New microscope objectives

[Adrian Negrean]

Hi all,

Does anybody know what are the specs for the new Olympus 25x 1.05 N.A.
objective or the Nikon 25x 1.1 N.A. objective ?

More specifically:

- What is the transmission of the objective up to 1400 nm ?
- From what I know all Olympus objectives are designed to be used with a 180
mm tube lens. Is this also for this Olympus objective ? Then the effective
focal length of the objective would be 7.2 mm.
- What is the Field Number of the objective ? Suppose this is 22 mm like for
the 20x 1.0 NA, is it correct then that the diameter of the image plane of
the sample for which the objective is designed is 22/25 mm=0.88 mm.
- What is the diameter of the back-aperture that has to be overfilled with
the laser beam to get the best PSF ? I intend to overfill it with a
truncation ratio of 1.
- What is the location of the paraxial lens equivalent of the objective
within the objective ?
- Is the thread type of this objective the same as for the 20x 1.0 N.A. ?

Did anybody work with these objectives so far ? What is your experience ?

Thanks!

[Cameron Nowell]

Hi Adrian,

We have one of the new Olympus 25x 1.05NA objective installed. In short
it is absolutely amazing. The image quality, s/n ratio etc. compared to
our older 20x 0.95NA is phenomenally better. We have reduced excitation
power by about 1/2 to 2/3 on our regular samples (zebrafish expressing
GFP) @920nm. The resolution while technically only slightly improved
looks a hell of a lot better. The correction collar is also a major
advantage as we can now compensate for the embedding media of out
samples some what.

I can answer some, but not all of your questions.

- Transmission of the objective is meant to be ~82% out to 1000nm, not
sure what it is at 1400nm but since out MaiTai doesn't tune that far I
haven't had to find out.
- The field number is 27.5. This is achieved by creating a "virtual"
back plane inside the microscope somewhere. The field is not any larger
than the 20x though due to the system being limited to 18mm FN (though
this could be higher in the current generation FV1000 MPE systems that
have an adaptable excitation path).
- Not sure about the overfill question, hopefully Olympus can chip in
with that answer.
- The thread is identical to the older 20x lens. So if you want a bigger
filed you can always swap them over (assuming you have the adaptable
light path, otherwise you will have reduced performance.)

Cheers

Cam



Cameron J. Nowell
Microscopy Manager
Centre for Advanced Microscopy
Ludwig Institute for Cancer Research
PO Box 2008
Royal Melbourne Hospital
Victoria, 3050
AUSTRALIA
Office: [phone]
Mobile: [phone]
Fax: [phone]
Facility Website


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Adrian Negrean
Sent: Sunday, 22 August 2010 11:17 PM
To: [hidden email]
Subject: New microscope objectives

Hi all,

Does anybody know what are the specs for the new Olympus 25x 1.05 N.A.
objective or the Nikon 25x 1.1 N.A. objective ?

More specifically:

- What is the transmission of the objective up to 1400 nm ?
- From what I know all Olympus objectives are designed to be used with a
180
mm tube lens. Is this also for this Olympus objective ? Then the
effective
focal length of the objective would be 7.2 mm.
- What is the Field Number of the objective ? Suppose this is 22 mm like
for
the 20x 1.0 NA, is it correct then that the diameter of the image plane
of
the sample for which the objective is designed is 22/25 mm=0.88 mm.
- What is the diameter of the back-aperture that has to be overfilled
with
the laser beam to get the best PSF ? I intend to overfill it with a
truncation ratio of 1.
- What is the location of the paraxial lens equivalent of the objective
within the objective ?
- Is the thread type of this objective the same as for the 20x 1.0 N.A.
?

Did anybody work with these objectives so far ? What is your experience
?

Thanks!
--
View this message in context:
http://confocal-microscopy-list.588098.n2.nabble.com/New-microscope-obje
ctives-tp5449641p5449641.html
Sent from the Confocal Microscopy List mailing list archive at
Nabble.com.


This communication is intended only for the named recipient and may contain information that is confidential, legally privileged or subject to copyright; the Ludwig Institute for Cancer Research Ltd does not waive any rights if you have received this communication in error.
The views expressed in this communication are those of the sender and do not necessarily reflect the views of the Ludwig Institute for Cancer Research Ltd.

[Adrian Smith-6]

Hi all,

Does anyone know if Olympus are still only selling that objective with Olympus MPE systems or have they opened it up to anyone to buy?

(Nikon will sell theirs to anyone :) (but I haven't heard any feedback from users yet)).

Regards,

Adrian

On 23/08/2010, at 9:36 AM, Cameron Nowell wrote:

> Hi Adrian,
>
> We have one of the new Olympus 25x 1.05NA objective installed. In short
> it is absolutely amazing. The image quality, s/n ratio etc. compared to
> our older 20x 0.95NA is phenomenally better. We have reduced excitation
> power by about 1/2 to 2/3 on our regular samples (zebrafish expressing
> GFP) @920nm. The resolution while technically only slightly improved
> looks a hell of a lot better. The correction collar is also a major
> advantage as we can now compensate for the embedding media of out
> samples some what.
>
> I can answer some, but not all of your questions.
>
> - Transmission of the objective is meant to be ~82% out to 1000nm, not
> sure what it is at 1400nm but since out MaiTai doesn't tune that far I
> haven't had to find out.
> - The field number is 27.5. This is achieved by creating a "virtual"
> back plane inside the microscope somewhere. The field is not any larger
> than the 20x though due to the system being limited to 18mm FN (though
> this could be higher in the current generation FV1000 MPE systems that
> have an adaptable excitation path).
> - Not sure about the overfill question, hopefully Olympus can chip in
> with that answer.
> - The thread is identical to the older 20x lens. So if you want a bigger
> filed you can always swap them over (assuming you have the adaptable
> light path, otherwise you will have reduced performance.)
>
> Cheers
>
> Cam
>
>
>
> Cameron J. Nowell
> Microscopy Manager
> Centre for Advanced Microscopy
> Ludwig Institute for Cancer Research
> PO Box 2008
> Royal Melbourne Hospital
> Victoria, 3050
> AUSTRALIA
> Office: [phone]
> Mobile: [phone]
> Fax: [phone]
> Facility Website
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Adrian Negrean
> Sent: Sunday, 22 August 2010 11:17 PM
> To: [hidden email]
> Subject: New microscope objectives
>
> Hi all,
>
> Does anybody know what are the specs for the new Olympus 25x 1.05 N.A.
> objective or the Nikon 25x 1.1 N.A. objective ?
>
> More specifically:
>
> - What is the transmission of the objective up to 1400 nm ?
> - From what I know all Olympus objectives are designed to be used with a
> 180
> mm tube lens. Is this also for this Olympus objective ? Then the
> effective
> focal length of the objective would be 7.2 mm.
> - What is the Field Number of the objective ? Suppose this is 22 mm like
> for
> the 20x 1.0 NA, is it correct then that the diameter of the image plane
> of
> the sample for which the objective is designed is 22/25 mm=0.88 mm.
> - What is the diameter of the back-aperture that has to be overfilled
> with
> the laser beam to get the best PSF ? I intend to overfill it with a
> truncation ratio of 1.
> - What is the location of the paraxial lens equivalent of the objective
> within the objective ?
> - Is the thread type of this objective the same as for the 20x 1.0 N.A.
> ?
>
> Did anybody work with these objectives so far ? What is your experience
> ?
>
> Thanks!
> --
> View this message in context:
> http://confocal-microscopy-list.588098.n2.nabble.com/New-microscope-obje
> ctives-tp5449641p5449641.html
> Sent from the Confocal Microscopy List mailing list archive at
> Nabble.com.
>
>
> This communication is intended only for the named recipient and may contain information that is confidential, legally privileged or subject to copyright; the Ludwig Institute for Cancer Research Ltd does not waive any rights if you have received this communication in error.
> The views expressed in this communication are those of the sender and do not necessarily reflect the views of the Ludwig Institute for Cancer Research Ltd.

[Cameron Nowell]

My understanding is that it only comes with the new MPE system, but ours
is fitted to an older generation one.



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Adrian Smith
Sent: Monday, 23 August 2010 10:13 AM
To: [hidden email]
Subject: Re: New microscope objectives

Hi all,

Does anyone know if Olympus are still only selling that objective with
Olympus MPE systems or have they opened it up to anyone to buy?

(Nikon will sell theirs to anyone :) (but I haven't heard any feedback
from users yet)).

Regards,

Adrian

On 23/08/2010, at 9:36 AM, Cameron Nowell wrote:

> Hi Adrian,
>
> We have one of the new Olympus 25x 1.05NA objective installed. In
short
> it is absolutely amazing. The image quality, s/n ratio etc. compared
to
> our older 20x 0.95NA is phenomenally better. We have reduced
excitation

> power by about 1/2 to 2/3 on our regular samples (zebrafish expressing
> GFP) @920nm. The resolution while technically only slightly improved
> looks a hell of a lot better. The correction collar is also a major
> advantage as we can now compensate for the embedding media of out
> samples some what.
>
> I can answer some, but not all of your questions.
>
> - Transmission of the objective is meant to be ~82% out to 1000nm, not
> sure what it is at 1400nm but since out MaiTai doesn't tune that far I
> haven't had to find out.
> - The field number is 27.5. This is achieved by creating a "virtual"
> back plane inside the microscope somewhere. The field is not any

larger
> than the 20x though due to the system being limited to 18mm FN (though
> this could be higher in the current generation FV1000 MPE systems that
> have an adaptable excitation path).
> - Not sure about the overfill question, hopefully Olympus can chip in
> with that answer.
> - The thread is identical to the older 20x lens. So if you want a
bigger

> filed you can always swap them over (assuming you have the adaptable
> light path, otherwise you will have reduced performance.)
>
> Cheers
>
> Cam
>
>
>
> Cameron J. Nowell
> Microscopy Manager
> Centre for Advanced Microscopy
> Ludwig Institute for Cancer Research
> PO Box 2008
> Royal Melbourne Hospital
> Victoria, 3050
> AUSTRALIA
> Office: [phone]
> Mobile: [phone]
> Fax: [phone]
> Facility Website
>
>
> -----Original Message-----
> From: Confocal Microscopy List

[mailto:[hidden email]]

> On Behalf Of Adrian Negrean
> Sent: Sunday, 22 August 2010 11:17 PM
> To: [hidden email]
> Subject: New microscope objectives
>
> Hi all,
>
> Does anybody know what are the specs for the new Olympus 25x 1.05 N.A.
> objective or the Nikon 25x 1.1 N.A. objective ?
>
> More specifically:
>
> - What is the transmission of the objective up to 1400 nm ?
> - From what I know all Olympus objectives are designed to be used with

a
> 180
> mm tube lens. Is this also for this Olympus objective ? Then the
> effective
> focal length of the objective would be 7.2 mm.
> - What is the Field Number of the objective ? Suppose this is 22 mm
like
> for
> the 20x 1.0 NA, is it correct then that the diameter of the image
plane
> of
> the sample for which the objective is designed is 22/25 mm=0.88 mm.
> - What is the diameter of the back-aperture that has to be overfilled
> with
> the laser beam to get the best PSF ? I intend to overfill it with a
> truncation ratio of 1.
> - What is the location of the paraxial lens equivalent of the
objective
> within the objective ?
> - Is the thread type of this objective the same as for the 20x 1.0
N.A.
> ?
>
> Did anybody work with these objectives so far ? What is your
experience
> ?
>
> Thanks!
> --
> View this message in context:
>
http://confocal-microscopy-list.588098.n2.nabble.com/New-microscope-obje
> ctives-tp5449641p5449641.html
> Sent from the Confocal Microscopy List mailing list archive at
> Nabble.com.
>
>
> This communication is intended only for the named recipient and may
contain information that is confidential, legally privileged or subject
to copyright; the Ludwig Institute for Cancer Research Ltd does not
waive any rights if you have received this communication in error.
> The views expressed in this communication are those of the sender and
do not necessarily reflect the views of the Ludwig Institute for Cancer
Research Ltd.


This communication is intended only for the named recipient and may contain information that is confidential, legally privileged or subject to copyright; the Ludwig Institute for Cancer Research Ltd does not waive any rights if you have received this communication in error.
The views expressed in this communication are those of the sender and do not necessarily reflect the views of the Ludwig Institute for Cancer Research Ltd.

[Gavin Rumbaugh]

I concur with the previous poster on the amazing quality of the new Olympus Super 25x. We use it for multiphoton excitation of eGFP in brain tissue, and we are using the new FV1000MPE light path with the DeepSEE laser. The efficiency of photon collection is outstanding, and the clarity of images is like no other objective I have seen. Using GFP-M mice, we only need 7-10 mW at the sample for excellent s/n of dendritic spines...

The back aperture is approx 25mm and overfill can only be achieved using the new light path (according to Olympus).

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Adrian Negrean
Sent: Sunday, August 22, 2010 9:17 AM
To: [hidden email]
Subject: New microscope objectives

Hi all,

Does anybody know what are the specs for the new Olympus 25x 1.05 N.A.
objective or the Nikon 25x 1.1 N.A. objective ?

More specifically:

- What is the transmission of the objective up to 1400 nm ?
- From what I know all Olympus objectives are designed to be used with a 180
mm tube lens. Is this also for this Olympus objective ? Then the effective
focal length of the objective would be 7.2 mm.
- What is the Field Number of the objective ? Suppose this is 22 mm like for
the 20x 1.0 NA, is it correct then that the diameter of the image plane of
the sample for which the objective is designed is 22/25 mm=0.88 mm.
- What is the diameter of the back-aperture that has to be overfilled with
the laser beam to get the best PSF ? I intend to overfill it with a
truncation ratio of 1.
- What is the location of the paraxial lens equivalent of the objective
within the objective ?
- Is the thread type of this objective the same as for the 20x 1.0 N.A. ?

Did anybody work with these objectives so far ? What is your experience ?

Thanks!
--
View this message in context: http://confocal-microscopy-list.588098.n2.nabble.com/New-microscope-objectives-tp5449641p5449641.html
Sent from the Confocal Microscopy List mailing list archive at Nabble.com.