 Royal Glen Trail, Suite A  - McKinney, TX 75070 - P: 972-924-5310
> www.MicroscopyEducation.com
>
> Microscopy/Microscopy Education is a division of The Microscopy & Imaging Place, Inc.

[George McNamara]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Barbara,

Patrick v.L. already replied about Expansion Microscopy.

More items:

* "there's a fluorescent protein biosensor for that, and that, and that,
and that too" ... http://www.geomcnamara.com/fluorescent-biosensors

* current FPs are 3x brighter than EGFP (which is soooooo 1996), and can
be multimerized to make even brighter, fairly current table is at
http://www.geomcnamara.com/fluorescent-proteins-photophysics-data (some
month my web master and I will get around to adding mScarlet and other
new FPs).

* CRISPR/Cas9 (and homologs/orthologs) are hot. TALE-Lights
(https://www.ncbi.nlm.nih.gov/pubmed/24556431), CRISPRrainbow etc ...
see fig 4A of http://jcb.rupress.org/content/jcb/135/6/1685.full.pdf 
for starting point on this.

* CyTOF imaging ... Garry Nolan / Michael Angelo and Bodenmiller labs
(and check with Garry on "CODIS")

* (brand new); high resolution (1.4 um laser spot size) atmospheric
pressure MALDI MS imaging https://www.ncbi.nlm.nih.gov/pubmed/27842060

* Xiaowei Zhuang (MERFISH), as well as Long Cai, and other labs,
multiplex single cell RNAseq ... various ways to "error correct".

* single molecule RNA FISHing can be very photogenic (even without
AUCG's sequence info) ... ask Ron Cook for copyright permission to
image(s) from http://stellarisfish.smugmug.com/

* you might find more topics from titles of talks and posters from QBI
2017,

        http://www.quantitativebioimaging.com/apps/schedule (days at top
right)

http://www.quantitativebioimaging.com/apps/schedule/posters.php

* (not new - so awesome doesn't matter) You are welcome to include the
best ever example of digital slide imaging: Tiki_Goddess
http://home.earthlink.net/~tiki_goddess/TikiGoddess.jpg

* microscopy as Q.C. for single cell NGS, for example the imaging
station component of
http://www.wafergen.com/products/icell8-single-cell-system  ... Nicholas
Navin at MD Anderson Cancer Center now has single cell RNAseq at
~$1/cell with this platform (not including data analysis or data storage).

enjoy,

George


On 1/4/2017 10:43 AM, Barbara Foster wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Listers,
>
> As follow-up to an article I wrote in 2011 (“Superresolution: Reality
> or a “STORM” in a Teacup”, BioPhotonics, Jan 2011 - PDF available at
> http://microscopyeducation.com/the-library/), I’m working with a
> colleague to create an educational poster on Super Resolution. Can you
> help with the following:
> 1.     Did we miss any of the current “Key” techniques? (see list below)
> 2.     Do you have a clear but simple explanatory diagram (PPT slide)
> of any of these techniques that you can share?
> 3.     Do you have a gorgeous scientific image that represents that
> technique?  (especially valuable: comparison images that show
> widefield fluorescence/confocal/SR or something similar)
> 4.     Historically, what are the key milestones (ex: 1873/Abbe
> Diffraction limit; 2004/STED becomes commercially available; 2006/PALM
> & STORM; 2014/Nobel Prize etc.)
>
> We will, of course, cite credits for all contributed materials. Also,
> please remember that this will be just a poster.  Brevity will be
> important.
>
> Our target date: April (but we need to finish work this month to hit
> production deadlines)
>
> The poster will be available for FREE (Details will follow as we get
> closer to the print date)
>
> Proposed techniques to be covered:
> ·         Nearfield microscopy
> ·         RESOLFT/STED/GSD
> ·         SIM
> ·         PALM/STORM
>           Video-rate, single-molecule localization microscopy
>
> Thanks, in advance for any help you can provide!
> Barbara Foster, President & Chief Consultant
> Microscopy/Microscopy Education  ... "Education, not Training"
> 7101 Royal Glen Trail, Suite A  - McKinney, TX 75070 - P: 972-924-5310
> www.MicroscopyEducation.com
>
> Microscopy/Microscopy Education is a division of The Microscopy &
> Imaging Place, Inc.

--


George McNamara, PhD
Houston, TX 77054
[hidden email]
https://www.linkedin.com/in/georgemcnamara
https://works.bepress.com/gmcnamara/75/
http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650

[Barbara Foster]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Thanks for all of this.

-Barbara

At 04:44 AM 1/4/2017, patrick van oostveldt wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>Post images on http://www.imgur.com and include the link in your posting.
>*****
>
>Is expansion microscopy in combination with
>selective plane illumination not an upcomming
>technique that also has superresolution performance?
>see:
>Expansion microscopy
>Fei Chen1,*, Paul W. Tillberg2,*, Edward S. Boyden1,3,4,5,6,†
>+ Author Affiliations
>↵†Corresponding author. E-mail: [hidden email]
>↵* These authors contributed equally to this work.
>Science  30 Jan 2015:
>Vol. 347, Issue 6221, pp. 543-548
>DOI: 10.1126/science.
>
>The fact that we increase resolution by specific
>swelling of the subject and this in combination
>with a library of specific antibodies certainly
>can create breakthroughs in e.g. neuroscience.
>
>Patrick Van Oostveldt
>Sint-Denijslaan 199
>9000 GENT
>
>Mobile [phone]
>Sent from my iPad
>
> > On 4 Jan 2017, at 17:43, Barbara Foster <[hidden email]> wrote:
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your posting.
> > *****
> >
> > Dear Listers,
> >
> > As follow-up to an article I wrote in 2011
> ("Superresolution: Reality or a "STORM" in a
> Teacup", BioPhotonics, Jan 2011 - PDF available
> at
> http://microscopyeducation.com/the-library/),
> I'm working with a colleague to create an
> educational poster on Super Resolution. Can you help with the following:
> > 1.     Did we miss any of the current "Key" techniques? (see list below)
> > 2.     Do you have a clear but simple
> explanatory diagram (PPT slide) of any of these techniques that you can share?
> > 3.     Do you have a gorgeous scientific
> image that represents that
> technique?  (especially valuable: comparison
> images that show widefield fluorescence/confocal/SR or something similar)
> > 4.     Historically, what are the key
> milestones (ex: 1873/Abbe Diffraction limit;
> 2004/STED becomes commercially available;
> 2006/PALM & STORM; 2014/Nobel Prize etc.)
> >
> > We will, of course, cite credits for all
> contributed materials.  Also, please remember
> that this will be just a poster.  Brevity will be important.
> >
> > Our target date: April (but we need to finish
> work this month to hit production deadlines)
> >
> > The poster will be available for FREE
> (Details will follow as we get closer to the print date)
> >
> > Proposed techniques to be covered:
> > ·         Nearfield microscopy
> > ·         RESOLFT/STED/GSD
> > ·         SIM
> > ·         PALM/STORM
> >          Video-rate, single-molecule localization microscopy
> >
> > Thanks, in advance for any help you can provide!
> > Barbara Foster, President & Chief Consultant
> > Microscopy/Microscopy Education  ... "Education, not Training"
> > 7101 Royal Glen Trail, Suite A  - McKinney, TX 75070 - P: 972-924-5310
> > www.MicroscopyEducation.com
> >
> > Microscopy/Microscopy Education is a division
> of The Microscopy & Imaging Place, Inc.

[Barbara Foster]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Many thanks, George.

-Barbara

At 01:24 PM 1/4/2017, George McNamara wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>Post images on http://www.imgur.com and include the link in your posting.
>*****
>
>Hi Barbara,
>
>Patrick v.L. already replied about Expansion Microscopy.
>
>More items:
>
>* "there's a fluorescent protein biosensor for
>that, and that, and that, and that too" ...
>http://www.geomcnamara.com/fluorescent-biosensors
>
>* current FPs are 3x brighter than EGFP (which
>is soooooo 1996), and can be multimerized to
>make even brighter, fairly current table is at
>http://www.geomcnamara.com/fluorescent-proteins-photophysics-data 
>(some month my web master and I will get around
>to adding mScarlet and other new FPs).
>
>* CRISPR/Cas9 (and homologs/orthologs) are hot.
>TALE-Lights
>(https://www.ncbi.nlm.nih.gov/pubmed/24556431),
>CRISPRrainbow etc ... see fig 4A of
>http://jcb.rupress.org/content/jcb/135/6/1685.full.pdf
>for starting point on this.
>
>* CyTOF imaging ... Garry Nolan / Michael Angelo
>and Bodenmiller labs (and check with Garry on "CODIS")
>
>* (brand new); high resolution (1.4 um laser
>spot size) atmospheric pressure MALDI MS imaging
>https://www.ncbi.nlm.nih.gov/pubmed/27842060
>
>* Xiaowei Zhuang (MERFISH), as well as Long Cai,
>and other labs, multiplex single cell RNAseq ...
>various ways to "error correct".
>
>* single molecule RNA FISHing can be very
>photogenic (even without AUCG's sequence info)
>... ask Ron Cook for copyright permission to
>image(s) from http://stellarisfish.smugmug.com/
>
>* you might find more topics from titles of talks and posters from QBI 2017,
>
>
>http://www.quantitativebioimaging.com/apps/schedule (days at top right)
>
>http://www.quantitativebioimaging.com/apps/schedule/posters.php
>
>* (not new - so awesome doesn't matter) You are
>welcome to include the best ever example of
>digital slide imaging: Tiki_Goddess
>http://home.earthlink.net/~tiki_goddess/TikiGoddess.jpg
>
>* microscopy as Q.C. for single cell NGS, for
>example the imaging station component of
>http://www.wafergen.com/products/icell8-single-cell-system 
>... Nicholas Navin at MD Anderson Cancer Center
>now has single cell RNAseq at ~$1/cell with this
>platform (not including data analysis or data storage).
>
>enjoy,
>
>George
>
>
>On 1/4/2017 10:43 AM, Barbara Foster wrote:
>>*****
>>To join, leave or search the confocal microscopy listserv, go to:
>>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>Post images on http://www.imgur.com and include the link in your posting.
>>*****
>>
>>Dear Listers,
>>
>>As follow-up to an article I wrote in 2011
>>(“Superresolution: Reality or a “STORM” in a
>>Teacup”, BioPhotonics, Jan 2011 - PDF available
>>at
>>http://microscopyeducation.com/the-library/),
>>I’m working with a colleague to create an
>>educational poster on Super Resolution. Can you help with the following:
>>1.     Did we miss any of the current “Key” techniques? (see list below)
>>2.     Do you have a clear but simple
>>explanatory diagram (PPT slide) of any of these techniques that you can share?
>>3.     Do you have a gorgeous scientific image
>>that represents that technique?  (especially
>>valuable: comparison images that show widefield
>>fluorescence/confocal/SR or something similar)
>>4.     Historically, what are the key
>>milestones (ex: 1873/Abbe Diffraction limit;
>>2004/STED becomes commercially available;
>>2006/PALM & STORM; 2014/Nobel Prize etc.)
>>
>>We will, of course, cite credits for all
>>contributed materials. Also, please remember
>>that this will be just a poster.  Brevity will be important.
>>
>>Our target date: April (but we need to finish
>>work this month to hit production deadlines)
>>
>>The poster will be available for FREE (Details
>>will follow as we get closer to the print date)
>>
>>Proposed techniques to be covered:
>>·         Nearfield microscopy
>>·         RESOLFT/STED/GSD
>>·         SIM
>>·         PALM/STORM
>>           Video-rate, single-molecule localization microscopy
>>
>>Thanks, in advance for any help you can provide!
>>Barbara Foster, President & Chief Consultant
>>Microscopy/Microscopy Education  ... "Education, not Training"
>>7101 Royal Glen Trail, Suite A  - McKinney, TX 75070 - P: 972-924-5310
>>www.MicroscopyEducation.com
>>
>>Microscopy/Microscopy Education is a division
>>of The Microscopy & Imaging Place, Inc.
>
>--
>
>
>George McNamara, PhD
>Houston, TX 77054
>[hidden email]
>https://www.linkedin.com/in/georgemcnamara
>https://works.bepress.com/gmcnamara/75/
>http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650

[Steffen Dietzel]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Barbara,

is a factor of 1.4 sufficient to count as superresolution? In any case,
with a bright and stable sample and a pinhole of 0.5 or so, you surely
can go beyond the Abbe limit. If you put deconvolution on top, even more
so. Leica Hyvolution and Zeiss Airy Scan are commercial packages making
use of this.

There is another early way to go beyond Abbe established in the 90ies
that is usually forgotten, spectral position distance measurements. If
two dots are labeled in different colors, with the proper correction
factors for chromatic aberration, distances were determined with an
accuracy <50 nm, even for close signals.

Then of course there was 4 Pi, but maybe that would be too much detail
for a poster.

Steffen


Am 04.01.2017 um 17:43 schrieb Barbara Foster:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Listers,
>
> As follow-up to an article I wrote in 2011 (“Superresolution: Reality
> or a “STORM” in a Teacup”, BioPhotonics, Jan 2011 - PDF available at
> http://microscopyeducation.com/the-library/), I’m working with a
> colleague to create an educational poster on Super Resolution. Can you
> help with the following:
> 1.     Did we miss any of the current “Key” techniques? (see list below)
> 2.     Do you have a clear but simple explanatory diagram (PPT slide)
> of any of these techniques that you can share?
> 3.     Do you have a gorgeous scientific image that represents that
> technique?  (especially valuable: comparison images that show
> widefield fluorescence/confocal/SR or something similar)
> 4.     Historically, what are the key milestones (ex: 1873/Abbe
> Diffraction limit; 2004/STED becomes commercially available; 2006/PALM
> & STORM; 2014/Nobel Prize etc.)
>
> We will, of course, cite credits for all contributed materials. Also,
> please remember that this will be just a poster.  Brevity will be
> important.
>
> Our target date: April (but we need to finish work this month to hit
> production deadlines)
>
> The poster will be available for FREE (Details will follow as we get
> closer to the print date)
>
> Proposed techniques to be covered:
> ·         Nearfield microscopy
> ·         RESOLFT/STED/GSD
> ·         SIM
> ·         PALM/STORM
>           Video-rate, single-molecule localization microscopy
>
> Thanks, in advance for any help you can provide!
> Barbara Foster, President & Chief Consultant
> Microscopy/Microscopy Education  ... "Education, not Training"
> 7101 Royal Glen Trail, Suite A  - McKinney, TX 75070 - P: 972-924-5310
> www.MicroscopyEducation.com
>
> Microscopy/Microscopy Education is a division of The Microscopy &
> Imaging Place, Inc.
>

--
-- ----------------------------------------------------------

Steffen Dietzel, PD Dr. rer. nat.
Head of the Core Facility Bioimaging at the Biomedical Center
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für Experimentelle Medizin

Address:
Biomedical Center
Großhaderner Straße 9
D-82152 Planegg-Martinsried

Phone: [phone]
skype: steffendietzel
e-mail: [hidden email]
fax-to-e-mail: +49/89/2180-9971518
http://www.bioimaging.bmc.med.uni-muenchen.de


--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging

Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany

http://www.bioimaging.bmc.med.uni-muenchen.de

[Barbara Foster]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Thanks, Steffan.  Anything below 100nm counts.

Actually, your description is the basis for both STORM and PALM!

Best regars,
Barbara
At 04:01 AM 1/9/2017, you wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>Post images on http://www.imgur.com and include the link in your posting.
>*****
>
>Barbara,
>
>is a factor of 1.4 sufficient to count as
>superresolution? In any case, with a bright and
>stable sample and a pinhole of 0.5 or so, you
>surely can go beyond the Abbe limit. If you put
>deconvolution on top, even more so. Leica
>Hyvolution and Zeiss Airy Scan are commercial packages making use of this.
>
>There is another early way to go beyond Abbe
>established in the 90ies that is usually
>forgotten, spectral position distance
>measurements. If two dots are labeled in
>different colors, with the proper correction
>factors for chromatic aberration, distances were
>determined with an accuracy <50 nm, even for close signals.
>
>Then of course there was 4 Pi, but maybe that
>would be too much detail for a poster.
>
>Steffen
>
>
>Am 04.01.2017 um 17:43 schrieb Barbara Foster:
>>*****
>>To join, leave or search the confocal microscopy listserv, go to:
>>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>Post images on http://www.imgur.com and include the link in your posting.
>>*****
>>
>>Dear Listers,
>>
>>As follow-up to an article I wrote in 2011 (“Superresolution: Reality
>>or a “STORM” in a Teacup”, BioPhotonics, Jan 2011 - PDF available at
>>http://microscopyeducation.com/the-library/), I’m working with a
>>colleague to create an educational poster on Super Resolution. Can you
>>help with the following:
>>1.     Did we miss any of the current “Key” techniques? (see list below)
>>2.     Do you have a clear but simple explanatory diagram (PPT slide)
>>of any of these techniques that you can share?
>>3.     Do you have a gorgeous scientific image that represents that
>>technique?  (especially valuable: comparison images that show
>>widefield fluorescence/confocal/SR or something similar)
>>4.     Historically, what are the key milestones (ex: 1873/Abbe
>>Diffraction limit; 2004/STED becomes commercially available; 2006/PALM
>>& STORM; 2014/Nobel Prize etc.)
>>
>>We will, of course, cite credits for all contributed materials. Also,
>>please remember that this will be just a poster.  Brevity will be
>>important.
>>
>>Our target date: April (but we need to finish work this month to hit
>>production deadlines)
>>
>>The poster will be available for FREE (Details will follow as we get
>>closer to the print date)
>>
>>Proposed techniques to be covered:
>>·         Nearfield microscopy
>>·         RESOLFT/STED/GSD
>>·         SIM
>>·         PALM/STORM
>>           Video-rate, single-molecule localization microscopy
>>
>>Thanks, in advance for any help you can provide!
>>Barbara Foster, President & Chief Consultant
>>Microscopy/Microscopy Education  ... "Education, not Training"
>>7101 Royal Glen Trail, Suite A  - McKinney, TX 75070 - P: 972-924-5310
>>www.MicroscopyEducation.com
>>
>>Microscopy/Microscopy Education is a division of The Microscopy &
>>Imaging Place, Inc.
>
>--
>-- ----------------------------------------------------------
>
>Steffen Dietzel, PD Dr. rer. nat.
>Head of the Core Facility Bioimaging at the Biomedical Center
>Ludwig-Maximilians-Universität München
>Walter-Brendel-Zentrum für Experimentelle Medizin
>
>Address:
>Biomedical Center
>Großhaderner Straße 9
>D-82152 Planegg-Martinsried
>
>Phone: [phone]
>skype: steffendietzel
>e-mail: [hidden email]
>fax-to-e-mail: +49/89/2180-9971518
>http://www.bioimaging.bmc.med.uni-muenchen.de
>
>
>--
>------------------------------------------------------------
>Steffen Dietzel, PD Dr. rer. nat
>Ludwig-Maximilians-Universität München
>Biomedical Center (BMC)
>Head of the Core Facility Bioimaging
>
>Großhaderner Straße 9
>D-82152 Planegg-Martinsried
>Germany
>
>http://www.bioimaging.bmc.med.uni-muenchen.de

[jerie]

*****
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Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Barbara,

Re-scan Confocal Microscopy (
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829557/), a practical and
commercially available implementation of the more than 20 year old Image
Scanning Microscopy should qualify for your poster.
We took very impressive images during a recent visit at Erik Manders
laboratories and confocal.nl company (no commercial interest).

Kind regards, Jens


On 4 Jan 2017 5:47 p.m., "Barbara Foster" <[hidden email]> wrote:

*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Listers,

As follow-up to an article I wrote in 2011 (“Superresolution: Reality or a
“STORM” in a Teacup”, BioPhotonics, Jan 2011 - PDF available at
http://microscopyeducation.com/the-library/), I’m working with a colleague
to create an educational poster on Super Resolution. Can you help with the
following:
1.     Did we miss any of the current “Key” techniques? (see list below)
2.     Do you have a clear but simple explanatory diagram (PPT slide) of
any of these techniques that you can share?
3.     Do you have a gorgeous scientific image that represents that
technique?  (especially valuable: comparison images that show widefield
fluorescence/confocal/SR or something similar)
4.     Historically, what are the key milestones (ex: 1873/Abbe Diffraction
limit; 2004/STED becomes commercially available; 2006/PALM & STORM;
2014/Nobel Prize etc.)

We will, of course, cite credits for all contributed materials.  Also,
please remember that this will be just a poster.  Brevity will be important.

Our target date: April (but we need to finish work this month to hit
production deadlines)

The poster will be available for FREE (Details will follow as we get closer
to the print date)

Proposed techniques to be covered:
·         Nearfield microscopy
·         RESOLFT/STED/GSD
·         SIM
·         PALM/STORM
          Video-rate, single-molecule localization microscopy

Thanks, in advance for any help you can provide!
Barbara Foster, President & Chief Consultant
Microscopy/Microscopy Education  ... "Education, not Training"
7101 Royal Glen Trail, Suite A  - McKinney, TX 75070 - P: 972-924-5310
www.MicroscopyEducation.com

Microscopy/Microscopy Education is a division of The Microscopy & Imaging
Place, Inc.

[John Oreopoulos]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Barbara,

Looking forward to the end result. I just wanted to say that to me it seems there is an ever growing list of super-resolution imaging technique variants, so much so that it's very difficult to keep up with that list. There has literally been an explosion in this field and every week or two my citation feed seems to have at least one paper describing a new twist on super-resolution. You may end up having to make another poster in a year or two.

I would hope that your poster pays tribute to the growing number of what would be considered fluctuation and computational based super-resolution methods, such as 3B analysis, SOFI, or SRRF. These methods are interesting in that they don't really require any new hardware or special probes, the main cost being time to acquire data and computational power to analyze the data.

Cheers,

John Oreopoulos


On 2017-01-11, at 1:56 AM, jens rietdorf wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Barbara,
>
> Re-scan Confocal Microscopy (
> https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829557/), a practical and
> commercially available implementation of the more than 20 year old Image
> Scanning Microscopy should qualify for your poster.
> We took very impressive images during a recent visit at Erik Manders
> laboratories and confocal.nl company (no commercial interest).
>
> Kind regards, Jens
>
>
> On 4 Jan 2017 5:47 p.m., "Barbara Foster" <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Listers,
>
> As follow-up to an article I wrote in 2011 (“Superresolution: Reality or a
> “STORM” in a Teacup”, BioPhotonics, Jan 2011 - PDF available at
> http://microscopyeducation.com/the-library/), I’m working with a colleague
> to create an educational poster on Super Resolution. Can you help with the
> following:
> 1.     Did we miss any of the current “Key” techniques? (see list below)
> 2.     Do you have a clear but simple explanatory diagram (PPT slide) of
> any of these techniques that you can share?
> 3.     Do you have a gorgeous scientific image that represents that
> technique?  (especially valuable: comparison images that show widefield
> fluorescence/confocal/SR or something similar)
> 4.     Historically, what are the key milestones (ex: 1873/Abbe Diffraction
> limit; 2004/STED becomes commercially available; 2006/PALM & STORM;
> 2014/Nobel Prize etc.)
>
> We will, of course, cite credits for all contributed materials.  Also,
> please remember that this will be just a poster.  Brevity will be important.
>
> Our target date: April (but we need to finish work this month to hit
> production deadlines)
>
> The poster will be available for FREE (Details will follow as we get closer
> to the print date)
>
> Proposed techniques to be covered:
> ·         Nearfield microscopy
> ·         RESOLFT/STED/GSD
> ·         SIM
> ·         PALM/STORM
>          Video-rate, single-molecule localization microscopy
>
> Thanks, in advance for any help you can provide!
> Barbara Foster, President & Chief Consultant
> Microscopy/Microscopy Education  ... "Education, not Training"
> 7101 Royal Glen Trail, Suite A  - McKinney, TX 75070 - P: 972-924-5310
> www.MicroscopyEducation.com
>
> Microscopy/Microscopy Education is a division of The Microscopy & Imaging
> Place, Inc.

[Roland Nitschke]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear List,

Final Call for

Core Facility Management Software Workshop

7th and 8th of February 2017, in Freiburg, Germany

The workshop held from Tuesday 07.02. to Wednesday 08.02.2017 is
primarily designed to meet the requirements of core facility managers,
looking for the optimal solution, as well as team or IT members, who
will have to setup such a management software in their home
institutions. We also encourage major opinion leaders in the field to
use this workshop as an opportunity to discuss and define the future
needs in the field.
Please find the workshop program and registration form on the following
webpage:

http://www.imaging.uni-freiburg.de/

Extended Deadline for registration: January, 20th 2017.

The registration fee of 50 € covers finger food and refreshments during
the workshop.


Travel Information:

EuroAirport Basel, Mulhouse, Freiburg (BSL) -75km. The closest and most
convenient Airport (approx. 1 hour bus ride):

http://www.euroairport.com/en/flights/scheduled-destinations.html

Strasbourg airport (SXB) -80km. Small but also good to reach from
Freiburg (approx. 1 hour bus ride):

http://strasbourg.aeroport.fr/EN/Passengers/Flights/Destination-map.html

Frankfurt International Airport (FRA), Stuttgart (STR) and Zürich (ZRH):
2 hours by train or car.

If you are interested in an individual software demonstration with one
of the companies, please contact us at:
[hidden email] or indicate it on the registration form and
we will forward your request.

We hope to see you soon in Freiburg.

Sylvia Olberg, Jürgen Reymann and Roland Nitschke
(The MIAP Coordination Team)

___________________________

Albert-Ludwigs-University Freiburg
Life Imaging Center (LIC) in ZBSA
Dr. Roland Nitschke
Habsburgerstr.49
D-79104 Freiburg
Germany
___________________________
E-mail:[hidden email]
phone: [phone] or 2902
fax: [phone]
http://www.imaging.uni-freiburg.de/

[George McNamara]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Barbara,

Wrt YCNHEA (you can never have enough acronyms:

SPEED ...
https://www.ncbi.nlm.nih.gov/pubmed/?term=Single+Point+Edge-Excitation+Subdiffraction+microscopy 
(similar to HiLo)

SPINDLE ... http://doublehelixoptics.com/index.php/products 
(commercialization of Double Helix Point Spread Function)

smFRET and sptPALM ... http://www.oxfordni.com/product/the-nanoimager

P-PALM ... polarization PALM (either linear or circular ... one of the
talks at QBI)

There may be some more acronyms and useful techniques in the abstracts
and titles of the QBI conference at TAMU last week

http://www.quantitativebioimaging.com/files/QBI_2017_booklet.pdf

such as "SQUBIC":

Chrysanthe Preza University of Memphis, Memphis, USA.
Implementation of PSF engineering using a fabricated SQUBIC phase mask
to reduce the effect of spherical aberration in 3D wide field
fluorescence imaging [ABSTRACT # 52]

cryoSTED (2.0):

Robert Moerland, Christiaan Hulleman, Sjoerd Stallinga, Bernd Rieger

Inducing sparsity AT LN2 temperatures: towards polarization‐selective
single molecule excitation and emission depletion [abstract #44]

TSUNAMI:

Studying T7 phage particle transport in artificial mucus by TSUNAMI 3D
tracking microscopy

Yuan‐I Chen, Jasmim Leal, Yen‐Liang Liu, Debadyuti Ghosh, Tim Yeh
[abstract #79]

(gm note: several abstracts/posters at QBI that bacteriophage have
"coopetition" = cooperatation and/or competetion)

STREM:

Super temporal‐resolved microscopy (STReM)

Wenxiao Wang, Hao Shen, Bo Shuang, Benjamin Hoener, Lawrence Tauzin,
Nicholas Moringo, Kevin Kelly, Christy Landes

//

an important take-home for me from QBI is that the camera manufacturers
know more about their cameras than the academics (and are starting to do
the corrections before the data reaches the user's application). Keith
Bennett, a consultant for Hamamatsu, had an excellent poster and has
already send me the PDF. I encourage anyone interested in obtaining
optimal images to ask Keith for the QBI poster and to ask the camera
manufacturer(s) what they are doing.

Keith Bennett Hamamatsu Photonics K.K., Hamamatsu, Japan.
Quantitative evalutation of accuracy and variance of individual pixels
in a Scientific CMOS (SCMOS) camera for computational imaging [ABSTRACT
# 30]



Enjoy,

George
p.s. with respect to Oxford Nanoimaging and Double Helix, I think either
could be outstanding platforms for single molecule FRET FP biosensors.
For brighter signal to noise, I suggest localizing the FRET biosensors
to small spots. For example, the glucose biosensor could be attached to
GLUT1 or GLUT4, the lactate biosensor to the MCT transporter and/or the
GPR132 GPCR that is its sensor in tumor microenvironment (recent PNAS
paper on the latter). Table of FP biosensors is at
http://www.geomcnamara.com/fluorescent-biosensors

FP photophysics  table at
http://www.geomcnamara.com/fluorescent-proteins-photophysics-data
(mScarlet is not in there yet, the iRFPs etc are ... column header click
to sort).




On 1/11/2017 1:14 AM, John Oreopoulos wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Barbara,
>
> Looking forward to the end result. I just wanted to say that to me it seems there is an ever growing list of super-resolution imaging technique variants, so much so that it's very difficult to keep up with that list. There has literally been an explosion in this field and every week or two my citation feed seems to have at least one paper describing a new twist on super-resolution. You may end up having to make another poster in a year or two.
>
> I would hope that your poster pays tribute to the growing number of what would be considered fluctuation and computational based super-resolution methods, such as 3B analysis, SOFI, or SRRF. These methods are interesting in that they don't really require any new hardware or special probes, the main cost being time to acquire data and computational power to analyze the data.
>
> Cheers,
>
> John Oreopoulos
>
>
> On 2017-01-11, at 1:56 AM, jens rietdorf wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Dear Barbara,
>>
>> Re-scan Confocal Microscopy (
>> https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829557/), a practical and
>> commercially available implementation of the more than 20 year old Image
>> Scanning Microscopy should qualify for your poster.
>> We took very impressive images during a recent visit at Erik Manders
>> laboratories and confocal.nl company (no commercial interest).
>>
>> Kind regards, Jens
>>
>>
>> On 4 Jan 2017 5:47 p.m., "Barbara Foster" <[hidden email]> wrote:
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Dear Listers,
>>
>> As follow-up to an article I wrote in 2011 (“Superresolution: Reality or a
>> “STORM” in a Teacup”, BioPhotonics, Jan 2011 - PDF available at
>> http://microscopyeducation.com/the-library/), I’m working with a colleague
>> to create an educational poster on Super Resolution. Can you help with the
>> following:
>> 1.     Did we miss any of the current “Key” techniques? (see list below)
>> 2.     Do you have a clear but simple explanatory diagram (PPT slide) of
>> any of these techniques that you can share?
>> 3.     Do you have a gorgeous scientific image that represents that
>> technique?  (especially valuable: comparison images that show widefield
>> fluorescence/confocal/SR or something similar)
>> 4.     Historically, what are the key milestones (ex: 1873/Abbe Diffraction
>> limit; 2004/STED becomes commercially available; 2006/PALM & STORM;
>> 2014/Nobel Prize etc.)
>>
>> We will, of course, cite credits for all contributed materials.  Also,
>> please remember that this will be just a poster.  Brevity will be important.
>>
>> Our target date: April (but we need to finish work this month to hit
>> production deadlines)
>>
>> The poster will be available for FREE (Details will follow as we get closer
>> to the print date)
>>
>> Proposed techniques to be covered:
>> ·         Nearfield microscopy
>> ·         RESOLFT/STED/GSD
>> ·         SIM
>> ·         PALM/STORM
>>           Video-rate, single-molecule localization microscopy
>>
>> Thanks, in advance for any help you can provide!
>> Barbara Foster, President & Chief Consultant
>> Microscopy/Microscopy Education  ... "Education, not Training"
>> 7101 Royal Glen Trail, Suite A  - McKinney, TX 75070 - P: 972-924-5310
>> www.MicroscopyEducation.com
>>
>> Microscopy/Microscopy Education is a division of The Microscopy & Imaging
>> Place, Inc.

--


George McNamara, PhD
Houston, TX 77054
[hidden email]
https://www.linkedin.com/in/georgemcnamara
https://works.bepress.com/gmcnamara/75/
http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650

[Andrew York]

*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

 I want to echo Jens Rietdorf; I'm very impressed with rescan confocal
microscopy. I'd also like to emphasize, re-scan confocal microscopy is an
additional innovation, which was not described in Sheppard's 25-year-old
publication ( https://goo.gl/sGupqr ), or anywhere else, when Manders and
De Luca conceived their idea ( https://goo.gl/9WHfhr ).

 Swept-field confocal microscopy existed for more than 20 years (
https://goo.gl/T7V7Tj ) with several commercial implementations (e.g.
Bruker, Visitech). I'm confident that if anyone had realized between 1988
and 2013 you could get SIM-like resolution by adding a single optic to the
emission path of a swept-field or spinning disk confocal (
https://goo.gl/14P4bV , https://goo.gl/HtDi1i ), they wouldn't have kept it
quiet.

On Tue, Jan 10, 2017 at 10:56 PM, jens rietdorf <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Barbara,
>
> Re-scan Confocal Microscopy (
> https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829557/), a practical and
> commercially available implementation of the more than 20 year old Image
> Scanning Microscopy should qualify for your poster.
> We took very impressive images during a recent visit at Erik Manders
> laboratories and confocal.nl company (no commercial interest).
>
> Kind regards, Jens
>
>
> On 4 Jan 2017 5:47 p.m., "Barbara Foster" <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Listers,
>
> As follow-up to an article I wrote in 2011 (“Superresolution: Reality or a
> “STORM” in a Teacup”, BioPhotonics, Jan 2011 - PDF available at
> http://microscopyeducation.com/the-library/), I’m working with a colleague
> to create an educational poster on Super Resolution. Can you help with the
> following:
> 1.     Did we miss any of the current “Key” techniques? (see list below)
> 2.     Do you have a clear but simple explanatory diagram (PPT slide) of
> any of these techniques that you can share?
> 3.     Do you have a gorgeous scientific image that represents that
> technique?  (especially valuable: comparison images that show widefield
> fluorescence/confocal/SR or something similar)
> 4.     Historically, what are the key milestones (ex: 1873/Abbe Diffraction
> limit; 2004/STED becomes commercially available; 2006/PALM & STORM;
> 2014/Nobel Prize etc.)
>
> We will, of course, cite credits for all contributed materials.  Also,
> please remember that this will be just a poster.  Brevity will be
> important.
>
> Our target date: April (but we need to finish work this month to hit
> production deadlines)
>
> The poster will be available for FREE (Details will follow as we get closer
> to the print date)
>
> Proposed techniques to be covered:
> ·         Nearfield microscopy
> ·         RESOLFT/STED/GSD
> ·         SIM
> ·         PALM/STORM
>           Video-rate, single-molecule localization microscopy
>
> Thanks, in advance for any help you can provide!
> Barbara Foster, President & Chief Consultant
> Microscopy/Microscopy Education  ... "Education, not Training"
> 7101 Royal Glen Trail, Suite A  - McKinney, TX 75070 - P: 972-924-5310
> www.MicroscopyEducation.com
>
> Microscopy/Microscopy Education is a division of The Microscopy & Imaging
> Place, Inc.
>